Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Xen14_281320GC74.9% 400.2 / 156.0 212intergenic (–/‑453)–/gadC–/putative glutamate/gamma‑aminobutyrate antiporter
Reads supporting (aligned to +/- strand):  ref base G (33/20);  new base C (74/84);  total (108/104)
Fisher's exact test for biased strand distribution p-value = 5.80e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.43e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 212 total aligned reads displayed.

‑‑‑‑‑‑‑‑‑‑‑‑‑ATTGCACAGTACTATGAATTCCTGCGCCTCGGTCGTGAAGGCTATACCAAAGTACAGAACGCTTCCTACCAGGTTGCTGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACGGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTATGACCTCTCTGAACGTCTGCGTCTGCACGGCTGGCAGGTTCCGGCCTTCACTCTCGGCGGTGAAGCCACTG  >  Xen14_28/1‑277
                                                                                                                                                |                                                                                                                                                 
ggcgggtcaggtgaTTGCACAGTACTATGAATTCCTGCGCCTCGGTCGTGAAGGCTATACCAAAGTACAGAACGCTTCCTACCAGGTTGCCGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCg                                                                                                                                              <  1:2893604/137‑1 (MQ=18)
 gcgggtcaggtgaTTGCGCAGTACTATGAATTCCTGCGCCTCGGTCGTGAAGGCTATACCAAAGTACAGAACGCTTCCTACCAGGTTGCTGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACGGGTCGc                                                                                                                                             <  1:1867074/138‑1 (MQ=21)
       caggtgaTTGCACAGTACTATGAATTCCTGCGCCTCGGTCGTGAAGGCTATACCAAAGTACAGAACGCTTCCTACCAGGTTGCCGCTTATCTGGCGGATGAAATCGCCAAACCGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGAc                                                                                                                                       <  1:105419/144‑1 (MQ=21)
        aggtgaTTGCACAGTACTATGAATTCCTGCGCCTCGGTCGTGAAGGCTATACCAAAGTACAGAACGCTTCCTACCAGGTTGCTGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACGGGTCGCCCGGACg                                                                                                                                      >  1:1407953/6‑150 (MQ=255)
          gtgaTTGCACAGTACTATGAATTCCTGCGCCTCGGTCGTGAAGGCTATACCAAAGTACAGAACGCTTCCTACCAGGTTGCCGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGaa                                                                                                                                    <  1:602211/147‑1 (MQ=255)
           tgaTTGCACAGTACTATGAATTCCTGCGCCTCGGTCGTGAAGGTTATACCAAAGTACAGAACGCTTCCTACCAGGTTGCCGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAg                                                                                                                                   <  1:242923/148‑1 (MQ=255)
            gaTTGCACAGTGCTATGAATTCCTGCGCCTCGGTCGTGAAGGCTATACCAAAGTACAGAACGCTTCCTACCAGGTTGCCGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAgg                                                                                                                                  <  1:1214598/149‑1 (MQ=255)
            gaTTGCACAGTACTATGAATTCCTGCGCCTCGGTCGTGAAGGCTATACCAAAGTACAGAACGCTTCCTACCAGGTTGCTGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTGTGAGTTCATCTGTACGGGTCGCCCGGACGAAgg                                                                                                                                  <  1:2797849/149‑1 (MQ=255)
                        cTATGAATTCCTGCACCTCGGTCGTGAAGGCTATACCAAAGTACAGAACGCTTCCTACCAGGTTGCCGCTTATCTGGGGGATGAAATCACCAAACTGGGGCAGTATGAGTTCATCTGTACCGGTCGcacg                                                                                                                                          >  1:48938/1‑127 (MQ=21)
                         tATGAATTCCTGCGCCTCGGGCGTGAAGGCTATACCAAAGTACAGAACGCTTCCTACCAGGTTGCTGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACGGGTCGCCCGGACGAAGGCATCCCGGCGGtt                                                                                                                     <  1:3643126/150‑1 (MQ=255)
                             aaTTCCTGCGCCTCGGTCGTGAAGGCTATACCAAAGTACAGAACGCTTCCTACCAGGTTGCTGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACGGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCt                                                                                                                 <  1:1640879/150‑1 (MQ=255)
                              aTTCCTGCGCCTCGGTCGTGAAGGCTATACCAAAGTACAGAACGCTTCCTACCAGGTTGCCGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCtt                                                                                                                <  1:104169/150‑1 (MQ=255)
                               ttCCTGCGCCTCGGTCGTGAAGGCTATACCAAAGTACAGAACGCTTCCTACCAGGTTGCTGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACGGGCCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTc                                                                                                               >  1:1902900/1‑150 (MQ=255)
                               ttCCTGCGCCTCGGTCGTGAAGGCTATACCAAAGTACAGAACGCTTCCTACCAGGTTGCCGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTc                                                                                                               >  1:1801789/1‑150 (MQ=255)
                               ttCCTACGCCTCGGTCGGGAAGGCTATACCAAAGTAAAGAGCGTTTCCTACCAGGTTGCCGTTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTc                                                                                                               <  1:3617602/150‑1 (MQ=255)
                                tCCTGCGCCTCGGTCGTGAAGGCTATACCAAAGTACAGAACGCTTCCTACCAGGTTGCCGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCa                                                                                                              >  1:2310262/1‑150 (MQ=255)
                                tCCTGCGCCTCGGTCGTGAAGGCTATACCAAAGTACAGAACGCTTCCTACCAGGTTGCCGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCa                                                                                                              <  1:3043107/150‑1 (MQ=255)
                                   tGCGCCTCGGTCGTGAAGGCTATACCAAAGTACAGAACGCTTCCTACCAGGTTGCTGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACGGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAAc                                                                                                           <  1:1219911/150‑1 (MQ=255)
                                   tGCGCCTCGGTCGTGAAGGCTATACCAAAGTACAGAACGCTTCCTACCAGGTTGCCGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAAc                                                                                                           <  1:3225315/150‑1 (MQ=255)
                                       ccTCGGTCGTGAAGGCTATACCAAAGTACAGAACGCTTCCTACCAGGTTGCTGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACGGGTCGCCAGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGaa                                                                                                       >  1:828749/1‑150 (MQ=255)
                                            gTCGTGAAGGCTATACCAAAGTACAGAACGCTTCCTACCAGGTTGCCGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCcggcgg                                                                                                                       <  1:3025166/129‑1 (MQ=255)
                                              cGTGAAGGCTATACCAAAGTACAGAACGCTTCCTACCAGGTTGCCGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGt                                                                                                <  1:875682/150‑1 (MQ=255)
                                               gTGAAGGCTATACCAAAGTACAGAACGCTTCCTACCAGGTTGCCGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTg                                                                                               >  1:339801/1‑150 (MQ=255)
                                                  taGGCTATACCAAAGTACAGAACGCTTCCTACCAGGTTGCCGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAg                                                                                            <  1:869098/149‑1 (MQ=255)
                                                   aGGCTATACCAAAGTACAGAACGCTTCCTACCAGGTTGCTGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACGGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGa                                                                                           <  1:2722887/150‑1 (MQ=255)
                                                     gCTATACCAAAGTACAGAACGCTTCCTACCAGGTTGCCGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGa                                                                                              >  1:310861/1‑145 (MQ=255)
                                                     gCTATACCAAAGTACAGAACGCTTCCTACCAGGTTGCCGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATc                                                                                         <  1:1417844/150‑1 (MQ=255)
                                                         taCCAAAGTACAGAACGCTTCCTACCAGGTTGCCGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGa                                                                                              >  1:67384/1‑141 (MQ=255)
                                                           ccAAAGTACAGAACGCTTCCTACCAGGTTGCTGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACGGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGAt                                                                                   >  1:3752091/1‑150 (MQ=255)
                                                           ccAAAGTACAGAACGCTTCCTACCAGGTTGCCGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGAt                                                                                   <  1:2464305/150‑1 (MQ=255)
                                                           ccAAAGTACAGAACGCTTCCTACCAGGTTGCCGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGAt                                                                                   <  1:998155/150‑1 (MQ=255)
                                                           ccAAAGTACAGAACGCTTCCTACCAGGTTGCCGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGAt                                                                                   >  1:1324436/1‑150 (MQ=255)
                                                               aGTACAGAACGCTTCCTACCAGGTTGCCGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATacac                                                                               <  1:1820905/150‑1 (MQ=255)
                                                                gTACAGAACGCTTCCTACCAGGTTGCCGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACAcc                                                                              <  1:178099/150‑1 (MQ=255)
                                                                   cAGAACGCTTCCTACCAGATTGCCGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTg                                                                           <  1:2492795/150‑1 (MQ=255)
                                                                     gAACGCTTCCTACCATGTTGCCGCTTACCTGGCGGATGAAATCGCCAAACTTGGGCCGTATGAGTTCAGCTGTACCGTTCGCCCGGACGAAGGGATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTa                                                                         <  1:3504051/150‑1 (MQ=255)
                                                                     gAACGCTTCCTACCAGGTTGCCGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTc                                                                                                               <  1:1703083/112‑1 (MQ=255)
                                                                     gAACGCTTCCTACCAGGTTGCCGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTa                                                                         <  1:1763147/150‑1 (MQ=255)
                                                                     gAACGCTTCCTACCAGGTTGCCGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTa                                                                         <  1:2288457/150‑1 (MQ=255)
                                                                      aaCGCTTCCTACCAGGTTGCCGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTac                                                                        >  1:2542367/1‑149 (MQ=255)
                                                                       aCGCTTCCTACCAGGTTGCTGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACGGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTATg                                                                       <  1:3458471/150‑1 (MQ=255)
                                                                       aCGCTTCCTACCAGGTTGCTGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACGGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTATg                                                                       >  1:1194316/1‑150 (MQ=255)
                                                                       aCGCTTCCTACCAGGTTGCTGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACGGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTATg                                                                       <  1:3211654/150‑1 (MQ=255)
                                                                       aCGCTTCCTACCAGGTTGCTGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACGGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTATg                                                                       >  1:2940819/1‑150 (MQ=255)
                                                                        cGCTTCCTACCAGGTTGCCGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTacga                                                                      >  1:3601309/1‑147 (MQ=255)
                                                                         gCTTCCTACCAGGTTGCCGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTACGAc                                                                     <  1:2932707/150‑1 (MQ=255)
                                                                          cTTCCTACCAGGTTGCCGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCTTGTACGAcc                                                                    >  1:1740780/1‑150 (MQ=255)
                                                                                aCCAGGTTGCCGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTACGACCTCTCTg                                                              <  1:763928/150‑1 (MQ=255)
                                                                                    ggTTGCTGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACGGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTATGACCTCTCTGAAcg                                                          <  1:2018318/150‑1 (MQ=255)
                                                                                    ggTTGCCGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAATGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTACGACCTCTCTGAAcg                                                          <  1:626243/150‑1 (MQ=255)
                                                                                      ttGCCGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTACGACCTCTCTGAAcgtc                                                        >  1:1695329/1‑150 (MQ=255)
                                                                                        gccgctTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTACGACCTCTCTGAAcgtctg                                                      <  1:1561465/147‑1 (MQ=255)
                                                                                         ctgctTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACGGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAGGATGGTGAAGATCCGGGATACACCCTGTATGACCTCTCTGAAcgtctgc                                                     >  1:813138/1‑150 (MQ=255)
                                                                                          tgctTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACGGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGt                                                                                                >  1:2158276/1‑106 (MQ=255)
                                                                                           gctTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACAAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTACGACCTCTCTGAAcgtctgcgt                                                   >  1:3648978/1‑150 (MQ=255)
                                                                                              tATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACGGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTATGACCTCTCTGAAcgtctgcgtctg                                                <  1:1253431/150‑1 (MQ=255)
                                                                                                tCTGGCGGATGAAATCGCCAAACTGGGACCGTATGAGTTCATCTGTACGGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGAGGGTGAAGATCCGGGATACACCCTGTATGACCTCTCTGAACGTCTGCGTCTGCa                                              >  1:3135704/1‑150 (MQ=255)
                                                                                                  tGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTACGACCTCTCTGAACgtctgcgtctgcgcg                                            >  1:2883423/1‑147 (MQ=255)
                                                                                                  tGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTACGACCTCTCTGAACgtctgcgtctgcgcg                                            >  1:2396859/1‑147 (MQ=255)
                                                                                                   ggCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAGGATGGTGAAGATCCGGGATACACCCTGTACGACCTCTCTGAACGTCTGCGTCTGCGCgg                                           >  1:2833487/1‑150 (MQ=255)
                                                                                                    gCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTACGACCTCTCTGAACGTCTGCGTCTGCGCGGc                                          <  1:1200684/150‑1 (MQ=255)
                                                                                                         tGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTACGACCTCTCTGAACGTCTGCGTCTGCGCGGCTGGCa                                     >  1:1277682/1‑150 (MQ=255)
                                                                                                          gAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTACGACCTCTCTGAACGTCTGCGTCTGCGCGGCTGGCAg                                    <  1:1851202/150‑1 (MQ=255)
                                                                                                          gAAATCGCCAAACTGGGGACGTATGAGTTCATCTGTACCGTTCGCCCGGACGAAGGCAGCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATGCACCCTGTACGACCTCTCTGAACGTCTGCGTCTGCGCGGCTGGCAg                                    <  1:3255997/150‑1 (MQ=255)
                                                                                                           aaaTCGCCAAACTGGGGCCGTATGAGTTCGTCTGTACGGGTCGCCCGGACGAAGGTATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTATGACCTCTCTGAACGTCTGCNTCTGCACGGCTGGCAgg                                   <  1:3569706/150‑1 (MQ=255)
                                                                                                           aaaTCGCCAAACTGGGGCCGTATGAGTTCATCTGTACTGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTACGACCTCTCTGAACGTCTGCGTCTGCGCGGCTGGCAgg                                   >  1:1940429/1‑150 (MQ=255)
                                                                                                           aaaTCGCCAAACTGGGGCCGTATGAGTTCATCTGTACGGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTATGACCTCTCTGAACGTCTGCGTCTGCACGGCTGGCAgg                                   >  1:2353061/1‑150 (MQ=255)
                                                                                                           aaaTCGCCAAACTGGGGCCGTATGAGTTCATCTGTACGGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTATGACCTCTCTGAACGTCTGCGTCTGCACGGCTGGCAgg                                   >  1:2506482/1‑150 (MQ=255)
                                                                                                           aaaTCGCCAAACTGGGGCCGTATGAGTTCATCTGTACGGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTATGACCTCTCTGAACGTCTGCGTCTGCACGGCTGGCAgg                                   >  1:2563384/1‑150 (MQ=255)
                                                                                                               cGCCAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTACGACctctc                                                                >  1:1422955/1‑117 (MQ=255)
                                                                                                               cGCCAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTACGACCTCTCTGAACGTCTGCGTCTGCGCGGCTGGCAGGTTcc                               >  1:2125789/1‑150 (MQ=255)
                                                                                                               cGCCAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTACGACCTCTCTGAACGTCTGCGTCTGCGCGGCTGGCAGGTTcc                               >  1:3020578/1‑150 (MQ=255)
                                                                                                                gCCAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTACGACCTCTCTGAACGTCTGCGTCTGCGCGGCTGGCAGGtttcg                              >  1:1076923/1‑147 (MQ=255)
                                                                                                                gCCAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTACGACCTCTCTGAACGTCTGCGTCTGCGCGGCTGGCAGGTTCCg                              >  1:1494693/1‑150 (MQ=255)
                                                                                                                 ccAAACTGGGGCCGTATGAGTTCATCTGTACGGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTATGACCTCTCTGAACGTCTGCGTCTGCACGGCTGGCAGGTTCCgg                             >  1:481957/1‑150 (MQ=255)
                                                                                                                  cAAACTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTACGACCTCTCTGAACGTCTGCGTCTGCGCGGCTGGCAGGTTCCGGc                            >  1:2638981/1‑150 (MQ=255)
                                                                                                                   aaaCTGGGGCCGTATGAGTTCATCTGTACGGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTATGACCTCTCTGAACGTCTGCGTCTGCACGGCTGGCAGGTTCCGGcc                           <  1:2511688/150‑1 (MQ=255)
                                                                                                                     aCTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTACGACCTCTCTGAACGTCTGCGTCTGCGCGGCTGGCAGGTTCCGGcttt                         <  1:1363039/150‑4 (MQ=255)
                                                                                                                      cTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTACGACCTCTCTGAACGTCTGCGTCTGCGCGGCTGGCAGGTTCCGGCTTTc                        <  1:2811886/150‑1 (MQ=255)
                                                                                                                      cTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTACGACCTCTCTGAACGTCTGCGTCTGCGCGGCTGGCAGGTTCCGGCTTTc                        <  1:153296/150‑1 (MQ=255)
                                                                                                                      cTGGGGCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGACATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTACGACCTCTCTGAACGTCTGCGTCTGCGCGGCTGGCAGGTTCCGGCTTTc                        <  1:3535369/150‑1 (MQ=255)
                                                                                                                       ttgggCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTACGACCTCTCTGAACGTCTGCGTCTGCGCGGCTGGCAGGTTCCGGCTTTCa                       <  1:1152498/148‑1 (MQ=255)
                                                                                                                        ggggCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTACGACCTCTCTGAACGTCTGCGTCTGCGCGGCTGGCAGGTTCCGGCTTTCAc                      <  1:2215040/150‑1 (MQ=255)
                                                                                                                         gggCCGTATGAGTTCATCTGTACGGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTATGACCTCTCTGAACGTCTGCGTCTGCACGGCTGGCAGGTTCCGGCCTTTAct                     <  1:2914601/150‑1 (MQ=255)
                                                                                                                         gggCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTACGACCTCTCTGAACGTCTGCGTCTGCGCGGCTGGCAGGTTCCGGCTTTCAct                     >  1:1307321/1‑150 (MQ=255)
                                                                                                                          ggCCGTATGAGTTCATCTGTACGGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTATGACCTCTCTGAACGTCTGCGTCTGCACGGCTGGCAGGTTCCGGCCTTCActc                    >  1:1811939/1‑150 (MQ=255)
                                                                                                                          ggCCGTATGAGTTCATCTGTACGGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTATGACCTCTCTGAACGTCTGCGTCTGCACGGCTGGCAGGTTCCGGCCTTCActc                    >  1:2271496/1‑150 (MQ=255)
                                                                                                                           gCCGTATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTACGACCTCTCTGAACGTCTGCGTCTGCGCGGCTGGCAGGTTCCGGCTTTCActct                   >  1:2853862/1‑150 (MQ=255)
                                                                                                                              gTATGAGTTCATCTGTACGGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTATGACCTCTCTGAACGTCTGCGTCTGCACGGCTGGCAGGTTCCGGCCT‑CACTCTcggc               >  1:60901/1‑150 (MQ=255)
                                                                                                                               tATGAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCCGTACGACCTCTCTGAACGTCTGCGTCTGCGCGGCTGGCAGGTTCCGGCTTTCACTCTcggc               >  1:97624/1‑150 (MQ=255)
                                                                                                                                  gAGTTCATCTGTACGGGTCGCCCGGACGAAGACATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTATGACCTCTCTGAACGTCTGCGTCTGCACGGCTGGCAGGTTCCGGCCTTCACTCTCGGCGGt            >  1:272062/1‑150 (MQ=255)
                                                                                                                                  gAGTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTACGACCTCTCTGAACGTCTGCGTCTGCGCGGCTGGCAGGTTCCGGCTTTCACTCTCGGCGGt            <  1:847629/150‑1 (MQ=255)
                                                                                                                                   aGTTCATCTGTACGGGTCACCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTATGACCTCTCTGAACGTCTGCGTCTGCACGGCTGGCAGGTTCCGGCCTTCACTCTCGGCGGTg           >  1:2373218/1‑150 (MQ=255)
                                                                                                                                    gTTCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTACGACCTCTCTGAACGTCTGCGTCTGCGCGGCTGGCAGGTTCCGGCTTTCACTCTCGGAGGTGa          >  1:3204708/1‑150 (MQ=255)
                                                                                                                                      tCATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACGCCCTGTACGACCTCTCTGAACGTCTGCGTCTGCGCGGCTGGCAGGTTCCGGCTTTCACTCTCGGCGGTGAAg        <  1:3363280/150‑1 (MQ=255)
                                                                                                                                       cATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTACGACCTCTCTGAACGTCTGCGTCTGCGCGGCTGGCAGGTTCCGGCTTTCACTCTCGGCGGTgg          >  1:2269688/1‑146 (MQ=255)
                                                                                                                                       cATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTACGACCTCTCTGAACGTCTGCGTCTGCGCGGCTGGCAGGTTCCGGCTTTCACTCTCGGCGGTGAAGc       >  1:3101178/1‑150 (MQ=255)
                                                                                                                                       cATCTGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTACGACCTCTCTGAACGTCTGCGTCTGCGCGGCTGGCAGGTTCCGGCTTTCACTCTCGGCGGTGAAGc       >  1:2493610/1‑150 (MQ=255)
                                                                                                                                          cTGTACGGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTATGACCTCTCTGAACGTCTGCGTCTGCACGGCTGGCAGGTTCCGGCCTTCACTCTCGGCGGTGAAGCCAc    >  1:1641284/1‑150 (MQ=255)
                                                                                                                                           tGTACCGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGACCCGGGATACACCCTGTACGACCTCTCTGAACGTNTGCGTCTGCGCGGCTGGCAGGTTCCGGCTTTCACTCTCGGCGGTGAAGCCAcc   <  1:3824094/150‑2 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
‑‑‑‑‑‑‑‑‑‑‑‑‑ATTGCACAGTACTATGAATTCCTGCGCCTCGGTCGTGAAGGCTATACCAAAGTACAGAACGCTTCCTACCAGGTTGCTGCTTATCTGGCGGATGAAATCGCCAAACTGGGGCCGTATGAGTTCATCTGTACGGGTCGCCCGGACGAAGGCATCCCGGCGGTTTGCTTCAAACTGAAAGATGGTGAAGATCCGGGATACACCCTGTATGACCTCTCTGAACGTCTGCGTCTGCACGGCTGGCAGGTTCCGGCCTTCACTCTCGGCGGTGAAGCCACTG  >  Xen14_28/1‑277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑