BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	Xen14_143	753	C→T	intergenic (‑447/–)	Xen14_04759 ← / –	hypothetical protein/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Xen14_143	753	0	C	T	88.8%	2426.1 / 186.7	1086	intergenic (‑447/–)	Xen14_04759/–	hypothetical protein/–
Reads supporting (aligned to +/- strand): ref base C (64/57); new base T (500/461); total (564/522)
Fisher's exact test for biased strand distribution p-value = 9.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01

Only 100 of 1100 total aligned reads displayed.

CGTCAGAGTGAAATCTTTGCCCGACAGGGTGTCGAACTGAGCCGTGCATTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCC‑GCTGAATGATGCCCTGTACCGTTATGTGATGAATACCCGCAAGCTTCACACTGACGACACACCGGTAAAGGTACTGGCACCGGACCTGAAAAAGACGAAAACAGGGCGCATCTGGACGTATGTCCGGGATGATCGCAATGCGGGTTCGTCATCTCCTCCGGCGGTCTGGTTCGCGTACTCACCGAACCGGCAGGGGAAACA  >  Xen14_143/607‑899

 gTCAGAGTGAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGaca                                                                                                                                                >  1:1691182/1‑150 (MQ=255)

       gTGAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTGATGAATACCCGCAAGGTTCACACTGATGACACACCgg                                                                                                                                          >  1:1394327/1‑150 (MQ=255)

       gTGAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCgg                                                                                                                                          >  1:3438156/1‑150 (MQ=255)

         gAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTa                                                                                                                                        >  1:1060605/1‑150 (MQ=255)

         gAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTa                                                                                                                                        <  1:1987853/150‑1 (MQ=255)

          aaaTCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAACACCCGCAAGGTTCACACTGATGACACACCGGTaa                                                                                                                                       >  1:839848/1‑150 (MQ=255)

           aaTCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTaaa                                                                                                                                      <  1:3151812/150‑1 (MQ=255)

           aaTCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTaaa                                                                                                                                      >  1:3562596/1‑150 (MQ=255)

           aaTCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTaaa                                                                                                                                      >  1:2742284/1‑150 (MQ=255)

           aaTCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGCACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTaaa                                                                                                                                      >  1:3169125/1‑150 (MQ=255)

            aTCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAg                                                                                                                                     <  1:1445656/150‑1 (MQ=255)

            aTCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAACACCCGCAAGGTTCACACTGATGACACACCGGTAAAg                                                                                                                                     >  1:1969467/1‑150 (MQ=255)

             tCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAgg                                                                                                                                    >  1:2634521/1‑150 (MQ=255)

             tCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAgg                                                                                                                                    >  1:468964/1‑150 (MQ=255)

              cTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAACACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGt                                                                                                                                   >  1:596566/1‑150 (MQ=255)

                  gCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAACACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTg                                                                                                                               >  1:2300071/1‑150 (MQ=255)

                    ccGACAGGGTGTCGAACTGAGCCGTGCATTACTTTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCC‑GCTGAATGATGCCCTGTACCGTTATGTGATGAATACCCGCAAGCTTCACACTGACGACACACCGGTAAAGGTACTGGc                                                                                                                             <  1:2119144/150‑1 (MQ=255)

                       ccAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCAcc                                                                                                                          <  1:93128/149‑1 (MQ=255)

                           ggTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCACGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCgggt                                                                                                                      <  1:2227675/150‑3 (MQ=255)

                           ggTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCgggt                                                                                                                      <  1:2919980/150‑3 (MQ=255)

                            gtgtCGAACTGAGCCGGGCCTTACTCTTCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCAGC‑GAATGACGCCCTGTACCGTTATGTAATGAACCCCCGCAAAGTTCACACTGATGGCACACCGGTAAAGGTACTGGCACCgggtc                                                                                                                     >  1:3685499/1‑147 (MQ=255)

                              gtCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCgggtcag                                                                                                                   >  1:2779683/1‑145 (MQ=255)

                              gtCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCgggtcag                                                                                                                   <  1:2851780/150‑6 (MQ=255)

                               tCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCgggtcaga                                                                                                                  <  1:3462213/150‑7 (MQ=255)

                                  aaCTGAGCCGTGCATTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCC‑GCTGAATGATGCCCTGTACCGTTATGTGATGAATACCCGCAAGCTTCACACTGACGACACACCGGTAAAGGTACTGGCACCGGACCTGaaaa                                                                                                               >  1:653987/1‑150 (MQ=255)

                                   aCTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGNACTGGCACCgggtcagaaaaa                                                                                                              <  1:3813401/150‑11 (MQ=255)

                                    cTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCgggtcagaaaaag                                                                                                             <  1:1593088/150‑12 (MQ=255)

                                    cTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCgggtcagaaaaag                                                                                                             <  1:2534104/150‑12 (MQ=255)

                                    cTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCgggtcagaaaaag                                                                                                             <  1:884916/150‑12 (MQ=255)

                                    cTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACACGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCgggtcagaaaaag                                                                                                             <  1:1482974/150‑12 (MQ=255)

                                    cTGAGCCGGGCCTTACTCTCCAACTAGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCgggtcagaaaaag                                                                                                             <  1:2056379/150‑12 (MQ=255)

                                      gAGCCGTGCATTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCC‑GCTGAATGATGCCCTGTACCGTTATGTGATGAATACCCGCAAGCTTCACACTGACGACACACCGGTAAAGGTACTGGCACCGGACCCGAAAAAGAc                                                                                                           <  1:2513791/150‑1 (MQ=255)

                                      gAGCCGGGCCTTACTCTCCAACTGGGTTTACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCgggtcagaaaaaggc                                                                                                           <  1:3238040/150‑14 (MQ=255)

                                      gAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCgggtcagaaaaaggc                                                                                                           <  1:2440263/150‑14 (MQ=255)

                                      gAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAACACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCgggtcagaaaaaggc                                                                                                           <  1:2468068/150‑14 (MQ=255)

                                          cgggccttACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACATACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGaaa                                                                                                       <  1:1784009/144‑1 (MQ=255)

                                                ttACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAggg                                                                                                 <  1:219972/150‑1 (MQ=255)

                                                ttACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAggg                                                                                                 <  1:1293085/150‑1 (MQ=255)

                                                ttACTCTCCAACTGGGTTGACGCATGCTGCCAGTTAATGACACC‑GGTGAATGATACCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAggg                                                                                                 <  1:3787128/150‑1 (MQ=255)

                                                 tACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACG‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGgc                                                                                                <  1:675408/150‑1 (MQ=255)

                                                   ctctCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGgcgt                                                                                              >  1:410796/1‑149 (MQ=255)

                                                   ctctCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATAAACACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGgcgt                                                                                              >  1:1730382/1‑149 (MQ=255)

                                                    tctcCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGgcgta                                                                                             >  1:1885244/1‑148 (MQ=255)

                                                          aCTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGa                                                                                       <  1:3120225/150‑1 (MQ=255)

                                                              ggTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTa                                                                                   <  1:2147335/150‑1 (MQ=255)

                                                              ggTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGATACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTa                                                                                   >  1:3023678/1‑150 (MQ=255)

                                                                ttGACGCGTGCTGCCAGTTAATGACAAC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGAAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTAAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATg                                                                                 >  1:3272114/1‑150 (MQ=255)

                                                                   aCGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTGCCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTcc                                                                              <  1:1201191/150‑1 (MQ=255)

                                                                    cgcgTGCTGCCAGTTAATGCCACC‑GGCGAATGATGCCCTGTACCGTTATGTACTGAATACCCGCAAGGTTCCCACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCg                                                                             >  1:260271/1‑150 (MQ=255)

                                                                    cgcgTGCTACCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCg                                                                             <  1:561758/150‑1 (MQ=255)

                                                                      cgTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGCACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCggg                                                                           >  1:529121/1‑150 (MQ=255)

                                                                          ctgcCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGATATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGgatga                                                                       >  1:114836/1‑150 (MQ=255)

                                                                           tgcCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGgatgat                                                                      <  1:2591290/150‑1 (MQ=255)

                                                                             ccAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTATTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCg                                                                    <  1:3607905/150‑1 (MQ=255)

                                                                              cAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGc                                                                   >  1:3641447/1‑150 (MQ=255)

                                                                               aGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCa                                                                  >  1:1117875/1‑150 (MQ=255)

                                                                               aGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACAGGTAAAGGTAATGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATc                                                                                           >  1:3523809/1‑125 (MQ=255)

                                                                                     tGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATgtgg                                                            >  1:1273845/1‑147 (MQ=255)

                                                                                           ccggtGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCg                                                       <  1:2321234/146‑1 (MQ=255)

                                                                                             ggtGAATGATGCCCTGTACCGTTATGTAATGAATACCAGCAAGGTTCACACTGATGACACCCCGGTAACGGTACTGGCACCGGGTCAGAACAAGGCGAAAACAGGGCGTACCTGGACGCATGTCCGGGATGATCGCAATGTGGGTTCGTc                                                     >  1:2806287/3‑150 (MQ=255)

                                                                                              gtGAATGATGCCCTGTACCGTGATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAGAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCa                                                    <  1:3716001/149‑1 (MQ=255)

                                                                                                 aaTGATGCCCTGGACCGTTATGTAATGAATACCCGCAAGGTTCATACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCAtct                                                 <  1:2869221/150‑1 (MQ=255)

                                                                                                   tgatgCCCTGTACCGTTATGTAATGAACACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGtcaacgacggatgaaaagtgatccacttatatctccaccaac                                               >  1:306235‑M1/1‑108 (MQ=255)

                                                                                                     atgCCCTGTACCGTTATGTGATGAATACCCGCAAGCTTCACACTGACGACACACCGGTAAAGGTACTGGCACCGGACCTGAAAAAGACGAAAACAGGGCGCATCTGGACGTATGTCCGGGATGATCGCAATGCGGGTTCGTCATctcctc                                             >  1:185304/1‑150 (MQ=255)

                                                                                                      tgCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCtcctcc                                            >  1:821481/1‑150 (MQ=255)

                                                                                                      tgCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCtcctcc                                            <  1:2672914/150‑1 (MQ=255)

                                                                                                       gCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCtcctcca                                           >  1:3362910/1‑149 (MQ=255)

                                                                                                            gTACCGTTATGTGATGAATACCCGCAAGCTTCACACTGACGACACACCGGTAAAGGTACTGGCACCGGACCTGAAAAAGACGAAAACAGGGCGCATCTGGACGTATGTCCGGGATGATCGCAATGCGGGTTCGTCATCTCCTCCGGCGGt                                      >  1:1539807/1‑150 (MQ=255)

                                                                                                            gTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCCAGCGGt                                      >  1:3207861/1‑150 (MQ=255)

                                                                                                            gTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCCAGCGGt                                      <  1:3492092/150‑1 (MQ=255)

                                                                                                                cGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTgg                                  >  1:2943199/1‑150 (MQ=255)

                                                                                                                      gtaatgaATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTcgcg                            >  1:3313515/4‑150 (MQ=255)

                                                                                                                       taatgaATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGt                           <  1:289270/148‑1 (MQ=255)

                                                                                                                        aatgaATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTa                          >  1:3404459/2‑150 (MQ=255)

                                                                                                                        aatgaATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTa                          >  1:1345974/2‑150 (MQ=255)

                                                                                                                          tgaATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCa                                                                  >  1:1756781/1‑108 (MQ=255)

                                                                                                                          tgaATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCtcctccagc                                         <  1:1638067/133‑4 (MQ=255)

                                                                                                                          tgaATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACt                        >  1:2486560/1‑150 (MQ=255)

                                                                                                                          tgaACACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGtcaacgacggatgaaaagtgatccacttatatctccaccaacggcccaatattgatccaccgttt                        >  1:190523‑M1/1‑85 (MQ=255)

                                                                                                                            aaTACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTcg                      >  1:2720737/1‑149 (MQ=255)

                                                                                                                             aTACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTcgc                     >  1:3768657/1‑148 (MQ=255)

                                                                                                                             aTACCCGCAAGCTTCACACTGACGACACACCGGTAAAGGTACTGGCACCGGACCTGAAAAAGACGAAAACAGGGCGCATCTGGACGTATGTCCGGGATGATCGCAATGCgg                                                            >  1:443627/1‑111 (MQ=255)

                                                                                                                              tACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTcgcc                    <  1:1033897/150‑4 (MQ=255)

                                                                                                                              tACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTcgcc                    <  1:234768/150‑4 (MQ=255)

                                                                                                                              tACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTcgcc                    <  1:2371661/150‑4 (MQ=255)

                                                                                                                               aCCCGCAAGCTTCACACTGACGACACACCGGTAAAGGTACTGGCACCGGACCTGAAAAAGACGAAAACAGGGCGCATCTGGACGTATGTCCGGGATGATCGCAATGCGGGTTCGTCATCTCCTCCGGCGGTCTGGTTCGCGTACTCACCg                   <  1:3734870/150‑1 (MQ=255)

                                                                                                                                cccGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCTAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCAAGCGGTCTGGTTCGCGTACTCGCCGa                  >  1:710833/1‑150 (MQ=255)

                                                                                                                                cccGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGtcaacgacggatgaaaagtgatccacttatatctccatcaacggcccaatattgatccaccgttttactca                  >  1:1616860‑M1/1‑79 (MQ=255)

                                                                                                                                cccGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTCGCCGa                  >  1:150825/1‑150 (MQ=255)

                                                                                                                                cccGCAAGGTTCACACTGATGACACACCGGTAAAAGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGtcaacgacggatgaaaagtgatccacttatatctccaccaacggcccaatattgatccaccgttttactca                  >  1:786467‑M1/1‑79 (MQ=255)

                                                                                                                                 ccGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTCGCCGaa                 <  1:2013610/150‑1 (MQ=255)

                                                                                                                                 ccGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACAGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTCGCCGaa                 >  1:2272219/1‑150 (MQ=255)

                                                                                                                                  cGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGtcaacaacggatgaaaagtgatccacttatatctccaccaacggcccaatattgatccaccgttttactcagg                >  1:1003625‑M1/1‑77 (MQ=255)

                                                                                                                                    cAAGGTTCACACTGATGACACACCGGTAAAGGTAC‑GGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTCGCCGAACCgg             <  1:736413/150‑1 (MQ=255)

                                                                                                                                        gttCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGGTGATCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTCGCCGAACCGGCAg          >  1:383662/2‑150 (MQ=255)

                                                                                                                                        gttCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTCGCCGAACCGGCAg          >  1:2401603/2‑150 (MQ=255)

                                                                                                                                         ttCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTCGCCGAACCGGCAgg         >  1:1230764/1‑150 (MQ=255)

                                                                                                                                             cacTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGaa     <  1:1819078/150‑1 (MQ=255)

                                                                                                                                              acTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGaaa    <  1:2093936/150‑1 (MQ=255)

                                                                                                                                               cTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAac   <  1:1941229/150‑1 (MQ=255)

CGTCAGAGTGAAATCTTTGCCCGACAGGGTGTCGAACTGAGCCGTGCATTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCC‑GCTGAATGATGCCCTGTACCGTTATGTGATGAATACCCGCAAGCTTCACACTGACGACACACCGGTAAAGGTACTGGCACCGGACCTGAAAAAGACGAAAACAGGGCGCATCTGGACGTATGTCCGGGATGATCGCAATGCGGGTTCGTCATCTCCTCCGGCGGTCTGGTTCGCGTACTCACCGAACCGGCAGGGGAAACA  >  Xen14_143/607‑899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`