FastQCFastQC Report
Wed 6 Sep 2023
6185_SSV2_S5_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename6185_SSV2_S5_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18710994
Sequences flagged as poor quality0
Sequence length200
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGTTCCAGTGTGGCTGATCATCCTCTCAGACCAGCTAGGGATCGTCG2973171.588996287423319No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGT1872391.0006897549109364No Hit
CGGGCAATTAGTACAGGTTAGCTCAACGCCTCACAACGCTTACACACCCT1410070.7536050730388776No Hit
CTGGCAAACAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACAT1313790.7021486939710418No Hit
CCCCTCTACAGTACTCTAGCTTGTCAGTTTCAAATGCGATTCCTAGGTTG1251100.6686443275007196No Hit
CCTAGCGCTACCTTGTTACGACTTCACCCCAGTCATGAACCACAAAGTGG1070580.572166288974279No Hit
CGTGTCTCAGTTCCAGTGTGGCTGATCATCCTCTCAGACCAGCTAGGGAT1051300.5618621864771054No Hit
CCCCCCTCTACAGTACTCTAGCTTGTCAGTTTCAAATGCGATTCCTAGGT1033110.5521406291937243No Hit
GTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACT1026390.5485491577839211No Hit
CCGTGTCTCAGTTCCAGTGTGGCTGATCATCCTCTCAGACCAGCTAGGGA805020.43023903486901877No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGATCATCCTCTCAGACCAGCT768210.41056610888764117No Hit
GGCTGATCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCT734300.39244307384204175No Hit
TTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAG686680.3669927957862634No Hit
CAACGTTCTAGTCTCGAACAACCCTTTAGGACAGTTAAACTGTCAGGGAA627570.33540174295390185No Hit
GTCGACGTATAAGCCGGGTTCTGTTCCGCTTGCGCGGTAGTAGCCATTCC612210.3271926654457802No Hit
CGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATT606810.32430666163433114No Hit
GTTAGCTCAACGCCTCACAACGCTTACACACCCTGCCTATCAACGTTCTA590960.31583570600257793No Hit
CTCACGGGCAATTAGTACAGGTTAGCTCAACGCCTCACAACGCTTACACA578520.3091872083332398No Hit
CCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTG571170.3052590364787675No Hit
ATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGA566640.30283799994805194No Hit
GTGGAGTCGACGTATAAGCCGGGTTCTGTTCCGCTTGCGCGGTAGTAGCC552880.29548403468035955No Hit
CTCGAACAACCCTTTAGGACAGTTAAACTGTCAGGGAAGACTCATCTCAG551970.2949976895936154No Hit
CCTGCATGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCG542680.2900326941476225No Hit
GGCAATTAGTACAGGTTAGCTCAACGCCTCACAACGCTTACACACCCTGC536160.286548111767873No Hit
CCTAGCGCTACCTTGTTCCGACTTCACCCCAGTCATGAACCACAAAGTGG530560.2835552189263702No Hit
CCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGG504000.26936035573524314No Hit
CTAGTCTCGAACAACCCTTTAGGACAGTTAAACTGTCAGGGAAGACTCAT479400.25621300503864197No Hit
GTACAGGTTAGCTCAACGCCTCACAACGCTTACACACCCTGCCTATCAAC471950.25223138866914285No Hit
CTCTACAGTACTCTAGCTTGTCAGTTTCAAATGCGATTCCTAGGTTGAGC467700.2499599967805024No Hit
CAGGTTAGCTCAACGCCTCACAACGCTTACACACCCTGCCTATCAACGTT460980.24636852537069917No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT459580.2456203021603235No Hit
CCCAGGCGGTCTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGGC434560.23224848450060964No Hit
TGGCAAACAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATT431480.23060239343778316No Hit
CGTCGCCTTGGTGAGCCCTTACCTCACCAACTAGCTAATCCCACCTGGGC430850.23026569299311409No Hit
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC430510.23008398164202284No Hit
GTCTCGAACAACCCTTTAGGACAGTTAAACTGTCAGGGAAGACTCATCTC427730.2285982241242769No Hit
CCTAGCGCTACCTTGTTTCGACTTCACCCCAGTCATGAACCACAAAGTGG424450.22684524403139675No Hit
CTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCTTACCTCACCAA416590.22264450515028758No Hit
CCTGGAGTTCCCACCATTACGTGCTGGCAAACAAGGATAAGGGTTGCGCT412160.22027691313459882No Hit
GTCAGTATCTGTCCAGGGGGCCGCCTTCGCCACCGGTATTCCTTCAGATC400980.2143018163545988No Hit
CTGGAGTTCCCACCATTACGTGCTGGCAAACAAGGATAAGGGTTGCGCTC394480.21082792287785457No Hit
GGTTAGCTCAACGCCTCACAACGCTTACACACCCTGCCTATCAACGTTCT392800.20993005502540377No Hit
CACGTGTGTAGCCCTACTCGTAAGGGCCATGATGACTTGACGTCGTCCCC390590.20874893124331076No Hit
CTGGCACGGAGTTAGCCGGTGCTTCTTCTGTAGGTAACGTCAAATGATTA390200.20854049763470608No Hit
GCTCGCTTCGCGCTTAGATGCTTTCAGCGCTTATCAATTCCGAACTTAGC387800.20725782927406206No Hit
CCCTGGAGTTCCCACCATTACGTGCTGGCAAACAAGGATAAGGGTTGCGC387030.20684630650835545No Hit
GTCGCCTTGGTGAGCCCTTACCTCACCAACTAGCTAATCCCACCTGGGCA384850.20568121608077047No Hit
CGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCG384210.20533917118459874No Hit
CACCTGCATGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTC382960.20467111474676333No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGC382710.20453750345919625No Hit
CCCCAGGCGGTCTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGG377140.20156064397220158No Hit
CTTTAGGACAGTTAAACTGTCAGGGAAGACTCATCTCAGGGCTCGCTTCG376640.2012934213970674No Hit
CACGGAGTTAGCCGGTGCTTCTTCTGTAGGTAACGTCAAATGATTAAGGT369880.19768057218125346No Hit
GGAGTCGACGTATAAGCCGGGTTCTGTTCCGCTTGCGCGGTAGTAGCCAT365420.19529694681105664No Hit
GTCTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGGCACAACCTC360670.192758332347282No Hit
CTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGGCACAACCTCCA354590.18950890583365054No Hit
ATGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTA350430.18728561400853425No Hit
ACAGGTTAGCTCAACGCCTCACAACGCTTACACACCCTGCCTATCAACGT346490.18517990011647698No Hit
CAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGC345820.18482182186579718No Hit
GGGCAATTAGTACAGGTTAGCTCAACGCCTCACAACGCTTACACACCCTG344110.18390792065883832No Hit
GTACGATTCCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCATGGC342130.18284971926130703No Hit
GGGTACGATTCCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCATG333990.17849933573812274No Hit
CGCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTG326910.1747154640742229No Hit
CTGGCGATGTTCTACTCTCACATGGGGAGACCCCACACTACCATCGACGC325110.17375346280373988No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATATCA316810.16931756805651266No Hit
CTCGCTTCGCGCTTAGATGCTTTCAGCGCTTATCAATTCCGAACTTAGCT316220.16900224541785433No Hit
TGATCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCTTAC315330.1685265892341155No Hit
GCTGATCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCTT310410.1658971190947953No Hit
CTGCCTATCAACGTTCTAGTCTCGAACAACCCTTTAGGACAGTTAAACTG306740.16393570539331048No Hit
CCCTAGCGCTACCTTGTTACGACTTCACCCCAGTCATGAACCACAAAGTG304350.16265838148416917No Hit
CCAGTGTGGCTGATCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGT303980.16246063677856987No Hit
CGATTCCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCATGGCATC300410.1605526675921119No Hit
GTGTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTA292390.1562664174869598No Hit
CTCCCCAGGCGGTCTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAG286410.15307043548835514No Hit
CCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTT284480.15203895634833725No Hit
CTCAACGCCTCACAACGCTTACACACCCTGCCTATCAACGTTCTAGTCTC282410.15093265488728178No Hit
CTCACCAACTAGCTAATCCCACCTGGGCATATCCGGTAGCGCAAGGCCCG271770.14524615848842665No Hit
GTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGACG271070.1448720468832388No Hit
GCAGGTTAGCTCAACGCCTCACAACGCTTACACACCCTGCCTATCAACGT267220.1428144330547057No Hit
GGCAAACAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTT266620.14249376596454472No Hit
CCCTGCTTTGCTCTTGCGAGGTTATGCGGTATTAGCCATCGTTTCCAATG265060.14166003153012607No Hit
CGACGTATAAGCCGGGTTCTGTTCCGCTTGCGCGGTAGTAGCCATTCCTC263330.14073544142016184No Hit
GTCTCAGTTCCAGTGTGGCTGATCATCCTCTCAGACCAGCTAGGGATCGT256410.13703708098030495No Hit
CTAGCGCTACCTTGTTACGACTTCACCCCAGTCATGAACCACAAAGTGGC254650.13609645751583266No Hit
AGGCGGTCTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGGCACA254600.13606973525831925No Hit
CTGATCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCTTA253620.13554597901105628No Hit
CTTGGTATTCTCTACCCGACCACCTGTGTCGGTTTGGGGTACGATTCCTG252970.13519858966338186No Hit
GGCTCGCTTCGCGCTTAGATGCTTTCAGCGCTTATCAATTCCGAACTTAG250880.134081599299321No Hit
CTGAAATTCTACCCCCCTCTACAGTACTCTAGCTTGTCAGTTTCAAATGC250580.13392126575424051No Hit
CTGGGCATATCCGGTAGCGCAAGGCCCGAAGGTCCCCTGCTTTGCTCTTG242760.12974190467914212No Hit
GTTCTAGTCTCGAACAACCCTTTAGGACAGTTAAACTGTCAGGGAAGACT240000.12826683606440148No Hit
CCCACCATTACGTGCTGGCAAACAAGGATAAGGGTTGCGCTCGTTGCGGG236830.12657264493805084No Hit
TAGCTCAACGCCTCACAACGCTTACACACCCTGCCTATCAACGTTCTAGT235950.12610233320581474No Hit
ATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCTTACCTCAC235720.125979410821253No Hit
CTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGGCACAACCTCCAAGT231630.12379353015665548No Hit
GTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGATCATCCTCTCAGACCAG231310.12362250770856963No Hit
CCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTG228040.12187487206719216No Hit
CTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACC225270.12039445900094885No Hit
GGCACAACCTCCAAGTAGACATCGTTTACGGCGTGGACTACCAGGGTATC223350.11936832431243365No Hit
CTAGCTAATCCCACCTGGGCATATCCGGTAGCGCAAGGCCCGAAGGTCCC223210.11929350199139607No Hit
GGTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGAC221740.11850786762050163No Hit
ATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAG220810.11801083363075207No Hit
CCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATT219580.11735346609592201No Hit
TCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGTA219310.11720916590534955No Hit
CAGGGCTCGCTTCGCGCTTAGATGCTTTCAGCGCTTATCAATTCCGAACT216960.11595321980221895No Hit
GCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACC216560.1157394417421116No Hit
CCAGGCGGTCTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGGCA215950.11541343020044793No Hit
CTCCAATCCGGACTACGACGTACTTTGTGAGATTCGCTCCACCTCGCGGT213670.1141948952578361No Hit
CAGGCGGTCTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGGCAC213500.11410403958229048No Hit
CCACCTGCATGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCT213390.11404525061576098No Hit
CGCGTTAGCTCCGAAAGCCACACCTCTAGGGCACAACCTCCAAGTAGACA212540.1135909722380329No Hit
CGCTACCTTGTTACGACTTCACCCCAGTCATGAACCACAAAGTGGCAAGC212040.11332374966289871No Hit
GGGCTCGCTTCGCGCTTAGATGCTTTCAGCGCTTATCAATTCCGAACTTA211840.11321686063284506No Hit
CGACCGTACTCCCCAGGCGGTCTACTTAACGCGTTAGCTCCGAAAGCCAC211340.1129496380577109No Hit
GGAAGACTCATCTCAGGGCTCGCTTCGCGCTTAGATGCTTTCAGCGCTTA207360.11082254635964288No Hit
CCCACCTTCGTGCTCCTCCGTTACTCTTTGGGAGGAGACCGCCCCAGTCA204260.10916576639381104No Hit
TACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTA202620.10828927634737096No Hit
CCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCTTACCTCACCA200790.10731124172237991No Hit
CCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTG198770.10623166251883785No Hit
GCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGTAGGTAACGTCAA197510.10555826162949974No Hit
CGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGC197410.1055048171144729No Hit
GGTCTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGGCACAACCT197190.10538723918141388No Hit
CCCCTAGCGCTACCTTGTTACGACTTCACCCCAGTCATGAACCACAAAGT196610.10507726099425825No Hit
CGGTGCTTCTTCTGTAGGTAACGTCAAATGATTAAGGTATTAACTTAACC194770.10409388191776449No Hit
GCGCTACCTTGTTACGACTTCACCCCAGTCATGAACCACAAAGTGGCAAG194110.1037411481185874No Hit
CGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA192820.10305171387474123No Hit
CGTCAATTCATTTGAGTTTTAATCTTGCGACCGTACTCCCCAGGCGGTCT192380.10281655800862316No Hit
GCACGGAGTTAGCCGGTGCTTCTTCTGTAGGTAACGTCAAATGATTAAGG192100.10266691336654804No Hit
GGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTT191900.10256002433649436No Hit
GGTGTGTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGA191480.10233555737338167No Hit
CTGCATGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGT189450.10125063371833694No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)