Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	Xen14_106	3,879	(T)10→11	coding (315/357 nt)	Xen14_04646 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Xen14_106	3,879	1	.	T	95.7%	187.9 / 2.3	70	coding (315/357 nt)	Xen14_04646	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (1/2);  new base T (38/29);  total (39/31)
Fisher's exact test for biased strand distribution p-value = 5.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.02e-01

AACTATATCATTATAATCATTAAAAAAGGATAACAAATACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAATTTCCATGTCCCAATGAGTGTTTTTTGCTAAAAAAAACAAGGAGTTG  >  Xen14_106/3730‑4026                                                                                                                                                        |                                                                                                                                                    aaCTATATCATTATAATCATTAAAAAAGGATAACAAATACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑tttttttttt                                                                                                                                                      >  1:3165338/1‑150 (MQ=255)     atatCATTATAATCATTAAAAAAGGATAACAAATACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑tttttttttttaca                                                                                                                                                  >  1:1646556/1‑146 (MQ=255)             ataATCATTAAAAAAGGATAACAAATACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAaga                                                                                                                                          >  1:944694/1‑150 (MQ=255)              taATCATTAAAAAAGGATAACAAATACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAagag                                                                                                                                         <  1:2537108/150‑1 (MQ=255)                 tCATTAAAAAAGGATAACAAATACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGtca                                                                                                                                      >  1:1960723/1‑150 (MQ=255)                    ttAAAAAAGGATAACAAATACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGtcatca                                                                                                                                   >  1:2210334/1‑150 (MQ=255)                     tAAAAAAGGATAACAAATACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAg                                                                                                                                  <  1:3474697/150‑1 (MQ=255)                      aaaaaaGGATAACAAATACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAga                                                                                                                                 >  1:69031/1‑150 (MQ=255)                        aaaaGGAGAACAAATACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAgatg                                                                                                                               <  1:3202413/150‑1 (MQ=255)                             gagaaCAAATACAATATTTTAAATATTTTTTTGTTGGTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTTAATTGCATTTCATTGAGGTTCGCAGTAAATAGTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATAtt                                                                                                                          <  1:183202/147‑1 (MQ=255)                             gATAACAAATACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATAtt                                                                                                                          <  1:183205/150‑1 (MQ=255)                             gATAACAAATACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATAtt                                                                                                                          <  1:183204/150‑1 (MQ=255)                             gATAACAAATACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATAtt                                                                                                                          <  1:183201/150‑1 (MQ=255)                                 aCAAATACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGACATTGAAt                                                                                                                      >  1:43777/1‑150 (MQ=255)                                   aaaTACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATgc                                                                                                                    >  1:1632570/1‑150 (MQ=255)                                      tACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATgcagc                                                                                                                 <  1:2600971/150‑1 (MQ=255)                                      tACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATgcagc                                                                                                                 >  1:3533768/1‑150 (MQ=255)                                         aaTATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAAc                                                                                                              >  1:398678/1‑150 (MQ=255)                                                tAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACttaatta                                                                                                       <  1:248299/150‑1 (MQ=255)                                                tAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACttaatta                                                                                                       <  1:248294/150‑1 (MQ=255)                                                    tatTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTCATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGc                                                                                                   >  1:1434921/1‑150 (MQ=255)                                                    tatTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTCAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGc                                                                                                   >  1:1434925/1‑150 (MQ=255)                                                    tatTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGc                                                                                                   >  1:2656150/1‑150 (MQ=255)                                                      tttttttGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGcat                                                                                                 >  1:3353116/1‑150 (MQ=255)                                                                 ttACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATtata                                                                                      <  1:2639064/150‑1 (MQ=255)                                                                      tGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGTATCATCAATTATAAtttt                                                                                 >  1:3808370/1‑150 (MQ=255)                                                                      tGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAAtttt                                                                                 >  1:1815003/1‑150 (MQ=255)                                                                      tGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAAtttt                                                                                 >  1:3808371/1‑150 (MQ=255)                                                                          tATTTTATGTCTGTAATTATATCGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTg                                                                             <  1:2544040/150‑1 (MQ=255)                                                                           aTTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGt                                                                            <  1:2288454/150‑1 (MQ=255)                                                                             tttATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTAc                                                                          >  1:1632918/1‑150 (MQ=255)                                                                                   tCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTa                                                                    >  1:221316/1‑150 (MQ=255)                                                                                   tCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTa                                                                    >  1:221312/1‑150 (MQ=255)                                                                                   tCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTa                                                                    >  1:221311/1‑150 (MQ=255)                                                                                   tCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTa                                                                    >  1:221308/1‑150 (MQ=255)                                                                                    cTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTAc                                                                   >  1:3127691/1‑150 (MQ=255)                                                                                    cTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTAc                                                                   >  1:3127692/1‑150 (MQ=255)                                                                                      gTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACta                                                                 >  1:1386702/1‑150 (MQ=255)                                                                                         aTTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACtatta                                                              >  1:3383398/1‑150 (MQ=255)                                                                                          ttATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAGCTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACtattat                                                             >  1:3012334/1‑150 (MQ=255)                                                                                          ttATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAGCTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACtattat                                                             >  1:3113544/1‑150 (MQ=255)                                                                                           tataTTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATc                                                            <  1:1184373/150‑1 (MQ=255)                                                                                           tataTTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATc                                                            >  1:1576688/1‑150 (MQ=255)                                                                                           tataTTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATc                                                            <  1:1184370/150‑1 (MQ=255)                                                                                              atTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGAt                                                         >  1:1543470/1‑150 (MQ=255)                                                                                               ttGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACtatta                                                              <  1:2941007/144‑1 (MQ=255)                                                                                                 ggAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTcc                                                      <  1:3592964/150‑1 (MQ=255)                                                                                                 ggAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTcc                                                      <  1:744353/150‑1 (MQ=255)                                                                                                 ggAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTcc                                                      <  1:1534605/150‑1 (MQ=255)                                                                                                  gAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCg                                                     >  1:1390378/1‑150 (MQ=255)                                                                                                  gAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCg                                                     >  1:1390377/1‑150 (MQ=255)                                                                                                  gAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCg                                                     >  1:1390372/1‑150 (MQ=255)                                                                                                  gAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCg                                                     <  1:3398423/150‑1 (MQ=255)                                                                                                      cTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTACGACaa                                                 <  1:896654/150‑1 (MQ=255)                                                                                                       tataTTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAAtt                                               >  1:1087303/1‑150 (MQ=255)                                                                                                        atatTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACCTAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATACATCATCAATGATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAAtt                                               >  1:3516889/1‑150 (MQ=255)                                                                                                          atTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAATTTc                                             >  1:3075557/1‑150 (MQ=255)                                                                                                          atTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAATTTc                                             >  1:3075555/1‑150 (MQ=255)                                                                                                            tCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAATTTCCa                                           <  1:3308820/150‑1 (MQ=255)                                                                                                            tCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAATTTCCa                                           <  1:3562306/150‑1 (MQ=255)                                                                                                                 tGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAATTTCCATGTcc                                      >  1:54157/1‑150 (MQ=255)                                                                                                                  gCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAATTTCCATGTccc                                     >  1:901530/1‑150 (MQ=255)                                                                                                                         aTTGAGGTTCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCCGATGATATTGAATGCCGCAACTTAATTAATGCATCATCAATTATAGTTTTTATGTACGGATTCCTATTCTCGATTCCGACACTTTCCATGTCCCAATgcgt                              >  1:786717/1‑147 (MQ=255)                                                                                                                                ttCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAATTTCCATGTCCCAATGAGTGtttttt                       <  1:3258499/150‑1 (MQ=255)                                                                                                                                 tCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAATTTCCATGTCCCAATGAGTGTTTTTTg                      <  1:3454971/150‑1 (MQ=255)                                                                                                                                 tCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAATTTCCATGTCCCAATGAGTGTTTTTTg                      <  1:3454970/150‑1 (MQ=255)                                                                                                                                 tCGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAATTTCCATGTCCCAACGAGTGTTTTTTg                      <  1:767316/150‑1 (MQ=255)                                                                                                                                  cGCAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAATTTCCATGTCCCAATGAGTGTTTTTTGc                     <  1:1266964/150‑1 (MQ=255)                                                                                                                                   gCAGTAAATA‑TTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAATTTCCATGTCCCAATGAGTGTTTTTTGCTa                   <  1:2175570/150‑1 (MQ=255)                                                                                                                                    cAGTAAATA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAATTTCCATGTCCCAATGAGTGTTTTTTGCTa                   <  1:376668/150‑1 (MQ=255)                                                                                                                                        aaaTA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAATTTCCATGTCCCAATGAGTGTTTTTTGCt                    >  1:3293284/1‑145 (MQ=255)                                                                                                                                        aaaTA‑TTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAATTTCCATGTCCCAATGAGTGTTTTTTGCTaaaaa               <  1:2524006/150‑1 (MQ=255)                                                                                                                                                 ttttttt‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCCGTACGGATTACTATTATCGATTCCGACAATTTCCATGTCCCAATGAGTGTTTTTTGCTAAAAAAAACAAGGa      >  1:577351/1‑150 (MQ=255)                                                                                                                                                  tttttt‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAATTTCCATGTCCCAATGAGTGTTTTTTGCTAAAAAAAACAAGGAg     >  1:1089003/1‑150 (MQ=255)                                                                                                                                                     ttt‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAATTTCCATGTCCCAATGAGTGTTTTTTGCTAAAAAAAACAAGGAGTTg  <  1:3759957/150‑1 (MQ=255)                                                                                                                                                     ttt‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAATTTCCATGTCCCAATGAGTGTTTTTTGCTAAAAAAAACAAGGAGTTg  >  1:2827714/1‑150 (MQ=255)                                                                                                                                                        |                                                                                                                                                    AACTATATCATTATAATCATTAAAAAAGGATAACAAATACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATA‑TTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAATTTCCATGTCCCAATGAGTGTTTTTTGCTAAAAAAAACAAGGAGTTG  >  Xen14_106/3730‑4026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑