FastQCFastQC Report
Wed 6 Sep 2023
6181_ctrl1_S1_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename6181_ctrl1_S1_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31800178
Sequences flagged as poor quality0
Sequence length200
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGTTCCAGTGTGGCTGATCATCCTCTCAGACCAGCTAGGGATCGTCG5400201.698166595168115No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGT3218731.0121735796573212No Hit
CTGGCAAACAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACAT2630390.8271620366401722No Hit
CCCCTCTACAGTACTCTAGCTTGTCAGTTTCAAATGCGATTCCTAGGTTG2548660.8014609226401185No Hit
GTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACT2385010.7499989465467772No Hit
CGGGCAATTAGTACAGGTTAGCTCAACGCCTCACAACGCTTACACACCCT2263680.7118450720621753No Hit
CCTAGCGCTACCTTGTTACGACTTCACCCCAGTCATGAACCACAAAGTGG2097240.6595057423892406No Hit
CCCCCCTCTACAGTACTCTAGCTTGTCAGTTTCAAATGCGATTCCTAGGT1980660.6228455702354874No Hit
CGTGTCTCAGTTCCAGTGTGGCTGATCATCCTCTCAGACCAGCTAGGGAT1760470.5536038194503188No Hit
TTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAG1363630.4288120651400128No Hit
CCGTGTCTCAGTTCCAGTGTGGCTGATCATCCTCTCAGACCAGCTAGGGA1314830.41346623908834723No Hit
CAACGTTCTAGTCTCGAACAACCCTTTAGGACAGTTAAACTGTCAGGGAA1289360.40545684995851283No Hit
CGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATT1203980.3786079436410702No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGATCATCCTCTCAGACCAGCT1170630.3681205809602701No Hit
CTCGAACAACCCTTTAGGACAGTTAAACTGTCAGGGAAGACTCATCTCAG1143700.3596520749034801No Hit
GGCTGATCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCT1109390.3488628271200243No Hit
ATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGA1101900.3465074943920125No Hit
CTCTACAGTACTCTAGCTTGTCAGTTTCAAATGCGATTCCTAGGTTGAGC1075070.33807043470008247No Hit
CCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTG1071060.3368094354691977No Hit
CCTAGCGCTACCTTGTTCCGACTTCACCCCAGTCATGAACCACAAAGTGG1046010.32893212107177516No Hit
GTTAGCTCAACGCCTCACAACGCTTACACACCCTGCCTATCAACGTTCTA1031570.32439126598599544No Hit
CACGTGTGTAGCCCTACTCGTAAGGGCCATGATGACTTGACGTCGTCCCC992920.3122372459676169No Hit
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC964040.3031555357960575No Hit
CTAGTCTCGAACAACCCTTTAGGACAGTTAAACTGTCAGGGAAGACTCAT951720.2992813436453092No Hit
CAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGC949930.29871845371431566No Hit
GGCAATTAGTACAGGTTAGCTCAACGCCTCACAACGCTTACACACCCTGC910570.2863411645054314No Hit
CTCACGGGCAATTAGTACAGGTTAGCTCAACGCCTCACAACGCTTACACA902310.2837436947679978No Hit
CGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCG899560.28287891973434864No Hit
GCTCGCTTCGCGCTTAGATGCTTTCAGCGCTTATCAATTCCGAACTTAGC881360.2771556813298341No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT872350.2743223638559507No Hit
TGGCAAACAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATT863030.2713915626510015No Hit
CCCAGGCGGTCTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGGC861200.27081609417406405No Hit
GTACGATTCCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCATGGC860630.2706368498943622No Hit
CCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGG856270.26926578838646753No Hit
CCTAGCGCTACCTTGTTTCGACTTCACCCCAGTCATGAACCACAAAGTGG840610.2643412876493962No Hit
CGATTCCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCATGGCATC839810.2640897167305164No Hit
CCTGCATGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCG834060.2622815507510681No Hit
GTACAGGTTAGCTCAACGCCTCACAACGCTTACACACCCTGCCTATCAAC827470.2602092353067961No Hit
GTCTCGAACAACCCTTTAGGACAGTTAAACTGTCAGGGAAGACTCATCTC822310.2585866028800216No Hit
GTCGACGTATAAGCCGGGTTCTGTTCCGCTTGCGCGGTAGTAGCCATTCC793200.24943256606928427No Hit
CAGGTTAGCTCAACGCCTCACAACGCTTACACACCCTGCCTATCAACGTT779010.24497032689565446No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATATCA767620.24138858593810386No Hit
GGGTACGATTCCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCATG748890.23549868179983144No Hit
CTCGCTTCGCGCTTAGATGCTTTCAGCGCTTATCAATTCCGAACTTAGCT744590.2341464881108527No Hit
CTGGAGTTCCCACCATTACGTGCTGGCAAACAAGGATAAGGGTTGCGCTC728180.22898613963733158No Hit
CTTTAGGACAGTTAAACTGTCAGGGAAGACTCATCTCAGGGCTCGCTTCG714840.22479119456501157No Hit
CTGGCACGGAGTTAGCCGGTGCTTCTTCTGTAGGTAACGTCAAATGATTA710210.22333522787199492No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGC699800.220061661290072No Hit
GTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGACG695580.21873462469298127No Hit
CCTGGAGTTCCCACCATTACGTGCTGGCAAACAAGGATAAGGGTTGCGCT687340.21614344422851975No Hit
CTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCTTACCTCACCAA681780.21439502634230537No Hit
CCCCAGGCGGTCTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGG676140.21262145136420305No Hit
CACGGAGTTAGCCGGTGCTTCTTCTGTAGGTAACGTCAAATGATTAAGGT674290.21203969361429362No Hit
GTCAGTATCTGTCCAGGGGGCCGCCTTCGCCACCGGTATTCCTTCAGATC667890.21002712626325548No Hit
GTCTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGGCACAACCTC650840.20466552105463057No Hit
CGCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTG645100.20286049971166828No Hit
CGTCGCCTTGGTGAGCCCTTACCTCACCAACTAGCTAATCCCACCTGGGC632780.19898630756091995No Hit
CTTGGTATTCTCTACCCGACCACCTGTGTCGGTTTGGGGTACGATTCCTG628860.1977536100584091No Hit
CCCTGGAGTTCCCACCATTACGTGCTGGCAAACAAGGATAAGGGTTGCGC624480.19637625927754243No Hit
CTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGGCACAACCTCCA604230.19000837039339843No Hit
GTCGCCTTGGTGAGCCCTTACCTCACCAACTAGCTAATCCCACCTGGGCA598220.1881184438653142No Hit
GGTTAGCTCAACGCCTCACAACGCTTACACACCCTGCCTATCAACGTTCT594650.18699580863981327No Hit
CTCAACGCCTCACAACGCTTACACACCCTGCCTATCAACGTTCTAGTCTC593470.18662474153446562No Hit
CACCTGCATGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTC589920.18550839558193669No Hit
GTGTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTA566630.1781845372060496No Hit
GGCTCGCTTCGCGCTTAGATGCTTTCAGCGCTTATCAATTCCGAACTTAG563310.17714051789269858No Hit
CTAGCGCTACCTTGTTACGACTTCACCCCAGTCATGAACCACAAAGTGGC561700.17663423141845305No Hit
GGCAAACAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTT557850.1754235463713442No Hit
ATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAG556690.17505876853896857No Hit
CTCCCCAGGCGGTCTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAG546910.17198331405566344No Hit
CCCTGCTTTGCTCTTGCGAGGTTATGCGGTATTAGCCATCGTTTCCAATG543690.17097074110717242No Hit
GGTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGAC539020.1695021958682118No Hit
AGGCGGTCTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGGCACA528880.1663135344714108No Hit
CGTCAATTCATTTGAGTTTTAATCTTGCGACCGTACTCCCCAGGCGGTCT522150.16419719411633482No Hit
TGATCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCTTAC520100.16355254363670543No Hit
CCCTAGCGCTACCTTGTTACGACTTCACCCCAGTCATGAACCACAAAGTG517830.1628387111543841No Hit
ACAGGTTAGCTCAACGCCTCACAACGCTTACACACCCTGCCTATCAACGT516630.16246135477606446No Hit
CCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTT507250.15951168575219926No Hit
CTGCCTATCAACGTTCTAGTCTCGAACAACCCTTTAGGACAGTTAAACTG503980.15848338962127823No Hit
GGGCAATTAGTACAGGTTAGCTCAACGCCTCACAACGCTTACACACCCTG502090.15788905332542477No Hit
ATGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTA495840.15592365552167664No Hit
TACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTA493670.1552412694042153No Hit
CCCACCTTCGTGCTCCTCCGTTACTCTTTGGGAGGAGACCGCCCCAGTCA491730.15463120992593185No Hit
GTGGAGTCGACGTATAAGCCGGGTTCTGTTCCGCTTGCGCGGTAGTAGCC488820.1537161207085067No Hit
GCTGATCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCTT485800.15276644048973562No Hit
CTCCAATCCGGACTACGACGTACTTTGTGAGATTCGCTCCACCTCGCGGT469110.14751804219460657No Hit
CAGGGCTCGCTTCGCGCTTAGATGCTTTCAGCGCTTATCAATTCCGAACT461970.1452727717436047No Hit
CAGGCGGTCTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGGCAC450250.1415872577820162No Hit
CCAGTGTGGCTGATCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGT439130.1380904220095875No Hit
CGCGTTAGCTCCGAAAGCCACACCTCTAGGGCACAACCTCCAAGTAGACA435940.13708728297055445No Hit
TAGCTCAACGCCTCACAACGCTTACACACCCTGCCTATCAACGTTCTAGT435850.13705898124218047No Hit
CTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACC434210.13654326085847696No Hit
CCTTGGTATTCTCTACCCGACCACCTGTGTCGGTTTGGGGTACGATTCCT432220.13591747819776356No Hit
CTGATCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCTTA431880.13581056055723967No Hit
CCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTG431280.13562188236807982No Hit
GGGCTCGCTTCGCGCTTAGATGCTTTCAGCGCTTATCAATTCCGAACTTA425430.13378227002377158No Hit
CTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGGCACAACCTCCAAGT425160.13369736483864963No Hit
CCAGGCGGTCTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGGCA418110.13148039611602175No Hit
GCAGGTTAGCTCAACGCCTCACAACGCTTACACACCCTGCCTATCAACGT417050.13114706464850606No Hit
CTGGCGATGTTCTACTCTCACATGGGGAGACCCCACACTACCATCGACGC416270.13090178300259828No Hit
TACGATTCCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCATGGCA411890.12952443222173157No Hit
CGGGCAATTTCCCAGGCATTACTCACCCGTCCGCCGCTCGCCACCCAAGG411540.1294143699447217No Hit
GTTCTAGTCTCGAACAACCCTTTAGGACAGTTAAACTGTCAGGGAAGACT410110.12896468692722413No Hit
CACCCGTCCGCCGCTCGCCACCCAAGGAACAAGTTCCTCTGTGCTGCCGC407770.12822884198950082No Hit
CTCACCAACTAGCTAATCCCACCTGGGCATATCCGGTAGCGCAAGGCCCG407580.12816909389626688No Hit
GGAAGACTCATCTCAGGGCTCGCTTCGCGCTTAGATGCTTTCAGCGCTTA404880.12732004204504765No Hit
CCCGGTTTCGGGTCTATATCCAGAGACTGAGCGCCCAGTTAAGACTCGCT402520.12657790783435238No Hit
CGACGTATAAGCCGGGTTCTGTTCCGCTTGCGCGGTAGTAGCCATTCCTC393680.123798049180731No Hit
CCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATT393650.12378861527127302No Hit
CTCCGTTACTCTTTGGGAGGAGACCGCCCCAGTCAAACTACCCACCAGGC393280.12367226372129111No Hit
CGACCGTACTCCCCAGGCGGTCTACTTAACGCGTTAGCTCCGAAAGCCAC393250.12366282981183313No Hit
GGCACAACCTCCAAGTAGACATCGTTTACGGCGTGGACTACCAGGGTATC387670.12190812265264678No Hit
CTCGCAGTTAAGCGGGCTTATGCCATTGCACTAACCTCACGATGTCCAAC385740.12130120781084937No Hit
CTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGACGACAGCC384900.12103705834602561No Hit
CGGTGCTTCTTCTGTAGGTAACGTCAAATGATTAAGGTATTAACTTAACC382360.12023832067858237No Hit
CTGAAATTCTACCCCCCTCTACAGTACTCTAGCTTGTCAGTTTCAAATGC381810.12006536567185254No Hit
GGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTG381740.12004335321645054No Hit
CCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCATGGCATCAATGA380680.11971002174893486No Hit
GGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTCC375090.11795216995326252No Hit
GCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGTAGGTAACGTCAA374330.11771317758032675No Hit
TCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGTA371670.11687670427505154No Hit
CCCACCATTACGTGCTGGCAAACAAGGATAAGGGTTGCGCTCGTTGCGGG370790.1165999762642838No Hit
ACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAG370140.11639557489269399No Hit
CGCTACCTTGTTACGACTTCACCCCAGTCATGAACCACAAAGTGGCAAGC366920.11538300194420296No Hit
CTACAGTACTCTAGCTTGTCAGTTTCAAATGCGATTCCTAGGTTGAGCCC364500.11462199991459168No Hit
GTCTCAGTTCCAGTGTGGCTGATCATCCTCTCAGACCAGCTAGGGATCGT364030.11447420199974981No Hit
CGGCAGTCTCCCTGGAGTTCCCACCATTACGTGCTGGCAAACAAGGATAA357740.11249622565005768No Hit
GGAGTCGACGTATAAGCCGGGTTCTGTTCCGCTTGCGCGGTAGTAGCCAT356790.11219748518388796No Hit
CGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGC356640.11215031563659801No Hit
GGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTT350230.1101346036490739No Hit
CCGGGCAATTTCCCAGGCATTACTCACCCGTCCGCCGCTCGCCACCCAAG350140.11010630192069995No Hit
CTAGCTAATCCCACCTGGGCATATCCGGTAGCGCAAGGCCCGAAGGTCCC349610.10993963618694211No Hit
GGTGTGTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGA348440.10957171371808044No Hit
CGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA346320.10890505078304909No Hit
CCCATATTCAGACAGGATATCACGTGTCCCGCCCTACTCGATTTCACGCT343980.10816920584532576No Hit
GTTTTAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTAACGCGTTAGC341600.10742078236165849No Hit
GTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCA339650.10680757824688906No Hit
ATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCTTACCTCAC339330.10670694987933715No Hit
CTGCTTCTTTTGCAGCCCACTCCCATGGTGTGACGGGCGGTGTGTACAAG339160.10665349105907519No Hit
CCCCTAGCGCTACCTTGTTACGACTTCACCCCAGTCATGAACCACAAAGT338580.10647110214288738No Hit
CTGGGCATATCCGGTAGCGCAAGGCCCGAAGGTCCCCTGCTTTGCTCTTG337050.10598997276052982No Hit
GCACGGAGTTAGCCGGTGCTTCTTCTGTAGGTAACGTCAAATGATTAAGG336140.1057038108403041No Hit
GTCTACTTAACGCGTTAGCTCCGAAAGCCACGCCTCTAGGGCACAACCTC336010.10566293056598615No Hit
CTCCAAGTAGACATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTG328680.10335791202175032No Hit
CCACCTGCATGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCT328170.10319753556096446No Hit
GGGCAATTTCCCAGGCATTACTCACCCGTCCGCCGCTCGCCACCCAAGGA327400.1029553985515427No Hit
GGTCTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGGCACAACCT326020.1025214387164751No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)