BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	Xen14_143	967	C→T	intergenic (‑661/–)	Xen14_04759 ← / –	hypothetical protein/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Xen14_143	967	0	C	T	89.9%	2611.3 / 164.1	1123	intergenic (‑661/–)	Xen14_04759/–	hypothetical protein/–
Reads supporting (aligned to +/- strand): ref base C (74/39); new base T (553/455); total (627/496)
Fisher's exact test for biased strand distribution p-value = 3.56e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1130 total aligned reads displayed.

GTCCGGGATGATCGCAATGCGGGTTCGTCATCTCCTCCGGCGGTCTGGTTCGCGTACTCACCGAACCGGCAGGGGAAACACCCGGAGCAACACCTCCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTATGACAGGCTGTTCAGTGCAGAACGTGAAGGTGGTGCGCTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGAGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTGAACTGTAC‑‑‑‑  >  Xen14_143/820‑1107

gTCCGGGATGATCGCAATGCGGGTTCGTCATCTCCTCCGGCGGTCTGGTTCGCGTACTCACCGAACCGGCAGGGGAAACACCCGGAGCAACACCTCCGCCTCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTATGACAGGCTg                                                                                                                                                >  1:3202826/1‑150 (MQ=255)

  ccGGGATGATCACAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGgttgtt                                                                                                                                              >  1:950978/1‑150 (MQ=255)

   cGGGATGATCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTc                                                                                                                                             >  1:340471/1‑150 (MQ=255)

     ggATGATCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAg                                                                                                                                           <  1:2751239/150‑1 (MQ=255)

     ggATGATCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTT‑CCGGGGTATCCTGCAGGCGAATGCGTTCACAGGTTACGACAGGTTGTTCAg                                                                                                                                           >  1:1364479/1‑150 (MQ=255)

      gatgatCGCAATGCGGGTTCGTCATCTCCTCCGGCGGTCTGGTTCGCGTACTCACCGAACCGGCAGGGGAAACACCCGGAGCAACACCTCCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTCCACAGGTTATGACAGGCTGTTCAGt                                                                                                                                          <  1:1212187/150‑1 (MQ=255)

          atCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAg                                                                                                                                      >  1:2036446/1‑150 (MQ=255)

           tCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTTGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCATT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACCGGTTACGACAGGTTGTTCAGTGCAGa                                                                                                                                     >  1:3827239/1‑150 (MQ=255)

           tCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGa                                                                                                                                     <  1:2261483/150‑1 (MQ=255)

             gCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTCGCAGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAAc                                                                                                                                   <  1:2497543/150‑1 (MQ=255)

              cAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACg                                                                                                                                  >  1:2224851/1‑150 (MQ=255)

                 tgtgggTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTTTCCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTg                                                                                                                                >  1:756877/4‑150 (MQ=255)

                    gggTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAgg                                                                                                                            >  1:1969104/1‑150 (MQ=255)

                          gTCATCTCCTCCAGCGGTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTgc                                                                                                                      >  1:2341916/1‑150 (MQ=255)

                          gTCATCTCCTCCAGCGGTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTT‑CCGGGGTATCCTGCAGGCGAATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTgc                                                                                                                      <  1:2950402/150‑1 (MQ=255)

                              tctcCTCCGGCGGTCTGGTTAGCGTACTCACCGAACCGGCAGGGGAAAAACCCGGAGCAACACCTCCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGGCCACAGGTTATGACAGGCTGTTCAGTGCAGAACGTGAAGGTGGTGCGCTg                                                                                                                  <  1:1386464/150‑1 (MQ=255)

                                tcctccAGCGGTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGAc                                                                                                                >  1:3468050/1‑150 (MQ=255)

                                 cctccAGCGGTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACa                                                                                                               >  1:3133739/1‑150 (MQ=255)

                                  ctccAGCGGTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAg                                                                                                              >  1:557862/1‑150 (MQ=255)

                                     cagcggTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAg                                                                                                           <  1:2596635/148‑1 (MQ=255)

                                     cagcggTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAg                                                                                                           <  1:885682/148‑1 (MQ=255)

                                       gcggTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTCCGTCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGtt                                                                                                         <  1:2441377/150‑1 (MQ=255)

                                         ggTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGc                                                                                                       >  1:2082390/1‑150 (MQ=255)

                                              ggTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCt                                                                                                  >  1:171167/1‑150 (MQ=255)

                                                     gTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTTACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCa                                                                                           <  1:3249712/150‑1 (MQ=255)

                                                     gTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAAGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCa                                                                                           <  1:286085/150‑1 (MQ=255)

                                                     gTACTCACCGAACCGGCAGGGGAAACACCCGGAGCAACACCTCCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTATGACAGGCTGTTCAGTGCAGAACGTGAAGGTGGTGCGCTGACAGAAGTTGCGTGCTGGGCCCa                                                                                           <  1:1016623/150‑1 (MQ=255)

                                                       aCTCACCGAACCGGCAGGGGAAACACCCGGAGCAACACCTCCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTATGACAGGCTGTTCAGTGCAGAACGTGAAGGTGGTGCGCTGACAGAAGTTGCGTGCTGGGCCCATg                                                                                         <  1:680371/150‑1 (MQ=255)

                                                        ctcgccGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGc                                                                                        >  1:3037796/5‑150 (MQ=255)

                                                        ctcgccGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGc                                                                                        <  1:3101155/146‑1 (MQ=255)

                                                        ctcgccGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGc                                                                                        >  1:640902/5‑150 (MQ=255)

                                                        ctcgccGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAAGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGc                                                                                        >  1:3554542/5‑150 (MQ=255)

                                                         tcgccGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACCGAAGTTGCGTGCTGGGCCCATGcc                                                                                       <  1:2107122/147‑1 (MQ=255)

                                                         tcgccGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGcc                                                                                       <  1:2398726/147‑1 (MQ=255)

                                                          cgccGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGAGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGccc                                                                                      >  1:1620273/3‑150 (MQ=255)

                                                             cGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCggc                                                                                   <  1:3223787/150‑2 (MQ=255)

                                                              gAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCggcg                                                                                  >  1:3375374/1‑148 (MQ=255)

                                                              gAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCggcg                                                                                  <  1:3772184/150‑3 (MQ=255)

                                                                aCCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGaa                                                                                >  1:2680154/1‑150 (MQ=255)

                                                                 ccGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGaaa                                                                               <  1:2700979/150‑1 (MQ=255)

                                                                   ggCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTgcact                                                                                                                   <  1:1251737/112‑4 (MQ=255)

                                                                   ggCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCggcg                                                                                  >  1:811712/1‑143 (MQ=255)

                                                                   ggCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCggcg                                                                                  >  1:2164651/1‑143 (MQ=255)

                                                                   ggCAGGGGAAACACCCGGAGCAACACCTCCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTATGACAGGCTGTTCAGTGCAGAACGTGAAGGTGGTGCGCTGACAGAAGTTGCGTGCTGGGCCCATGCCCGgag                                                                                  >  1:2235070/1‑145 (MQ=255)

                                                                   ggCAGGGGAAACACCCGGAGCAACACCTCCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTATGACAGGCTGTTCAGTGCAGAACGTGAAGGTGGTGCGCTGACAGAAGTTGCGTGCTGGGCCCATGCCCGgag                                                                                  >  1:503885/1‑145 (MQ=255)

                                                                    gCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAAt                                                                            >  1:2983600/1‑150 (MQ=255)

                                                                     cAGGGGATACACCCGGAGCAACACCTTCGCCCCTT‑CCGGGGTATTCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATc                                                                           <  1:776561/150‑1 (MQ=255)

                                                                      aGGGGAAACACCCGGAGCAACACCTTCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGTTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAATTTGCGTGCTGGGCCCATGCCCGGCGAAAAATcc                                                                          <  1:3740781/150‑1 (MQ=255)

                                                                      aGGGGAAACACCCGGAGCAACACCTTCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATcc                                                                          >  1:2906998/1‑150 (MQ=255)

                                                                      aGGGGAAACACCCGGAGCAACACCTCCGTCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATcc                                                                          >  1:143249/1‑150 (MQ=255)

                                                                         ggAAACACCCGGAGCAACACCTCCGTCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCATTGACGGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACg                                                                       <  1:2369671/150‑1 (MQ=255)

                                                                              cacCCGGAGCAACACCTTCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGta                                                                  <  1:2817244/150‑1 (MQ=255)

                                                                               acCCGGAGCAACACCTTCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCGGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGtat                                                                 >  1:2014091/1‑150 (MQ=255)

                                                                                cccGGAGCAACACCTTCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGtata                                                                >  1:1663365/1‑150 (MQ=255)

                                                                                cccGGAGCAACACCTTCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGtata                                                                >  1:1724380/1‑150 (MQ=255)

                                                                                 ccGGAGCAACACCTCCGTCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCCATGCAGAACGTGAAGGTGGTGCACTGAAAGAAGTTGCGTGCTGGGCCCACGCCCGGCGAAAAACCCACGATGTATAc                                                               >  1:3617001/1‑150 (MQ=255)

                                                                                  cGGAGCAACACCTTCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACa                                                              <  1:427603/150‑1 (MQ=255)

                                                                                    gAGCAACACCTTCGCCCCTT‑CCGGGGTGTCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGCGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATc                                                            <  1:710531/150‑1 (MQ=255)

                                                                                     aGCAACACCTTCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAAGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATca                                                           >  1:916196/1‑150 (MQ=255)

                                                                                      gCAACACCTTCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATcag                                                          >  1:1494645/1‑150 (MQ=255)

                                                                                      gCAACACCTTCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTCGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATcag                                                          >  1:2191879/1‑150 (MQ=255)

                                                                                              ttcGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCaaaa                                                  <  1:1280294/148‑1 (MQ=255)

                                                                                                 gCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCggc                                                                                   <  1:158017/114‑2 (MQ=255)

                                                                                                 gCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACg                                                                       <  1:1648976/126‑1 (MQ=255)

                                                                                                 gCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTNCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTg                                               <  1:1777543/150‑1 (MQ=255)

                                                                                                   cccTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCATTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCg                                             <  1:262995/150‑1 (MQ=255)

                                                                                                   cccTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCg                                             >  1:2654253/1‑150 (MQ=255)

                                                                                                     cTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAGCGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGAc                                           >  1:3521474/1‑150 (MQ=255)

                                                                                                     cTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGAc                                           <  1:3176682/150‑1 (MQ=255)

                                                                                                      tt‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTATGACAGGCTGTTCAGTGCAGAATGTGAAGGTGGTGCGCTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGAGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACg                                          >  1:1544428/1‑150 (MQ=255)

                                                                                                      tt‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACg                                          <  1:1467312/150‑1 (MQ=255)

                                                                                                         ccGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGc                                        <  1:1749133/150‑1 (MQ=255)

                                                                                                            gggTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCaga                                     <  1:1090965/150‑1 (MQ=255)

                                                                                                            gggTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTAAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCaga                                     <  1:1811846/150‑1 (MQ=255)

                                                                                                              gTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCagaag                                   <  1:2324070/150‑1 (MQ=255)

                                                                                                               tATCCTGCAGGCGGATGCATTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCagaaga                                  <  1:841974/150‑1 (MQ=255)

                                                                                                                  ccTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGc                               >  1:580842/1‑150 (MQ=255)

                                                                                                                  ccTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGc                               >  1:1895428/1‑150 (MQ=255)

                                                                                                                  ccTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAA‑GTGCGACGGCAGAAGAAGcc                              <  1:3687029/150‑1 (MQ=255)

                                                                                                                   cTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGcc                              <  1:1481309/150‑1 (MQ=255)

                                                                                                                    tGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATcag                                                          >  1:2471505/1‑121 (MQ=255)

                                                                                                                     gCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAA‑TCCACGATGTATATATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTg                           <  1:2297501/150‑1 (MQ=255)

                                                                                                                       aGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGa                          <  1:1032357/150‑1 (MQ=255)

                                                                                                                        ggCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGaa                         <  1:1141638/150‑1 (MQ=255)

                                                                                                                          cGGATGCGTTCACAGGCTACGACAGGTTGTTCAGTGCAGAACGTGAAGTTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGc                       <  1:1005006/150‑1 (MQ=255)

                                                                                                                             aTGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGaa                    <  1:408072/150‑1 (MQ=255)

                                                                                                                               gCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATc                  <  1:344226/150‑1 (MQ=255)

                                                                                                                               gCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATc                  <  1:2554749/150‑1 (MQ=255)

                                                                                                                                cattCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGc                               <  1:3660046/134‑1 (MQ=255)

                                                                                                                                 gTTCACAGGTTACGAAAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAg                >  1:3298238/1‑150 (MQ=255)

                                                                                                                                   tCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTg              >  1:463647/1‑150 (MQ=255)

                                                                                                                                   tCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTg              <  1:1166729/150‑1 (MQ=255)

                                                                                                                                    cacaGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTGa             <  1:3281884/150‑1 (MQ=255)

                                                                                                                                          ttacgACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTGAACTGTa       >  1:1937779/5‑150 (MQ=255)

                                                                                                                                            acgACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTGAACTGTAcg     >  1:2835215/3‑149 (MQ=255)

                                                                                                                                            acgACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTGAACTGTAcg     >  1:1846279/3‑149 (MQ=255)

                                                                                                                                             cgACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTGAACTGTAcgc    >  1:3350419/2‑148 (MQ=255)

                                                                                                                                             cgACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTGAACTGTAcgc    >  1:3332048/2‑148 (MQ=255)

                                                                                                                                             cgACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTGAACTGTAcgc    >  1:1312331/2‑148 (MQ=255)

                                                                                                                                               aCAGGCTGTTCAGTGCAGAACGTGAAGGTGGTGCGCTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGAGAAAAATCCACGATGTACACATCAGCAGCAAAAGTGCGACGGCAGAAGCAGCCCTGAAGCGAATCAGTGAACTGTAcgcca  >  1:3793289/1‑146 (MQ=255)

GTCCGGGATGATCGCAATGCGGGTTCGTCATCTCCTCCGGCGGTCTGGTTCGCGTACTCACCGAACCGGCAGGGGAAACACCCGGAGCAACACCTCCGCCCCTT‑CCGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTATGACAGGCTGTTCAGTGCAGAACGTGAAGGTGGTGCGCTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGAGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTGAACTGTAC‑‑‑‑  >  Xen14_143/820‑1107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`