BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	Xen14_143	742	C→G	intergenic (‑436/–)	Xen14_04759 ← / –	hypothetical protein/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Xen14_143	742	0	C	G	89.0%	2783.8 / 307.6	1088	intergenic (‑436/–)	Xen14_04759/–	hypothetical protein/–
Reads supporting (aligned to +/- strand): ref base C (66/53); new base G (485/479); total (553/535)
Fisher's exact test for biased strand distribution p-value = 3.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

Only 100 of 1088 total aligned reads displayed.

CCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGACAGGGTGTCGAACTGAGCCGTGCATTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCC‑GCTGAATGATGCCCTGTACCGTTATGTGATGAATACCCGCAAGCTTCACACTGACGACACACCGGTAAAGGTACTGGCACCGGACCTGAAAAAGACGAAAACAGGGCGCATCTGGACGTATGTCCGGGATGATCGCAATGCGGGTTCGTCATCTCCTCCGGCGGTCTGGTTCGCGTACTCACCGAACCGG  >  Xen14_143/594‑888

  tGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTc                                                                                                                                                 <  1:2953828/150‑1 (MQ=255)

     cACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAACACCCGCAAGGTTcaca                                                                                                                                              <  1:777838/150‑1 (MQ=255)

       cTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACt                                                                                                                                            <  1:2293876/150‑1 (MQ=255)

       cTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACt                                                                                                                                            >  1:129871/1‑150 (MQ=255)

         gTATCGTCAGAGTGAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTga                                                                                                                                          <  1:355655/150‑1 (MQ=255)

         gTATCGTCAGAGTGAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTga                                                                                                                                          <  1:3570596/150‑1 (MQ=255)

          tATCGTCAGAGTGAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAACACCCGCAAGGTTCACACTgat                                                                                                                                         <  1:1096821/150‑2 (MQ=255)

             cGTCAGAGTGAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTgatgac                                                                                                                                      <  1:55687/150‑1 (MQ=255)

             cGTCAGAGTGAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTgatgac                                                                                                                                      <  1:1618827/150‑1 (MQ=255)

              gTCAGAGTGAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGaca                                                                                                                                     >  1:839740/1‑150 (MQ=255)

              gTCAGAGTGAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGACGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGaca                                                                                                                                     >  1:2254119/1‑150 (MQ=255)

                   agTGAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCg                                                                                                                                >  1:477378/1‑150 (MQ=255)

                   agTGAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCg                                                                                                                                <  1:1605950/150‑1 (MQ=255)

                    gTGAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTGATGAATACCCGCAAGGTTCACACTGATGACACACCgg                                                                                                                               >  1:1394327/1‑150 (MQ=255)

                      gAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTa                                                                                                                             >  1:3608540/1‑150 (MQ=255)

                        aaTCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTaaa                                                                                                                           >  1:2742284/1‑150 (MQ=255)

                         aTCTTTGCCCGCCAGGGTGTCGAACTGAGCCGTGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACATTGATGACACACCGGTAAAg                                                                                                                          <  1:3285101/150‑1 (MQ=255)

                         aTCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAg                                                                                                                          <  1:1445656/150‑1 (MQ=255)

                         aTCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAg                                                                                                                          >  1:3385239/1‑150 (MQ=255)

                          tCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAgg                                                                                                                         >  1:1983093/1‑150 (MQ=255)

                           cTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGt                                                                                                                        >  1:1892582/1‑150 (MQ=255)

                            tttGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTa                                                                                                                       >  1:1499698/1‑150 (MQ=255)

                               gCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACGCACCGGTAAAGGTACTg                                                                                                                    >  1:2041877/1‑150 (MQ=255)

                               gCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTg                                                                                                                    >  1:1228509/1‑150 (MQ=255)

                               gCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTg                                                                                                                    <  1:2445230/150‑1 (MQ=255)

                               gCCCGCCAGGGTGTCGAACTGAGCCGGGCATTACTCTCCAACTGGGTTGAAGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTg                                                                                                                    >  1:3478561/1‑150 (MQ=255)

                                cccGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTgg                                                                                                                   >  1:2726025/1‑150 (MQ=255)

                                 ccgccAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGc                                                                                                                  <  1:595173/146‑1 (MQ=255)

                                    ccAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCAcc                                                                                                               <  1:93128/149‑1 (MQ=255)

                                    ccAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAACACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTGCTGGCAcc                                                                                                               <  1:2469710/149‑1 (MQ=255)

                                       gggTGTCGAGCTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAACACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCggg                                                                                                            <  1:1276762/150‑2 (MQ=255)

                                       gggTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCggg                                                                                                            <  1:2404894/150‑2 (MQ=255)

                                        ggTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCgggt                                                                                                           <  1:2919980/150‑3 (MQ=255)

                                         gtgtCGAACTGAGCCGGGCCTTACTCTTCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCAGC‑GAATGACGCCCTGTACCGTTATGTAATGAACCCCCGCAAAGTTCACACTGATGGCACACCGGTAAAGGTACTGGCACCgggtc                                                                                                          >  1:3685499/1‑147 (MQ=255)

                                          tgtCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGTCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCgggtca                                                                                                         <  1:2345459/150‑5 (MQ=255)

                                          tgtCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCTTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCgggtca                                                                                                         <  1:1138995/150‑5 (MQ=255)

                                           gtCGAACTGAGCCGTGCATTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCC‑GCTGAATGATGCCCTGTACCGTTATGTGATGAATACCCGCAAGCTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGACCTg                                                                                                        >  1:3427293/1‑150 (MQ=255)

                                               aaCTGAGCCGTGCATTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCC‑GCTGAATGATGCCCTGTACCGTTATGTGATGAATACCCGCAAGCTTCACACTGACGACACACCGGTAAAGGTACTGGCACCGGACCTGaaaa                                                                                                    >  1:653987/1‑150 (MQ=255)

                                                aCTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGNACTGGCACCgggtcagaaaaa                                                                                                   <  1:3813400/150‑11 (MQ=255)

                                                  tGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCgggtcagaaaaagg                                                                                                 <  1:25913/150‑13 (MQ=255)

                                                   gAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCTTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCgggtcagaaaaaggc                                                                                                <  1:2537276/150‑14 (MQ=255)

                                                   gAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCgggtcagaaaaaggc                                                                                                <  1:2102840/150‑14 (MQ=255)

                                                   gAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCgggtcagaaaaaggc                                                                                                <  1:3832729/150‑14 (MQ=255)

                                                   gAGCCGGGCCTTACTCTCCAACTGGGTTGACGCATGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGCAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCgggtcagaaaaaggc                                                                                                <  1:2010420/150‑14 (MQ=255)

                                                        gTGCATTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCC‑GCTGAATGATGCCCTGTACCGTTATGTGATGAATACCCGCAAGCTTCACACTGACGACACACCGATAAAGGTACTGGCAcc                                                                                                               >  1:1474306/1‑130 (MQ=255)

                                                          gccttACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAGAGGCGAAAACa                                                                                         >  1:3184816/4‑150 (MQ=255)

                                                          gccttACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACa                                                                                         >  1:1931303/4‑150 (MQ=255)

                                                              tACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACG‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGgc                                                                                     <  1:675407/150‑1 (MQ=255)

                                                                ctctCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCC‑GCTGAATGCTGCCCTGTACCGTTATGTGATGAATACCCGCAAGTTTCACACTGACGACACACCGGTAAAGGTACTGGCACCGGACCTGAAAAAGACGAAAACAGGgcgc                                                                                   >  1:3060375/1‑150 (MQ=255)

                                                                ctctCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGgcgt                                                                                   >  1:410796/1‑149 (MQ=255)

                                                                  ctcCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCC‑GCTGAATGATGCCCTGTACCGTTATGTGATGAATACCCGCAAGCTTCACACTGACGACACACCGGTAAAGGTACTGGCACCGGACCTGAAAAAGACGAAAACAGGgcgtat                                                                                 >  1:3782938/1‑147 (MQ=255)

                                                                     cAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTg                                                                              <  1:3250813/150‑1 (MQ=255)

                                                                     cAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTg                                                                              <  1:3100808/150‑1 (MQ=255)

                                                                     cAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAACACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTg                                                                              <  1:2213688/150‑1 (MQ=255)

                                                                      aaCTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACGCACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTgg                                                                             >  1:1872692/1‑150 (MQ=255)

                                                                       aCTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTATTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGa                                                                            >  1:2134851/1‑150 (MQ=255)

                                                                        cTGGGTTGACGTGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAACACCCGCAAGGTTCACACTGATGACACACCGTTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTgtca                                                                           <  1:2674731/150‑4 (MQ=255)

                                                                        cTGGGTTGACGCGCACTGCCAGTCAACGACGCC‑ACTGACTGATGCCCTATACCGCTATGTGATGAATACCCGCAAGCTTCACACTGACGACACACCAGTAAAGGTACTGGCACCGGACCTGAAAAAGACGAAAACAGGGCGCATCTGGAc                                                                           >  1:2853223/1‑150 (MQ=255)

                                                                         tGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACg                                                                          <  1:3715708/150‑1 (MQ=255)

                                                                         tGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACg                                                                          <  1:2487330/150‑1 (MQ=255)

                                                                          gggTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGt                                                                         >  1:3728808/1‑150 (MQ=255)

                                                                            gTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTGCTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTAt                                                                       >  1:2876066/1‑150 (MQ=255)

                                                                             ttGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATg                                                                      <  1:3217416/150‑1 (MQ=255)

                                                                                 cgcgTGCTGCCAGTTAATGCCACC‑GGCGAATGATGCCCTGTACCGTTATGTACTGAATACCCGCAAGGTTCCCACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCg                                                                  >  1:260271/1‑150 (MQ=255)

                                                                                 cgcgTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAGACAGGGCGTATCTGGACGTATGTCCg                                                                  >  1:1340513/1‑150 (MQ=255)

                                                                                  gcgTGCTGCCAGTTAATGACATC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCgg                                                                 >  1:2166070/1‑150 (MQ=255)

                                                                                   cgTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCggg                                                                <  1:2357195/150‑1 (MQ=255)

                                                                                    gTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGga                                                               <  1:1743538/150‑1 (MQ=255)

                                                                                    gTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCAGGACGTATGTCCGGga                                                               <  1:509370/150‑1 (MQ=255)

                                                                                       ctgcCAGTTAATGACGCC‑GCTGAATGATGCCCTGTACCGTTATGTGATGAATACCCGCAAGCTTCACACTGACGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGgatga                                                            >  1:1765260/1‑150 (MQ=255)

                                                                                           cAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGc                                                        >  1:1831227/1‑150 (MQ=255)

                                                                                            aGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGGTCGCa                                                       >  1:879979/1‑150 (MQ=255)

                                                                                                 aTGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATgtg                                                  >  1:444495/1‑148 (MQ=255)

                                                                                                 aTGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATgtg                                                  >  1:2821325/1‑148 (MQ=255)

                                                                                                      caccggtGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCGAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACg                                                                          >  1:3456900/7‑122 (MQ=255)

                                                                                                      caccggtGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGtt                                              >  1:3342745/7‑150 (MQ=255)

                                                                                                      caccggtGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGtt                                              >  1:2635117/7‑150 (MQ=255)

                                                                                                        ccggtGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCg                                            <  1:8112/146‑1 (MQ=255)

                                                                                                               atgatgCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCAtctc                                     <  1:250551/150‑1 (MQ=255)

                                                                                                                    gCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCtcctcca                                <  1:3014875/150‑2 (MQ=255)

                                                                                                                    gCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCtcctcca                                <  1:3766312/150‑2 (MQ=255)

                                                                                                                       cTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCcagcg                             <  1:3517133/150‑1 (MQ=255)

                                                                                                                        tGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCcagcgg                            <  1:1029896/150‑1 (MQ=255)

                                                                                                                            ccGTTATGTAATGAACACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGtcaacgacggatgaaaagtgatccacttatatctccaccaacggcccaatattg                        >  1:2307809‑M1/1‑96 (MQ=255)

                                                                                                                                 aTGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTcg                   >  1:1055860/1‑150 (MQ=255)

                                                                                                                                  tgtaatgaATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGtttgc                  <  1:1800769/146‑4 (MQ=255)

                                                                                                                                   gtaatgaATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTcgcg                 <  1:1699619/147‑1 (MQ=255)

                                                                                                                                   gtaatgaATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTcgcg                 >  1:1553602/4‑150 (MQ=255)

                                                                                                                                       tgaATACCCGCAAGGTTCACACCGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACt             >  1:2074887/1‑150 (MQ=255)

                                                                                                                                        gaATACCCGTGAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTAc              <  1:3641051/148‑1 (MQ=255)

                                                                                                                                          aTACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTcg                   <  1:2788516/141‑1 (MQ=255)

                                                                                                                                           tACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTcgcc         <  1:1960961/150‑4 (MQ=255)

                                                                                                                                             cccGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCTAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCAAGCGGTCTGGTTCGCGTACTCGCCGa       >  1:710832/1‑150 (MQ=255)

                                                                                                                                             cccGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTCGCCGa       >  1:3164890/1‑150 (MQ=255)

                                                                                                                                             cccGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTCGCCGa       >  1:2895199/1‑150 (MQ=255)

                                                                                                                                             cccGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCCAGCGATCTGGTTCGCGTACTCGCCGa       <  1:3130945/150‑1 (MQ=255)

                                                                                                                                               cGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGtcaacaacggatgaaaagtgatccacttatatctccaccaacggcccaatattgatccaccgttttactcagg     >  1:1003625‑M1/1‑77 (MQ=255)

                                                                                                                                               cGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTCGCCGAAc     >  1:1646107/1‑150 (MQ=255)

                                                                                                                                               cGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCATATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTCGCCGAAc     <  1:1188614/150‑1 (MQ=255)

                                                                                                                                                 cAAGGTTCACACTGATGACACACCGGTAAAGGTAC‑GGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTCGCCGAACCgg  <  1:736413/150‑1 (MQ=255)

CCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGACAGGGTGTCGAACTGAGCCGTGCATTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCC‑GCTGAATGATGCCCTGTACCGTTATGTGATGAATACCCGCAAGCTTCACACTGACGACACACCGGTAAAGGTACTGGCACCGGACCTGAAAAAGACGAAAACAGGGCGCATCTGGACGTATGTCCGGGATGATCGCAATGCGGGTTCGTCATCTCCTCCGGCGGTCTGGTTCGCGTACTCACCGAACCGG  >  Xen14_143/594‑888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`