Predicted mutation
evidence seq id position mutation annotation gene description
RA Xen14_143 136 G→A S57S (AGC→AGT)  Xen14_04759 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Xen14_1431360GA82.3% 1517.0 / 255.6 818S57S (AGC→AGT) Xen14_04759hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (67/78);  new base A (334/339);  total (401/417)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

Only 100 of 818 total aligned reads displayed.

‑‑‑‑‑‑‑‑‑CATTGCAGAACTGGAAAGCCAGCTCCGTCAGAAAGACCAGCAACTGAGTCTGGTTGAAGAGACGGAGGCCTTCCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACAGGCTGAGGCCCTGCTGAAACAACGCGAACAGGACAGCGATCGTTACAGTGGGCGGGAAGACGATCCACAGGTTC  >  Xen14_143/1‑280
                                                                                                                                                |                                                                                                                                                
cgcattgcagaactggaaagccagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAg                                                                                                                                             >  1:3129718‑M1/83‑150 (MQ=255)
 gcattgcagaactggaaagccagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGa                                                                                                                                            <  1:2953518‑M1/69‑1 (MQ=255)
   attgcagaactggaaagccagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATa                                                                                                                                          <  1:3156989‑M1/71‑1 (MQ=255)
   attgcagaactggaaagccagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATa                                                                                                                                          <  1:1969749‑M1/71‑1 (MQ=255)
    attcgcATTGCAGAACTGGAAAGCCAGCTCCGTCAGAAAGACCAGCAACTGAGTCTGGTTGAAGAGACGGAGGCCTTCCTGCACTGGCGCGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAg                                                                                                                                         <  1:1026666/145‑1 (MQ=255)
    attcgcATTGCAGAACTGGAAAGCCAGCTCCGTCAGAAAGAACAGCAACTGAGTCTGGTTGAAGAGACGGAGGCCTTCCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAg                                                                                                                                         >  1:797933/6‑150 (MQ=255)
       cagaactggaaagccagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGaaa                                                                                                                                      >  1:2591325‑M1/76‑150 (MQ=255)
        agaactggaaagccagctccgtcagaaagaccagcagctgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGaaaa                                                                                                                                     >  1:498240‑M1/75‑150 (MQ=255)
        agaactggaaagccagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGaaaa                                                                                                                                     >  1:1000079‑M1/75‑150 (MQ=255)
        agaactggaaagccagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGaaaa                                                                                                                                     >  1:1957328‑M1/75‑150 (MQ=255)
        agaactggaaagccagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACCGGAAATAGAACATCTGCGGACTCAGATAGaaaa                                                                                                                                     >  1:2022833‑M1/75‑150 (MQ=255)
         gaactggaaagccagctccgtcagaaagaccagcaacttagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAg                                                                                                                                         <  1:686959‑M1/72‑1 (MQ=255)
         gaactggaaagccagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAg                                                                                                                                         <  1:980641‑M1/72‑1 (MQ=255)
         gaactggaaagccagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAg                                                                                                                                         <  1:3754974‑M1/72‑1 (MQ=255)
         gaactggaaagccagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAg                                                                                                                                         <  1:1233885‑M1/72‑1 (MQ=255)
            ctggaaagccagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAAAAAAACTg                                                                                                                                 <  1:2636375‑M1/80‑1 (MQ=255)
              cAGAACTGGAAAGCCAGCTCCGTCAGAAAGACCAGCAACTGAGTCTGGTTGAAGAGACGGAGGCCTTCCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAGCTgcg                                                                                                                               >  1:662910/1‑150 (MQ=255)
                gAACTGGAAAGCCAGCTCCGTCAGAAAGACCAGCAACTGAGTCTGGTTGAAGAGACGGAGGCCTTCCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGcc                                                                                                                             >  1:83998/1‑150 (MQ=255)
                 aagccagctccgtcagaaagaccagcaactgagtctggttgaagagacggatgccttcctgcgctCTGCACTGGCACGTGCCGAAGAAGAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAaactgcaccg                                                                                                                            >  1:2840406‑M1/66‑146 (MQ=255)
                   gccagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGa                                                                                                                          <  1:1274797‑M1/87‑1 (MQ=255)
                   gccagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGa                                                                                                                          <  1:1373202‑M1/87‑1 (MQ=255)
                    ccagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGAt                                                                                                                         >  1:563946‑M1/63‑150 (MQ=255)
                     cagctccgtcagaaagaccagcaactgagtctggttgaagagacgggggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACANCTGCGGACTCAGATAGAAAAACTGCACCGGATg                                                                                                                        <  1:1777076‑M1/89‑1 (MQ=255)
                       gctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCt                                                                                                                      <  1:2169016‑M1/91‑1 (MQ=255)
                          ccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGtt                                                                                                                   <  1:2895817‑M1/94‑1 (MQ=255)
                           cgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTc                                                                                                                  <  1:1740973‑M1/95‑1 (MQ=255)
                            gtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCg                                                                                                                 <  1:784196‑M1/96‑1 (MQ=255)
                               agaaagaccagcagctgagtctggttgaagggacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTa                                                                                                              >  1:2053813‑M1/52‑150 (MQ=255)
                               agaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTa                                                                                                              <  1:2257910‑M1/99‑1 (MQ=255)
                                gaaagaccagcaactgagtctggttgaagagacggaggccttcccgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTAc                                                                                                             >  1:916815‑M1/51‑150 (MQ=255)
                                gaaagaccagcaactgagtctgggtgaagagacggatgccttcctgcgctCTGCACTGGCACGGGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTAc                                                                                                             >  1:187986‑M1/51‑150 (MQ=255)
                                   agaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCTAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCg                                                                                                          >  1:3777073‑M1/48‑150 (MQ=255)
                                    gaccagcaactgattctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGt                                                                                                         >  1:3489165‑M1/47‑150 (MQ=255)
                                      ccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTc                                                                                                       <  1:1116121‑M1/106‑1 (MQ=255)
                                       cagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCTGATGCTGTTCGGTACCCGTTCt                                                                                                      >  1:2225613‑M1/44‑150 (MQ=255)
                                       cagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCt                                                                                                      >  1:1343737‑M1/44‑150 (MQ=255)
                                       cagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCt                                                                                                      >  1:2939200‑M1/44‑150 (MQ=255)
                                        agcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGc                                                                                                                       <  1:11533‑M1/90‑1 (MQ=255)
                                         gcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGa                                                                                                    >  1:3222488‑M1/42‑150 (MQ=255)
                                           aactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGaaa                                                                                                  >  1:1613664‑M1/40‑150 (MQ=255)
                                            actgagtctggttgaagagacggatgccctcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATTGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGaaaa                                                                                                 >  1:3590780‑M1/39‑150 (MQ=255)
                                            actgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGaaaa                                                                                                 >  1:1071861‑M1/39‑150 (MQ=255)
                                            actgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGaaaa                                                                                                 >  1:2097484‑M1/39‑150 (MQ=255)
                                            actgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGaaaa                                                                                                 >  1:1593009‑M1/39‑150 (MQ=255)
                                              tgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACTGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAAc                                                                                               >  1:3634187‑M1/37‑150 (MQ=255)
                                              tgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAAc                                                                                               >  1:2725655‑M1/37‑150 (MQ=255)
                                                agtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTg                                                                                             >  1:1298031‑M1/35‑150 (MQ=255)
                                                 gtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGc                                                                                            <  1:616391‑M1/117‑1 (MQ=255)
                                                 gtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGc                                                                                            <  1:2387671‑M1/117‑1 (MQ=255)
                                                 cAACTGAGTCTGGTTGAAGAGACGGAGGCCTTCCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCAGAAAAACTGc                                                                                            <  1:1494660/150‑1 (MQ=255)
                                                  tctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGcg                                                                                           >  1:2189108‑M1/33‑150 (MQ=255)
                                                    tggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGcgtc                                                                                         <  1:1395718‑M1/120‑1 (MQ=255)
                                                    tggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGcgtc                                                                                         <  1:734307‑M1/120‑1 (MQ=255)
                                                     tGAGTCTGGTTGAAGAGACGGAGGCCTTCCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGcgtcg                                                                                        >  1:1306713/1‑150 (MQ=255)
                                                     ggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGcgtcg                                                                                        <  1:2771006‑M1/121‑1 (MQ=255)
                                                      gttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGcgtcgt                                                                                       <  1:3398746‑M1/122‑1 (MQ=255)
                                                       ttgaagagacggaggctttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTg                                                                                      <  1:2454826‑M1/123‑1 (MQ=255)
                                                        tgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGa                                                                                     <  1:2500471‑M1/124‑1 (MQ=255)
                                                        tgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACACCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGa                                                                                     <  1:3377294‑M1/124‑1 (MQ=255)
                                                         gaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGaa                                                                                    <  1:2672902‑M1/125‑1 (MQ=255)
                                                         gaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGaa                                                                                    <  1:3079086‑M1/125‑1 (MQ=255)
                                                          aagagacggaggccttcctgcgctCTGCATTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAg                                                                                   >  1:124754‑M1/25‑150 (MQ=255)
                                                          aagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAg                                                                                   <  1:873921‑M1/126‑1 (MQ=255)
                                                           tGGTTGAAGAGACGGAGGCCTTCCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGt                                                                                  <  1:3264497/150‑1 (MQ=255)
                                                           agagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAagg                                                                                  >  1:3678764‑M1/24‑149 (MQ=255)
                                                           agagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGt                                                                                  <  1:3833307‑M1/127‑1 (MQ=255)
                                                            gagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGACAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGtt                                                                                 <  1:1182614‑M1/128‑1 (MQ=255)
                                                              gacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGa                                                                               >  1:1507592‑M1/21‑150 (MQ=255)
                                                              gacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGa                                                                               <  1:2693536‑M1/130‑1 (MQ=255)
                                                               acggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGaa                                                                              >  1:52804‑M1/20‑150 (MQ=255)
                                                                gAAGAGACGGAGGCCTTCCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAAc                                                                             <  1:399070/150‑1 (MQ=255)
                                                                cggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGAGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAAc                                                                             >  1:1845103‑M1/19‑150 (MQ=255)
                                                                cggaggccttactgcgctCTGCACTGGCACGTGCCGAAGAAAGGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAAc                                                                             >  1:3177131‑M1/19‑150 (MQ=255)
                                                                   gagaCGGAGGCCTTCCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACAgg                                                                          >  1:2797525/1‑150 (MQ=255)
                                                                   aggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGaaaaa                                                                                                >  1:1713612‑M1/16‑128 (MQ=255)
                                                                     gccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCt                                                                        >  1:1432718‑M1/14‑150 (MQ=255)
                                                                       cttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGa                                                                      >  1:3794633‑M1/12‑150 (MQ=255)
                                                                          cctgcgctTTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGACGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGc                                                                   >  1:3282580‑M1/9‑150 (MQ=255)
                                                                          cctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGc                                                                   >  1:1547974‑M1/9‑150 (MQ=255)
                                                                           ggCCTTCCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACAGGCTGAGGcc                                                                  >  1:3444342/1‑150 (MQ=255)
                                                                           ctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAactggctcccatg                                                                  <  1:1460788‑M1/143‑7 (MQ=255)
                                                                             gcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGACCGGGAAATAGAATATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGaggctct                                                                >  1:3728291‑M1/6‑147 (MQ=255)
                                                                               gctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGaaa                                                                                                  <  1:1640595‑M1/111‑1 (MQ=255)
                                                                               gctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGCTGAACTGGCTGAGGCTctgc                                                              <  1:1997546‑M1/147‑1 (MQ=255)
                                                                                 ccTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACAGGCTGAGGCCctgctg                                                            <  1:2137100/150‑1 (MQ=255)
                                                                                   tGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACAGGCTGAGGCCCTGCTGaa                                                          <  1:3329400/150‑1 (MQ=255)
                                                                                   tGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTCTGCTGaa                                                          >  1:2332704/1‑150 (MQ=25)
                                                                                   tGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTCTGCTGaa                                                          <  1:7077/150‑1 (MQ=25)
                                                                                   tGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGAATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTCTGCTGaa                                                          <  1:469001/150‑1 (MQ=25)
                                                                                    gCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAATATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTCTGCTGaaa                                                         <  1:2513391/150‑1 (MQ=25)
                                                                                    gCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTCTGCTGaaa                                                         >  1:2829817/1‑150 (MQ=25)
                                                                                       cTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACGTCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTCTGCTGAaacaa                                                      >  1:2903125/1‑150 (MQ=25)
                                                                                       cTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTCTGCTGAaacaa                                                      >  1:450362/1‑150 (MQ=25)
                                                                                         ggCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTCTGCTGAAACAAcg                                                    >  1:2550439/1‑150 (MQ=25)
                                                                                              gTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACAGGCTGAGGCCCTGCTGAAACAACGCGAAc                                               <  1:3429554/150‑1 (MQ=255)
                                                                                                          aGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACAGGCTGAGGCCCTGCTGAAACAACGCGAACAGGACAGCGATc                                   <  1:3560836/150‑1 (MQ=255)
                                                                                                              cGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACAGGCTGAGGCCCTGCTGAAACAACGCGAACAGGACAGCGATCGTTa                               >  1:1809823/1‑150 (MQ=255)
                                                                                                               gaagaaGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACAGGCTGAGGCCCTGCTGAAACAACGCGAACAGGACAGCGATCGTTAc                              <  1:3021964/150‑1 (MQ=255)
                                                                                                                 agaagaTGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACAGGCTGAGGCCCTGCTGAAACAACGCGAACAGGACAGCGATCGTTACAg                            <  1:3512169/150‑1 (MQ=255)
                                                                                                                                       cATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACAGGCTGAGGCCCTGCTGAAACAACGCGAACAGGACAGCGATCGTTACAGTGGGCGGGAAGACGATCCACAg      <  1:1904335/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
‑‑‑‑‑‑‑‑‑CATTGCAGAACTGGAAAGCCAGCTCCGTCAGAAAGACCAGCAACTGAGTCTGGTTGAAGAGACGGAGGCCTTCCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACAGGCTGAGGCCCTGCTGAAACAACGCGAACAGGACAGCGATCGTTACAGTGGGCGGGAAGACGATCCACAGGTTC  >  Xen14_143/1‑280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑