FastQCFastQC Report
Wed 6 Sep 2023
6188_NO2_S8_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename6188_NO2_S8_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28263506
Sequences flagged as poor quality0
Sequence length200
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGTTCCAGTGTGGCTGATCATCCTCTCAGACCAGCTAGGGATCGTCG3952021.398276632771603No Hit
CGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCG2649320.9373642463182027No Hit
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC2585170.9146671329452192No Hit
GTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACT2448520.8663185664227219No Hit
CTGGCAAACAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACAT2293930.8116225920450209No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGT2245070.7943352816879831No Hit
CCCCTCTACAGTACTCTAGCTTGTCAGTTTCAAATGCGATTCCTAGGTTG2222610.7863886384088371No Hit
CACGTGTGTAGCCCTACTCGTAAGGGCCATGATGACTTGACGTCGTCCCC1711500.6055512009019688No Hit
CCCCCCTCTACAGTACTCTAGCTTGTCAGTTTCAAATGCGATTCCTAGGT1701600.6020484507477594No Hit
CGGGCAATTAGTACAGGTTAGCTCAACGCCTCACAACGCTTACACACCCT1468370.5195286104986409No Hit
CGTGTCTCAGTTCCAGTGTGGCTGATCATCCTCTCAGACCAGCTAGGGAT1436520.5082596617701993No Hit
TTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAG1392350.49263173507207486No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATATCA1364480.482770962668255No Hit
CCTAGCGCTACCTTGTTACGACTTCACCCCAGTCATGAACCACAAAGTGG1255460.44419825339432406No Hit
CCGTGTCTCAGTTCCAGTGTGGCTGATCATCCTCTCAGACCAGCTAGGGA1109560.3925769152630958No Hit
ATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGA1082690.38306995600616567No Hit
CCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTG1046940.37042113600485377No Hit
CGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATT965970.34177288550118307No Hit
CAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGC953850.33748467016087813No Hit
CTCTACAGTACTCTAGCTTGTCAGTTTCAAATGCGATTCCTAGGTTGAGC907520.32109250706547166No Hit
GGCTGATCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCT890060.314914929520775No Hit
GTACGATTCCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCATGGC882980.31240993244079485No Hit
CGATTCCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCATGGCATC879150.3110548280882067No Hit
CCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGG843960.29860414344915315No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGATCATCCTCTCAGACCAGCT834920.29540567260126893No Hit
GGGTACGATTCCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCATG833430.29487849101240304No Hit
TGGCAAACAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATT817700.2893130102118258No Hit
CCCACCTTCGTGCTCCTCCGTTACTCTTTGGGAGGAGACCGCCCCAGTCA807130.28557320524920016No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT790140.2795619198835417No Hit
CAACGTTCTAGTCTCGAACAACCCTTTAGGACAGTTAAACTGTCAGGGAA767780.27165065791908477No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGC709700.2511011903477226No Hit
CCTGCATGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCG697170.24666791161719287No Hit
GTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGACG690120.24417352893161948No Hit
GCTCGCTTCGCGCTTAGATGCTTTCAGCGCTTATCAATTCCGAACTTAGC689350.24390109280851427No Hit
CCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCA683440.2418100571103953No Hit
CTGGCACGGAGTTAGCCGGTGCTTCTTCTGTAGGTAACGTCAAATGATTA678450.24004452950741498No Hit
GGCAATTAGTACAGGTTAGCTCAACGCCTCACAACGCTTACACACCCTGC641390.22693221428367735No Hit
GTTAGCTCAACGCCTCACAACGCTTACACACCCTGCCTATCAACGTTCTA632630.2238328111169223No Hit
CTCGAACAACCCTTTAGGACAGTTAAACTGTCAGGGAAGACTCATCTCAG618480.218826355088431No Hit
CCTAGCGCTACCTTGTTCCGACTTCACCCCAGTCATGAACCACAAAGTGG616820.21823902526459388No Hit
CTTGGTATTCTCTACCCGACCACCTGTGTCGGTTTGGGGTACGATTCCTG614170.21730142042533576No Hit
CTCCGTTACTCTTTGGGAGGAGACCGCCCCAGTCAAACTACCCACCAGGC603810.21363591622355696No Hit
CACGGAGTTAGCCGGTGCTTCTTCTGTAGGTAACGTCAAATGATTAAGGT598090.21161210502334704No Hit
GTGTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTA590330.20886651500348188No Hit
CCTGCTTTCGCACCTGCTCGAACCGTCATTCTCGCAGTTAAGCGGGCTTA584110.20666579722982706No Hit
CGCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTG565380.20003887698858028No Hit
GTACAGGTTAGCTCAACGCCTCACAACGCTTACACACCCTGCCTATCAAC546780.19345795245642916No Hit
ATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAG546700.19342964740467794No Hit
CTAGTCTCGAACAACCCTTTAGGACAGTTAAACTGTCAGGGAAGACTCAT538500.19052837960018124No Hit
CCTCTGTATACGCCATTGTAGCACGTGTGTAGCCCTACTCGTAAGGGCCA534530.18912374140702853No Hit
GGTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGAC532500.18840550071884216No Hit
CTCGCTTCGCGCTTAGATGCTTTCAGCGCTTATCAATTCCGAACTTAGCT530430.1876731075047802No Hit
CTGGAGTTCCCACCATTACGTGCTGGCAAACAAGGATAAGGGTTGCGCTC528520.1869973243942206No Hit
GGCAAACAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTT523120.18508673340101545No Hit
CCTGGAGTTCCCACCATTACGTGCTGGCAAACAAGGATAAGGGTTGCGCT518900.18359364192114028No Hit
GTCAGTATCTGTCCAGGGGGCCGCCTTCGCCACCGGTATTCCTTCAGATC516610.18278340981476254No Hit
CCCGGTTTCGGGTCTATATCCAGAGACTGAGCGCCCAGTTAAGACTCGCT512790.18143184359364334No Hit
CCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCAC511780.1810744923152846No Hit
CCCAGGCGGTCTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGGC511350.18092235266212195No Hit
CCTAGCGCTACCTTGTTTCGACTTCACCCCAGTCATGAACCACAAAGTGG509720.1803456372326915No Hit
CTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCTTACCTCACCAA503630.17819091516813237No Hit
TACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTA503250.17805646617231422No Hit
GTCGACGTATAAGCCGGGTTCTGTTCCGCTTGCGCGGTAGTAGCCATTCC495850.17543824888532936No Hit
CGTCGCCTTGGTGAGCCCTTACCTCACCAACTAGCTAATCCCACCTGGGC485240.17168429139682811No Hit
CAGGTTAGCTCAACGCCTCACAACGCTTACACACCCTGCCTATCAACGTT483200.17096251257717282No Hit
CCTTGGTATTCTCTACCCGACCACCTGTGTCGGTTTGGGGTACGATTCCT480800.17011336102463723No Hit
CAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAT477480.16893870137696293No Hit
CACCTGCATGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTC471960.16698565280613098No Hit
CTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTA471620.16686535633618846No Hit
CCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTT469920.1662638739864757No Hit
CTCCAATCCGGACTACGACGTACTTTGTGAGATTCGCTCCACCTCGCGGT467900.16554917142975822No Hit
CCCTGGAGTTCCCACCATTACGTGCTGGCAAACAAGGATAAGGGTTGCGC466640.16510336686467703No Hit
TGTAGCCCTACTCGTAAGGGCCATGATGACTTGACGTCGTCCCCACCTTC460570.16295572106305567No Hit
GTCTCGAACAACCCTTTAGGACAGTTAAACTGTCAGGGAAGACTCATCTC458770.16231885739865393No Hit
GTCGCCTTGGTGAGCCCTTACCTCACCAACTAGCTAATCCCACCTGGGCA455900.1613034136670801No Hit
GCACGTGTGTAGCCCTACTCGTAAGGGCCATGATGACTTGACGTCGTCCC455490.16115835027685524No Hit
TACGATTCCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCATGGCA453370.16040826640544878No Hit
CGTGTGTAGCCCTACTCGTAAGGGCCATGATGACTTGACGTCGTCCCCAC447660.15838799333670778No Hit
CTAAGTTAGAACATCAAACATACAAGGGTGGTATTTCAAGGACGGCTCCA447280.15825354434088965No Hit
CGTCAATTCATTTGAGTTTTAATCTTGCGACCGTACTCCCCAGGCGGTCT446510.15798110821778444No Hit
TGATCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCTTAC444340.1572133336890335No Hit
CTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGACGACAGCC444330.1572097955575646No Hit
CCCCAGTCAAACTACCCACCAGGCACTGTCCTCAACCCAGATAATGGGTC426830.15101806548699231No Hit
CCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTC417030.14755069664747184No Hit
CTCGGTTGATTTCTTCTCCTCGGGGTACTTAGATGTTTCAGTTCCCCCGG415560.14703059132154375No Hit
GCCCACCTTCGTGCTCCTCCGTTACTCTTTGGGAGGAGACCGCCCCAGTC411350.1455410379731375No Hit
GCTGATCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCTT407990.14435222579958765No Hit
GGCTCGCTTCGCGCTTAGATGCTTTCAGCGCTTATCAATTCCGAACTTAG405980.14364106137433905No Hit
GTGTAGCCCTACTCGTAAGGGCCATGATGACTTGACGTCGTCCCCACCTT398470.14098392464119633No Hit
GGCTAATCCAGGTTCGCTCGCCGCTACTACCGGAATCTCGGTTGATTTCT396000.1401100061683784No Hit
CCCTGCTTTGCTCTTGCGAGGTTATGCGGTATTAGCCATCGTTTCCAATG395480.1399260233319957No Hit
GCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGTAGGTAACGTCAA395060.13977742181030195No Hit
CTCGCAGTTAAGCGGGCTTATGCCATTGCACTAACCTCACGATGTCCAAC391520.1385249232703119No Hit
CCCATATTCAGACAGGATATCACGTGTCCCGCCCTACTCGATTTCACGCT390480.13815695759754645No Hit
CACCGGATCACTATGACCTGCTTTCGCACCTGCTCGAACCGTCATTCTCG390150.13804019925907282No Hit
CGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGC388090.13731134417647972No Hit
ACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAG388050.13729719165060414No Hit
CGCCATTGTAGCACGTGTGTAGCCCTACTCGTAAGGGCCATGATGACTTG386830.13686553961139852No Hit
GGTTAGCTCAACGCCTCACAACGCTTACACACCCTGCCTATCAACGTTCT384640.13609068881970976No Hit
GGTGTGTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGA383930.13583948148541797No Hit
CCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTG382010.13516016024338948No Hit
CTTCAATCTTCCAACGCCCACGGCAGATAGGGACCGAACTGTCTCACGAC381820.1350929357454804No Hit
GCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGACGACA380330.13456575415661454No Hit
CCCCAGGCGGTCTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGG376550.13322834046137094No Hit
CTGCTTTCGCACCTGCTCGAACCGTCATTCTCGCAGTTAAGCGGGCTTAT376390.13317173035786856No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATATCACGTGT374100.1323614982514908No Hit
GTCTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGGCACAACCTC372450.13177770655912255No Hit
CTTTAGGACAGTTAAACTGTCAGGGAAGACTCATCTCAGGGCTCGCTTCG368360.1303306107883431No Hit
CTCACGGGCAATTAGTACAGGTTAGCTCAACGCCTCACAACGCTTACACA367220.12992726380088868No Hit
CTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGGCACAACCTCCA366670.12973266657009927No Hit
GTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACA364900.12910641730010425No Hit
CTGGGTTGTTTCCCTCTCCACGACGGACGTTAGCACCCGCCGTGTGTCTC362900.12839879100632456No Hit
CTCTGTATACGCCATTGTAGCACGTGTGTAGCCCTACTCGTAAGGGCCAT359980.1273656566174062No Hit
ATGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTA359650.12724889827893257No Hit
CCGACTTCATGGAGTCGAGTTGCAGACTCCAATCCGGACTACGACGTACT357070.12633606035995676No Hit
GGGCAATTAGTACAGGTTAGCTCAACGCCTCACAACGCTTACACACCCTG353760.12516493884375138No Hit
CGGTGCTTCTTCTGTAGGTAACGTCAAATGATTAAGGTATTAACTTAACC351880.12449977012759847No Hit
CTCAACGCCTCACAACGCTTACACACCCTGCCTATCAACGTTCTAGTCTC346040.12243350134976178No Hit
CTGATCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCTTA345990.12241581069241728No Hit
GGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTG345340.12218583214693889No Hit
CCACCTTCGTGCTCCTCCGTTACTCTTTGGGAGGAGACCGCCCCAGTCAA341650.12088026163491535No Hit
CAGGGCTCGCTTCGCGCTTAGATGCTTTCAGCGCTTATCAATTCCGAACT335620.1187467683591696No Hit
GGGCTCGCTTCGCGCTTAGATGCTTTCAGCGCTTATCAATTCCGAACTTA328420.11619931370156272No Hit
CTAGCGCTACCTTGTTACGACTTCACCCCAGTCATGAACCACAAAGTGGC327630.11591980131551974No Hit
ACAGGTTAGCTCAACGCCTCACAACGCTTACACACCCTGCCTATCAACGT327510.11587734373789296No Hit
GGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTCC327500.11587380560642406No Hit
GGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTT323400.11442317170417571No Hit
CCGGTTTCGGGTCTATATCCAGAGACTGAGCGCCCAGTTAAGACTCGCTT322100.1139632146132189No Hit
GCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGC321090.11360586333486015No Hit
CCAGTGTGGCTGATCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGT320730.1134784906019798No Hit
CCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCATGGCATCAATGA320640.11344664741875972No Hit
CGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA316210.11187925517803772No Hit
AGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCA315210.11152544203114788No Hit
GCACGGAGTTAGCCGGTGCTTCTTCTGTAGGTAACGTCAAATGATTAAGG313900.11106194680872217No Hit
CCCCAGTCAAACTACCCACCAGGCACTGTCCTCAACCCGGATAACGGGTC312400.11053122708838742No Hit
CTACAGTACTCTAGCTTGTCAGTTTCAAATGCGATTCCTAGGTTGAGCCC312390.11052768895691852No Hit
CCCTAGCGCTACCTTGTTACGACTTCACCCCAGTCATGAACCACAAAGTG308510.10915489394698591No Hit
AGGCGGTCTACTTAACGCGTTAGCTCCGAAAGCCACACCTCTAGGGCACA305980.10825974668535461No Hit
GTGTGTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGAT305420.1080616113230963No Hit
CGGCAGTCTCCCTGGAGTTCCCACCATTACGTGCTGGCAAACAAGGATAA304890.10787409035524467No Hit
CTGGTATCTGCGACTCTCGTCAGCTCCATCCGCGAGGGACTTCACCATCA303880.10751673907688593No Hit
GCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGC302470.10701786253977125No Hit
CGCGTTAGCTCCGAAAGCCACACCTCTAGGGCACAACCTCCAAGTAGACA298960.1057759783941879No Hit
TCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGTA296570.10493036497312117No Hit
GTCGGTTTGGGGTACGATTCCTGACTATCTGAAGCTTAGAGGCTTTTCCT293940.10399983639680088No Hit
GTCTCAGTTCCAGTGTGGCTGATCATCCTCTCAGACCAGCTAGGGATCGT293500.10384415861216935No Hit
CTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCCGA292410.10345850228205941No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT290370.10273672346240413No Hit
GTTGATTTCTTCTCCTCGGGGTACTTAGATGTTTCAGTTCCCCCGGTTTG288770.10217062242738038No Hit
GTGGAGTCGACGTATAAGCCGGGTTCTGTTCCGCTTGCGCGGTAGTAGCC288100.10193356761896419No Hit
AGCGATTCCGACTTCATGGAGTCGAGTTGCAGACTCCAATCCGGACTACG286400.10133208526925146No Hit
CTCACCAACTAGCTAATCCCACCTGGGCATATCCGGTAGCGCAAGGCCCG285910.10115871682727542No Hit
CTGCCTATCAACGTTCTAGTCTCGAACAACCCTTTAGGACAGTTAAACTG285120.10087920444123244No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)