BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	Xen14_126	2,043	C→A	P86Q (CCG→CAG)	Xen14_04726 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Xen14_126	2,043	0	C	A	81.4%	2194.1 / inf	964	P86Q (CCG→CAG)	Xen14_04726	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (70/109); new base A (381/402); total (451/513)
Fisher's exact test for biased strand distribution p-value = 2.48e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 977 total aligned reads displayed.

TGCTCGAGAAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTT‑‑‑‑A‑‑TCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGTGTTGATCCGCGAACTGAG  >  Xen14_126/1894‑2190

   tCGAGAAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGaa                                                                                                                                                              <  1:3822658/150‑1 (MQ=255)

    cgtgaAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGaaa                                                                                                                                                             <  1:2355808/147‑1 (MQ=255)

    cgtgaAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGaaa                                                                                                                                                             >  1:1288208/4‑150 (MQ=255)

     gtgaAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAg                                                                                                                                                            >  1:1928095/3‑150 (MQ=255)

     gtgaAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTCCTGACACTGGCGTTGAATTATTACTTGTAGAAATAACGCCGGATGAGCAGAAAg                                                                                                                                                            >  1:3183903/3‑150 (MQ=255)

      tgaAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGACGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAAAGCCGGATGAGCAGAAAGa                                                                                                                                                           >  1:3737432/2‑150 (MQ=255)

      tgaAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGa                                                                                                                                                           <  1:3588159/149‑1 (MQ=255)

      tgaAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGa                                                                                                                                                           <  1:1000276/149‑1 (MQ=255)

       gaAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCagaaagaa                                                                                                                                                          <  1:1367231/150‑1 (MQ=255)

        aaaaTGGCGTCAATGATGATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCAGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAAc                                                                                                                                                         <  1:1870918/150‑1 (MQ=255)

         aaaTGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAAcc                                                                                                                                                        <  1:2632680/150‑1 (MQ=255)

         aaaTGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAAcc                                                                                                                                                        <  1:267081/150‑1 (MQ=255)

          aaTGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCg                                                                                                                                                       >  1:71531/1‑150 (MQ=255)

               cGTCAATGATAATGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAATAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGgc                                                                                                                                                  <  1:3492823/150‑1 (MQ=255)

                gTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCATGTTTCGCGGCGTCTTCCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcg                                                                                                                                                 <  1:939154/150‑2 (MQ=255)

                 tCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcgg                                                                                                                                                <  1:2337720/150‑3 (MQ=255)

                  cAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggc                                                                                                                                               >  1:607094/1‑147 (MQ=255)

                   aaTGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAGTAACGCCGGATGAGCAGAAAGAACCTGTGgcggcc                                                                                                                                              <  1:3161803/150‑5 (MQ=255)

                    aTGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCATGTTTCGCGGCGTCTTCCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggcca                                                                                                                                             <  1:1316335/150‑6 (MQ=255)

                          aTGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgcgc                                                                                                                                       <  1:1555276/150‑12 (MQ=255)

                          aTGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgcgc                                                                                                                                       <  1:1520401/150‑12 (MQ=255)

                           tGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgcgcc                                                                                                                                      <  1:2765975/150‑13 (MQ=255)

                              tATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgcgccatc                                                                                                                                   >  1:3043407/1‑135 (MQ=255)

                                ttttCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCtt                                                                                                                                 >  1:2587634/1‑150 (MQ=255)

                                        tGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTT‑‑‑‑A‑‑TCCAc‑‑‑‑‑c                                                                                                              <  1:1829864/150‑1 (MQ=255)

                                          gCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCActtc                                                                                                                 <  1:629651/150‑4 (MQ=255)

                                          gCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCActtc                                                                                                                 >  1:1606235/1‑147 (MQ=255)

                                                gctctGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCActtccactca                                                                                                           <  1:2142513/146‑10 (MQ=255)

                                                 ctctGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCActtccactcag                                                                                                          >  1:1045367/4‑140 (MQ=255)

                                                   ctGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGGTGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCActtccactcagac                                                                                                        >  1:574313/2‑138 (MQ=255)

                                                   ctGGCAGAACGAAGGGCATGTTTCGCGGCGTCTTCCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTT‑‑‑‑A‑‑TCCActtccactcagac                                                                                                        <  1:3223667/149‑13 (MQ=255)

                                                     ggCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCActtccactcagacca                                                                                                      <  1:1193198/150‑15 (MQ=255)

                                                     ggCAGAACGAAGGGCATGTTTCGCGGCGTCTTCCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTT‑‑‑‑A‑‑TCCActtccactcagacca                                                                                                      >  1:273552/1‑136 (MQ=255)

                                                       cagaaCGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggcc                                                                                                                                              <  1:1571818/111‑5 (MQ=255)

                                                                            cggcgTCTTCCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaag                                                                               <  1:286612‑M1/150‑38 (MQ=255)

                                                                               cgTCTTCCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtg                                                                            <  1:2522562‑M1/150‑41 (MQ=255)

                                                                                      cGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattcc                                                                     >  1:1396131‑M1/1‑103 (MQ=255)

                                                                                       ggTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTT‑‑‑‑A‑‑TCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACgg                                                              >  1:961158/1‑150 (MQ=255)

                                                                                        gTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgt                                                                   >  1:1972222‑M1/1‑101 (MQ=255)

                                                                                        gTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTT‑‑‑‑A‑‑TCCAC‑‑‑‑‑C‑TAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGt                                                             >  1:1420846/1‑150 (MQ=255)

                                                                                         tAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGCAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCACTTCCACTCAGACCAGAGTCAGTGCCAGTTCCTGCAAGGTGGAATTCCGTc                                                                  <  1:3782074/150‑1 (MQ=255)

                                                                                          aacgacctctTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtca                                                                 <  1:2093206‑M1/146‑52 (MQ=255)

                                                                                           acgacctcTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcac                                                                >  1:1634113‑M1/4‑98 (MQ=255)

                                                                                           acaacCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTT‑‑‑‑A‑‑TCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAAc                                                          >  1:2106383/1‑150 (MQ=255)

                                                                                            cgacctCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacg                                                               >  1:943928‑M1/3‑97 (MQ=255)

                                                                                             gacCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTGTCCACTTCCAC‑‑‑‑‑T‑CAGACCAGAGTCAGTGCCAGTTCCTGCAAGGTGGAATTCCGTCACgg                                                              >  1:3460807/2‑150 (MQ=255)

                                                                                              acCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggt                                                             >  1:3121028‑M1/1‑95 (MQ=255)

                                                                                                 tcttctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaac                                                          >  1:2210953‑M1/1‑92 (MQ=255)

                                                                                                 tcttctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaac                                                          <  1:3565145‑M1/150‑59 (MQ=255)

                                                                                                  cttctGACACTGGCGTTGAATTATTACCTTTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaaca                                                         <  1:1235215‑M1/150‑60 (MQ=255)

                                                                                                  cttctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCACttccacttagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaaca                                                         >  1:408395‑M1/1‑91 (MQ=255)

                                                                                                  cttctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaaca                                                         >  1:2245199‑M1/1‑91 (MQ=255)

                                                                                                     ctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatga                                                      >  1:2818214‑M1/1‑88 (MQ=255)

                                                                                                        acacTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgc                                                   <  1:513749‑M1/150‑66 (MQ=255)

                                                                                                          acTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGTTCTTACTCCGTCTTT‑‑‑‑A‑‑TCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAAt                                           <  1:3284560/150‑1 (MQ=255)

                                                                                                           cTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtaccagttcctgcaaggtggaattccgtcacggtaacatgacgctgg                                                <  1:33507‑M1/150‑69 (MQ=255)

                                                                                                            tGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcatgcaaggtggaattccgtcacggtaacatgacgctgga                                               >  1:648864‑M1/1‑81 (MQ=255)

                                                                                                             ggCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaa                                              <  1:169267‑M1/150‑71 (MQ=255)

                                                                                                                gTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaat                                           <  1:2033209‑M1/150‑74 (MQ=255)

                                                                                                                gTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaat                                           <  1:2453653‑M1/150‑74 (MQ=255)

                                                                                                                gTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaat                                           <  1:1015446‑M1/150‑74 (MQ=255)

                                                                                                                 ttGAATTATTACCTGTAGAAATAACGCCGGATGGGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatc                                          >  1:2489120‑M1/1‑76 (MQ=255)

                                                                                                                 ttGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatc                                          <  1:2540516‑M1/150‑75 (MQ=255)

                                                                                                                  tGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatcc                                         <  1:1220697‑M1/150‑76 (MQ=255)

                                                                                                                  tGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatcc                                         >  1:3395299‑M1/1‑75 (MQ=255)

                                                                                                                   gAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTT‑‑‑‑A‑‑TCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCa                                  >  1:2950569/1‑150 (MQ=255)

                                                                                                                   gAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatcca                                        >  1:1725791‑M1/1‑74 (MQ=255)

                                                                                                                   gAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatcca                                        >  1:459864‑M1/1‑74 (MQ=255)

                                                                                                                     attattACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggagttccgtcacggtaacatgacgctggaaaatccttc                                      >  1:768687‑M1/1‑72 (MQ=255)

                                                                                                                     attattACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatc                                      >  1:3526269‑M1/1‑72 (MQ=255)

                                                                                                                         ttaCCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgcca                                  >  1:900080‑M1/1‑68 (MQ=255)

                                                                                                                          taCCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccag                                 <  1:2975244‑M1/150‑84 (MQ=255)

                                                                                                                            ccTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagag                               >  1:2277517‑M1/1‑65 (MQ=255)

                                                                                                                             ctgtagaaataacgccgGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagc                              <  1:2790902‑M1/142‑87 (MQ=255)

                                                                                                                             ctgtagaaataacgccgGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagc                              <  1:826399‑M1/142‑87 (MQ=255)

                                                                                                                             ctgtagaaataacgccgGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagc                              >  1:3269496‑M1/9‑64 (MQ=255)

                                                                                                                             ctgtagaaataacgccgGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagc                              >  1:3010719‑M1/9‑64 (MQ=255)

                                                                                                                              tgtgGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTT‑‑‑‑A‑‑TCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACAt                                                        <  1:2889701/117‑1 (MQ=255)

                                                                                                                              tgtagaaataacgccGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagct                             >  1:3652169‑M1/8‑63 (MQ=255)

                                                                                                                              tgtagaaataacgccGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagct                             >  1:2058399‑M1/8‑63 (MQ=255)

                                                                                                                              tgtagaaataacgccGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagct                             >  1:669765‑M1/8‑63 (MQ=255)

                                                                                                                              tgtagaaataacgccGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagct                             >  1:2697595‑M1/8‑63 (MQ=255)

                                                                                                                               gtagaaataacgcCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctg                            >  1:3629476‑M1/7‑62 (MQ=255)

                                                                                                                               gtagaaataacgcCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctg                            >  1:3074849‑M1/7‑62 (MQ=255)

                                                                                                                               gtagaaataacgcCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctg                            >  1:2566818‑M1/7‑62 (MQ=255)

                                                                                                                                tagaaataacgCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgc                           >  1:3325345‑M1/6‑61 (MQ=255)

                                                                                                                                    aatAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcac                       <  1:1768147‑M1/149‑94 (MQ=255)

                                                                                                                                      tAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacgg                     >  1:785424‑M1/1‑55 (MQ=255)

                                                                                                                                       aaCGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggt                    <  1:873688‑M1/150‑97 (MQ=255)

                                                                                                                                       aaCGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggt                    <  1:1083169‑M1/150‑97 (MQ=255)

                                                                                                                                         cGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTT‑‑‑‑A‑‑TCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGTGTTGATcc            >  1:1145603/1‑150 (MQ=255)

                                                                                                                                         cGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgt                  >  1:1262722‑M1/1‑52 (MQ=255)

                                                                                                                                          gCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgtt                 >  1:3690768‑M1/1‑51 (MQ=255)

                                                                                                                                           ccGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgttg                <  1:1997112‑M1/150‑101 (MQ=255)

                                                                                                                                            cGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagctcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgttga               >  1:587970‑M1/1‑49 (MQ=255)

                                                                                                                                              gATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgttgatc             <  1:1470393‑M1/150‑104 (MQ=255)

                                                                                                                                              gATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgttgatc             <  1:2381441‑M1/150‑104 (MQ=255)

                                                                                                                                              gATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgttgatc             <  1:3421041‑M1/150‑104 (MQ=255)

                                                                                                                                               aTGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTT‑‑‑‑A‑‑TCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCAGGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGTGTTGATCCGCGAAc      <  1:2409606/150‑1 (MQ=255)

                                                                                                                                                tGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgttgatccg           >  1:3095657‑M1/1‑45 (MQ=255)

TGCTCGAGAAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTT‑‑‑‑A‑‑TCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGTGTTGATCCGCGAACTGAG  >  Xen14_126/1894‑2190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`