BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	Xen14_143	726	G→A	intergenic (‑420/–)	Xen14_04759 ← / –	hypothetical protein/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Xen14_143	726	0	G	A	88.6%	2294.7 / 179.8	1029	intergenic (‑420/–)	Xen14_04759/–	hypothetical protein/–
Reads supporting (aligned to +/- strand): ref base G (70/47); new base A (467/445); total (537/492)
Fisher's exact test for biased strand distribution p-value = 9.44e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 1067 total aligned reads displayed.

GGAAAATACTGCGAACACCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGACAGGGTGTCGAACTGAGCCGTGCATTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCC‑GCTGAATGATGCCCTGTACCGTTATGTGATGAATACCCGCAAGCTTCACACTGACGACACACCGGTAAAGGTACTGGCACCGGACCTGAAAAAGACGAAAACAGGGCGCATCTGGACGTATGTCCGGGATGATCGCAATGCGGGTTCGTCATCTCCTCCGGCGGTCTGGTTCGCGT  >  Xen14_143/577‑874

    aaTACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGtaatga                                                                                                                                                  <  1:1060765/150‑1 (MQ=255)

      tACTGCGAACACCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGACAGGGTGTCGAACTGAGCCGTGCATTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCC‑GCTGAATGATGCCCTGTACCGTTATGTGATGAAt                                                                                                                                                <  1:3722509/150‑1 (MQ=255)

        cTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGaacac                                                                                                                                              >  1:1015869/1‑147 (MQ=255)

        cTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTATACCGTTATGTAATGAATAc                                                                                                                                              >  1:3069546/1‑150 (MQ=255)

          gCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATAccc                                                                                                                                            >  1:1039209/1‑150 (MQ=255)

           cGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCg                                                                                                                                           <  1:2245662/150‑1 (MQ=255)

           cGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAACACCCg                                                                                                                                           >  1:132575/1‑150 (MQ=255)

           cGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGATGCCAGTTAATGACACC‑GGTAAATGATGCCCTGTACCGTTATGTAATGAACACCCg                                                                                                                                           >  1:275350/1‑150 (MQ=255)

              acatcTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCaa                                                                                                                                        >  1:251286/5‑150 (MQ=255)

              acatcTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCaa                                                                                                                                        >  1:1897165/5‑150 (MQ=255)

                atcTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACTGTTATGTAATGAATACCCGCAAgg                                                                                                                                      >  1:2468579/3‑149 (MQ=255)

                atcTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAgg                                                                                                                                      <  1:1191/148‑2 (MQ=255)

                  cTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAATTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAggtt                                                                                                                                    >  1:2526352/1‑147 (MQ=255)

                   tGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTc                                                                                                                                   >  1:3049543/1‑150 (MQ=255)

                   tGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCATTATGTAATGAATACCCGcgaggttc                                                                                                                                   <  1:517062/150‑8 (MQ=255)

                   tGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCAAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTc                                                                                                                                   >  1:2653752/1‑150 (MQ=255)

                     ccACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTcac                                                                                                                                 <  1:481058/150‑1 (MQ=255)

                     ccACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTcac                                                                                                                                 <  1:943902/150‑1 (MQ=255)

                     ccACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTcac                                                                                                                                 >  1:1365537/1‑150 (MQ=255)

                      cACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAACACCCGCAAGGTTcaca                                                                                                                                <  1:777837/150‑1 (MQ=255)

                       aCTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGTGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCacac                                                                                                                               <  1:3265974/150‑1 (MQ=255)

                        cTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACt                                                                                                                              <  1:2293876/150‑1 (MQ=255)

                           tATCGTCAGAGTGAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAACACCCGCAAGGTTCACACTgat                                                                                                                           <  1:1096821/150‑2 (MQ=255)

                            aTCGTCAGAGTGAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTgatg                                                                                                                          >  1:880700/1‑148 (MQ=255)

                             tCGTCAGAGTGAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTgatga                                                                                                                         <  1:299635/150‑4 (MQ=255)

                              cGTCAGAGTGAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTCATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTgatgac                                                                                                                        >  1:1513926/1‑150 (MQ=255)

                              cGTCAGAGTGAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTgatgac                                                                                                                        >  1:1572402/1‑150 (MQ=255)

                              cGTCAGAGTGAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAACACCCGCAAGGTTCACACTgatgac                                                                                                                        >  1:829668/1‑150 (MQ=255)

                                     gTGAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCgg                                                                                                                 >  1:3438156/1‑150 (MQ=255)

                                       gAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTa                                                                                                               >  1:3608540/1‑150 (MQ=255)

                                       gAAATCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTa                                                                                                               <  1:1987853/150‑1 (MQ=255)

                                       gAAATCTTTGCCCGACAGGGTGTCGAACTGAGCCGTGCATTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCC‑GCTGAATGATGCCCTGTACCGTTATGTGATGAATACCCGCAAGCTTCACACTGACGACACACCGGTa                                                                                                               >  1:2366805/1‑150 (MQ=255)

                                          aTCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAg                                                                                                            >  1:2094326/1‑150 (MQ=255)

                                           tCTTTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAgg                                                                                                           <  1:1416635/150‑1 (MQ=255)

                                             tttGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTa                                                                                                         >  1:867707/1‑150 (MQ=255)

                                             tttGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTa                                                                                                         >  1:2907602/1‑150 (MQ=255)

                                             tttGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTa                                                                                                         <  1:2345867/150‑1 (MQ=255)

                                             tttGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTa                                                                                                         >  1:575987/1‑150 (MQ=255)

                                              ttGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAACACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTAc                                                                                                        >  1:1686107/1‑150 (MQ=255)

                                                gCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTaaa                                                                                                             <  1:161556/143‑1 (MQ=255)

                                                gCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTg                                                                                                      <  1:2445230/150‑1 (MQ=255)

                                                 cccGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTgg                                                                                                     <  1:410904/150‑1 (MQ=255)

                                                  ccgccAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGc                                                                                                    <  1:2837557/146‑1 (MQ=255)

                                                  ccGACAGGGTGTCGAACTGAGCCGTGCATTACTTTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCC‑GCTGAATGATGCCCTGTACCGTTATGTGATGAATACCCGCAAGCTTCACACTGACGACACACCGGTAAAGGTACTGGc                                                                                                    <  1:613960/150‑1 (MQ=255)

                                                  ccGACAGGGTGTCGAACTGAGCCGTGCATTACTTTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCC‑GCTGAATGATGCCCTGTACCGTTATGTGATGAATACCCGCAAGCTTCACACTGACGACACACCGGTAAAGGTACTGGc                                                                                                    <  1:2119144/150‑1 (MQ=255)

                                                          gtgtCGAACTGAGCCGGGCCTTACTCTTCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCAGC‑GAATGACGCCCTGTACCGTTATGTAATGAACCCCCGCAAAGTTCACACTGATGGCACACCGGTAAAGGTACTGGCACCgggtc                                                                                            >  1:3685499/1‑147 (MQ=255)

                                                           tgtCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCTTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCgggtca                                                                                           <  1:1138995/150‑5 (MQ=255)

                                                           tgtCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAACGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCgggtca                                                                                           >  1:702955/1‑146 (MQ=255)

                                                            gtCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCgggtcag                                                                                          <  1:2560429/150‑6 (MQ=255)

                                                             tCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCgggtcaga                                                                                         >  1:1820297/1‑144 (MQ=255)

                                                                  cTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACACGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCgggtcagaaaaag                                                                                    <  1:1482975/150‑12 (MQ=255)

                                                                   tGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGAGGACACACCGGTAAAGGTACTGGCACCgggtcagaaaaagg                                                                                   <  1:3748542/150‑13 (MQ=255)

                                                                       ccgggccttACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGaa                                                                               >  1:2492843/8‑150 (MQ=255)

                                                                        cgggccttACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACATACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGaaa                                                                              <  1:1784009/144‑1 (MQ=255)

                                                                          ggccttACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGCTGACACACCGGTAAAAGTACTGGCACCGGGTCAGAAAAAGGCGAAAAc                                                                            >  1:1450788/5‑150 (MQ=255)

                                                                           gccttACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGacac                                                                                                                      >  1:1236006/4‑107 (MQ=255)

                                                                           gccttACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAGAGGCGAAAACa                                                                           >  1:3184816/4‑150 (MQ=255)

                                                                            cATTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCC‑GCTGAATGATGCCCTGTACCGTTATGTGATGAATACCCGCAAGCTTCACACTGACGACACACCGGTAAAGGTACTGGCACCGGACCTGAAAGAGACGAAAACAg                                                                          <  1:201224/150‑1 (MQ=255)

                                                                            cATTACTCTCCAACTGGGTTGACGCATGCTGCCAGTTAATGACGCC‑GCTGAATGATGCCCTGTACCGTTATGTGATGAATACCCGCAAGCTTCACACTGACGACACACCGGTAAAGGTACTGGCACCGGACCTGAAAAAGACGAAAACAg                                                                          >  1:2933143/1‑150 (MQ=255)

                                                                              ttACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAACGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAggg                                                                        <  1:1635430/150‑1 (MQ=255)

                                                                              ttACCCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAngg                                                                        <  1:219977/150‑1 (MQ=255)

                                                                               tACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGgc                                                                       >  1:3566925/1‑150 (MQ=255)

                                                                                aCTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTNAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGgcg                                                                      >  1:3549530/1‑150 (MQ=255)

                                                                                aCTCTCCAACTGGGGTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGgcg                                                                      <  1:1969889/150‑1 (MQ=255)

                                                                                    tcCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GCTGAATGATGCCCTGTACCGTTATGTGATGAATACCCGCAAGCTTCACGCTGACGACACACCGGTAAAGGTACTGGCACCGGACCTGAAAAAGACGAAAACAGGGCGCATc                                                                  <  1:1711531/150‑1 (MQ=255)

                                                                                        aCTGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGa                                                              <  1:3120226/150‑1 (MQ=255)

                                                                                          tGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACg                                                            <  1:2481192/150‑1 (MQ=255)

                                                                                          tGGGTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACg                                                            <  1:3715708/150‑1 (MQ=255)

                                                                                             gTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTGCTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTAt                                                         >  1:2876066/1‑150 (MQ=255)

                                                                                             gTTGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTAt                                                         >  1:3410971/1‑150 (MQ=255)

                                                                                              ttGACGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATg                                                        <  1:3217417/150‑1 (MQ=255)

                                                                                                 aCGCGTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTcc                                                     >  1:2693764/1‑150 (MQ=255)

                                                                                                    cgTGCTGCCAGTTAATGACGCC‑GCTGAATGATGCCCTGTACCGTTATGTGATGAATACCCGCAAGCTTCACACTGACGACACACCGGTAAAGGTACTGGCACCGGACCTGAAAAAGACGAAAACAGGGCGCATCTGGACGTATGTCCggg                                                  >  1:541642/1‑150 (MQ=255)

                                                                                                    cgTGCTGCCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCCGGACGTATGTCCggg                                                  <  1:387010/150‑1 (MQ=255)

                                                                                                      tgctgcCAGTTAATGACGCC‑GCTGAATGATGCCCTGTACCGTTATGTGATGAATACCCGCAAGCTTCACACTGACGACACACCGGTAAAGGTACTGGCACCGGACCTGAAAAAGACGAAAACAGGGCGCATCTGGACGTATGTCCGGgat                                                <  1:733638/150‑1 (MQ=255)

                                                                                                       gctgcCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGgatg                                               <  1:1931874/150‑1 (MQ=255)

                                                                                                       gctgcCAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGgatg                                               <  1:2857708/150‑1 (MQ=255)

                                                                                                        ctgcCAGTTAATGACGCC‑GCTGAATGATGCCCTGTACCGTTATGTGATGAATACCCGCAAGCTTCACACTGACGACACACCGGTAAAGGTACTGGCACCGGACCTGAAAAAGACGAAAACAGGGCGCATCTGGACGTATGTCCGGgatga                                              >  1:3369619/1‑150 (MQ=255)

                                                                                                           ccAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCg                                           <  1:3313436/150‑1 (MQ=255)

                                                                                                           ccAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCg                                           <  1:666146/150‑1 (MQ=255)

                                                                                                            cAGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGc                                          >  1:3641447/1‑150 (MQ=255)

                                                                                                             aGTTAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAGGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGAc                                                             <  1:443153/130‑1 (MQ=255)

                                                                                                               ttAATGACACC‑GGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAAt                                       <  1:315134/150‑1 (MQ=255)

                                                                                                                         ccggtGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCg                              <  1:8112/146‑1 (MQ=255)

                                                                                                                          cggtGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACAGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGt                             >  1:2792095/4‑150 (MQ=255)

                                                                                                                             tGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCAt                          <  1:2055369/150‑1 (MQ=255)

                                                                                                                                atgatgCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCAtctc                       >  1:2152902/1‑150 (MQ=255)

                                                                                                                                   atgCCCTGTACCGTTATGTAATGAACACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGtcaacgacggatgaaaagtgatccacttatatctccaccaacgg                    >  1:1875587‑M1/1‑106 (MQ=255)

                                                                                                                                     gCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCtcctcca                  >  1:3477358/1‑149 (MQ=255)

                                                                                                                                        cTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCcagcg               <  1:1285535/150‑1 (MQ=255)

                                                                                                                                         tGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTTATCTCCTCcagcgg              <  1:3499178/150‑1 (MQ=255)

                                                                                                                                         tGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCcagcgg              <  1:2407932/150‑1 (MQ=255)

                                                                                                                                            aCCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCg                                           >  1:3807756/1‑118 (MQ=255)

                                                                                                                                             ccGTTATGTAATGAACACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGtcaacgacggatgaaaagtgatccacttatatctccaccaacggcccaatattg          >  1:2307809‑M1/1‑96 (MQ=255)

                                                                                                                                              cGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTgg         >  1:3777478/1‑150 (MQ=255)

                                                                                                                                               gTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGgatgat                                             <  1:1845052/113‑1 (MQ=255)

                                                                                                                                               gTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGt        >  1:2729345/1‑150 (MQ=255)

                                                                                                                                                ttATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGtt       <  1:2592648/150‑1 (MQ=255)

                                                                                                                                                  aTGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTcg     <  1:1750611/150‑1 (MQ=255)

                                                                                                                                                     tgatgaATACCCGCAAGCTTCACACTGACGACACACCGGTAAAGGTACTGGCACCGGACCTGAAAAAGACGAAAACAGGGCGCATCTGGACGTATGTCCGGGATGATCGCAATGCGGGTTCGTCATCTCCTCCGGCGGTCTGGTTCGCGt  >  1:2630533/1‑150 (MQ=255)

GGAAAATACTGCGAACACCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGACAGGGTGTCGAACTGAGCCGTGCATTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCC‑GCTGAATGATGCCCTGTACCGTTATGTGATGAATACCCGCAAGCTTCACACTGACGACACACCGGTAAAGGTACTGGCACCGGACCTGAAAAAGACGAAAACAGGGCGCATCTGGACGTATGTCCGGGATGATCGCAATGCGGGTTCGTCATCTCCTCCGGCGGTCTGGTTCGCGT  >  Xen14_143/577‑874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`