BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	Xen14_126	2,026	G→A	E80E (GAG→GAA)	Xen14_04726 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Xen14_126	2,026	0	G	A	81.7%	1817.2 / inf	991	E80E (GAG→GAA)	Xen14_04726	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (69/112); new base A (394/414); total (464/527)
Fisher's exact test for biased strand distribution p-value = 1.06e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

Only 100 of 997 total aligned reads displayed.

CTTGTGTGGCCCAGATTGCTCGAGAAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGTGTT  >  Xen14_126/1878‑2175

    tgttgcacAGATTGCACGTGAAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAAc                                                                                                                                                        >  1:1742926/8‑150 (MQ=255)

    tgttgcacAGATTGCACGTGAAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAAc                                                                                                                                                        >  1:335075/8‑150 (MQ=255)

     gttgcacAGATTGCACGTGAAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACg                                                                                                                                                       >  1:123685/7‑150 (MQ=255)

       tgcacAGATTGCACGTGAAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGTGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGcc                                                                                                                                                     <  1:1175613/146‑1 (MQ=255)

        acacAGATTGCACGAGAAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCATGTTTCGCGGCGTCTTCCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCg                                                                                                                                                    <  1:2145823/147‑1 (MQ=255)

        acacAGATTGCACGAGAAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCATGTTTCGCGGCGTCTTCCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCg                                                                                                                                                    <  1:3732165/147‑1 (MQ=255)

         cccAGATTGCTCGAGAAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCgg                                                                                                                                                   <  1:2975829/150‑1 (MQ=255)

          acAGATTGCACGTGAAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGTGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGa                                                                                                                                                  <  1:3270869/149‑1 (MQ=255)

           cAGATTGCACGTGAAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGAt                                                                                                                                                 <  1:782419/150‑1 (MQ=255)

           cAGATTGCACGTGAAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGCAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGAt                                                                                                                                                 <  1:2582041/150‑1 (MQ=255)

             gATTGCTCGAGAAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGa                                                                                                                                               <  1:443777/150‑1 (MQ=255)

              aTTGCACGTGAAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGTGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAg                                                                                                                                              <  1:271306/150‑1 (MQ=255)

                tgcacgtgaAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGca                                                                                                                                            <  1:2294122/143‑2 (MQ=255)

                 gcacgtgaAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGcag                                                                                                                                           <  1:259468/144‑3 (MQ=255)

                 gcacgtgaAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGcag                                                                                                                                           <  1:1999745/144‑3 (MQ=255)

                  cTCGAGAAAATGGCGTCAATGATAATGTTATTTTCAAATGGCCCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGa                                                                                                                                          >  1:646020/1‑150 (MQ=255)

                   acgtgaAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGaa                                                                                                                                         >  1:2103283/5‑150 (MQ=255)

                    cgtgaAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGTGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCAGGATGAGCAGaaa                                                                                                                                        >  1:3767427/4‑150 (MQ=255)

                      tgaAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGa                                                                                                                                      <  1:1000276/149‑1 (MQ=255)

                      agaAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGATACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGa                                                                                                                                      >  1:3088955/1‑150 (MQ=255)

                        aaaaTGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGTGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAAc                                                                                                                                    <  1:351362/150‑1 (MQ=255)

                        aaaaTGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGATACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAAc                                                                                                                                    >  1:2939161/1‑150 (MQ=255)

                         aaaTGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAAcc                                                                                                                                   <  1:3633975/150‑1 (MQ=255)

                         aaaTGGCGTCAATGATAATATTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACGCTGGCGTTGAATTATTACCTGTAGAAATAGCGCCGGATGAGCAGAAAGAAcc                                                                                                                                   >  1:3799648/1‑150 (MQ=255)

                          aaTGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCg                                                                                                                                  >  1:71531/1‑150 (MQ=255)

                           aTGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAAcctg                                                                                                                                 >  1:3168183/1‑148 (MQ=255)

                             ggCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCtgtg                                                                                                                               <  1:1521378/150‑1 (MQ=255)

                                 tCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcgg                                                                                                                           <  1:2228953/150‑3 (MQ=255)

                                 tCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcgg                                                                                                                           <  1:2641052/150‑3 (MQ=255)

                                  cAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCATGTTTCGCGGCGTCTTCTGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggc                                                                                                                          <  1:1949170/150‑4 (MQ=255)

                                   aaTGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCATGTTTCGCGGCGTCTTCCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggcc                                                                                                                         <  1:825549/150‑5 (MQ=255)

                                    aTGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAg                                                                                                                                              <  1:1596576/128‑1 (MQ=255)

                                     taaTAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccat                                                                                                                       >  1:2899934/3‑144 (MQ=255)

                                     tGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAGCGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccat                                                                                                                       >  1:3201104/1‑144 (MQ=255)

                                     tGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGTGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccat                                                                                                                       >  1:1265358/1‑144 (MQ=255)

                                     tGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCATGTTTCGCGGCGTCTTCCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCCGTGgcggccat                                                                                                                       >  1:2445109/1‑144 (MQ=255)

                                       aTAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCATGTTTCGCGGCGTCTTCCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattg                                                                                                                     <  1:3031797/150‑9 (MQ=255)

                                        tAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgc                                                                                                                    <  1:620201/150‑10 (MQ=255)

                                          aTGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgcgc                                                                                                                  <  1:1555276/150‑12 (MQ=255)

                                          aTGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgcgc                                                                                                                  <  1:1890234/150‑12 (MQ=255)

                                           tGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgcgcc                                                                                                                 <  1:2092401/150‑13 (MQ=255)

                                           tGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCATGTTTCGCGGTGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgcgcc                                                                                                                 >  1:1473473/1‑138 (MQ=255)

                                            gTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCATGTTTCGCGGCGTCTTCCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgcgccg                                                                                                                <  1:3066698/150‑14 (MQ=255)

                                              tATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGTGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgcgccatc                                                                                                              >  1:2375423/1‑135 (MQ=255)

                                                        tGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTCCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCAct                                                                                                    <  1:1865555/150‑2 (MQ=255)

                                                        tGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTATCCAc‑‑‑‑‑c                                                                                               <  1:2770572/150‑1 (MQ=255)

                                                         ggCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTATCCActt                                                                                                   <  1:1063653/150‑3 (MQ=255)

                                                          gCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGTGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCActtc                                                                                                  <  1:933397/150‑4 (MQ=255)

                                                          gCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCActtc                                                                                                  >  1:2553208/1‑147 (MQ=255)

                                                                 ctctGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCActtccactcag                                                                                           >  1:2678890/4‑140 (MQ=255)

                                                                 ctctGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCActtccactcag                                                                                           <  1:3592377/147‑11 (MQ=255)

                                                                 ctctGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCActtccactcag                                                                                           >  1:2793051/4‑140 (MQ=255)

                                                                  tttGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTg                                                                                    <  1:3289141/150‑1 (MQ=255)

                                                                  tctGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCActtccactcaga                                                                                          >  1:3321122/3‑139 (MQ=255)

                                                                    tGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAg                                                                                           <  1:1640913/141‑1 (MQ=255)

                                                                            ggaccggcgtggcgcgcgGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAg                                                                          <  1:1295744/136‑1 (MQ=255)

                                                                            cGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAg                                                                          <  1:56702/150‑1 (MQ=255)

                                                                              aaGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCt                                                                        <  1:287907/150‑1 (MQ=255)

                                                                                      atatCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAg                                                                <  1:3378486/150‑1 (MQ=255)

                                                                                            cggcgTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaag                                                                <  1:2042824‑M1/150‑38 (MQ=255)

                                                                                               cgTCTTCCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtg                                                             <  1:2534006‑M1/150‑41 (MQ=255)

                                                                                                   ttccggtAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaat                                                         >  1:1202360‑M1/4‑106 (MQ=255)

                                                                                                      cGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattcc                                                      >  1:34176‑M1/1‑103 (MQ=255)

                                                                                                       ggTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccg                                                     <  1:169335‑M1/150‑49 (MQ=255)

                                                                                                       ggTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccg                                                     >  1:3835456‑M1/1‑102 (MQ=255)

                                                                                                       ggTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccg                                                     <  1:595673‑M1/150‑49 (MQ=255)

                                                                                                       ggTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACgg                                               >  1:961157/1‑150 (MQ=255)

                                                                                                        gTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgt                                                    >  1:2476360‑M1/1‑101 (MQ=255)

                                                                                                         tAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtc                                                   <  1:66415‑M1/150‑51 (MQ=255)

                                                                                                            cgacctCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacg                                                >  1:943927‑M1/3‑97 (MQ=255)

                                                                                                            cgacCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATAAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACTTCCACTCAGACCAGAGTCAGTGCCAGTTCCTGCAAGGTGGAATTCCGTCACg                                                >  1:3457439/3‑150 (MQ=255)

                                                                                                            caacCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCATAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTATCCACctccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacg                                                >  1:3677322‑M1/1‑97 (MQ=255)

                                                                                                             gaccTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacgg                                               <  1:1322776‑M1/149‑55 (MQ=255)

                                                                                                             gaccTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacgg                                               >  1:2327636‑M1/2‑96 (MQ=255)

                                                                                                              acCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggt                                              >  1:3121028‑M1/1‑95 (MQ=255)

                                                                                                              acCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggt                                              >  1:1229721‑M1/1‑95 (MQ=255)

                                                                                                               ccTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggacttccgtcacggta                                             >  1:1375408‑M1/1‑94 (MQ=255)

                                                                                                               ccTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggta                                             >  1:3472967‑M1/1‑94 (MQ=255)

                                                                                                                 tcttctGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACg                                     <  1:3234784/150‑1 (MQ=255)

                                                                                                                 tcttctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaac                                           >  1:142519‑M1/1‑92 (MQ=255)

                                                                                                                 tcttctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaac                                           <  1:1570501‑M1/150‑59 (MQ=255)

                                                                                                                   ttctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacat                                         <  1:870390‑M1/150‑61 (MQ=255)

                                                                                                                    tntGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatg                                        >  1:3561025‑M1/1‑89 (MQ=255)

                                                                                                                    tctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatg                                        >  1:2353464‑M1/1‑89 (MQ=255)

                                                                                                                      tGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGa                                >  1:424541/1‑150 (MQ=255)

                                                                                                                        acacTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgc                                    <  1:2405049‑M1/150‑66 (MQ=255)

                                                                                                                          acTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAAt                            <  1:1400221/150‑1 (MQ=255)

                                                                                                                           cTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATc                           <  1:1801404/150‑1 (MQ=255)

                                                                                                                           cTGGCGGTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACTTCCACTCAGACCAGAGTCAGTGCCAGTTCCTGAAAGGTGGAATTCCGTCACGGTAACATGACGCTgg                                 <  1:225401/150‑1 (MQ=255)

                                                                                                                                gTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaat                            <  1:2033208‑M1/150‑74 (MQ=255)

                                                                                                                                gTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaat                            <  1:1015446‑M1/150‑74 (MQ=255)

                                                                                                                                gTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaat                            <  1:2854186‑M1/150‑74 (MQ=255)

                                                                                                                                  tGAATTATTACCTGTAGAAATAACGCCGGATGAGCGGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTGTCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatcc                          <  1:488624‑M1/150‑76 (MQ=255)

                                                                                                                                     attattACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggagttccgtcacggtaacatgacgctggaaaatccttc                       >  1:768687‑M1/1‑72 (MQ=255)

                                                                                                                                     attattACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatc                       >  1:2503909‑M1/1‑72 (MQ=255)

                                                                                                                                       tattaCCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAgc               <  1:299866/150‑1 (MQ=255)

                                                                                                                                       tattaCCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATTCACTTCCGCTCAGACCAGAGTCAGTGCCAGTTCCTGCAAGGTGGAATTCCGTCACGGTAACATGACGCTGGAAAATccatcgc                     <  1:1101474/150‑6 (MQ=255)

                                                                                                                                        attaCCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgcc                    >  1:2743003‑M1/1‑69 (MQ=255)

                                                                                                                                         ttaCCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgcca                   >  1:900078‑M1/1‑68 (MQ=255)

                                                                                                                                          taCCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAgctgc            <  1:1982143/150‑1 (MQ=255)

CTTGTGTGGCCCAGATTGCTCGAGAAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGTGTT  >  Xen14_126/1878‑2175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`