FastQCFastQC Report
Wed 6 Sep 2023
6191_GSH2_S11_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename6191_GSH2_S11_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29922520
Sequences flagged as poor quality0
Sequence length200
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCG7729862.5832917815745464No Hit
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC6530652.182520055129047No Hit
CACGTGTGTAGCCCTACTCGTAAGGGCCATGATGACTTGACGTCGTCCCC3539621.1829284431926188No Hit
CTCAGTTCCAGTGTGGCTGATCATCCTCTCAGACCAGCTAGGGATCGTCG3410161.1396633705984656No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATATCA3096201.0347390527268425No Hit
CTGGCAAACAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACAT2331590.7792091040460496No Hit
CCCCTCTACAGTACTCTAGCTTGTCAGTTTCAAATGCGATTCCTAGGTTG2164480.7233615350578761No Hit
CCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCA1643690.5493153651497268No Hit
CCCCCCTCTACAGTACTCTAGCTTGTCAGTTTCAAATGCGATTCCTAGGT1571510.5251930652899555No Hit
CCCACCTTCGTGCTCCTCCGTTACTCTTTGGGAGGAGACCGCCCCAGTCA1568710.5242573152261241No Hit
GTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACT1541090.5150268092393288No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGT1485980.4966092428044162No Hit
CTCGGTTGATTTCTTCTCCTCGGGGTACTTAGATGTTTCAGTTCCCCCGG1426460.476717870018969No Hit
TTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAG1376460.460008047450549No Hit
CCTGCTTTCGCACCTGCTCGAACCGTCATTCTCGCAGTTAAGCGGGCTTA1351700.4517333433146673No Hit
CGATTCCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCATGGCATC1315240.4395485406977755No Hit
CAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAT1225180.40945080828753727No Hit
CCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCAC1211450.4048622910102492No Hit
GTACGATTCCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCATGGC1164090.3890347470734417No Hit
CTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTA1137830.3802587482605075No Hit
CGTGTCTCAGTTCCAGTGTGGCTGATCATCCTCTCAGACCAGCTAGGGAT1136480.37980758305116014No Hit
ATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGA1079320.36070491389094234No Hit
CTCCGTTACTCTTTGGGAGGAGACCGCCCCAGTCAAACTACCCACCAGGC1078120.36030387814930026No Hit
GGGTACGATTCCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCATG1056770.3531687839125849No Hit
CTGGGTTGTTTCCCTCTCCACGACGGACGTTAGCACCCGCCGTGTGTCTC1041760.3481524951775452No Hit
CCTCTGTATACGCCATTGTAGCACGTGTGTAGCCCTACTCGTAAGGGCCA1028260.3436408430840718No Hit
CCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTG1003930.33550984342227863No Hit
GTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACA974650.32572457132621185No Hit
CCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTC951910.3181249440220944No Hit
CTCTACAGTACTCTAGCTTGTCAGTTTCAAATGCGATTCCTAGGTTGAGC917220.3065316691241246No Hit
TGTAGCCCTACTCGTAAGGGCCATGATGACTTGACGTCGTCCCCACCTTC912680.30501441723491207No Hit
CTGCTTTCGCACCTGCTCGAACCGTCATTCTCGCAGTTAAGCGGGCTTAT908430.30359408231659635No Hit
CCCCAGTCAAACTACCCACCAGGCACTGTCCTCAACCCAGATAATGGGTC887190.2964957496895315No Hit
CGTGTGTAGCCCTACTCGTAAGGGCCATGATGACTTGACGTCGTCCCCAC870010.29075425465502236No Hit
CCGTGTCTCAGTTCCAGTGTGGCTGATCATCCTCTCAGACCAGCTAGGGA869810.2906874153647487No Hit
CCCGGTTTCGGGTCTATATCCAGAGACTGAGCGCCCAGTTAAGACTCGCT864390.288876070598332No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATATCACGTGT856990.2864030168582058No Hit
CTTGGTATTCTCTACCCGACCACCTGTGTCGGTTTGGGGTACGATTCCTG853350.2851865417752248No Hit
GTTGATTTCTTCTCCTCGGGGTACTTAGATGTTTCAGTTCCCCCGGTTTG851860.2846885890626859No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT850550.2842507917113933No Hit
CGCCATTGTAGCACGTGTGTAGCCCTACTCGTAAGGGCCATGATGACTTG828080.27674139744914533No Hit
GGCTAATCCAGGTTCGCTCGCCGCTACTACCGGAATCTCGGTTGATTTCT827450.27653085368478325No Hit
GCACGTGTGTAGCCCTACTCGTAAGGGCCATGATGACTTGACGTCGTCCC795150.26573630830558387No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT783740.2619231267954705No Hit
TGGCAAACAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATT756640.25286640296338675No Hit
GTGTAGCCCTACTCGTAAGGGCCATGATGACTTGACGTCGTCCCCACCTT756280.2527460922408941No Hit
CTAAGTTAGAACATCAAACATACAAGGGTGGTATTTCAAGGACGGCTCCA754860.252271533279951No Hit
CCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGG752290.2514126483999342No Hit
CTCTGTATACGCCATTGTAGCACGTGTGTAGCCCTACTCGTAAGGGCCAT733130.24500944439171568No Hit
CAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGC704990.2356051562502089No Hit
CACCGGATCACTATGACCTGCTTTCGCACCTGCTCGAACCGTCATTCTCG698930.23357992575491637No Hit
CCTTGGTATTCTCTACCCGACCACCTGTGTCGGTTTGGGGTACGATTCCT698360.23338943377763638No Hit
CGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGA692530.23144106846615858No Hit
CTGGAGTTCCCACCATTACGTGCTGGCAAACAAGGATAAGGGTTGCGCTC691300.23103000683097544No Hit
GTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGACG688280.2300207335478429No Hit
GCCCACCTTCGTGCTCCTCCGTTACTCTTTGGGAGGAGACCGCCCCAGTC664730.22215040711811707No Hit
CCACCTTCGTGCTCCTCCGTTACTCTTTGGGAGGAGACCGCCCCAGTCAA657730.21981103195853827No Hit
CTCGCAGTTAAGCGGGCTTATGCCATTGCACTAACCTCACGATGTCCAAC631790.21114197601004195No Hit
CCCCAGTCAAACTACCCACCAGGCACTGTCCTCAACCCGGATAACGGGTC629260.2102964589880799No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGC625730.20911674551474946No Hit
CCCATATTCAGACAGGATATCACGTGTCCCGCCCTACTCGATTTCACGCT624120.20857868922804632No Hit
CCTGGAGTTCCCACCATTACGTGCTGGCAAACAAGGATAAGGGTTGCGCT623980.20853190172485472No Hit
GGCTGATCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCT622800.20813754991224No Hit
CGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATT599840.20046439938882152No Hit
CTCCAATCCGGACTACGACGTACTTTGTGAGATTCGCTCCACCTCGCGGT596270.19927131805743634No Hit
GGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCGAC580960.19415477038698614No Hit
CTGGTATCTGCGACTCTCGTCAGCTCCATCCGCGAGGGACTTCACCATCA578750.19341619622946196No Hit
CTGGCACGGAGTTAGCCGGTGCTTCTTCTGTAGGTAACGTCAAATGATTA577610.19303521227490197No Hit
CCTGCATGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCG564930.18879760127155065No Hit
TACGATTCCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCATGGCA562820.18809244675916334No Hit
CCCTGGAGTTCCCACCATTACGTGCTGGCAAACAAGGATAAGGGTTGCGC555500.18564612873514663No Hit
ATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAG552680.18470369474228773No Hit
GGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCGA552040.18448980901341197No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGATCATCCTCTCAGACCAGCT551930.18445304740376145No Hit
CCGACTTCATGGAGTCGAGTTGCAGACTCCAATCCGGACTACGACGTACT547930.18311626159828787No Hit
ACCTGCTTTCGCACCTGCTCGAACCGTCATTCTCGCAGTTAAGCGGGCTT543630.1816792168574037No Hit
GGTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGAC526760.1760413227228188No Hit
CCGGTTTCGGGTCTATATCCAGAGACTGAGCGCCCAGTTAAGACTCGCTT525260.17554002804576618No Hit
CTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGACGACAGCC516770.17270270017364847No Hit
CTTCTCCGTCCCCCCATCGCAATAGTCAGAAGTACGGGAATATTAACCCG508660.16999236695305076No Hit
CACGGAGTTAGCCGGTGCTTCTTCTGTAGGTAACGTCAAATGATTAAGGT507170.16949441424051184No Hit
GCCATGATGACTTGACGTCGTCCCCACCTTCCTCCGGTTTATCACCGGCA506060.1691234561794929No Hit
GGCAAACAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTT505640.16898309366991818No Hit
GTCAGTATCTGTCCAGGGGGCCGCCTTCGCCACCGGTATTCCTTCAGATC497430.1662393408041836No Hit
CTTAGCTGGCGGTCTGGGTTGTTTCCCTCTCCACGACGGACGTTAGCACC497240.16617584347842362No Hit
GCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATT491160.16414392905410374No Hit
GACCTGCTTTCGCACCTGCTCGAACCGTCATTCTCGCAGTTAAGCGGGCT486650.16263670305843225No Hit
CGCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTG485040.1620986467717291No Hit
CGGATCACTATGACCTGCTTTCGCACCTGCTCGAACCGTCATTCTCGCAG479150.16013022967316923No Hit
CCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACC477540.1595921733864661No Hit
AGCACGTGTGTAGCCCTACTCGTAAGGGCCATGATGACTTGACGTCGTCC475300.15884357333540092No Hit
CCCCGGTTTGCTCTGTTAACCTATGTATTCAGTTAACAGTAACTGCTTAT469350.1568551044497589No Hit
CCCCATCGCAATAGTCAGAAGTACGGGAATATTAACCCGTTTCCCATCGA464360.1551874641574306No Hit
TGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTATGACCTGCTTT463960.15505378557688324No Hit
CTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCCGA457540.1529082443590981No Hit
CCGGATCACTATGACCTGCTTTCGCACCTGCTCGAACCGTCATTCTCGCA454170.15178200231798658No Hit
CCCGGTTTGCTCTGTTAACCTATGTATTCAGTTAACAGTAACTGCTTATG431060.14405872232686284No Hit
CCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTT430180.14376462944965865No Hit
GGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGAC428530.14321320530490078No Hit
CTAGCCTTCTCCGTCCCCCCATCGCAATAGTCAGAAGTACGGGAATATTA425500.14220059005725455No Hit
AGCGATTCCGACTTCATGGAGTCGAGTTGCAGACTCCAATCCGGACTACG415650.13890875501127578No Hit
GCCCTCTGTATACGCCATTGTAGCACGTGTGTAGCCCTACTCGTAAGGGC415390.13882186393392No Hit
CTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCTTACCTCACCAA415100.13872494696302318No Hit
GTCGGTTTGGGGTACGATTCCTGACTATCTGAAGCTTAGAGGCTTTTCCT406180.13574391461681704No Hit
CCCCACCTTCCTCCGGTTTATCACCGGCAGTCTCCCTGGAGTTCCCACCA405510.1355200029944002No Hit
GCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGACGACA403410.13481819044652657No Hit
CCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTG402740.13459427882410974No Hit
CTGCCCTCTGTATACGCCATTGTAGCACGTGTGTAGCCCTACTCGTAAGG396920.13264925547714565No Hit
CGTCGCCTTGGTGAGCCCTTACCTCACCAACTAGCTAATCCCACCTGGGC390870.1306273669463668No Hit
CTCTACCCGACCACCTGTGTCGGTTTGGGGTACGATTCCTGACTATCTGA389320.13010936244674579No Hit
CTACTCGTAAGGGCCATGATGACTTGACGTCGTCCCCACCTTCCTCCGGT387210.12940420793435847No Hit
CCTACTCGTAAGGGCCATGATGACTTGACGTCGTCCCCACCTTCCTCCGG384820.128605478415588No Hit
CCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCATGGCATCAATGA377380.1261190568174071No Hit
CGGACTACGACGTACTTTGTGAGATTCGCTCCACCTCGCGGTATCGCTGC374680.1252167263987124No Hit
CTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCC372190.12438457723480509No Hit
GGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGAT371280.12408045846405984No Hit
CACCTGCATGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTC369010.12332183251945357No Hit
GTCGCCTTGGTGAGCCCTTACCTCACCAACTAGCTAATCCCACCTGGGCA367840.12293082267135255No Hit
CCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCCGACCACCTGTGTCG366200.12238274049110837No Hit
CTTGACGTCGTCCCCACCTTCCTCCGGTTTATCACCGGCAGTCTCCCTGG362940.1212932600596474No Hit
GTAGCCCTACTCGTAAGGGCCATGATGACTTGACGTCGTCCCCACCTTCC362780.12123978862742844No Hit
CGGGCAATTAGTACAGGTTAGCTCAACGCCTCACAACGCTTACACACCCT362490.1211428716565316No Hit
CGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGC362330.12108940022431267No Hit
GTCGGTTCGGTCCTCCAGTTGATGTTACTCAACCTTCAACCTGCCCATGG361560.12083206895675898No Hit
CGGCAGTCTCCCTGGAGTTCCCACCATTACGTGCTGGCAAACAAGGATAA356030.11898396258069174No Hit
CTTCAATCTTCCAACGCCCACGGCAGATAGGGACCGAACTGTCTCACGAC353690.11820194288448968No Hit
CGCCTTGGTATTCTCTACCCGACCACCTGTGTCGGTTTGGGGTACGATTC353380.11809834198456548No Hit
CGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTATGACCTGC350900.11726953478517184No Hit
TCTGTATACGCCATTGTAGCACGTGTGTAGCCCTACTCGTAAGGGCCATG350190.11703225530470027No Hit
CCCCCATCGCAATAGTCAGAAGTACGGGAATATTAACCCGTTTCCCATCG348500.11646746330188767No Hit
CTCGATTTCACGCTCGATGAAGCGTCGGTTACGGGGCTATCACCCTGTAT347730.116210132034334No Hit
CCGACATCGAGGTGCCAAACACCGCCGTCGATATGAACTCTTGGGCGGTA347050.11598287844740349No Hit
TGATCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCTTAC346640.11584585790234245No Hit
CCAAGCTGTAGTAAAGGTTCACGGGGTCTTTCCGTCTAGCCGCGGGTACA346240.11571217932179509No Hit
GTGTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTA344880.11525767214793407No Hit
CCCTGCTTTGCTCTTGCGAGGTTATGCGGTATTAGCCATCGTTTCCAATG343990.11496023730621618No Hit
TGGGGTACGATTCCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCA337050.11264091393371949No Hit
CCCTACTCGTAAGGGCCATGATGACTTGACGTCGTCCCCACCTTCCTCCG336610.1124938674951174No Hit
CGGGGTACGATTCCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCA330610.110488688786907No Hit
CCGGACTACGACGTACTTTGTGAGATTCGCTCCACCTCGCGGTATCGCTG328400.10975011462938282No Hit
CTACAGTACTCTAGCTTGTCAGTTTCAAATGCGATTCCTAGGTTGAGCCC327920.10958970033272598No Hit
CGTCATTCTCGCAGTTAAGCGGGCTTATGCCATTGCACTAACCTCACGAT327320.10938918246190495No Hit
CACCTGGTATCTGCGACTCTCGTCAGCTCCATCCGCGAGGGACTTCACCA324220.1083531734626629No Hit
CCCAGTCAAACTACCCACCAGGCACTGTCCTCAACCCAGATAATGGGTCT320970.1072670349957156No Hit
AACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCGACTT316300.10570633756782517No Hit
ATGGAGTCGAGTTGCAGACTCCAATCCGGACTACGACGTACTTTGTGAGA316210.105676259887202No Hit
CGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA315610.10547574201638096No Hit
GGGACCTTAGCTGGCGGTCTGGGTTGTTTCCCTCTCCACGACGGACGTTA310000.10360089992420424No Hit
CCCACCATTACGTGCTGGCAAACAAGGATAAGGGTTGCGCTCGTTGCGGG309090.103296781153459No Hit
TACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTA306940.10257825878301693No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC306230.10234097930254538No Hit
GTCAAACTACCCACCAGGCACTGTCCTCAACCCAGATAATGGGTCTAAGT301810.10086383098749704No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)