BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	Xen14_143	1,030	A→C	intergenic (‑724/–)	Xen14_04759 ← / –	hypothetical protein/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Xen14_143	1,030	0	A	C	89.5%	1860.5 / 167.0	713	intergenic (‑724/–)	Xen14_04759/–	hypothetical protein/–
Reads supporting (aligned to +/- strand): ref base A (46/29); new base C (341/295); total (387/326)
Fisher's exact test for biased strand distribution p-value = 2.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

Only 100 of 724 total aligned reads displayed.

GAACCGGCAGGGGAAACACCCGGAGCAACACCTCCGCCCCTTCCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTATGACAGGCTGTTCAGTGCAGAACGTGAAGGTGGTGCGCTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGAGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTGAACTGTAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  Xen14_143/882‑1107

   ccGGCAGGGGAAACACCTGGAGCAACACCTTCGCCCCTTCCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGaaa                                                                                          <  1:2853813/150‑1 (MQ=255)

   ccGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTTCCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGaaa                                                                                          <  1:2700979/150‑1 (MQ=255)

   ccGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTTCCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGaaa                                                                                          <  1:2109396/150‑1 (MQ=255)

    cGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTTCCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGaaaa                                                                                         <  1:2990134/150‑1 (MQ=255)

    cGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTTCCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGaaaa                                                                                         >  1:3261917/1‑150 (MQ=255)

     ggCAGGGGAAACACCCGGAGCAACACCTCCGCCCCTTCCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTATGACAGGCTGTTCAGTGCAGAACGTGAAGGTGGTGCGCTGACAGAAGTTGCGTGCTGGGCCCATGCCCGgag                                                                                             >  1:2235073/1‑145 (MQ=255)

      gCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTTCCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑t                                                                                      >  1:3637002/1‑150 (MQ=255)

      gCAGGGGAAACACCCGGAGCAACACCTCCGTCCCTTCCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑t                                                                                      <  1:2167772/150‑1 (MQ=255)

       cAGGGGATACACCCGGAGCAACACCTTCGCCCCTTCCGGGGTATTCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑Tc                                                                                     <  1:776561/150‑1 (MQ=255)

        aGGGGAAACACCCGGAGCAACACCTTCGCCCCTTCCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑Tcc                                                                                    <  1:3394158/150‑1 (MQ=255)

        aGGGGAAACACCCGGAGCAACACCTCCGTCCCTTCCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑Tcc                                                                                    >  1:143253/1‑150 (MQ=255)

         ggggAAACACCCGGAGCAACACCTTCGCCCCTTCCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCa                                                                                   >  1:3295437/1‑150 (MQ=255)

          gggAAACACCCGGAGCAACACCTTCGCCCCTTCCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCAc                                                                                  <  1:2628555/150‑1 (MQ=255)

          gggAAACACCCGGAGCAACACCTTCGCCCCTTCCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCAc                                                                                  >  1:2069372/1‑150 (MQ=255)

                cacCCGGAGCAACACCTTCGCCCCTTCCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGta                                                                            >  1:1454679/1‑150 (MQ=255)

                cacCCGGAGCAACACCTTCGCCCCTTCCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGta                                                                            <  1:2817244/150‑1 (MQ=255)

                 acCCGGAGCAACACCTTCGCCCCTTCCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGtat                                                                           <  1:2669204/150‑1 (MQ=255)

                  cccGGAGCAACACCTCCGCCCCTTCCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTATGACAGGCTGTTCAGTGCAGAACGTGAAGGTGGTGCGCTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGAGAAAAAATCAACGATGtat                                                                           >  1:460567/1‑150 (MQ=255)

                    cGGAGCAACACCTTCGCCCCTTCCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACa                                                                        <  1:896087/150‑1 (MQ=255)

                    cGGAGCAACACCTTCGCCCCTTCCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACa                                                                        <  1:1959320/150‑1 (MQ=255)

                       aGCAACACCTTCGCCCCTTCCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATca                                                                     >  1:294333/1‑150 (MQ=255)

                        gCAACACCTTCGCCCCTTCCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATcag                                                                    >  1:1494641/1‑150 (MQ=255)

                        gCAACACCTTCGCCCCTTCCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATcag                                                                    <  1:2691503/150‑1 (MQ=255)

                        gCAACACCTTCGCCCCTTCCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTCGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATcag                                                                    >  1:2191879/1‑150 (MQ=255)

                         cAACACCTCCGTCCCTTCCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACAACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATcagc                                                                   >  1:1599023/1‑150 (MQ=255)

                          aaCACCTTCGCCCCTTCCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATcagca                                                                  >  1:1164505/1‑150 (MQ=255)

                          aaCACCTTCGCCCCTTCCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATcagca                                                                  >  1:3675099/1‑150 (MQ=255)

                           acacCTTCGCCCCTTCCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGACGAAAAA‑TCCACGATGTATACATcagcag                                                                 <  1:1288615/150‑1 (MQ=255)

                             acCTTCGCCCCTTCCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAgcagca                                                               >  1:3483761/1‑150 (MQ=255)

                             acCTCCGCCCCTTCCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTATGACAGGCTGTTCAGTGCAGAACGTGAAGGTGGTGCGCTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGAGAAAAA‑TCCACGATGTATACATCAgcagca                                                               <  1:3720055/150‑1 (MQ=255)

                                   gCCCCTTCCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTg                                                         >  1:1407759/1‑150 (MQ=255)

                                    tcccTTCCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGc                                                        <  1:2895792/149‑1 (MQ=255)

                                    ccccTTCCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGc                                                        >  1:1660022/1‑150 (MQ=255)

                                     cccTTCCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGATAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCg                                                       <  1:523901/150‑1 (MQ=255)

                                     cccTTCCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCg                                                       <  1:3349481/150‑1 (MQ=255)

                                     cccTTCCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCg                                                       >  1:2376226/1‑150 (MQ=255)

                                     cccTTCCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCg                                                       <  1:2527950/150‑1 (MQ=255)

                                       cTTCCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGAc                                                     >  1:2470691/1‑150 (MQ=255)

                                       cTTCCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACATGAAGGTGGTGCACTAACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCaaaa                                                            >  1:58073/1‑143 (MQ=255)

                                        ttCCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTATGACAGGCTGTTCAGTGCAGAATGTGAAGGTGGTGCGCTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGAGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACg                                                    >  1:1544428/1‑150 (MQ=255)

                                        ttCCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACg                                                    <  1:3763608/150‑1 (MQ=255)

                                          ccGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTATGACAGGCTGTTCAGTGCAGAACGTGAAGGTGGTGCGCTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGAGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGc                                                  >  1:3050342/1‑150 (MQ=255)

                                          ccGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGc                                                  <  1:1749133/150‑1 (MQ=255)

                                            ggggTATCCTGCAGGCGGATGCGTTCGCAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCag                                                <  1:1670172/150‑1 (MQ=255)

                                               gTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCagaag                                             >  1:2496104/1‑150 (MQ=255)

                                                tATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCagaaga                                            <  1:2275951/150‑1 (MQ=255)

                                                   ccTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGc                                         <  1:1824142/150‑1 (MQ=255)

                                                   ccTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGc                                         <  1:3162595/150‑1 (MQ=255)

                                                   ccTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGc                                         >  1:1895428/1‑150 (MQ=255)

                                                    cTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGcc                                        >  1:626941/1‑150 (MQ=255)

                                                    cTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGcc                                        >  1:2330774/1‑150 (MQ=255)

                                                    cTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGcc                                        <  1:766686/150‑1 (MQ=255)

                                                    cTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGcc                                        <  1:1481305/150‑1 (MQ=255)

                                                     tGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCCGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGccc                                       <  1:381034/150‑1 (MQ=255)

                                                          gCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCTCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGCCAGAAGAAGCCCTGAAg                                  <  1:2580588/150‑1 (MQ=255)

                                                           cGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGc                                 <  1:3788292/150‑1 (MQ=255)

                                                           cGGATGCGTTCACAGGCTACGACAGGTTGTTCAGTGCAGAACGTGAAGTTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGc                                 <  1:1005006/150‑1 (MQ=255)

                                                            ggATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCg                                <  1:1187730/150‑1 (MQ=255)

                                                            ggATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCg                                <  1:1714329/150‑1 (MQ=255)

                                                              aTGCGTTCACAGGTTATGACAGGCTGTTCAGTGCAGAACGTGAAGGTGGTGCGCTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGAGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGaa                              <  1:245539/150‑1 (MQ=255)

                                                              aTGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGaa                              >  1:2026975/1‑150 (MQ=255)

                                                                 cGTTCACAGGTTATGACAGGCTGTTCAGTGCAGAACGTGAAGGTGGTGCGCTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGAGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCa                           <  1:1633775/150‑1 (MQ=255)

                                                                 cGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCa                           >  1:837467/1‑150 (MQ=255)

                                                                  gTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAg                          >  1:1221535/1‑150 (MQ=255)

                                                                    tCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGc                                 >  1:1272/1‑141 (MQ=255)

                                                                    tCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTg                        >  1:1092677/1‑150 (MQ=255)

                                                                    tCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTg                        >  1:924711/1‑150 (MQ=255)

                                                                    tCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTg                        <  1:3092523/150‑1 (MQ=255)

                                                                    tCACAGGTTACGACAGGTTGATCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGcccgagcaaaacccgcag                        >  1:2415025/1‑135 (MQ=255)

                                                                     cacaGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTGa                       <  1:236841/150‑1 (MQ=255)

                                                                     cacaGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTGa                       <  1:2225682/150‑1 (MQ=255)

                                                                      acaGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTGaa                      >  1:230741/1‑150 (MQ=255)

                                                                      acaGGTTACGACAGATTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTGaa                      >  1:1979898/1‑150 (MQ=255)

                                                                       caGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTGAAc                     <  1:3240894/150‑1 (MQ=255)

                                                                        aGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAGGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTGAACt                    <  1:1049131/150‑1 (MQ=255)

                                                                          gTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTGAACTGt                  >  1:3434902/1‑150 (MQ=255)

                                                                           ttacgACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGGGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTGAACTGTa                 >  1:1795138/5‑150 (MQ=255)

                                                                            tacgACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTGAACTGTAc                <  1:3533562/147‑1 (MQ=255)

                                                                             acgACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGaa                              >  1:664692/3‑135 (MQ=255)

                                                                             acgACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTGAACTGTAcg               >  1:422917/3‑149 (MQ=255)

                                                                             acgACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTGAACTGTAcg               >  1:2835212/3‑149 (MQ=255)

                                                                              cgACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGCCGGCAGAAGAAGCCCTGAAGCGAATCAGTGAACTGTAcgc              >  1:1358494/2‑148 (MQ=255)

                                                                              cgACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTGAACTGTAcgc              >  1:3332048/2‑148 (MQ=255)

                                                                               gACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTGAACTGTAcgcc             <  1:401576/150‑4 (MQ=255)

                                                                                 cAGGCTGTTCAGTGCAGAACGTGAAGGTGGTGCGCTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGAGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTGaa                      >  1:3120451/1‑139 (MQ=255)

                                                                                    gttGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCGGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTGAACTGTAcgccatcga        >  1:1024322/3‑142 (MQ=255)

                                                                                    gttGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTGAACTGTAcgccatcga        >  1:723280/3‑142 (MQ=255)

                                                                                       gTTCAGTGCAGAACGTGAAGGTGGTGCGCTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGAGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTGAACTGTAcgccatcgagga     >  1:1290567/1‑139 (MQ=255)

                                                                                       gTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTGAACTGTAcgccatcgagga     >  1:3284210/1‑139 (MQ=255)

                                                                                       gTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTGAACTGTAcgccatcgagga     >  1:3185093/1‑139 (MQ=255)

                                                                                        ttCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGGAGCCCTGAAGCGAATCAGTGAACTGTAcgccatcgaggat    >  1:27432/1‑138 (MQ=255)

                                                                                        ttCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTGAACTGTAcgccatcgaggat    >  1:361448/1‑138 (MQ=255)

                                                                                         tCAGTGCAGAACGTGAAGGTGGTGCGCTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGAGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTGAACTGTAcgccatcgaggatg   >  1:813939/1‑137 (MQ=255)

                                                                                         tCAGTGCAGAACGTGAAGGTGGTGCGCTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGAGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTGAACTGTAcgccatcgaggatg   >  1:1925569/1‑137 (MQ=255)

                                                                                         tCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCt                                      <  1:3208128/115‑1 (MQ=255)

                                                                                         tCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTGAACTGTAcgccatcgaggatg   >  1:2970683/1‑137 (MQ=255)

                                                                                         tCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTGAACTGTAcgccatcgaggatg   >  1:2463616/1‑137 (MQ=255)

                                                                                          cAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTGAACTGTAcgccatcgaggatga  <  1:1145118/150‑15 (MQ=255)

                                                                                          cAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTGAACTGTAcgccatcgaggatga  <  1:977731/150‑15 (MQ=255)

                                                                                                                gcacTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCGGAAGAAGCCCTGAAGCGAATCAGTGAACTGTAcgc              >  1:488218/4‑114 (MQ=255)

GAACCGGCAGGGGAAACACCCGGAGCAACACCTCCGCCCCTTCCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTATGACAGGCTGTTCAGTGCAGAACGTGAAGGTGGTGCGCTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGAGAAAAA‑TCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTGAACTGTAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  Xen14_143/882‑1107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`