FastQCFastQC Report
Wed 6 Sep 2023
6192_GSH3_S12_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename6192_GSH3_S12_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26830657
Sequences flagged as poor quality0
Sequence length200
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCG6346632.3654396536022206No Hit
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC5818692.1686722021007534No Hit
CTCAGTTCCAGTGTGGCTGATCATCCTCTCAGACCAGCTAGGGATCGTCG3067711.1433600004651396No Hit
CACGTGTGTAGCCCTACTCGTAAGGGCCATGATGACTTGACGTCGTCCCC2783691.0375034796948879No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATATCA2333570.8697401632766577No Hit
CTGGCAAACAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACAT2053850.7654862868248064No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGT1848060.6887867114100112No Hit
CCCCTCTACAGTACTCTAGCTTGTCAGTTTCAAATGCGATTCCTAGGTTG1373330.5118510515787966No Hit
CCCACCTTCGTGCTCCTCCGTTACTCTTTGGGAGGAGACCGCCCCAGTCA1273360.4745914347158924No Hit
CCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCA1263080.47075999667097235No Hit
CGTGTCTCAGTTCCAGTGTGGCTGATCATCCTCTCAGACCAGCTAGGGAT1163010.4334631090099657No Hit
TTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAG1082610.4034973873356884No Hit
CCCCCCTCTACAGTACTCTAGCTTGTCAGTTTCAAATGCGATTCCTAGGT1062410.39596868611901676No Hit
CGATTCCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCATGGCATC1051130.3917645400930734No Hit
CCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCAC969800.36145219999644435No Hit
GTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACT958970.3574157725619615No Hit
GGCTAATCCAGGTTCGCTCGCCGCTACTACCGGAATCTCGGTTGATTTCT949850.35401667577502854No Hit
GGGTACGATTCCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCATG936880.34918265326115566No Hit
CCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTC921050.3432826859215561No Hit
GTACGATTCCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCATGGC914620.3408861736035759No Hit
TGTAGCCCTACTCGTAAGGGCCATGATGACTTGACGTCGTCCCCACCTTC910060.33918662521010945No Hit
CCGTGTCTCAGTTCCAGTGTGGCTGATCATCCTCTCAGACCAGCTAGGGA870650.32449820367797927No Hit
CCTGCTTTCGCACCTGCTCGAACCGTCATTCTCGCAGTTAAGCGGGCTTA852560.31775591630126687No Hit
CTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTA809070.3015468462065614No Hit
CAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAT799810.2980955703022852No Hit
CCTCTGTATACGCCATTGTAGCACGTGTGTAGCCCTACTCGTAAGGGCCA796630.2969103589226309No Hit
CGTGTGTAGCCCTACTCGTAAGGGCCATGATGACTTGACGTCGTCCCCAC793230.29564315178715156No Hit
TGGCAAACAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATT772610.2879579132184501No Hit
ATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGA769180.28667952484354No Hit
CTTGGTATTCTCTACCCGACCACCTGTGTCGGTTTGGGGTACGATTCCTG760980.28362331939914853No Hit
GTGTAGCCCTACTCGTAAGGGCCATGATGACTTGACGTCGTCCCCACCTT734650.2738099182588037No Hit
GCACGTGTGTAGCCCTACTCGTAAGGGCCATGATGACTTGACGTCGTCCC718080.26763414701324684No Hit
CTCCGTTACTCTTTGGGAGGAGACCGCCCCAGTCAAACTACCCACCAGGC717730.26750369921988865No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGATCATCCTCTCAGACCAGCT705620.2629902055696959No Hit
CCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTG705100.26279639741956373No Hit
GGCTGATCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCT698250.26024334774955377No Hit
CTGGAGTTCCCACCATTACGTGCTGGCAAACAAGGATAAGGGTTGCGCTC685860.2556254958646745No Hit
CCTGCATGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCG681130.2538625871144341No Hit
GTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACA671540.2502883175764201No Hit
GCCCACCTTCGTGCTCCTCCGTTACTCTTTGGGAGGAGACCGCCCCAGTC667510.24878630441289604No Hit
CCTGGAGTTCCCACCATTACGTGCTGGCAAACAAGGATAAGGGTTGCGCT663630.2473401974465254No Hit
CTCCAATCCGGACTACGACGTACTTTGTGAGATTCGCTCCACCTCGCGGT663380.2472470204512696No Hit
CCCCAGTCAAACTACCCACCAGGCACTGTCCTCAACCCAGATAATGGGTC659850.24593136127825718No Hit
CTCGCAGTTAAGCGGGCTTATGCCATTGCACTAACCTCACGATGTCCAAC658250.24533502850861982No Hit
CACCGGATCACTATGACCTGCTTTCGCACCTGCTCGAACCGTCATTCTCG650370.24239808961815582No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATATCACGTGT643560.23985994826738682No Hit
CGCCATTGTAGCACGTGTGTAGCCCTACTCGTAAGGGCCATGATGACTTG640190.23860392237133812No Hit
CTCGGTTGATTTCTTCTCCTCGGGGTACTTAGATGTTTCAGTTCCCCCGG635510.23685964902014886No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT615510.22940548939968186No Hit
CCCATATTCAGACAGGATATCACGTGTCCCGCCCTACTCGATTTCACGCT598430.22303963708380303No Hit
CCCGGTTTCGGGTCTATATCCAGAGACTGAGCGCCCAGTTAAGACTCGCT595770.22204823385428094No Hit
CCCTGGAGTTCCCACCATTACGTGCTGGCAAACAAGGATAAGGGTTGCGC593180.22108292018343045No Hit
CCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGG589810.21982689428738178No Hit
CCTTGGTATTCTCTACCCGACCACCTGTGTCGGTTTGGGGTACGATTCCT583480.217467652767504No Hit
CGTCGCCTTGGTGAGCCCTTACCTCACCAACTAGCTAATCCCACCTGGGC576180.21474688450603352No Hit
CTAAGTTAGAACATCAAACATACAAGGGTGGTATTTCAAGGACGGCTCCA571110.21285725504224515No Hit
GTCGCCTTGGTGAGCCCTTACCTCACCAACTAGCTAATCCCACCTGGGCA566930.21129933568156756No Hit
CTGGCACGGAGTTAGCCGGTGCTTCTTCTGTAGGTAACGTCAAATGATTA564060.2102296637760305No Hit
CTGCTTTCGCACCTGCTCGAACCGTCATTCTCGCAGTTAAGCGGGCTTAT556280.20732999568366886No Hit
GCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATT549490.20479930849252032No Hit
CGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATT541720.20190336747996893No Hit
CTCTACAGTACTCTAGCTTGTCAGTTTCAAATGCGATTCCTAGGTTGAGC538720.20078524353689883No Hit
CCACCTTCGTGCTCCTCCGTTACTCTTTGGGAGGAGACCGCCCCAGTCAA536940.20012182333067732No Hit
TACGATTCCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCATGGCA528990.19715879488154167No Hit
CCGACTTCATGGAGTCGAGTTGCAGACTCCAATCCGGACTACGACGTACT526380.19618602705107074No Hit
CACGGAGTTAGCCGGTGCTTCTTCTGTAGGTAACGTCAAATGATTAAGGT522750.19483309707995594No Hit
GGCAAACAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTT510840.19039414502596785No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT503850.18778891623861466No Hit
CGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGA503500.1876584684452565No Hit
GGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCGAC498660.18585456181710347No Hit
GTCAGTATCTGTCCAGGGGGCCGCCTTCGCCACCGGTATTCCTTCAGATC488440.18204548625104486No Hit
CTCTGTATACGCCATTGTAGCACGTGTGTAGCCCTACTCGTAAGGGCCAT487680.18176222818546708No Hit
GCCATGATGACTTGACGTCGTCCCCACCTTCCTCCGGTTTATCACCGGCA485720.18103172054266134No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGC478380.17829604396194995No Hit
GTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGACG475470.177211463737172No Hit
CGGATCACTATGACCTGCTTTCGCACCTGCTCGAACCGTCATTCTCGCAG472900.17625360422594197No Hit
CCCCAGTCAAACTACCCACCAGGCACTGTCCTCAACCCGGATAACGGGTC471260.1756423631370637No Hit
GGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCGA468680.17468077654602343No Hit
GTTGATTTCTTCTCCTCGGGGTACTTAGATGTTTCAGTTCCCCCGGTTTG467400.17420371033031357No Hit
CCGGATCACTATGACCTGCTTTCGCACCTGCTCGAACCGTCATTCTCGCA457180.17039463476425493No Hit
CTGGTATCTGCGACTCTCGTCAGCTCCATCCGCGAGGGACTTCACCATCA448120.1670179004561834No Hit
CCCCACCTTCCTCCGGTTTATCACCGGCAGTCTCCCTGGAGTTCCCACCA441840.16467729433535674No Hit
CGCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTG428160.1595786491549573No Hit
CTGGGTTGTTTCCCTCTCCACGACGGACGTTAGCACCCGCCGTGTGTCTC426120.1588183248736697No Hit
CAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGC421820.1572156805552693No Hit
ATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAG418240.1558813859832057No Hit
CGGACTACGACGTACTTTGTGAGATTCGCTCCACCTCGCGGTATCGCTGC417230.1555049509223721No Hit
AGCACGTGTGTAGCCCTACTCGTAAGGGCCATGATGACTTGACGTCGTCC413880.1542563791859439No Hit
CCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACC407520.15188595642663538No Hit
CCGGACTACGACGTACTTTGTGAGATTCGCTCCACCTCGCGGTATCGCTG406800.15161760668029858No Hit
CACCTGCATGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTC406460.15149088596675064No Hit
CTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCTTACCTCACCAA404100.15061129513153554No Hit
AGCGATTCCGACTTCATGGAGTCGAGTTGCAGACTCCAATCCGGACTACG401390.14960125650296227No Hit
CCGGTTTCGGGTCTATATCCAGAGACTGAGCGCCCAGTTAAGACTCGCTT399700.1489713800150328No Hit
CCCACCATTACGTGCTGGCAAACAAGGATAAGGGTTGCGCTCGTTGCGGG398610.14856512831571733No Hit
GGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGAT390500.14554246658961797No Hit
GGTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGAC385920.14383546403653105No Hit
CTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCCGA382920.14271734009346101No Hit
GTAGCCCTACTCGTAAGGGCCATGATGACTTGACGTCGTCCCCACCTTCC378490.14106624373752755No Hit
GCCCTCTGTATACGCCATTGTAGCACGTGTGTAGCCCTACTCGTAAGGGC376230.14022392370041478No Hit
CCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTT373700.13928097250842572No Hit
GTCGGTTTGGGGTACGATTCCTGACTATCTGAAGCTTAGAGGCTTTTCCT372690.13890453744759215No Hit
GGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGAC366240.13650057096999152No Hit
ACCTGCTTTCGCACCTGCTCGAACCGTCATTCTCGCAGTTAAGCGGGCTT364890.13599741519561No Hit
CCGACATCGAGGTGCCAAACACCGCCGTCGATATGAACTCTTGGGCGGTA363850.13560979889534572No Hit
TGATCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCTTAC363620.13552407605971034No Hit
CTCTACCCGACCACCTGTGTCGGTTTGGGGTACGATTCCTGACTATCTGA360350.134305320961764No Hit
GACCTGCTTTCGCACCTGCTCGAACCGTCATTCTCGCAGTTAAGCGGGCT358560.1336381736757322No Hit
CGGCAGTCTCCCTGGAGTTCCCACCATTACGTGCTGGCAAACAAGGATAA355000.1323113332632891No Hit
ATGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTA352490.13137583623092047No Hit
CTTCTCCGTCCCCCCATCGCAATAGTCAGAAGTACGGGAATATTAACCCG345120.1286289784107784No Hit
CCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCATGGCATCAATGA344380.12835317450482112No Hit
CTCGATTTCACGCTCGATGAAGCGTCGGTTACGGGGCTATCACCCTGTAT339190.1264188200833099No Hit
GCTGATCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCTT337110.12564358748278137No Hit
CGCCTTGGTATTCTCTACCCGACCACCTGTGTCGGTTTGGGGTACGATTC336880.125557864647146No Hit
CGTCATTCTCGCAGTTAAGCGGGCTTATGCCATTGCACTAACCTCACGAT334110.1245254635397113No Hit
GCAGACTCCAATCCGGACTACGACGTACTTTGTGAGATTCGCTCCACCTC333800.12440992406559408No Hit
CCTGGAAGCATGGCATCAATGACTTCACTACCGTAGTAGCTCGACGTCGT331960.1237241413805111No Hit
ATGGAGTCGAGTTGCAGACTCCAATCCGGACTACGACGTACTTTGTGAGA331800.12366450810354737No Hit
CCAGTGTGGCTGATCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGT330070.12301972329637698No Hit
CCTACTCGTAAGGGCCATGATGACTTGACGTCGTCCCCACCTTCCTCCGG325270.12123072498746489No Hit
CTTAGCTGGCGGTCTGGGTTGTTTCCCTCTCCACGACGGACGTTAGCACC324650.12099964603923043No Hit
GTCGACGTATAAGCCGGGTTCTGTTCCGCTTGCGCGGTAGTAGCCATTCC317080.11817824662288368No Hit
TGGGGTACGATTCCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCA315680.11765645544945097No Hit
CCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTG311070.11593827165693334No Hit
GTCGGTTCGGTCCTCCAGTTGATGTTACTCAACCTTCAACCTGCCCATGG306110.11408964007105751No Hit
CGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGA305280.11378029244680814No Hit
TCCGACTTCATGGAGTCGAGTTGCAGACTCCAATCCGGACTACGACGTAC303490.11311314516077633No Hit
CTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCC302860.11287833913273164No Hit
TGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTATGACCTGCTTT301380.11232673132081708No Hit
CTGGAAGCATGGCATCAATGACTTCACTACCGTAGTAGCTCGACGTCGTG295280.11005321263657464No Hit
CGGGGTACGATTCCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCA288490.1075225254454261No Hit
CCCTACTCGTAAGGGCCATGATGACTTGACGTCGTCCCCACCTTCCTCCG287600.10719081534231532No Hit
CTGCCCTCTGTATACGCCATTGTAGCACGTGTGTAGCCCTACTCGTAAGG286390.10673983868527707No Hit
CGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGC286370.10673238452565659No Hit
CTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGACGACAGCC283850.10579316041347776No Hit
CCCCATCGCAATAGTCAGAAGTACGGGAATATTAACCCGTTTCCCATCGA283740.10575216253556519No Hit
CCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCCGACCACCTGTGTCG283050.10549499402865908No Hit
TCTGTATACGCCATTGTAGCACGTGTGTAGCCCTACTCGTAAGGGCCATG281760.10501420073313897No Hit
CTAGCCTTCTCCGTCCCCCCATCGCAATAGTCAGAAGTACGGGAATATTA280290.10446632000103463No Hit
GCACGGAGTTAGCCGGTGCTTCTTCTGTAGGTAACGTCAAATGATTAAGG279910.10432469096824577No Hit
GCGATTACTAGCGATTCCGACTTCATGGAGTCGAGTTGCAGACTCCAATC277730.10351218756961486No Hit
TCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGTA271500.10119021684783938No Hit
CGGGCAATTAGTACAGGTTAGCTCAACGCCTCACAACGCTTACACACCCT269280.10036280512996755No Hit
GCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGACGACA269190.10032926141167546No Hit
CTCACCAACTAGCTAATCCCACCTGGGCATATCCGGTAGCGCAAGGCCCG268350.10001618670761586No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)