FastQCFastQC Report
Wed 6 Sep 2023
6190_GSH1_S10_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename6190_GSH1_S10_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53271619
Sequences flagged as poor quality0
Sequence length200
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCG14226162.6704951467684883No Hit
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC12175232.2855002773615722No Hit
CACGTGTGTAGCCCTACTCGTAAGGGCCATGATGACTTGACGTCGTCCCC6814231.2791482834415075No Hit
CTCAGTTCCAGTGTGGCTGATCATCCTCTCAGACCAGCTAGGGATCGTCG6068091.139084960042232No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATATCA5246870.9849278280804644No Hit
CTGGCAAACAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACAT5106740.9586230146299852No Hit
CCCCTCTACAGTACTCTAGCTTGTCAGTTTCAAATGCGATTCCTAGGTTG3049570.572456789796458No Hit
CCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCA2831570.5315344367513967No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGT2817460.5288857468364159No Hit
CCCACCTTCGTGCTCCTCCGTTACTCTTTGGGAGGAGACCGCCCCAGTCA2680860.5032435751577214No Hit
CCTGCTTTCGCACCTGCTCGAACCGTCATTCTCGCAGTTAAGCGGGCTTA2460570.4618913496884711No Hit
CGATTCCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCATGGCATC2434670.45702947379917247No Hit
CCCCCCTCTACAGTACTCTAGCTTGTCAGTTTCAAATGCGATTCCTAGGT2271260.4263546035647987No Hit
GTACGATTCCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCATGGC2218170.41638869657781563No Hit
CCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCAC2137830.4013074954601999No Hit
TTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAG2119020.39777653463094487No Hit
GTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACT2077950.3900669885779143No Hit
CAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAT2069870.38855023347422574No Hit
CTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTA2043320.3835663413946552No Hit
CTCCGTTACTCTTTGGGAGGAGACCGCCCCAGTCAAACTACCCACCAGGC1999070.375259854595371No Hit
CGTGTCTCAGTTCCAGTGTGGCTGATCATCCTCTCAGACCAGCTAGGGAT1993860.37428184790103713No Hit
GGGTACGATTCCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCATG1987620.3731104924744262No Hit
CCTCTGTATACGCCATTGTAGCACGTGTGTAGCCCTACTCGTAAGGGCCA1845110.34635891205033587No Hit
TGTAGCCCTACTCGTAAGGGCCATGATGACTTGACGTCGTCCCCACCTTC1812870.340306909012846No Hit
CTGGGTTGTTTCCCTCTCCACGACGGACGTTAGCACCCGCCGTGTGTCTC1775290.333252496042968No Hit
CTCGGTTGATTTCTTCTCCTCGGGGTACTTAGATGTTTCAGTTCCCCCGG1765990.3315067259359998No Hit
CTGCTTTCGCACCTGCTCGAACCGTCATTCTCGCAGTTAAGCGGGCTTAT1733000.3253139349866577No Hit
CCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTC1725930.32398677427093026No Hit
TGGCAAACAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATT1714110.3217679567801384No Hit
CCCGGTTTCGGGTCTATATCCAGAGACTGAGCGCCCAGTTAAGACTCGCT1696250.3184153273058962No Hit
CGCCATTGTAGCACGTGTGTAGCCCTACTCGTAAGGGCCATGATGACTTG1659410.31149982507571244No Hit
CGTGTGTAGCCCTACTCGTAAGGGCCATGATGACTTGACGTCGTCCCCAC1651230.30996429825044364No Hit
GTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACA1649840.3097033713204774No Hit
ATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGA1606130.3014982518177268No Hit
CTTGGTATTCTCTACCCGACCACCTGTGTCGGTTTGGGGTACGATTCCTG1595670.29953472974042705No Hit
GCACGTGTGTAGCCCTACTCGTAAGGGCCATGATGACTTGACGTCGTCCC1555300.2919565857384586No Hit
CTGGAGTTCCCACCATTACGTGCTGGCAAACAAGGATAAGGGTTGCGCTC1551670.2912751722450936No Hit
GTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGACG1529800.28716979673548126No Hit
CCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTG1507500.2829837028230736No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATATCACGTGT1493070.2802749433990358No Hit
CCGTGTCTCAGTTCCAGTGTGGCTGATCATCCTCTCAGACCAGCTAGGGA1474480.27678528035725736No Hit
CCCCAGTCAAACTACCCACCAGGCACTGTCCTCAACCCAGATAATGGGTC1473380.27657879141987407No Hit
CTAAGTTAGAACATCAAACATACAAGGGTGGTATTTCAAGGACGGCTCCA1471740.2762709351859571No Hit
GTGTAGCCCTACTCGTAAGGGCCATGATGACTTGACGTCGTCCCCACCTT1458360.2737592788385125No Hit
CCTGGAGTTCCCACCATTACGTGCTGGCAAACAAGGATAAGGGTTGCGCT1417970.26617738049222794No Hit
CTCTGTATACGCCATTGTAGCACGTGTGTAGCCCTACTCGTAAGGGCCAT1382480.2595152965033783No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT1369950.25716319978936625No Hit
CTCTACAGTACTCTAGCTTGTCAGTTTCAAATGCGATTCCTAGGTTGAGC1340810.25169312012086587No Hit
CACCGGATCACTATGACCTGCTTTCGCACCTGCTCGAACCGTCATTCTCG1338030.25117126626093345No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT1336780.25093661974117965No Hit
GGCTAATCCAGGTTCGCTCGCCGCTACTACCGGAATCTCGGTTGATTTCT1329700.24960758185329415No Hit
CTCGCAGTTAAGCGGGCTTATGCCATTGCACTAACCTCACGATGTCCAAC1309610.2458363429878112No Hit
CCCTGGAGTTCCCACCATTACGTGCTGGCAAACAAGGATAAGGGTTGCGC1251090.23485113151901765No Hit
CCTTGGTATTCTCTACCCGACCACCTGTGTCGGTTTGGGGTACGATTCCT1233880.23162051823504745No Hit
CCCATATTCAGACAGGATATCACGTGTCCCGCCCTACTCGATTTCACGCT1232300.23132392503407867No Hit
CTCCAATCCGGACTACGACGTACTTTGTGAGATTCGCTCCACCTCGCGGT1224330.2298278188241285No Hit
ATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAG1208100.22678116841164525No Hit
GCCCACCTTCGTGCTCCTCCGTTACTCTTTGGGAGGAGACCGCCCCAGTC1156230.21704427642794186No Hit
GGTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGAC1154880.21679085818660776No Hit
GTTGATTTCTTCTCCTCGGGGTACTTAGATGTTTCAGTTCCCCCGGTTTG1147950.21548997788109273No Hit
GGCAAACAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTT1146410.21520089336875609No Hit
CGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGA1142980.21455702331855167No Hit
CCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGG1138450.2137066643309639No Hit
GGCTGATCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCT1134680.2129989704273865No Hit
CCACCTTCGTGCTCCTCCGTTACTCTTTGGGAGGAGACCGCCCCAGTCAA1127490.2116492836457627No Hit
CTTAGCTGGCGGTCTGGGTTGTTTCCCTCTCCACGACGGACGTTAGCACC1108150.208018832692132No Hit
TACGATTCCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCATGGCA1077960.2023516499470384No Hit
GGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCGAC1068140.2005082668878526No Hit
CCCCAGTCAAACTACCCACCAGGCACTGTCCTCAACCCGGATAACGGGTC1066640.20022669106414806No Hit
CTGGTATCTGCGACTCTCGTCAGCTCCATCCGCGAGGGACTTCACCATCA1065850.20007839446366366No Hit
CTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGACGACAGCC1063310.19960159273552397No Hit
CCGACTTCATGGAGTCGAGTTGCAGACTCCAATCCGGACTACGACGTACT1045100.19618326223575072No Hit
CCGGTTTCGGGTCTATATCCAGAGACTGAGCGCCCAGTTAAGACTCGCTT1043970.1959711417818933No Hit
GCCATGATGACTTGACGTCGTCCCCACCTTCCTCCGGTTTATCACCGGCA1018200.1911336691306491No Hit
CTTCTCCGTCCCCCCATCGCAATAGTCAGAAGTACGGGAATATTAACCCG1014930.19051983383497317No Hit
GGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCGA1001000.1879049330188369No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGATCATCCTCTCAGACCAGCT999850.18768905822066342No Hit
CTGGCACGGAGTTAGCCGGTGCTTCTTCTGTAGGTAACGTCAAATGATTA990870.18600335762275216No Hit
CAAGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGC968780.18185668432566315No Hit
CGGATCACTATGACCTGCTTTCGCACCTGCTCGAACCGTCATTCTCGCAG962360.18065153980020768No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGC959380.18009214249711464No Hit
CCTGCATGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCG945930.17756734594456383No Hit
ACCTGCTTTCGCACCTGCTCGAACCGTCATTCTCGCAGTTAAGCGGGCTT941670.1767676706052429No Hit
CGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCAACATTCTGATT940220.1764954806423285No Hit
AGCACGTGTGTAGCCCTACTCGTAAGGGCCATGATGACTTGACGTCGTCC926410.17390310589208863No Hit
CGCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTG914260.17162234172008176No Hit
CCGGATCACTATGACCTGCTTTCGCACCTGCTCGAACCGTCATTCTCGCA887270.1665558540655579No Hit
CACGGAGTTAGCCGGTGCTTCTTCTGTAGGTAACGTCAAATGATTAAGGT872130.16371381541829994No Hit
CTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCCGA869100.16314503225441673No Hit
CCCCATCGCAATAGTCAGAAGTACGGGAATATTAACCCGTTTCCCATCGA861970.16180660850574113No Hit
GACCTGCTTTCGCACCTGCTCGAACCGTCATTCTCGCAGTTAAGCGGGCT850750.15970042134443108No Hit
CCCCACCTTCCTCCGGTTTATCACCGGCAGTCTCCCTGGAGTTCCCACCA850720.15969478982795698No Hit
CCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACC845450.15870552010067498No Hit
GCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATT841620.15798656316414939No Hit
GTCAGTATCTGTCCAGGGGGCCGCCTTCGCCACCGGTATTCCTTCAGATC832850.15634028318155677No Hit
AGCGATTCCGACTTCATGGAGTCGAGTTGCAGACTCCAATCCGGACTACG832570.15628772236113192No Hit
CTAGCCTTCTCCGTCCCCCCATCGCAATAGTCAGAAGTACGGGAATATTA832480.15627082781170964No Hit
TGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTATGACCTGCTTT819510.1538361355227443No Hit
CGGCAGTCTCCCTGGAGTTCCCACCATTACGTGCTGGCAAACAAGGATAA802050.15055859293482332No Hit
GCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGACGACA799970.150168141125953No Hit
CCAAGCTGTAGTAAAGGTTCACGGGGTCTTTCCGTCTAGCCGCGGGTACA790200.1483341439275574No Hit
CCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCATGGCATCAATGA776300.1457248746278952No Hit
CTCTACCCGACCACCTGTGTCGGTTTGGGGTACGATTCCTGACTATCTGA769910.14452536161891383No Hit
CTCGATTTCACGCTCGATGAAGCGTCGGTTACGGGGCTATCACCCTGTAT767880.14414429567083364No Hit
CGGACTACGACGTACTTTGTGAGATTCGCTCCACCTCGCGGTATCGCTGC765560.14370879173017062No Hit
CGTCGCCTTGGTGAGCCCTTACCTCACCAACTAGCTAATCCCACCTGGGC760480.14275518827389122No Hit
GTCGGTTTGGGGTACGATTCCTGACTATCTGAAGCTTAGAGGCTTTTCCT738360.1386028834603281No Hit
CTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCTTACCTCACCAA734350.13785013742495794No Hit
CTGCCCTCTGTATACGCCATTGTAGCACGTGTGTAGCCCTACTCGTAAGG733790.13774501578410822No Hit
GCCCTCTGTATACGCCATTGTAGCACGTGTGTAGCCCTACTCGTAAGGGC733660.13772061254605383No Hit
CTTGACGTCGTCCCCACCTTCCTCCGGTTTATCACCGGCAGTCTCCCTGG723910.13589036969197427No Hit
GTAGCCCTACTCGTAAGGGCCATGATGACTTGACGTCGTCCCCACCTTCC713550.13394561933625485No Hit
CCCACCATTACGTGCTGGCAAACAAGGATAAGGGTTGCGCTCGTTGCGGG711370.13353639580580423No Hit
GTCGCCTTGGTGAGCCCTTACCTCACCAACTAGCTAATCCCACCTGGGCA703390.13203841242369602No Hit
CCTACTCGTAAGGGCCATGATGACTTGACGTCGTCCCCACCTTCCTCCGG701640.1317099072960407No Hit
ATGGAGTCGAGTTGCAGACTCCAATCCGGACTACGACGTACTTTGTGAGA698550.13112986109920932No Hit
CTACTCGTAAGGGCCATGATGACTTGACGTCGTCCCCACCTTCCTCCGGT694310.13033394010420446No Hit
GTCGGTTCGGTCCTCCAGTTGATGTTACTCAACCTTCAACCTGCCCATGG687310.12901991959358322No Hit
CCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCCGACCACCTGTGTCG685060.12859755585802637No Hit
CCGACATCGAGGTGCCAAACACCGCCGTCGATATGAACTCTTGGGCGGTA680930.12782228375675986No Hit
GGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGAC678590.12738302547178076No Hit
CGTCATTCTCGCAGTTAAGCGGGCTTATGCCATTGCACTAACCTCACGAT677270.12713523874692076No Hit
CCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTG676710.12703011710607107No Hit
CCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTT674010.12652328062340285No Hit
CTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCC668660.12551899351885665No Hit
CCGGACTACGACGTACTTTGTGAGATTCGCTCCACCTCGCGGTATCGCTG658470.12360615508982373No Hit
TCTGTATACGCCATTGTAGCACGTGTGTAGCCCTACTCGTAAGGGCCATG658220.12355922578587296No Hit
CGCCTTGGTATTCTCTACCCGACCACCTGTGTCGGTTTGGGGTACGATTC653690.12270886679828523No Hit
CCCCGGTTTGCTCTGTTAACCTATGTATTCAGTTAACAGTAACTGCTTAT637270.11962655011479939No Hit
CCCCCATCGCAATAGTCAGAAGTACGGGAATATTAACCCGTTTCCCATCG632630.11875554223347333No Hit
CCCTACTCGTAAGGGCCATGATGACTTGACGTCGTCCCCACCTTCCTCCG631050.11845894903250453No Hit
TGGGGTACGATTCCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCA628830.1180422168134218No Hit
CAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAAC625600.11743589020637799No Hit
CGGGGTACGATTCCTGACTATCTGAAGCTTAGAGGCTTTTCCTGGAAGCA620520.11648228675009858No Hit
TGATCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCTTAC618520.11610685231849252No Hit
CCCTGCTTTGCTCTTGCGAGGTTATGCGGTATTAGCCATCGTTTCCAATG617740.11596043289016617No Hit
CCTGGAAGCATGGCATCAATGACTTCACTACCGTAGTAGCTCGACGTCGT616340.1156976287880419No Hit
CTGGAAGCATGGCATCAATGACTTCACTACCGTAGTAGCTCGACGTCGTG615710.115579366942086No Hit
GGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGAT606550.1138598772453302No Hit
CGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGACGACAG604790.11352949494551685No Hit
CCCGGTTTGCTCTGTTAACCTATGTATTCAGTTAACAGTAACTGCTTATG602350.11307146493895745No Hit
GGGACCTTAGCTGGCGGTCTGGGTTGTTTCCCTCTCCACGACGGACGTTA596540.11198082791514183No Hit
CACCTGGTATCTGCGACTCTCGTCAGCTCCATCCGCGAGGGACTTCACCA592210.1111680123707147No Hit
CACCTGCATGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTC592180.1111623808542406No Hit
CGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTATGACCTGC580780.10902240459408602No Hit
AACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCGACTT568980.10680734144761023No Hit
TGATGACTTGACGTCGTCCCCACCTTCCTCCGGTTTATCACCGGCAGTCT566670.10637371467910521No Hit
CGGCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGA561840.10546704052677655No Hit
GTTTTTAATAAACAGTTGCAGCCACCTGGTATCTGCGACTCTCGTCAGCT552320.10367997263233167No Hit
CCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCCGACCACCTGTGTCGG551670.1035579564420597No Hit
CGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGC549560.1031618731167153No Hit
CCCAGTCAAACTACCCACCAGGCACTGTCCTCAACCCAGATAATGGGTCT542970.1019248166645733No Hit
ATTTCAAGGACGGCTCCAACGCAACTGGCGTCACGTCTTCATAGCCTCCC539490.10127156075357875No Hit
TCCGACTTCATGGAGTCGAGTTGCAGACTCCAATCCGGACTACGACGTAC536930.100791004681123No Hit
CTATGACCTGCTTTCGCACCTGCTCGAACCGTCATTCTCGCAGTTAAGCG535820.1005826385715816No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC535750.1005694983664754No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)