Predicted mutation
evidence seq id position mutation annotation gene description
RA Xen14_126 2,053 G→T P89P (CCG→CCT)  Xen14_04726 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Xen14_1262,0530GT80.6% 2010.0 / inf 911P89P (CCG→CCT) Xen14_04726hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (62/114);  new base T (358/374);  total (422/489)
Fisher's exact test for biased strand distribution p-value = 1.33e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 916 total aligned reads displayed.

AATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGTGTTGATCCGCGAACTGAGTGGG  >  Xen14_126/1904‑2194
                                                                                                                                                     |                                                                                                                                                   
aaTGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCg                                                                                                                                                     >  1:71531/1‑150 (MQ=255)
   ggCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCtgtg                                                                                                                                                  >  1:3456572/1‑150 (MQ=255)
   ggCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATGATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCtgtg                                                                                                                                                  <  1:1058542/150‑1 (MQ=255)
      gTCAATGATAATGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGgct                                                                                                                                               <  1:1668748/150‑1 (MQ=255)
        cAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggc                                                                                                                                             <  1:1973118/150‑4 (MQ=255)
        cAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggc                                                                                                                                             >  1:607094/1‑147 (MQ=255)
          aTGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggcca                                                                                                                                           <  1:1590208/150‑6 (MQ=255)
          aTGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCATGTTTCGCGGCGTCTTCCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggcca                                                                                                                                           <  1:1316334/150‑6 (MQ=255)
           tGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAGCGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccat                                                                                                                                          >  1:3201104/1‑144 (MQ=255)
              tAATGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTAAATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTAc                                                                                                                                       <  1:1417221/150‑1 (MQ=255)
              tAATATTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgc                                                                                                                                       <  1:1353107/150‑10 (MQ=255)
               aaTGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgcg                                                                                                                                      <  1:347617/150‑11 (MQ=255)
                 tgttgtTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgcgcc                                                                                                                                    <  1:771814/150‑13 (MQ=255)
                  gTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgcgcca                                                                                                                                   >  1:1287692/1‑137 (MQ=255)
                  gTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATAATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTggtggccattgcgccg                                                                                                                                   >  1:1496188/1‑136 (MQ=255)
                     aTTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCt                                                                                                                                <  1:3058180/150‑1 (MQ=255)
                        ttCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTa                                                                                                                             >  1:2429309/1‑150 (MQ=255)
                         tCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCATGTTTCGCGGCGTCTTCCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTAt                                                                                                                            <  1:1559190/150‑1 (MQ=255)
                          cAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTATc                                                                                                                           >  1:1232632/1‑150 (MQ=255)
                             aTGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTATCCAc                                                                                                                        <  1:3142508/150‑1 (MQ=255)
                               ggCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCActt                                                                                                                      >  1:2904335/1‑148 (MQ=255)
                                gCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCActtc                                                                                                                     <  1:629651/150‑4 (MQ=255)
                                gCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCActtc                                                                                                                     >  1:3257633/1‑147 (MQ=255)
                                 cTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCActtcc                                                                                                                    <  1:2875085/150‑5 (MQ=255)
                                 cTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCActtcc                                                                                                                    >  1:962198/1‑146 (MQ=255)
                                 cTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACACCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCActtcc                                                                                                                    >  1:1463587/1‑146 (MQ=255)
                                    aggctctGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCActtccact                                                                                                                 <  1:358740/144‑8 (MQ=255)
                                    aggctctGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCActtccac                                                                                                                  >  1:378521/7‑143 (MQ=255)
                                      gctctGGCAGAACGAAGGGCATGTTTCGCGGCGTCTTCCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTATCCActtccactca                                                                                                               >  1:3317123/5‑141 (MQ=255)
                                      aCTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGactac                                                                                                         <  1:2523161/150‑1 (MQ=255)
                                       ctctGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCActtccactcag                                                                                                              >  1:2793051/4‑140 (MQ=255)
                                       ctctGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCActtccactcag                                                                                                              >  1:941019/4‑140 (MQ=255)
                                        tctGGCAGAACGAAGGGCATGTTTCGCGGCGTCTTCCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTATCCActtccactcaga                                                                                                             >  1:1002077/3‑139 (MQ=255)
                                         ctGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGGTGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCActtccactcagac                                                                                                            >  1:574313/2‑138 (MQ=255)
                                           ggCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCActtccactcagacca                                                                                                          <  1:1193198/150‑15 (MQ=255)
                                            gCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAg                                                                                                   <  1:2717472/150‑1 (MQ=255)
                                                        gCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTg                                                                                       <  1:1531658/150‑1 (MQ=255)
                                                           tataTCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGgctgct                                                                                                                                            >  1:1216775/1‑100 (MQ=255)
                                                            atatCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAg                                                                                   <  1:3378486/150‑1 (MQ=255)
                                                                   ggcgTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGt                                                                            <  1:3339197/150‑1 (MQ=255)
                                                                    gcgTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGtt                                                                           <  1:1813680/150‑1 (MQ=255)
                                                                       tCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtgga                                                                              <  1:2643926‑M1/150‑43 (MQ=255)
                                                                        cTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGt                                                                       <  1:161953/150‑1 (MQ=255)
                                                                            cGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattcc                                                                         >  1:844080‑M1/1‑103 (MQ=255)
                                                                            cGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattcc                                                                         >  1:1396129‑M1/1‑103 (MQ=255)
                                                                            cGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattcc                                                                         >  1:176236‑M1/1‑103 (MQ=255)
                                                                            cGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAAATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACg                                                                   <  1:2567487/150‑1 (MQ=255)
                                                                            cGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattcc                                                                         >  1:34176‑M1/1‑103 (MQ=255)
                                                                             ggTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccg                                                                        <  1:2685459‑M1/150‑49 (MQ=255)
                                                                             ggTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccg                                                                        <  1:2351410‑M1/150‑49 (MQ=255)
                                                                                aacgacctctTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTATCCACttccactcagaccagaatcagtgccagttcctgcaaggtggaattccgtca                                                                     <  1:2093209‑M1/146‑52 (MQ=255)
                                                                                aacgacctctTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtca                                                                     <  1:1940081‑M1/146‑52 (MQ=255)
                                                                                aacaacCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtca                                                                     <  1:2261638‑M1/150‑52 (MQ=255)
                                                                                 acgacCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACTTCCACTCAGACCAGAGTCAGTGCCAGTTCCTGCAAGGCGGAATTCCGTCAc                                                                    >  1:283133/4‑150 (MQ=255)
                                                                                  cgacctCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacg                                                                   >  1:943934‑M1/3‑97 (MQ=255)
                                                                                  cgacCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCCTTATCCACTTCCACTCAGACCAGAGTCAGTGCCAGTTCCTGCAAGGTGGAATTCCGTCACg                                                                   >  1:465893/3‑150 (MQ=255)
                                                                                    acCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGaa                                                                             >  1:2622868/1‑131 (MQ=255)
                                                                                     ccTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggta                                                                >  1:1876843‑M1/1‑94 (MQ=255)
                                                                                      ctctTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcaaaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaa                                                               >  1:1260549‑M1/1‑93 (MQ=255)
                                                                                           ctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatga                                                          >  1:3737473‑M1/1‑88 (MQ=255)
                                                                                            tGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGa                                                   >  1:424541/1‑150 (MQ=255)
                                                                                            tGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgac                                                         >  1:369001‑M1/1‑87 (MQ=255)
                                                                                             gACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacg                                                        <  1:2503895‑M1/150‑65 (MQ=255)
                                                                                              acacTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgc                                                       <  1:2776050‑M1/150‑66 (MQ=255)
                                                                                              acacTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccacccagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgc                                                       <  1:2405048‑M1/150‑66 (MQ=255)
                                                                                               cacTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgct                                                      >  1:2752399‑M1/1‑84 (MQ=255)
                                                                                                acTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAAt                                               >  1:3278758/1‑150 (MQ=255)
                                                                                                 cTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctgg                                                    >  1:925974‑M1/1‑82 (MQ=255)
                                                                                                  tGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcatgcaaggtggaattccgtcacggtaacatgacgctgga                                                   >  1:648864‑M1/1‑81 (MQ=255)
                                                                                                   ggCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaa                                                  <  1:169272‑M1/150‑71 (MQ=255)
                                                                                                    gCGTTGAATTATTACCTATAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACTTCCACTCAGACCAGAGTCAGTGCCAGTTCCTGCAAGGTGGAATTCCGTCACGGTAACATGACggaattcc                                                 <  1:2321279/150‑8 (MQ=255)
                                                                                                     cGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTACCAGCTCCTg                                                                                       <  1:3172415/105‑1 (MQ=255)
                                                                                                      gTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaat                                               <  1:2033210‑M1/150‑74 (MQ=255)
                                                                                                      gTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaat                                               <  1:2376432‑M1/150‑74 (MQ=255)
                                                                                                      gTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaat                                               >  1:3622635‑M1/1‑77 (MQ=255)
                                                                                                      gTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaat                                               >  1:2004783‑M1/1‑77 (MQ=255)
                                                                                                      gTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaat                                               <  1:2458360‑M1/150‑74 (MQ=255)
                                                                                                       ttGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatc                                              <  1:1025122‑M1/150‑75 (MQ=255)
                                                                                                         gAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatcca                                            <  1:2120632‑M1/150‑77 (MQ=255)
                                                                                                           attattACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatc                                          >  1:527415‑M1/1‑72 (MQ=255)
                                                                                                            ttattaCCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagctcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcg                                         <  1:2968037‑M1/150‑80 (MQ=255)
                                                                                                             tattaCCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAgc                                  <  1:299864/150‑1 (MQ=255)
                                                                                                             tattaCCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctgg                                                    <  1:3832191‑M1/138‑69 (MQ=255)
                                                                                                                taCCTGTAGAAATAACGCCGGATGAGCAGAAAGGACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccag                                     >  1:310973‑M1/1‑67 (MQ=255)
                                                                                                                taCCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccag                                     <  1:2237387‑M1/150‑84 (MQ=255)
                                                                                                                    tgtgGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAAt                                               <  1:2550018/130‑1 (MQ=255)
                                                                                                                    tgtagaaataacgccGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagct                                 >  1:2058399‑M1/8‑63 (MQ=255)
                                                                                                                    tgtagaaataacgccGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagct                                 >  1:669765‑M1/8‑63 (MQ=255)
                                                                                                                        gaaataaCGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctc                             >  1:3650360‑M1/4‑59 (MQ=255)
                                                                                                                             aaCGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggt                        <  1:1083169‑M1/150‑97 (MQ=255)
                                                                                                                               cGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGTGTTGATcc                >  1:1145603/1‑150 (MQ=255)
                                                                                                                               cGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacgatgt                      >  1:3075336‑M1/1‑52 (MQ=255)
                                                                                                                                gCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgtt                     >  1:876221‑M1/1‑51 (MQ=255)
                                                                                                                                  cGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgttga                   >  1:587969‑M1/1‑49 (MQ=255)
                                                                                                                                     aTGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtctgtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgttgatcc                <  1:2204644‑M1/150‑105 (MQ=255)
                                                                                                                                     aTGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgttgatcc                >  1:3542696‑M1/1‑46 (MQ=255)
                                                                                                                                         gcagaaaGAACCTGTGGCGGCCATTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgttgatccgtga            >  1:3525503‑M1/4‑42 (MQ=255)
                                                                                                                                          ccGAAAGAACCGGTGGCTGTTCTTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCGCGGTAACATGACGCTGGAAAATCCTTCACCagagttg                                >  1:795136/1‑120 (MQ=255)
                                                                                                                                          cagaaAGAACCTGTGGCGGCCATTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgttgatccgtgaa           <  1:3025927‑M1/148‑110 (MQ=255)
                                                                                                                                             aaaGAACCGGTGGCTGCTCTTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGTGTTGATCCGCGAACTGAGTggg  <  1:1753917/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
AATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGTGTTGATCCGCGAACTGAGTGGG  >  Xen14_126/1904‑2194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑