Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	Xen14_29	61,551	+T	coding (1592/2127 nt)	ycjT →	Kojibiose phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Xen14_29	61,551	1	.	T	85.1%	113.3 / 16.9	47	coding (1592/2127 nt)	ycjT	Kojibiose phosphorylase
Reads supporting (aligned to +/- strand):  ref base . (3/4);  new base T (20/20);  total (23/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTATATGGCGTATTACAACGTCCAGCAGGCGCTGAATATCGCTCGTCAGTTTGGCTGTAGCGACGACGCGTTTATTCATCGTGCAGAAATGTACCTCAAAGAACTGCGGCTGCCAGAAATTCAGCCCGACGGCGTTTT‑GCCGCAGGATGATTCGTTTATGGCTAAGCCGGTGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATTCTGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCA  >  Xen14_29/61413‑61700                                                                                                                                            |                                                                                                                                                      gCTATATGGCGTATTACAACGTCCAGCAGGCGCTGAATATCGCTCGTCAGTTTGGCTGTAGCGACGACGCGTTTATTCATCGTGCAGAAATGTACCTCAAAGAACTGCGGCTGCCAGAAATTCAGCCCGACGGCGTTTTTGCCGCAGgat                                                                                                                                             <  1:536851/150‑1 (MQ=255)         gCGTATTACAACGTCCAGCAGGCGCTGAATATCGCTCGTCAGTTTGGCTGTAGCGACGACGCGTTTATTCATCGTGCAGAAATGTACCTCAAAGAACTGCGGCTGCCAGAAATTCAGCCCGACGGCGTTTTTGCCGCAGGATGATTCGtt                                                                                                                                     <  1:3556319/150‑1 (MQ=255)             aTTACAACGTCCAGCAGGCGCTGAATATCGCTCGTCAGTTTGGCTGTAGCGACGACGCGTTTATTCATCGTGCAGAAATGTACCTCAAAGAACTGCGGCTGCCAGAAATTCAGCCCGACGGCGTTTTTGCCGCAGGATGATTCGTTTATg                                                                                                                                 >  1:997471/1‑150 (MQ=255)             aTTACAACGTCCAGCAGGCGCTGAATATCGCTCGTCAGTTTGGCTGTAGCGACGACGCGTTTATTCATCGTGCAGAAATGTACCTCAAAGAACTGCGGCTGCCAGAAATTCAGCCCGACGGCGTTTTTGCCGCAGGATGATTCGTTTATg                                                                                                                                 >  1:997470/1‑150 (MQ=255)                        cagcagGCGCTGAATATCGCTCGTCAGTTTGGCTGTAGCGACGACGCGTTTATTCATCGTGCAGAAATGTACCTCAAAGAACTGCGGCTGCCAGAAATTCAGCCCGACGGCGTTTT‑GCCGCAGGATGATTCGTTTATGGCTAAGCCGGTg                                                                                                                     >  1:1806609/1‑150 (MQ=255)                         agcagGCGCTGAATATCGCTCGTCAGTTTGGCTGTAGCGACGACGCGTTTATTCATCGTGCAGAAATGTACCTCAAAGAACTGCGGCTGCCAGAAATTCAGCCCGACGGCGTTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGTg                                                                                                                     <  1:3294863/150‑1 (MQ=255)                           cagGCGCTGAATATCGCTCGTCAGTTTGGCTGTAGCGACGACGCGTTTATTCATCGTGCAGAAATGTACCTCAAAGAACTGCGGCTGCCAGAAATTCAGCCCGACGGCGTTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGTGAt                                                                                                                   <  1:2739275/150‑1 (MQ=255)                           cagGCGCTGAATATCGCTCGTCAGTTTGGCTGTAGCGACGACGCGTTTATTCATCGTGCAGAAATGTACCTCAAAGAACTGCGGCTGCCAGAAATTCAGCCCGACGGCGTTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGTGAt                                                                                                                   <  1:2108790/150‑1 (MQ=255)                              gcgcTGAATATCGCTCGTCAGTTTGGCTGTAGCGACGACGCGTTTATTCATCGTGCAGAAATGTACCTCAAAGAACTGCGGCTGCCAGAAATTCAGCCCGACGGCGTTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGTGATTaa                                                                                                                >  1:2095038/1‑150 (MQ=255)                                    aaTATCGCTCGTCAGTTTGGCTGTAGCGACGACGCGTTTATTCATCGTGCAGAAATGTACCTCAAAGAACTGCGGCTGCCAGAAATTCAGCCCGACGGCGTTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGTGATTAATCTGGc                                                                                                          <  1:2464600/150‑1 (MQ=255)                                    aaTATCGCTCGTCAGTTTGGCTGTAGCGACGACGCGTTTATTCATCGTGCAGAAATGTACCTCAAAGAACTGCGGCTGCCAGAAATTCAGCCCGACGGCGTTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGTGATTAAACTGGc                                                                                                          <  1:1943364/150‑1 (MQ=255)                                     atatCGCTCGTCAGTTTGGCTGTAGCGACGACGCGTTTATTCATCGTGCAGAAATGTACCTCAAAGAACTGCGGCTGCCAGAAATTCAGCCCGACGGCGTTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGTGATTAATCTGGCg                                                                                                         >  1:242335/1‑150 (MQ=255)                                        tCGCTCGTCAGTTTGGCTGTAGCGACGACGCGTTTATTCATCGTGCAGAAATGTACCTCAAAGAACTGCGGCTGCCAGAAATTCAGCCCGACGGCGTTTT‑GCCGCAGNATGATTCGTTTATGGCTAAGCCGGTGATTAATCTGGCGAAAt                                                                                                     <  1:2377191/150‑1 (MQ=255)                                              gTCAGTTTGGCTGTAGCGACGACGCGTTTATTCGTCGTGCAGAAATGTACCTCAAAGAACTGCGGCTGCCAGAAATTCAGCCCGACGGTGTTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGTGATTAATCTGGCGAAATACaaa                                                                                                <  1:1362/150‑1 (MQ=255)                                              gTCAGTTTGGCTGTAGCGACGACGCGTTTATTCGTCGTGCAGAAATGTACCTCAAAGAACTGCGGCTGCCAGAAATTCAGCCCGACGGTGTTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGTGATTAATCTGGCGAAATACaaa                                                                                                <  1:1360/150‑1 (MQ=255)                                              gTCAGTTTGGCTGTAGCGACGACGCGTTTATTCATCGTGCAGAAATGTACCTCAAAGAACTGCGGCTGCCAGAAATTCAGCCCGACGGTGTTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGTGATTAATCTGGCGAAATACaaa                                                                                                >  1:1469829/1‑150 (MQ=255)                                                  gTTTGGCTGTAGCGACGACGCGTTTATTCATCGTGCAGAAATGTACCTCAAAGAACTGCGGCTGCCAGAAATTCAGCCCGACGGCGTTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGTGATTAATCTGGCGAAATACAAAgcgg                                                                                            >  1:1979810/1‑150 (MQ=255)                                                                 cgacgCGTTTATTCATCATGCAGAAATGTACCTCAAAGAACTGCGGCTGCCAGAAATTCAGCCCGACGGCGTTTT‑GCCGCAGGATGATTCGTTTATGGCTAAGCCGGTGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAAc                                                                            <  1:2287348/150‑1 (MQ=255)                                                                          tATTCATCGTGCAGAAATGTACCTCAAAGAACTGCGGCTGCCAGAAATTCAGCCCGACGGCGTTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGTGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATTctgc                                                                    >  1:3453632/1‑150 (MQ=255)                                                                           aTTCATCGTGCAGAAATGTACCTCAAAGAACTGNGGCTGCCAGAAATTCAGCCCGACGGCGTTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGTGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATTctgct                                                                   <  1:1775879/150‑1 (MQ=255)                                                                           aTTCATCGTGCAGAAATGTACCTCAAAGAACTGCGGCTGCCAGAAATTCAGCCCGACGGCGTTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGTGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATTctgct                                                                   <  1:659812/150‑1 (MQ=255)                                                                           aTTCATCGTGCAGAAATGTACCTCAAAGAACTGCGGCTGCCAGAAATTCAGCCCGACGGCGTTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGTGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATTctgct                                                                   <  1:659814/150‑1 (MQ=255)                                                                            ttCATCGTGCAGAAATGTACCTCAAAGAACTGCGGCTGCCAGAAATTCAGCCCGACGGCGTTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGTGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATTctgctg                                                                  <  1:3633164/150‑1 (MQ=255)                                                                                  gTGCAGAAATGTACCTCAAAGAACTGCGGCTGCCAGAAATTCAGCCCGACGGCGTTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGTGATTAATCTGGCGAAATACAAAGCGGCTGCGGGGAAGCAAACCATTCTGCTGGattat                                                            >  1:3506184/1‑150 (MQ=255)                                                                                  gTGCAGAAATGTACCTCAAAGAACTGCGGCTGCCAGAAATTCAGCCCGACGGCGTTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGTGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATTCTGCTGGattat                                                            >  1:1922262/1‑150 (MQ=255)                                                                                   tGCAGAAATGTACCTCAAAGAACTGCGGCTGCCAGAAATTCAGCCCGACGGCGTTTT‑GCCGCAGGATGATTCGTTTATGGCTAAGCCGGTGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATTCTGCTGGATTATTc                                                          <  1:1828600/150‑1 (MQ=255)                                                                                   tGCAGAAATGTACCCCAAAGAACTGCGGCTGCCAGAAATTCAGCCCGACGGCGTTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGTGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATTCTGCTGGattatt                                                           <  1:24047/150‑1 (MQ=255)                                                                                     cAGAAATGTACCTCAAAGAACTGCGGCTGCCAGAAATTCAGCCCGACGGCGTTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGTGATTAATCTGGCGAAATACAAAgc                                                                                              <  1:897305/113‑1 (MQ=255)                                                                                         aaTGTACCTCAGAGAACTGCGGCTGCCAGAAATTCAGCCCGACGGCGTTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGTGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATTCTGCTGGATTATTCAcgcg                                                     >  1:2376020/1‑150 (MQ=255)                                                                                         aaTGTACCTCAGAGAACTGCGGCTGCCAGAAATTCAGCCCGACGGCGTTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGTGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATTCTGCTGGATTATTCAcgcg                                                     >  1:2376019/1‑150 (MQ=255)                                                                                           tGTACCTCAAAGAACTGCGGCTGCCAGAAATTCAGCCCGACGGCGTTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGTGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATTCTGCTGGATTATTCACGCGCa                                                   >  1:2426322/1‑150 (MQ=255)                                                                                              aCCTCAAAGAACTGCGGCTGCCAGAAATTCAGCCCGACGGCTTTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGTGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATTCTGCTGGATTATTCACGCGCAGaa                                                <  1:3773935/150‑1 (MQ=255)                                                                                                cTCAAAGAACTGCGGCTGCCAGAAATTCAGCCCGACGGCGTTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGTGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATTCTGCTGGATTATTCACGCGCAGAAGt                                              >  1:1574933/1‑150 (MQ=255)                                                                                                   aaaGAACTGCGGCTGCCAGAAATTCAGCCCGACGGCGTTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGTGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATTCTGCTGGATTATTCACGCGCAGAAGTGaa                                           <  1:415048/150‑1 (MQ=255)                                                                                                    aaGAACTGCGGCTGCCAGAAATTCAGCCCGACGGCGTTTT‑GCCGCAGGATGATTCGTTTATGGCTAAGCCGGTGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATTCTGCTGGATTATTCACGCGCAGAAGTGAACg                                         <  1:1048024/150‑1 (MQ=255)                                                                                                         cTGCGGCTGCCAGAAATTCAGCCCGACGGCGTTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGTGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGGAGCAAACCATTCTGCTGGATTATTCACGCGCAGAAGTGAACGAGAt                                     <  1:1152859/150‑1 (MQ=255)                                                                                                                 gCCAGAAATTCAGCCCGACGGCGTTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGTGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATTCTGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATcc                             >  1:3395271/1‑150 (MQ=255)                                                                                                                  ccAGAAATTCAGCCCGACGGCGTTTT‑GCCGCAGGATGATTCGTTTATGGCTAAGCCGGTGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATTCTGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTc                           >  1:1603186/1‑150 (MQ=255)                                                                                                                   cAGAAATTCAGCCCGACGGCGTTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGTGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATTCTGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGa                                <  1:2448986/145‑1 (MQ=255)                                                                                                                   cAGAAATTCAGCCCGACGGCGTTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGTGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATTCTGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTc                           >  1:1759599/1‑150 (MQ=255)                                                                                                                   cAGAAATTCAGCCCGACGGCGTTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGTGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATTCTGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTc                           >  1:1759595/1‑150 (MQ=255)                                                                                                                   cAGAAATTCAGCCCGACGGCGTTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGTGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATTCTGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTc                           >  1:1759600/1‑150 (MQ=255)                                                                                                                   cAGAAATTCAGCCCGACGGCGTTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGTGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATTCTGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTc                           >  1:232313/1‑150 (MQ=255)                                                                                                                   cAGAAATTCAGCCCGACGGCGTTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGTGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATTCTGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTc                           >  1:232315/1‑150 (MQ=255)                                                                                                                    aGAAATTCAGCCCGACGGCGTTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGTGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATTCTGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCa                          <  1:1611583/150‑1 (MQ=255)                                                                                                                            aGCCCGACGGCGTTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGTGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATTctgct                                                                   >  1:596369/1‑101 (MQ=255)                                                                                                                            aGCCCGACGGCGTTTT‑GCCGCAGGATGATTCGTTTATGGCTAAGCCGGTGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATTCTGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTg                 >  1:1166535/1‑150 (MQ=255)                                                                                                                                        tttt‑GCCGCAGGATGATTCGTTTATGGCTAAGCCGGTGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATTCTGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGc     <  1:3418558/150‑1 (MQ=255)                                                                                                                                        tttt‑GCCGCAGGATGATTCGTTTATGGCTAAGCCGGTGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATTCTGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGc     <  1:3283125/150‑1 (MQ=255)                                                                                                                                           t‑GCCGCAGGATGATTCGTTTATGGCTAAGCCGGTGATTAATCTGGAGAAATACAAAGCGGCGGCGGGGAAGCAAACCATTCTGCTGGATTATTCACGCGCAGAAGTGGACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCa  >  1:1477812/1‑150 (MQ=255)                                                                                                                                            |                                                                                                                                                      GCTATATGGCGTATTACAACGTCCAGCAGGCGCTGAATATCGCTCGTCAGTTTGGCTGTAGCGACGACGCGTTTATTCATCGTGCAGAAATGTACCTCAAAGAACTGCGGCTGCCAGAAATTCAGCCCGACGGCGTTTT‑GCCGCAGGATGATTCGTTTATGGCTAAGCCGGTGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATTCTGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCA  >  Xen14_29/61413‑61700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑