FastQCFastQC Report
Wed 6 Sep 2023
6186_SSV3_S6_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename6186_SSV3_S6_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30927539
Sequences flagged as poor quality0
Sequence length200
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGTTCCAGTGTGGCTGATCATCCTCTCAGACCAGCTAGGGATCGTCG5422201.7531947821648532No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGT2589550.837295848208291No Hit
CCCCTCTACAGTACTCTAGCTTGTCAGTTTCAAATGCGATTCCTAGGTTG2556560.8266289794348008No Hit
GTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACT2388900.7724183938463387No Hit
CTGGCAAACAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACAT2224850.7193750527644635No Hit
CGGGCAATTAGTACAGGTTAGCTCAACGCCTCACAACGCTTACACACCCT2048430.6623320400630649No Hit
CGTGTCTCAGTTCCAGTGTGGCTGATCATCCTCTCAGACCAGCTAGGGAT1981790.6407849004733289No Hit
CCCCCCTCTACAGTACTCTAGCTTGTCAGTTTCAAATGCGATTCCTAGGT1967000.6360027546970355No Hit
CCTAGCGCTACCTTGTTACGACTTCACCCCAGTCATGAACCACAAAGTGG1960620.6339398682837325No Hit
CCGTGTCTCAGTTCCAGTGTGGCTGATCATCCTCTCAGACCAGCTAGGGA1515440.4899969570808722No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGATCATCCTCTCAGACCAGCT1220090.39449954294779166No Hit
TTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAG1212200.3919484185275783No Hit
CAACGTTCTAGTCTCGAACAACCCTTTAGGACAGTTAAACTGTCAGGGAA1184290.38292409881044853No Hit
CGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATT1137370.3677531535891039No Hit
GGCTGATCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCT1066750.34491913501426674No Hit
CTCTACAGTACTCTAGCTTGTCAGTTTCAAATGCGATTCCTAGGTTGAGC1035380.33477607125481273No Hit
CACGTGTGTAGCCCTACTCGTAAGGGCCATGATGACTTGACGTCGTCCCC1021610.3303237286355051No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT1016300.32860681220060867No Hit
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC1011690.3271162312656044No Hit
CCTAGCGCTACCTTGTTCCGACTTCACCCCAGTCATGAACCACAAAGTGG974460.3150784160356244No Hit
CGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCG971070.31398230554328943No Hit
CTCGAACAACCCTTTAGGACAGTTAAACTGTCAGGGAAGACTCATCTCAG964250.31177715110148274No Hit
CAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGC957720.30966576422391706No Hit
CCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTG946550.3060540963184947No Hit
ATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGA911430.2946985209524754No Hit
GTTAGCTCAACGCCTCACAACGCTTACACACCCTGCCTATCAACGTTCTA904480.2924513327749744No Hit
GGCAATTAGTACAGGTTAGCTCAACGCCTCACAACGCTTACACACCCTGC841920.2722234058131816No Hit
GTCGACGTATAAGCCGGGTTCTGTTCCGCTTGCGCGGTAGTAGCCATTCC838460.27110466177085735No Hit
CTAGTCTCGAACAACCCTTTAGGACAGTTAAACTGTCAGGGAAGACTCAT786810.2544043352430984No Hit
CCTAGCGCTACCTTGTTTCGACTTCACCCCAGTCATGAACCACAAAGTGG785890.25410686572895436No Hit
CCTGCATGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCG775980.2509026017233379No Hit
GCTCGCTTCGCGCTTAGATGCTTTCAGCGCTTATCAATTCCGAACTTAGC769760.24889144913858163No Hit
CCCAGGCGGTCTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGGC764890.24731680073218887No Hit
GTACAGGTTAGCTCAACGCCTCACAACGCTTACACACCCTGCCTATCAAC743850.24051380227828664No Hit
TGGCAAACAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATT738680.2388421529433687No Hit
CGATTCCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCATGGCATC736310.23807584560801948No Hit
CCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGG733300.23710260295848307No Hit
CTCACGGGCAATTAGTACAGGTTAGCTCAACGCCTCACAACGCTTACACA710260.22965293164774603No Hit
CTGGCACGGAGTTAGCCGGTGCTTCTTCTGTAGGTAACGTCAAATGATTA708290.22901595888376375No Hit
GTCAGTATCTGTCCAGGGGGCCGCCTTCGCCACCGGTATTCCTTCAGATC700100.2263678335350252No Hit
CGCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTG698470.22584079515670483No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATATCA697320.22546895826402483No Hit
GTACGATTCCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCATGGC696370.2251617886570283No Hit
CAGGTTAGCTCAACGCCTCACAACGCTTACACACCCTGCCTATCAACGTT694770.22464445037156047No Hit
CTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCTTACCTCACCAA679980.21986230459526704No Hit
GTCTCGAACAACCCTTTAGGACAGTTAAACTGTCAGGGAAGACTCATCTC675070.21827472273173754No Hit
CTCGCTTCGCGCTTAGATGCTTTCAGCGCTTATCAATTCCGAACTTAGCT668770.2162377032327079No Hit
GGGTACGATTCCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCATG658040.21276830335578917No Hit
CACGGAGTTAGCCGGTGCTTCTTCTGTAGGTAACGTCAAATGATTAAGGT633500.20483362740242603No Hit
CTTTAGGACAGTTAAACTGTCAGGGAAGACTCATCTCAGGGCTCGCTTCG616150.19922374036938406No Hit
CTGGAGTTCCCACCATTACGTGCTGGCAAACAAGGATAAGGGTTGCGCTC611400.19768789233440137No Hit
CCCCAGGCGGTCTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGG598680.193575052964932No Hit
CCTGGAGTTCCCACCATTACGTGCTGGCAAACAAGGATAAGGGTTGCGCT590390.1908945939733517No Hit
CGTCGCCTTGGTGAGCCCTTACCTCACCAACTAGCTAATCCCACCTGGGC585460.1893005453812539No Hit
GTGTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTA579830.18748016128926392No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGC573920.18556924299731706No Hit
CACCTGCATGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTC561640.18159867165635132No Hit
GGTTAGCTCAACGCCTCACAACGCTTACACACCCTGCCTATCAACGTTCT552950.17878887809340405No Hit
CTCAACGCCTCACAACGCTTACACACCCTGCCTATCAACGTTCTAGTCTC547890.177152795765612No Hit
CTTGGTATTCTCTACCCGACCACCTGTGTCGGTTTGGGGTACGATTCCTG545470.1763703216088419No Hit
CCCTGGAGTTCCCACCATTACGTGCTGGCAAACAAGGATAAGGGTTGCGC544220.17596615107332012No Hit
TGATCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCTTAC543960.1758820836019316No Hit
GTCGCCTTGGTGAGCCCTTACCTCACCAACTAGCTAATCCCACCTGGGCA540070.17462430489538788No Hit
CCCACCTTCGTGCTCCTCCGTTACTCTTTGGGAGGAGACCGCCCCAGTCA539410.17441090285263242No Hit
GTCTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGGCACAACCTC537570.1738159638243444No Hit
GTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGACG531790.17194707926809177No Hit
CGTCAATTCATTTGAGTTTTAATCTTGCGACCGTACTCCCCAGGCGGTCT527370.17051793225448683No Hit
CTAGCGCTACCTTGTTACGACTTCACCCCAGTCATGAACCACAAAGTGGC522850.16905645159804017No Hit
GTGGAGTCGACGTATAAGCCGGGTTCTGTTCCGCTTGCGCGGTAGTAGCC515210.1665861612849312No Hit
CCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTG511330.16533161594267168No Hit
CTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGGCACAACCTCCA506030.16361793287205945No Hit
TACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTA503090.16266732377251225No Hit
CCCTGCTTTGCTCTTGCGAGGTTATGCGGTATTAGCCATCGTTTCCAATG485800.15707683692517532No Hit
GCTGATCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCTT481010.15552805543305595No Hit
GGCAAACAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTT478750.15479731510483263No Hit
GGGCAATTAGTACAGGTTAGCTCAACGCCTCACAACGCTTACACACCCTG474970.15357510340541483No Hit
ACAGGTTAGCTCAACGCCTCACAACGCTTACACACCCTGCCTATCAACGT473260.15302219811282106No Hit
CCCTAGCGCTACCTTGTTACGACTTCACCCCAGTCATGAACCACAAAGTG469380.15176765277056153No Hit
AGGCGGTCTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGGCACA468090.15135054877790308No Hit
CTGCCTATCAACGTTCTAGTCTCGAACAACCCTTTAGGACAGTTAAACTG458340.1481980186008334No Hit
ATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAG455190.14717950885131856No Hit
CTCCCCAGGCGGTCTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAG454360.1469111396157321No Hit
GGCTCGCTTCGCGCTTAGATGCTTTCAGCGCTTATCAATTCCGAACTTAG448850.14512955589515222No Hit
CCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTT445370.14400434512425964No Hit
CTGATCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCTTA431970.1396716369834664No Hit
CCAGTGTGGCTGATCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGT431040.13937093410503823No Hit
CTCCAATCCGGACTACGACGTACTTTGTGAGATTCGCTCCACCTCGCGGT429890.13899909721235823No Hit
CGACGTATAAGCCGGGTTCTGTTCCGCTTGCGCGGTAGTAGCCATTCCTC412200.1332792757936543No Hit
GGTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGAC408640.13212819810848836No Hit
TAGCTCAACGCCTCACAACGCTTACACACCCTGCCTATCAACGTTCTAGT399660.12922463698130007No Hit
GGAGTCGACGTATAAGCCGGGTTCTGTTCCGCTTGCGCGGTAGTAGCCAT396370.1281608601318068No Hit
CCTTGGTATTCTCTACCCGACCACCTGTGTCGGTTTGGGGTACGATTCCT394650.12760472147492888No Hit
CTCACCAACTAGCTAATCCCACCTGGGCATATCCGGTAGCGCAAGGCCCG393920.12736868588218417No Hit
CAGGCGGTCTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGGCAC392720.12698068216808328No Hit
GGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTCC389380.12590073849716915No Hit
CAGGGCTCGCTTCGCGCTTAGATGCTTTCAGCGCTTATCAATTCCGAACT389200.125842537940054No Hit
CTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACC387290.12522496536177677No Hit
GCAGGTTAGCTCAACGCCTCACAACGCTTACACACCCTGCCTATCAACGT386180.12486606192623345No Hit
ACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAG382010.12351774901973288No Hit
CGCGTTAGCTCCGAAAGCCACACCTCTAGGGCACAACCTCCAAGTAGACA381360.12330758034126155No Hit
CTGGCGATGTTCTACTCTCACATGGGGAGACCCCACACTACCATCGACGC380990.1231879458627471No Hit
CGGTGCTTCTTCTGTAGGTAACGTCAAATGATTAAGGTATTAACTTAACC378020.12222763667034742No Hit
GTCTCAGTTCCAGTGTGGCTGATCATCCTCTCAGACCAGCTAGGGATCGT377390.12202393472044445No Hit
CTCCGTTACTCTTTGGGAGGAGACCGCCCCAGTCAAACTACCCACCAGGC373800.12086315694242597No Hit
GCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGTAGGTAACGTCAA372160.12033288519982142No Hit
CCAGGCGGTCTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGGCA370840.11990608111431046No Hit
CCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATT369740.11955041104305131No Hit
GGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTG369320.119414609743116No Hit
CTACAGTACTCTAGCTTGTCAGTTTCAAATGCGATTCCTAGGTTGAGCCC368780.11924000807177058No Hit
ATGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTA367930.11896517210761581No Hit
GGTGTGTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGA367360.11878087034341787No Hit
GGGCTCGCTTCGCGCTTAGATGCTTTCAGCGCTTATCAATTCCGAACTTA362280.11713832128705746No Hit
CTCGCAGTTAAGCGGGCTTATGCCATTGCACTAACCTCACGATGTCCAAC360660.11661451627302127No Hit
TACGATTCCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCATGGCA357370.11555073942352802No Hit
GTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCA354960.11477149863104207No Hit
CGACCGTACTCCCCAGGCGGTCTACTTAACGCGTTAGCTCCGAAAGCCAC353890.11442552865263543No Hit
CTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGGCACAACCTCCAAGT352170.11386938999575752No Hit
CTGCTTCTTTTGCAGCCCACTCCCATGGTGTGACGGGCGGTGTGTACAAG344010.11123096473987149No Hit
GGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTT342820.11084619439005476No Hit
GTTCTAGTCTCGAACAACCCTTTAGGACAGTTAAACTGTCAGGGAAGACT342820.11084619439005476No Hit
GGCACAACCTCCAAGTAGACATCGTTTACGGCGTGGACTACCAGGGTATC342390.11070715972583528No Hit
ATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCTTACCTCAC341940.11056165833304744No Hit
CCTCTGTATACGCCATTGTAGCACGTGTGTAGCCCTACTCGTAAGGGCCA336350.10875420769819416No Hit
CTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGACGACAGCC334560.10817543549132701No Hit
CGCTACCTTGTTACGACTTCACCCCAGTCATGAACCACAAAGTGGCAAGC334100.108026700734255No Hit
CTGAAATTCTACCCCCCTCTACAGTACTCTAGCTTGTCAGTTTCAAATGC332610.10754492945591305No Hit
CGGCAGTCTCCCTGGAGTTCCCACCATTACGTGCTGGCAAACAAGGATAA331370.10714399228467547No Hit
GGAAGACTCATCTCAGGGCTCGCTTCGCGCTTAGATGCTTTCAGCGCTTA321630.10399469547188996No Hit
GCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGC319810.10340622317217027No Hit
CGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGC317820.10276278367961965No Hit
TCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGTA317590.10268841630108365No Hit
CGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA316750.10241681370121301No Hit
CTAGCTAATCCCACCTGGGCATATCCGGTAGCGCAAGGCCCGAAGGTCCC313840.10147590469451837No Hit
GCACGGAGTTAGCCGGTGCTTCTTCTGTAGGTAACGTCAAATGATTAAGG312870.10116226835895348No Hit
GCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGC310860.1005123621378345No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)