BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	Xen14_126	2,023	G→A	V79V (GTG→GTA)	Xen14_04726 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Xen14_126	2,023	0	G	A	82.2%	1839.1 / 305.9	983	V79V (GTG→GTA)	Xen14_04726	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (68/107); new base A (395/412); total (464/519)
Fisher's exact test for biased strand distribution p-value = 1.56e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 992 total aligned reads displayed.

CTTGTGTGGCCCAGATTGCTCGAGAAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGT  >  Xen14_126/1878‑2172

   gtgttgcacAGATTGCACGTGAAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATaa                                                                                                                                                      >  1:321283/9‑150 (MQ=255)

     gttgcacAGATTGCACGTGAAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACg                                                                                                                                                    >  1:123685/7‑150 (MQ=255)

       tgcacAGATTGCACGTGAAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGTGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGcc                                                                                                                                                  <  1:1175613/146‑1 (MQ=255)

       tgcacAGATTGCACGTGAAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGcc                                                                                                                                                  >  1:2184640/5‑150 (MQ=255)

       tgcacAGATTGCACGTGAAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGcc                                                                                                                                                  <  1:2806915/146‑1 (MQ=255)

         cccAGATTGCTCGAGAAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCgg                                                                                                                                                <  1:2975832/150‑1 (MQ=255)

           cAGATTGCACGTGAAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGAt                                                                                                                                              <  1:3520648/150‑1 (MQ=255)

           cAGATTGCACGTGAAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGAt                                                                                                                                              <  1:3237051/150‑1 (MQ=255)

           cAGATTGCACGTGAAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGAt                                                                                                                                              <  1:782419/150‑1 (MQ=255)

               ttGCACGAGAAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCATGTTTCGCGGCGTCTTCCGGTAACAACCTCTTCCGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGc                                                                                                                                          <  1:1965087/150‑1 (MQ=255)

                tgcacgtgaAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTGCCTGTAGAAATAACGCCGGATGAGca                                                                                                                                         <  1:2387606/143‑2 (MQ=255)

                  cTCGAGAAAATGGCGTCAATGATAATGTTATTTTCAAATGGCCCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGa                                                                                                                                       >  1:646020/1‑150 (MQ=255)

                    cgtgaAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGaaa                                                                                                                                     >  1:3301451/4‑150 (MQ=255)

                     gtgaAAATGGCGTCAATNATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCAGGATGAGCAGAAAg                                                                                                                                    >  1:3569938/3‑150 (MQ=255)

                     gtgaAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACCCTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAgtagaaag                                                                                                                                    >  1:3354741/3‑143 (MQ=255)

                     gtgaAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAg                                                                                                                                    >  1:1928095/3‑150 (MQ=255)

                     gtgaAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAg                                                                                                                                    >  1:2725770/3‑150 (MQ=255)

                     gtgaAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAg                                                                                                                                    >  1:383758/3‑150 (MQ=255)

                      tgaAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGa                                                                                                                                   <  1:1000276/149‑1 (MQ=255)

                        aaaaTGGCGTCAATGATGATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCAGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAAc                                                                                                                                 <  1:1870918/150‑1 (MQ=255)

                          aaTGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCg                                                                                                                               >  1:71531/1‑150 (MQ=255)

                             ggCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCtgtg                                                                                                                            <  1:513082/150‑1 (MQ=255)

                                 tCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcgg                                                                                                                        <  1:2337720/150‑3 (MQ=255)

                                 tCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGACGAGCAGAAAGAACCTGTGgcgg                                                                                                                        <  1:1989015/150‑3 (MQ=255)

                                  cAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggc                                                                                                                       <  1:2538364/150‑4 (MQ=255)

                                   aaTGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCATGTTTCGCGGCGTCTTCCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggcc                                                                                                                      <  1:825549/150‑5 (MQ=255)

                                   aaTGATAATGTTATTTTCAAATGGCTCAGGCTCTGACAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTCGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAAACTGTGgcggcc                                                                                                                      >  1:3774867/1‑146 (MQ=255)

                                     tGATAATGTTCTTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccat                                                                                                                    >  1:359496/1‑144 (MQ=255)

                                     tGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGTGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccat                                                                                                                    >  1:1265358/1‑144 (MQ=255)

                                     tGATAATGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGTGTTGAATTATTACCGGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGctct                                                                                                                    <  1:2583760/150‑1 (MQ=255)

                                        tAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgc                                                                                                                 <  1:620201/150‑10 (MQ=255)

                                         aaTGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgcg                                                                                                                <  1:347617/150‑11 (MQ=255)

                                          aTGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgcgc                                                                                                               <  1:3273348/150‑12 (MQ=255)

                                            gTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGTGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTAGCGTTGAATTATTACTTGTAGAAATAGCGCCGGATGAGCAGAAAGAACCTGTGgcggccattgcgccg                                                                                                             >  1:3337053/1‑137 (MQ=255)

                                            gTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgcgcca                                                                                                             >  1:2883198/1‑137 (MQ=255)

                                            gTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCATGTTTCGCGGCGTCTTCCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgcgccg                                                                                                             <  1:3001691/150‑14 (MQ=255)

                                              tATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgcgccatc                                                                                                           >  1:2058154/1‑135 (MQ=255)

                                              tATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgcgccatc                                                                                                           <  1:2484373/150‑16 (MQ=255)

                                                  ttCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTa                                                                                                       <  1:596797/150‑1 (MQ=255)

                                                       aTGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCAc                                                                                                  <  1:1609373/150‑1 (MQ=255)

                                                         ggCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTATCCActt                                                                                                <  1:1063653/150‑3 (MQ=255)

                                                          gCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGTGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCActtc                                                                                               <  1:933397/150‑4 (MQ=255)

                                                           cTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGATAACGACCTCTTCTGACACTGGCGTTGAATTATTAACTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCActtcc                                                                                              >  1:2566270/1‑146 (MQ=255)

                                                            tcaggctctGGCAGAACGAAGGGCATGTTTCGCGGCGTCTTCCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTATCCActtcca                                                                                             >  1:1758217/9‑145 (MQ=255)

                                                            tCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGa                                                                                       >  1:363399/1‑150 (MQ=255)

                                                                 cTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTTGCGTTGAATTATTACCTGTGGAGATACCGCCGGATTAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGactact                                                                                  >  1:444292/1‑150 (MQ=255)

                                                                   ctGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGGTGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCActtccactcagac                                                                                      >  1:574312/2‑138 (MQ=255)

                                                                   ctGGCAGAACGAAGGGCATGTTTCGCGGCGTCTTCCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTATCCActtccactc                                                                                          <  1:2511550/145‑9 (MQ=255)

                                                                       cagaaCGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggcc                                                                                                                      <  1:1571818/111‑5 (MQ=255)

                                                                        agaaCGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAAcct                                                                                                                               <  1:2115531/102‑2 (MQ=255)

                                                                                          cgcgGCGTCTTCCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgca                                                               >  1:1555394‑M1/1‑115 (MQ=255)

                                                                                            cggcgTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAg                                                       <  1:3171541/150‑1 (MQ=255)

                                                                                              gcgTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGtt                                                     <  1:1813681/150‑1 (MQ=255)

                                                                                                  cttccggtaACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaa                                                       >  1:2414102‑M1/5‑107 (MQ=255)

                                                                                                   ttccggtAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaat                                                      >  1:1202360‑M1/4‑106 (MQ=255)

                                                                                                       ggTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccg                                                  <  1:2685459‑M1/150‑49 (MQ=255)

                                                                                                       ggTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACgg                                            >  1:961157/1‑150 (MQ=255)

                                                                                                       ggTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccg                                                  <  1:2956661‑M1/150‑49 (MQ=255)

                                                                                                         tAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtc                                                >  1:3734819‑M1/1‑100 (MQ=255)

                                                                                                         tAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtc                                                <  1:66415‑M1/150‑51 (MQ=255)

                                                                                                           acgacctcTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcac                                              >  1:3680515‑M1/4‑98 (MQ=255)

                                                                                                           acgacctcTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcac                                              <  1:1717698‑M1/147‑53 (MQ=255)

                                                                                                            cgacctCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacg                                             >  1:1333727‑M1/3‑97 (MQ=255)

                                                                                                            cgacctCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacg                                             >  1:943927‑M1/3‑97 (MQ=255)

                                                                                                             gaccTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacgg                                            >  1:1402975‑M1/2‑96 (MQ=255)

                                                                                                             gaccTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacgg                                            <  1:1677430‑M1/149‑55 (MQ=255)

                                                                                                              acCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggt                                           >  1:1229721‑M1/1‑95 (MQ=255)

                                                                                                               ccTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggta                                          >  1:2455070‑M1/1‑94 (MQ=255)

                                                                                                                 tcttctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaac                                        >  1:1300972‑M1/1‑92 (MQ=255)

                                                                                                                 tcttctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaac                                        >  1:2018576‑M1/1‑92 (MQ=255)

                                                                                                                  cttctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaaca                                       >  1:3461302‑M1/1‑91 (MQ=255)

                                                                                                                   ttctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacat                                      <  1:870390‑M1/150‑61 (MQ=255)

                                                                                                                     ctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatga                                    >  1:2644333‑M1/1‑88 (MQ=255)

                                                                                                                     ctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatga                                    >  1:3042342‑M1/1‑88 (MQ=255)

                                                                                                                      tGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGa                             >  1:424542/1‑150 (MQ=255)

                                                                                                                        acacTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgc                                 <  1:2776050‑M1/150‑66 (MQ=255)

                                                                                                                         cacTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgct                                >  1:2752399‑M1/1‑84 (MQ=255)

                                                                                                                           cTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctgg                              >  1:30716‑M1/1‑82 (MQ=255)

                                                                                                                           cTGGCGTTGAAGTATTACCTGTCGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACTTCCACTCAGACCAGAGTCAGTGCCAGTTCCTACAAGGTGGAATTCCGTCACGGTAACATGACGCTgg                              >  1:3095779/1‑150 (MQ=255)

                                                                                                                            tGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATcc                       <  1:3140194/150‑1 (MQ=255)

                                                                                                                             ggCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCt                      >  1:2093551/1‑150 (MQ=255)

                                                                                                                               cGTTGAATTATTGCCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTc                    <  1:2616134/150‑1 (MQ=255)

                                                                                                                                gTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaat                         <  1:2033215‑M1/150‑74 (MQ=255)

                                                                                                                                gTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaat                         <  1:1015446‑M1/150‑74 (MQ=255)

                                                                                                                                  tGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatcc                       >  1:3424238‑M1/1‑75 (MQ=255)

                                                                                                                                  tGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatcc                       <  1:2301957‑M1/150‑76 (MQ=255)

                                                                                                                                  tGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatcc                       >  1:3395299‑M1/1‑75 (MQ=255)

                                                                                                                                  tGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatcc                       >  1:1381065‑M1/1‑75 (MQ=255)

                                                                                                                                  tGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatcc                       <  1:3803690‑M1/150‑76 (MQ=255)

                                                                                                                                    aaTTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaccatgacgctggaaaatccat                     >  1:2262175‑M1/1‑73 (MQ=255)

                                                                                                                                     attattACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggagttccgtcacggtaacatgacgctggaaaatccttc                    >  1:768687‑M1/1‑72 (MQ=255)

                                                                                                                                     attattACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatc                    <  1:2908562‑M1/150‑79 (MQ=255)

                                                                                                                                     attattACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatc                    <  1:2856981‑M1/150‑79 (MQ=255)

                                                                                                                                     attattACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatc                    >  1:1524466‑M1/1‑72 (MQ=255)

                                                                                                                                       tattaCCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATTCACTTCCGCTCAGACCAGAGTCAGTGCCAGTTCCTGCAAGGTGGAATTCCGTCACGGTAACATGACGCTGGAAAATccatcgc                  <  1:1101474/150‑6 (MQ=255)

                                                                                                                                       tattaCCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgc                  >  1:1433198‑M1/1‑70 (MQ=255)

                                                                                                                                         ttaCCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgcca                >  1:900078‑M1/1‑68 (MQ=255)

                                                                                                                                          taCCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccag               <  1:2237387‑M1/150‑84 (MQ=255)

                                                                                                                                          taCCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccag               >  1:1486576‑M1/1‑67 (MQ=255)

                                                                                                                                              tgtgGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACAt                                      <  1:236842/117‑1 (MQ=255)

CTTGTGTGGCCCAGATTGCTCGAGAAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGT  >  Xen14_126/1878‑2172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`