FastQCFastQC Report
Wed 6 Sep 2023
6189_NO3_S9_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename6189_NO3_S9_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23996675
Sequences flagged as poor quality0
Sequence length200
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGTTCCAGTGTGGCTGATCATCCTCTCAGACCAGCTAGGGATCGTCG3479721.4500842304194228No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGT2174420.9061338706299935No Hit
CGGGCAATTAGTACAGGTTAGCTCAACGCCTCACAACGCTTACACACCCT1859820.775032374276853No Hit
CCCCTCTACAGTACTCTAGCTTGTCAGTTTCAAATGCGATTCCTAGGTTG1801900.7508956970080229No Hit
GTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACT1712900.7138072253760157No Hit
CTGGCAAACAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACAT1606750.6695719302778406No Hit
CCCCCCTCTACAGTACTCTAGCTTGTCAGTTTCAAATGCGATTCCTAGGT1483350.6181481392734618No Hit
CCTAGCGCTACCTTGTTACGACTTCACCCCAGTCATGAACCACAAAGTGG1376790.5737419871711393No Hit
CGTGTCTCAGTTCCAGTGTGGCTGATCATCCTCTCAGACCAGCTAGGGAT1351700.5632863719661162No Hit
TTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAG1150090.4792705656096105No Hit
CCGTGTCTCAGTTCCAGTGTGGCTGATCATCCTCTCAGACCAGCTAGGGA1023460.4265007547920701No Hit
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC982750.40953590445342947No Hit
CGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCG969550.4040351423686824No Hit
GGCTGATCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCT955970.3983760250117985No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGATCATCCTCTCAGACCAGCT907790.3782982434024714No Hit
CGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATT905920.37751896877379887No Hit
GTTAGCTCAACGCCTCACAACGCTTACACACCCTGCCTATCAACGTTCTA897370.373955975150724No Hit
GGCAATTAGTACAGGTTAGCTCAACGCCTCACAACGCTTACACACCCTGC861570.35903724161784917No Hit
CAACGTTCTAGTCTCGAACAACCCTTTAGGACAGTTAAACTGTCAGGGAA836040.3483982676766677No Hit
ATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGA820840.3420640567912013No Hit
CCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTG769680.32074443646880246No Hit
CTCGAACAACCCTTTAGGACAGTTAAACTGTCAGGGAAGACTCATCTCAG764180.3184524522668245No Hit
CACGTGTGTAGCCCTACTCGTAAGGGCCATGATGACTTGACGTCGTCCCC751510.3131725541142679No Hit
CCTGCATGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCG746940.31126812360462436No Hit
GCTCGCTTCGCGCTTAGATGCTTTCAGCGCTTATCAATTCCGAACTTAGC716180.2984496810495621No Hit
GTACAGGTTAGCTCAACGCCTCACAACGCTTACACACCCTGCCTATCAAC701510.29233633409628623No Hit
CCTAGCGCTACCTTGTTCCGACTTCACCCCAGTCATGAACCACAAAGTGG687730.28659387185933055No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATATCA687600.28653969768728377No Hit
GTCGACGTATAAGCCGGGTTCTGTTCCGCTTGCGCGGTAGTAGCCATTCC686030.2858854403787191No Hit
CCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGG684670.28531869519423003No Hit
CTCTACAGTACTCTAGCTTGTCAGTTTCAAATGCGATTCCTAGGTTGAGC666590.27778431803572784No Hit
CAGGTTAGCTCAACGCCTCACAACGCTTACACACCCTGCCTATCAACGTT660170.275108947385419No Hit
CTCACGGGCAATTAGTACAGGTTAGCTCAACGCCTCACAACGCTTACACA655050.2729753184555777No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT651050.2713084208541392No Hit
TGGCAAACAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATT644660.2686455519358411No Hit
GGGTACGATTCCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCATG631470.26314895709509756No Hit
CTAGTCTCGAACAACCCTTTAGGACAGTTAAACTGTCAGGGAAGACTCAT625770.26077362801304765No Hit
GTACGATTCCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCATGGC609850.2541393755593223No Hit
CGATTCCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCATGGCATC594420.24770931806177313No Hit
GGTTAGCTCAACGCCTCACAACGCTTACACACCCTGCCTATCAACGTTCT585310.2439129587744969No Hit
CAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGC582060.24255860447332808No Hit
GTCTCGAACAACCCTTTAGGACAGTTAAACTGTCAGGGAAGACTCATCTC578650.24113757426810173No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGC563270.23472835299057057No Hit
CTGGCACGGAGTTAGCCGGTGCTTCTTCTGTAGGTAACGTCAAATGATTA558030.23254471713268607No Hit
CGTCGCCTTGGTGAGCCCTTACCTCACCAACTAGCTAATCCCACCTGGGC557580.23235719115252423No Hit
CCTAGCGCTACCTTGTTTCGACTTCACCCCAGTCATGAACCACAAAGTGG553400.230615283159021No Hit
GTCGCCTTGGTGAGCCCTTACCTCACCAACTAGCTAATCCCACCTGGGCA547690.22823578683296747No Hit
GTGGAGTCGACGTATAAGCCGGGTTCTGTTCCGCTTGCGCGGTAGTAGCC534940.22292255072838218No Hit
GGGCAATTAGTACAGGTTAGCTCAACGCCTCACAACGCTTACACACCCTG526280.21931371742126773No Hit
CTCGCTTCGCGCTTAGATGCTTTCAGCGCTTATCAATTCCGAACTTAGCT507730.21158347979459655No Hit
CGCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTG501800.2091123041004639No Hit
CACCTGCATGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTC497720.2074120685469966No Hit
CACGGAGTTAGCCGGTGCTTCTTCTGTAGGTAACGTCAAATGATTAAGGT496250.20679948367846793No Hit
ACAGGTTAGCTCAACGCCTCACAACGCTTACACACCCTGCCTATCAACGT496170.2067661457264392No Hit
CTTTAGGACAGTTAAACTGTCAGGGAAGACTCATCTCAGGGCTCGCTTCG494380.20602020904979543No Hit
CCTGGAGTTCCCACCATTACGTGCTGGCAAACAAGGATAAGGGTTGCGCT483090.2013153905697352No Hit
CTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCTTACCTCACCAA477030.1987900407035558No Hit
CCCAGGCGGTCTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGGC477020.19878587345955218No Hit
GTCAGTATCTGTCCAGGGGGCCGCCTTCGCCACCGGTATTCCTTCAGATC468890.1953979040846284No Hit
CTTGGTATTCTCTACCCGACCACCTGTGTCGGTTTGGGGTACGATTCCTG468600.1952770540085241No Hit
GTGTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTA462900.1929017249264742No Hit
GCAGGTTAGCTCAACGCCTCACAACGCTTACACACCCTGCCTATCAACGT461240.19220996242187718No Hit
CTGGAGTTCCCACCATTACGTGCTGGCAAACAAGGATAAGGGTTGCGCTC457620.19070142009257532No Hit
CCCACCTTCGTGCTCCTCCGTTACTCTTTGGGAGGAGACCGCCCCAGTCA442910.18457140416328513No Hit
CCCTGGAGTTCCCACCATTACGTGCTGGCAAACAAGGATAAGGGTTGCGC438590.1827711547537315No Hit
GCTGATCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCTT436270.18180435414489715No Hit
ATGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTA426610.1777787964374231No Hit
TGATCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCTTAC426280.1776412773853044No Hit
CTCAACGCCTCACAACGCTTACACACCCTGCCTATCAACGTTCTAGTCTC415380.1730989814213844No Hit
GGCTCGCTTCGCGCTTAGATGCTTTCAGCGCTTATCAATTCCGAACTTAG413690.17239471718477664No Hit
GTCTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGGCACAACCTC400530.16691062407604387No Hit
CCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTT398000.165856311343134No Hit
GGCAAACAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTT397690.16572712677902252No Hit
GTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGACG396770.16534374033069166No Hit
CCAGTGTGGCTGATCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGT394520.16440611042988248No Hit
GGAGTCGACGTATAAGCCGGGTTCTGTTCCGCTTGCGCGGTAGTAGCCAT390840.16287256463655903No Hit
TAGCTCAACGCCTCACAACGCTTACACACCCTGCCTATCAACGTTCTAGT388740.1619974433958038No Hit
CCCCAGGCGGTCTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGG388290.16180991741564196No Hit
CTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGGCACAACCTCCA383520.1598221420259265No Hit
CCCTAGCGCTACCTTGTTACGACTTCACCCCAGTCATGAACCACAAAGTG376740.1569967505914882No Hit
CTGCCTATCAACGTTCTAGTCTCGAACAACCCTTTAGGACAGTTAAACTG356430.14853307802018403No Hit
CCTTGGTATTCTCTACCCGACCACCTGTGTCGGTTTGGGGTACGATTCCT355510.1481496915718532No Hit
TACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTA355030.14794966385968056No Hit
GGGCTCGCTTCGCGCTTAGATGCTTTCAGCGCTTATCAATTCCGAACTTA351370.14642445255436431No Hit
GCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGTAGGTAACGTCAA345970.1441741407924223No Hit
CCCTGCTTTGCTCTTGCGAGGTTATGCGGTATTAGCCATCGTTTCCAATG345690.1440574579603216No Hit
GCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACC343080.14296980727538294No Hit
TACGATTCCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCATGGCA335560.1398360397846785No Hit
CAGGGCTCGCTTCGCGCTTAGATGCTTTCAGCGCTTATCAATTCCGAACT334330.13932346877223617No Hit
TCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGTA332420.13852752516754926No Hit
CTAGCGCTACCTTGTTACGACTTCACCCCAGTCATGAACCACAAAGTGGC330210.13760656424275447No Hit
GGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTT330100.1375607245587149No Hit
GGTGTGTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGA324630.1352812420887477No Hit
GCGCTACCTTGTTACGACTTCACCCCAGTCATGAACCACAAAGTGGCAAG319420.133110107962874No Hit
GGTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGAC316940.13207663144998213No Hit
ATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAG316820.13202662452193897No Hit
CTCCAATCCGGACTACGACGTACTTTGTGAGATTCGCTCCACCTCGCGGT316390.13184743302978433No Hit
CGACGTATAAGCCGGGTTCTGTTCCGCTTGCGCGGTAGTAGCCATTCCTC316280.13180159334574476No Hit
CGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGC314210.13093897383700034No Hit
CCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTG313470.13063059778073421No Hit
GCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGC312930.13040556660454No Hit
GTCTCAGTTCCAGTGTGGCTGATCATCCTCTCAGACCAGCTAGGGATCGT311190.12968046614791423No Hit
AGGCGGTCTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGGCACA305400.12726763186983195No Hit
GTTCTAGTCTCGAACAACCCTTTAGGACAGTTAAACTGTCAGGGAAGACT303170.12633833645703No Hit
GGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTG303040.12628416228498324No Hit
CTGATCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCTTA300560.12525068577209134No Hit
CTCGCAGTTAAGCGGGCTTATGCCATTGCACTAACCTCACGATGTCCAAC296160.12341709841050896No Hit
CTCCGTTACTCTTTGGGAGGAGACCGCCCCAGTCAAACTACCCACCAGGC292070.12171269561303806No Hit
GCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGC290430.12102926759644825No Hit
GCACGGAGTTAGCCGGTGCTTCTTCTGTAGGTAACGTCAAATGATTAAGG289210.12052086382800951No Hit
GGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTCC283750.11824554860204592No Hit
GCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT283720.11823304687003512No Hit
CGTCAATTCATTTGAGTTTTAATCTTGCGACCGTACTCCCCAGGCGGTCT282810.11785382766570786No Hit
CGGTGCTTCTTCTGTAGGTAACGTCAAATGATTAAGGTATTAACTTAACC278070.1158785540080032No Hit
CTCACCAACTAGCTAATCCCACCTGGGCATATCCGGTAGCGCAAGGCCCG274800.1145158652188272No Hit
CTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACC274760.1144991962428128No Hit
CGCTACCTTGTTACGACTTCACCCCAGTCATGAACCACAAAGTGGCAAGC272110.11339487658185979No Hit
TGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTAC271960.11333236792180584No Hit
AGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCA270470.11271144856526998No Hit
CCCGGTTTCGGGTCTATATCCAGAGACTGAGCGCCCAGTTAAGACTCGCT269980.11250725360909376No Hit
ACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAG268410.11185299630052914No Hit
CTCCCCAGGCGGTCTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAG268410.11185299630052914No Hit
CGGCAGTCTCCCTGGAGTTCCCACCATTACGTGCTGGCAAACAAGGATAA268110.11172797898042124No Hit
CGCGTTAGCTCCGAAAGCCACACCTCTAGGGCACAACCTCCAAGTAGACA268040.11169880827239606No Hit
CTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGGCACAACCTCCAAGT267940.1116571358323601No Hit
CCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATT265480.1106319938074754No Hit
TCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGT265410.11060282309945024No Hit
GCCCACCTTCGTGCTCCTCCGTTACTCTTTGGGAGGAGACCGCCCCAGTC264800.11034862121523087No Hit
GGCTAATCCAGGTTCGCTCGCCGCTACTACCGGAATCTCGGTTGATTTCT263930.10998607098691797No Hit
CCACCTGCATGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCT262960.10958184831856913No Hit
GTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGATCATCCTCTCAGACCAG262530.10940265682641448No Hit
ATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCTTACCTCAC262340.10932347919034617No Hit
CGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA261810.10910261525815557No Hit
CCCACCATTACGTGCTGGCAAACAAGGATAAGGGTTGCGCTCGTTGCGGG260620.1086067132217276No Hit
GGAAGACTCATCTCAGGGCTCGCTTCGCGCTTAGATGCTTTCAGCGCTTA254550.10607719611154462No Hit
CCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCATGGCATCAATGA253350.10557712683111305No Hit
CCTCTGTATACGCCATTGTAGCACGTGTGTAGCCCTACTCGTAAGGGCCA251150.10466033315032186No Hit
CCCATATTCAGACAGGATATCACGTGTCCCGCCCTACTCGATTTCACGCT250380.10433945536204495No Hit
ACCTGCATGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCC248600.10359768592940481No Hit
GTGTGTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGAT247890.10330181160514947No Hit
CACCGGATCACTATGACCTGCTTTCGCACCTGCTCGAACCGTCATTCTCG247500.10313928908900921No Hit
GTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCA244510.10189328313193391No Hit
CAGGCGGTCTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGGCAC243170.101334872435452No Hit
CCAGGCGGTCTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGGCA242880.1012140223593477No Hit
CCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCA242770.10116818267530814No Hit
CCTGCTTTCGCACCTGCTCGAACCGTCATTCTCGCAGTTAAGCGGGCTTA242640.1011140085032614No Hit
CTACAGTACTCTAGCTTGTCAGTTTCAAATGCGATTCCTAGGTTGAGCCC242220.10093898425511035No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)