FastQCFastQC Report
Wed 6 Sep 2023
6183_ctrl3_S3_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename6183_ctrl3_S3_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54995055
Sequences flagged as poor quality0
Sequence length200
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGTTCCAGTGTGGCTGATCATCCTCTCAGACCAGCTAGGGATCGTCG9100331.654754231994131No Hit
GTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACT6127891.1142620004653145No Hit
CCCCTCTACAGTACTCTAGCTTGTCAGTTTCAAATGCGATTCCTAGGTTG5695021.0355512872930122No Hit
CTGGCAAACAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACAT4418530.8034413275884531No Hit
CCCCCCTCTACAGTACTCTAGCTTGTCAGTTTCAAATGCGATTCCTAGGT3928070.7142587638106735No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGT3913590.7116257998105466No Hit
CCTAGCGCTACCTTGTTACGACTTCACCCCAGTCATGAACCACAAAGTGG3364090.6117077253582164No Hit
TTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAG2931680.5330806560698957No Hit
CGTGTCTCAGTTCCAGTGTGGCTGATCATCCTCTCAGACCAGCTAGGGAT2875180.5228070051025496No Hit
CAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGC2617950.4760337088489138No Hit
CTCTACAGTACTCTAGCTTGTCAGTTTCAAATGCGATTCCTAGGTTGAGC2515910.4574793133673564No Hit
CACGTGTGTAGCCCTACTCGTAAGGGCCATGATGACTTGACGTCGTCCCC2514780.45727384034800944No Hit
CGGGCAATTAGTACAGGTTAGCTCAACGCCTCACAACGCTTACACACCCT2418110.4396958962946759No Hit
CCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTG2340560.4255946284625045No Hit
ATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGA2261240.41117151351153297No Hit
CCGTGTCTCAGTTCCAGTGTGGCTGATCATCCTCTCAGACCAGCTAGGGA2231900.40583648838972886No Hit
CGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATT2203840.400734211466831No Hit
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC2138160.38879131951045415No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATATCA2121150.3856983141484266No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT2067280.37590288799602073No Hit
CGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCG2049980.3727571506201785No Hit
CCCAGGCGGTCTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGGC1771220.32206895692712734No Hit
CCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGG1677120.30495832761691033No Hit
CGTCAATTCATTTGAGTTTTAATCTTGCGACCGTACTCCCCAGGCGGTCT1673560.30431099668870226No Hit
CCTAGCGCTACCTTGTTCCGACTTCACCCCAGTCATGAACCACAAAGTGG1666280.302987241307423No Hit
CAACGTTCTAGTCTCGAACAACCCTTTAGGACAGTTAAACTGTCAGGGAA1635950.2974722000005273No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGATCATCCTCTCAGACCAGCT1606370.2920935345914283No Hit
GGCTGATCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCT1573090.2860420814198658No Hit
CGATTCCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCATGGCATC1475930.2683750384466385No Hit
GCTCGCTTCGCGCTTAGATGCTTTCAGCGCTTATCAATTCCGAACTTAGC1453920.2643728604326334No Hit
CCTGCATGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCG1426870.259454236385435No Hit
GTACGATTCCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCATGGC1420000.25820503316161786No Hit
GTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGACG1391130.25295547026909965No Hit
GTGTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTA1381800.2512589541005096No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGC1374350.24990428684906305No Hit
TGGCAAACAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATT1371380.2493642382937884No Hit
CTCGAACAACCCTTTAGGACAGTTAAACTGTCAGGGAAGACTCATCTCAG1364630.24813685521361872No Hit
CGCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTG1361810.24762408183790344No Hit
GTCAGTATCTGTCCAGGGGGCCGCCTTCGCCACCGGTATTCCTTCAGATC1360040.24730223471910337No Hit
CCTAGCGCTACCTTGTTTCGACTTCACCCCAGTCATGAACCACAAAGTGG1314050.23893966466621408No Hit
TACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTA1249090.22712769357172202No Hit
GGGTACGATTCCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCATG1243380.2260894184031637No Hit
CCCCAGGCGGTCTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGG1230060.22366738245829554No Hit
CTCGCTTCGCGCTTAGATGCTTTCAGCGCTTATCAATTCCGAACTTAGCT1211840.22035435731448944No Hit
CTAGTCTCGAACAACCCTTTAGGACAGTTAAACTGTCAGGGAAGACTCAT1135820.2065312963138231No Hit
GTTAGCTCAACGCCTCACAACGCTTACACACCCTGCCTATCAACGTTCTA1116730.20306007512857294No Hit
CTTGGTATTCTCTACCCGACCACCTGTGTCGGTTTGGGGTACGATTCCTG1076970.19583033420004764No Hit
CACCTGCATGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTC1069120.1944029331364429No Hit
CCCGGTTTCGGGTCTATATCCAGAGACTGAGCGCCCAGTTAAGACTCGCT1063650.1934082982551795No Hit
ATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAG1057850.19235365797888557No Hit
CTGGAGTTCCCACCATTACGTGCTGGCAAACAAGGATAAGGGTTGCGCTC1057660.19231910941811042No Hit
CTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCTTACCTCACCAA1040760.18924610585442636No Hit
AGGCGGTCTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGGCACA1030890.18745139903942273No Hit
GGTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGAC1017420.18500208791499526No Hit
CTCCCCAGGCGGTCTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAG1011070.1838474386469838No Hit
CCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTG1009790.18361469044807754No Hit
CCCACCTTCGTGCTCCTCCGTTACTCTTTGGGAGGAGACCGCCCCAGTCA997220.18132903040100606No Hit
CCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTT995360.1809908181744704No Hit
GGCAATTAGTACAGGTTAGCTCAACGCCTCACAACGCTTACACACCCTGC981920.17854696208595483No Hit
GTCTCGAACAACCCTTTAGGACAGTTAAACTGTCAGGGAAGACTCATCTC981450.17846149985666893No Hit
CTGGCACGGAGTTAGCCGGTGCTTCTTCTGTAGGTAACGTCAAATGATTA972810.17689044951405178No Hit
GTACAGGTTAGCTCAACGCCTCACAACGCTTACACACCCTGCCTATCAAC967460.1759176347764358No Hit
GTCTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGGCACAACCTC966620.17576489377090357No Hit
CCTGGAGTTCCCACCATTACGTGCTGGCAAACAAGGATAAGGGTTGCGCT963020.17511028946147977No Hit
CTAGCGCTACCTTGTTACGACTTCACCCCAGTCATGAACCACAAAGTGGC958570.17430112580121976No Hit
CTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGACGACAGCC953220.1733283110636038No Hit
ACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAG949240.1726046096326297No Hit
CCCTGCTTTGCTCTTGCGAGGTTATGCGGTATTAGCCATCGTTTCCAATG941450.1711881186408487No Hit
GGCTCGCTTCGCGCTTAGATGCTTTCAGCGCTTATCAATTCCGAACTTAG932520.16956433628441686No Hit
CTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGGCACAACCTCCA923210.16787145680643467No Hit
CTTCAATCTTCCAACGCCCACGGCAGATAGGGACCGAACTGTCTCACGAC903630.1643111367012907No Hit
GGCAAACAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTT903470.1642820431764274No Hit
CCCTGGAGTTCCCACCATTACGTGCTGGCAAACAAGGATAAGGGTTGCGC896740.16305829678686565No Hit
CCTGCTTTCGCACCTGCTCGAACCGTCATTCTCGCAGTTAAGCGGGCTTA892620.16230913852163617No Hit
CAGGTTAGCTCAACGCCTCACAACGCTTACACACCCTGCCTATCAACGTT873910.15890701445793629No Hit
CTACAGTACTCTAGCTTGTCAGTTTCAAATGCGATTCCTAGGTTGAGCCC868080.15784691914573049No Hit
CAGGCGGTCTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGGCAC867670.1577723669882683No Hit
GGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTG862290.1567940972147405No Hit
CTCCAATCCGGACTACGACGTACTTTGTGAGATTCGCTCCACCTCGCGGT849470.1544629785350701No Hit
CCTCTGTATACGCCATTGTAGCACGTGTGTAGCCCTACTCGTAAGGGCCA845020.1536538148748101No Hit
GGTGTGTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGA843110.1533065109217547No Hit
CTCCGTTACTCTTTGGGAGGAGACCGCCCCAGTCAAACTACCCACCAGGC834600.15175909906808893No Hit
CACGGAGTTAGCCGGTGCTTCTTCTGTAGGTAACGTCAAATGATTAAGGT834430.1517281871979217No Hit
TGATCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCTTAC825830.15016441023652036No Hit
CTTTAGGACAGTTAAACTGTCAGGGAAGACTCATCTCAGGGCTCGCTTCG822230.14950980592709653No Hit
CGCGTTAGCTCCGAAAGCCACACCTCTAGGGCACAACCTCCAAGTAGACA816540.1484751674491461No Hit
CCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCA813480.14791875378613586No Hit
CCCTAGCGCTACCTTGTTACGACTTCACCCCAGTCATGAACCACAAAGTG799460.1453694336699909No Hit
CCTTGGTATTCTCTACCCGACCACCTGTGTCGGTTTGGGGTACGATTCCT797740.1450566782777106No Hit
CCAGGCGGTCTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGGCA796710.14486938871140326No Hit
GGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTCC795370.14462573044067326No Hit
CAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAT793820.14434388691856023No Hit
GTTTTAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTAACGCGTTAGC783490.14246553621957464No Hit
CAGGGCTCGCTTCGCGCTTAGATGCTTTCAGCGCTTATCAATTCCGAACT770050.14002168013105906No Hit
GTCGACGTATAAGCCGGGTTCTGTTCCGCTTGCGCGGTAGTAGCCATTCC766240.1393288905702522No Hit
CTGGGTTGTTTCCCTCTCCACGACGGACGTTAGCACCCGCCGTGTGTCTC761730.13850881683816846No Hit
CGACCGTACTCCCCAGGCGGTCTACTTAACGCGTTAGCTCCGAAAGCCAC760260.13824152007848706No Hit
CTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACC744480.13537217118884598No Hit
CCCGTCAATTCATTTGAGTTTTAATCTTGCGACCGTACTCCCCAGGCGGT742350.13498486363910353No Hit
GTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCA739540.13447390860869218No Hit
ATGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTA737190.13404659746226275No Hit
GCTGATCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCTT726520.1321064230229427No Hit
CTCACGGGCAATTAGTACAGGTTAGCTCAACGCCTCACAACGCTTACACA723920.13163365324391438No Hit
GCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGC723060.13147727554777425No Hit
GTGTGTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGAT719160.1307681208792318No Hit
GCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGACGACA718630.13067174857812217No Hit
CTCAACGCCTCACAACGCTTACACACCCTGCCTATCAACGTTCTAGTCTC715690.13013715505875936No Hit
CGGGCAATTTCCCAGGCATTACTCACCCGTCCGCCGCTCGCCACCCAAGG715030.13001714426869834No Hit
CGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGC714500.12992077196758872No Hit
CACCCGTCCGCCGCTCGCCACCCAAGGAACAAGTTCCTCTGTGCTGCCGC712250.12951164427419884No Hit
CTGATCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCTTA709090.12893704715814905No Hit
CGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA697740.12687322523816005No Hit
CTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGGCACAACCTCCAAGT688450.1251839824507858No Hit
GGGCTCGCTTCGCGCTTAGATGCTTTCAGCGCTTATCAATTCCGAACTTA687680.12504396986238125No Hit
CTGCTTCTTTTGCAGCCCACTCCCATGGTGTGACGGGCGGTGTGTACAAG687090.12493668748944792No Hit
TACGATTCCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCATGGCA682340.12407297347006928No Hit
CTCTGTATACGCCATTGTAGCACGTGTGTAGCCCTACTCGTAAGGGCCAT680310.1237038493733664No Hit
CCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATT679480.12355292671313813No Hit
CTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTA674820.12270557780149505No Hit
CGCCATTGTAGCACGTGTGTAGCCCTACTCGTAAGGGCCATGATGACTTG665210.12095814796439426No Hit
GGCACAACCTCCAAGTAGACATCGTTTACGGCGTGGACTACCAGGGTATC664220.12077813177930269No Hit
CCGACTTCATGGAGTCGAGTTGCAGACTCCAATCCGGACTACGACGTACT663020.1205599303428281No Hit
CTGGTATCTGCGACTCTCGTCAGCTCCATCCGCGAGGGACTTCACCATCA644980.11727963541449317No Hit
GGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTT644070.11711416599183327No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATATCACGTGT643210.11695778829569313No Hit
CGGTGCTTCTTCTGTAGGTAACGTCAAATGATTAAGGTATTAACTTAACC639920.11635955269069193No Hit
CACCGGATCACTATGACCTGCTTTCGCACCTGCTCGAACCGTCATTCTCG637280.11587950953044779No Hit
CCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCAC636960.11582132248072123No Hit
GCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGTAGGTAACGTCAA628210.11423027033976056No Hit
CCGGTTTCGGGTCTATATCCAGAGACTGAGCGCCCAGTTAAGACTCGCTT626620.1139411534364317No Hit
CCCAGGCGGTCTACTTAACGCGTTAGCTCCGAAAGCCACGCCTCTAGGGC624870.11362294300823955No Hit
GGTTAGCTCAACGCCTCACAACGCTTACACACCCTGCCTATCAACGTTCT622570.11320472358832989No Hit
TGTAGCCCTACTCGTAAGGGCCATGATGACTTGACGTCGTCCCCACCTTC619180.11258830453028913No Hit
CCACCTGCATGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCT617740.11232646280651962No Hit
GTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACA612740.11141729015454209No Hit
CTCCAAGTAGACATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTG609140.11076268584511825No Hit
CTGCTTTCGCACCTGCTCGAACCGTCATTCTCGCAGTTAAGCGGGCTTAT605050.11001898261580065No Hit
CTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCCGA602920.1096316750660582No Hit
CCGGGCAATTTCCCAGGCATTACTCACCCGTCCGCCGCTCGCCACCCAAG595360.10825700601626818No Hit
CGGCAGTCTCCCTGGAGTTCCCACCATTACGTGCTGGCAAACAAGGATAA592520.10774059594994495No Hit
CGTCGCCTTGGTGAGCCCTTACCTCACCAACTAGCTAATCCCACCTGGGC591820.10761331177866809No Hit
GCACGTGTGTAGCCCTACTCGTAAGGGCCATGATGACTTGACGTCGTCCC591700.10759149163502063No Hit
CTAAGTTAGAACATCAAACATACAAGGGTGGTATTTCAAGGACGGCTCCA589990.10728055458804434No Hit
CTCGGTTGATTTCTTCTCCTCGGGGTACTTAGATGTTTCAGTTCCCCCGG589330.1071605437979833No Hit
CTCCCTACTGAAAGTACTTTACAACCCGAAGGCCTTCTTCATACACGCGG579640.10539856719845085No Hit
CTCGCAGTTAAGCGGGCTTATGCCATTGCACTAACCTCACGATGTCCAAC576510.10482942511831292No Hit
CCCCCTCTACAGTACTCTAGCTTGTCAGTTTCAAATGCGATTCCTAGGTT576010.10473850785311514No Hit
CCAGTGTGGCTGATCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGT575570.10465850065974112No Hit
CTTCGCCACCGGTATTCCTTCAGATCTCTACGCATTTCACCGCTACACCT572670.10413118052159417No Hit
CGTGTGTAGCCCTACTCGTAAGGGCCATGATGACTTGACGTCGTCCCCAC570260.10369295930334099No Hit
GTCGCCTTGGTGAGCCCTTACCTCACCAACTAGCTAATCCCACCTGGGCA569220.10350385139172968No Hit
ACAGGTTAGCTCAACGCCTCACAACGCTTACACACCCTGCCTATCAACGT566070.10293107262098382No Hit
GTCTCAGTTCCAGTGTGGCTGATCATCCTCTCAGACCAGCTAGGGATCGT564250.102600133775664No Hit
AGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCA562840.10234374708780634No Hit
GTCTACTTAACGCGTTAGCTCCGAAAGCCACGCCTCTAGGGCACAACCTC554800.10088179746342647No Hit
TCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGT553200.10059086221479367No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)