BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	Xen14_202	341	A→G	intergenic (–/–)	– / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Xen14_202	341	0	A	G	90.3%	895.9 / 60.2	443	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand): ref base A (13/30); new base G (173/227); total (186/257)
Fisher's exact test for biased strand distribution p-value = 1.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 444 total aligned reads displayed.

GATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACAGCGCGTATACGATGAAAATCACAAGGTATACGGTGTGCGTAAAGTCTGGCGTCAGTTGTTACGGGAAGGTATCAGAGTGGCCAGATGCACTGTGGCACGTCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  Xen14_202/192‑421

    aaCGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACAGCGCGTATACGATGAAAATCACAAGGTATACGGTGTGCGTAAAGTCTGGCGTCAGTTGTTACGGGAAGGTATCAGAGTGGCCAGATGCACTGTGGCACGtc                                                                                             <  1:3656884/150‑1 (MQ=255)

       gCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCa                                                                                          >  1:637576/1‑150 (MQ=255)

          gTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATgg                                                                                       <  1:3481789/150‑1 (MQ=255)

          gTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATgg                                                                                       >  1:1144277/1‑150 (MQ=255)

           tGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGc                                                                                      >  1:3464160/1‑150 (MQ=255)

           tGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGc                                                                                      >  1:149695/1‑150 (MQ=255)

           tGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGc                                                                                      <  1:3800727/150‑1 (MQ=255)

           tGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGc                                                                                      <  1:76828/150‑1 (MQ=255)

            gtccGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCg                                                                                     >  1:2622441/3‑150 (MQ=255)

            gCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGCAGCCAGATGTACAGTGGCGCGCCTCATGGCg                                                                                     >  1:1042915/1‑150 (MQ=255)

             cccGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACAGCGCGTATACGATGAAAATCACAAGGTATACGGTGTGCGTAAAGTCTGGCGTCAGTTGTTACGGGAAGGTATCAGAGTGGCCAGATGCACTGTGGCACGTCTCATGGCgg                                                                                    <  1:913278/150‑1 (MQ=255)

              ccgagcgcAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCGTGGCGGt                                                                                   <  1:55488/146‑1 (MQ=255)

              ccGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCCGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGt                                                                                   <  1:1535861/150‑1 (MQ=255)

                gTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTa                                                                                 <  1:2062136/150‑1 (MQ=255)

                    gcAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATggg                                                                             <  1:102421/150‑1 (MQ=255)

                    gcAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATggg                                                                             <  1:2349754/150‑1 (MQ=255)

                       gcgcGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACt                                                                          <  1:2645026/150‑1 (MQ=255)

                             tGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCgg                                                                    >  1:2555417/1‑150 (MQ=255)

                             tGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCgg                                                                    <  1:1913629/150‑1 (MQ=255)

                             tGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCgg                                                                    <  1:431556/150‑1 (MQ=255)

                               aCTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGgtg                                                                  <  1:1901149/150‑1 (MQ=255)

                               aCTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGgtg                                                                  >  1:281888/1‑150 (MQ=255)

                                 tGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGtt                                                                >  1:1829889/1‑150 (MQ=255)

                                  ggCTGAAGGAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTtc                                                               <  1:3665502/150‑1 (MQ=255)

                                  ggCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTtc                                                               >  1:1115076/1‑150 (MQ=255)

                                   gCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTtct                                                              <  1:1240033/150‑1 (MQ=255)

                                   gCTGAAGAAAGAGATACAGCGCGTATACGATGAAAATCACAAGGTATACGGTGTGCGTAAAGTCTGGCGTCAGTTGTTACGGGAAGGTATCAGAGTGGCCAGATGCACTGTGGCACGTCTCATGGCGGTTATGGGACTTGCCGGTGTtct                                                              >  1:1452297/1‑150 (MQ=255)

                                    tctgatttcaggcgttgggtgtggatcactattgcaccgttcgtgacaGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTtctc                                                             <  1:2381281‑M2/102‑1 (MQ=255)

                                     tGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTcc                                                            <  1:955775/150‑1 (MQ=255)

                                      gaagaaAGAGATACTGCGCGTATACGATGGGGATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCg                                                           >  1:2955868/1‑150 (MQ=255)

                                       aagaaagaGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCgg                                                          >  1:402814/1‑150 (MQ=25)

                                       aagaaagaGATACAGCGCGTATACGATGAAAATCACAAGGTATACGGTGTGCGTAAAGTCTGGCGTCAGTTGTTACGGGAAGGTATCAGAGTGGCCAGATGCACTGTGGCACGTCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCgg                                                          <  1:2838668/150‑1 (MQ=255)

                                        atttcaggcgttgggtgtggatcactattgcaccgttcgtgacaGGTGTGTGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCggg                                                         <  1:167742‑M2/106‑1 (MQ=255)

                                        agaaagaGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCggg                                                         >  1:1296950/1‑150 (MQ=25)

                                         tttcaggcgttgggtgtggatcactattgcaccgttcgtgacaGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCgggg                                                        <  1:305204‑M2/107‑1 (MQ=255)

                                         gaaagaGATATTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCgggg                                                        <  1:1747809/150‑1 (MQ=21)

                                         gaaagaGATACAGCGCGTATACGATGAAAATCACAAGGTATACGGTGTGCGTAAAGTCTGGCGTCAGTTGTTACGGGAAGGTATCAGAGTGGCCAGATGCACTGTGGCACGTCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCgggg                                                        <  1:2506003/150‑1 (MQ=255)

                                          aaagaGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGt                                                       <  1:1844027/150‑1 (MQ=25)

                                           aagaGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTa                                                      >  1:2145993/1‑150 (MQ=25)

                                           aagaGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTa                                                      >  1:2268585/1‑150 (MQ=25)

                                             gagaTACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACGGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTaaa                                                    <  1:3531485/150‑1 (MQ=21)

                                             gagaTACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTaaa                                                    >  1:3622906/1‑150 (MQ=21)

                                              agaTACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGGAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTaaaa                                                   <  1:2586337/150‑1 (MQ=21)

                                              agaTACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTaaaa                                                   >  1:1138664/1‑150 (MQ=21)

                                               gaTACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGTGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTaaaaa                                                  <  1:18208/150‑1 (MQ=21)

                                               gaTACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGt                                                       >  1:2893006/1‑145 (MQ=21)

                                               gaTACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTaaaaa                                                  <  1:2770551/150‑1 (MQ=21)

                                               gaTACAGCGCGTATACGATGAAAATCACAAGGTATAAGGTGTGCGTAAAGTCTGGCGTCAGTTGTTACGGGAAGGTATCAGAGTGGCCAGATGCACTGTGGCACGTCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTaaaaa                                                  <  1:3832211/150‑1 (MQ=255)

                                                aTACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAg                                                 <  1:1352346/150‑1 (MQ=21)

                                                aTACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAg                                                 <  1:3000096/150‑1 (MQ=21)

                                                 tactgcgcGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAgg                                                >  1:1720641/5‑150 (MQ=21)

                                                  actgcgcGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCg                                                                                     >  1:3413813/4‑112 (MQ=255)

                                                   ctgcgcGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGGTGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTc                                              >  1:44679/3‑150 (MQ=21)

                                                   ctgcgcGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTc                                              <  1:1643186/148‑1 (MQ=21)

                                                   ctgcgcGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTc                                              >  1:2974288/3‑150 (MQ=21)

                                                   ctgcgcGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTc                                              <  1:882918/148‑1 (MQ=21)

                                                   ctgcgcGTATACGAAGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTc                                              <  1:1588700/148‑1 (MQ=21)

                                                    tgcgcGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTcc                                             <  1:1435692/149‑1 (MQ=21)

                                                     gcgcGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCg                                            <  1:2035865/150‑1 (MQ=21)

                                                      gtgtggatcactattgcaccgttcgtgacaGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGt                                           >  1:393549‑M2/31‑150 (MQ=255)

                                                      cgcgTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGt                                           >  1:491124/1‑150 (MQ=21)

                                                       gcgTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTa                                          >  1:790768/1‑150 (MQ=21)

                                                              cgatgggaatcatcaGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCAt                                   >  1:2699649/15‑150 (MQ=18)

                                                              cGATGAAAATCACAAGGTATACGGTGTGCGTAAAGTCTGGCGTCAGTTGTTACGGGAAGGTATCAGAGTGGCCAGATGCACTGTGGCACGTCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCAt                                   >  1:1532960/1‑150 (MQ=255)

                                                               gatgggaatcatcaGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATc                                  >  1:3359577/14‑150 (MQ=18)

                                                                   ggaatcatcaGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGTGGTAAAAAGGTCCGTACGACCATCAGcc                              >  1:2515763/10‑150 (MQ=18)

                                                                   ggaatcatcaGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGcc                              >  1:2912551/10‑150 (MQ=18)

                                                                     aatcatcaGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCgg                            <  1:718821/143‑1 (MQ=18)

                                                                      caccgttcgtgacaGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGa                           >  1:3771051‑M2/15‑150 (MQ=255)

                                                                       tcatcaGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGaa                          >  1:1481847/6‑150 (MQ=18)

                                                                        catcaGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCggg                                                         >  1:3544545/5‑118 (MQ=25)

                                                                        catcaGGTATACGGTGTGCGGAAAGTTTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCAGGTGTTCTCCGGGGGAAAAAGGTCCGTACGACCATCAGCCGGaaa                         <  1:3690268/146‑1 (MQ=18)

                                                                         atcaGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTTCAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAg                        <  1:1212482/147‑1 (MQ=18)

                                                                         atcaGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAg                        >  1:3299729/4‑150 (MQ=18)

                                                                          tcaGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGc                       >  1:3241155/3‑150 (MQ=18)

                                                                          caAGGTATACGGTGTGCGTAAAGTCTGGCGTCAGTTGTTACGGGAAGGTATCAGAGTGGCCAGATGCACTGTGGCACGTCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGc                       <  1:1651389/150‑1 (MQ=255)

                                                                           caGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGcc                      <  1:816860/149‑1 (MQ=18)

                                                                           aaGGTATACGGTGTGCGTAAAGTCTGGCGTCAGTTGTTACGGGAAGGTATCAGAGTGGCCAGATGCACTGTGGCACGTCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGcc                      <  1:2454040/150‑1 (MQ=255)

                                                                            aGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGNCAGATGTACAGTGNCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTANAAAGGTCCGTACGACCATCAGCCGGAAAGCCg                     <  1:3572245/150‑1 (MQ=18)

                                                                            aGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCg                     >  1:1782398/1‑150 (MQ=18)

                                                                            aGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCg                     >  1:1181070/1‑150 (MQ=18)

                                                                             ggTATACGGTGTGCGTAAAGTCTGGCGTCAGTTGTTACGGGAAGGTATCAGAGTGGCCAGATGCACTGTGGCACGTCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGTCGGAAAGCCGt                    >  1:2231912/1‑150 (MQ=255)

                                                                             ggTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGt                    >  1:1706525/1‑150 (MQ=18)

                                                                             ggTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATATACAGTGGCGCGCCTCATGGCGGTTCTGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGt                    >  1:1376356/1‑150 (MQ=18)

                                                                              gTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGtt                   >  1:649635/1‑150 (MQ=18)

                                                                               tataCGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTg                  <  1:3148645/150‑1 (MQ=18)

                                                                                     gtgtGCGTAAAGTCTGGCGTCAGTTGTTACGGGAAGGTATCAGAGTGGCCAGATGCACTGTGGCACGTCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcag            >  1:33999/1‑145 (MQ=255)

                                                                                     gtgtGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcag            >  1:2715175/1‑145 (MQ=17)

                                                                                       gtgCGTAAAGTCTGGCGTCAGTTGTTCCGGGAAGGTATCAGAGTGGCCAGATGCACTGTGGCATGTCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATc                                  >  1:3011089/1‑126 (MQ=255)

                                                                                         gCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcaggcga        >  1:2315192/1‑141 (MQ=17)

                                                                                         gCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcaggcga        <  1:73512/150‑10 (MQ=17)

                                                                                          cGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcaggcgac       <  1:59784/150‑11 (MQ=14)

                                                                                          cGTAAAGTCTGGAGTCAGTTGTGACGGGAAGGAATCAGGGTAGCCAGGGGTACAGTGGCGCGCCCCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcaggcgac       <  1:3443127/150‑11 (MQ=2)

                                                                                          cGTAAAGTCTGGAGTCAGTTATTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCGTCAGCCGGAAAGCCGTTGccgcaggcgac       <  1:2000805/150‑11 (MQ=14)

                                                                                           gTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcaggcgacc      >  1:1031716/1‑139 (MQ=14)

                                                                                             aaaGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcaggcgaccgc    >  1:684476/1‑137 (MQ=14)

                                                                                              aaGTCTTGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcaggcgaccgcg   <  1:3202884/150‑15 (MQ=11)

                                                                                               aGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCAGTTGccgcaggcgaccgcgt  >  1:699221/1‑135 (MQ=11)

                                                                                                gTCTGGAGTCAGTTGTTACGGGAAGGACTCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGACAGCCGTTGccgcaggcg         >  1:3141918/1‑134 (MQ=14)

                                                                                                           gttgttACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCAtta                                 <  1:1090376/107‑3 (MQ=17)

GATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACAGCGCGTATACGATGAAAATCACAAGGTATACGGTGTGCGTAAAGTCTGGCGTCAGTTGTTACGGGAAGGTATCAGAGTGGCCAGATGCACTGTGGCACGTCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  Xen14_202/192‑421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`