Detecting adapter sequence for read1... >I7_Nextera_Transposase_1 | >Trans2_rc | >I7_Nextera_Transposase_1 | >Trans2_rc CTGTCTCTTATACACATCTCCGAGCCCACGAGAC Read1 before filtering: total reads: 2992492 total bases: 362091532 Q20 bases: 316159351(87.3148%) Q30 bases: 249919279(69.021%) Read1 after filtering: total reads: 2152057 total bases: 146045677 Q20 bases: 134563065(92.1377%) Q30 bases: 119176971(81.6025%) Filtering result: reads passed filter: 2152057 reads failed due to low quality: 68506 reads failed due to too many N: 5 reads failed due to too short: 771924 reads with adapter trimmed: 2253328 bases trimmed due to adapters: 143308920 Duplication rate (may be overestimated since this is SE data): 34.9058% JSON report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_001.json HTML report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_001.html /userapps/hb-molgen/fastp/fastp -i /scratch/hb-molgen/NGSdata/20250702_024/Anne/AJ024_001_S19_L001_R1_001.fastq.gz -o /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_001.trimmed_tail.fastq.gz --trim_tail1 20 --html /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_001.html --json /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_001.json fastp v0.26.0, time used: 4 seconds Detecting adapter sequence for read1... >I7_Nextera_Transposase_1 | >Trans2_rc | >I7_Nextera_Transposase_1 | >Trans2_rc CTGTCTCTTATACACATCTCCGAGCCCACGAGAC Read1 before filtering: total reads: 3978412 total bases: 481387852 Q20 bases: 420396426(87.3301%) Q30 bases: 332450758(69.0609%) Read1 after filtering: total reads: 2836549 total bases: 186849701 Q20 bases: 172971813(92.5727%) Q30 bases: 154340977(82.6017%) Filtering result: reads passed filter: 2836549 reads failed due to low quality: 86031 reads failed due to too many N: 5 reads failed due to too short: 1055827 reads with adapter trimmed: 2964124 bases trimmed due to adapters: 197735602 Duplication rate (may be overestimated since this is SE data): 38.273% JSON report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_002.json HTML report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_002.html /userapps/hb-molgen/fastp/fastp -i /scratch/hb-molgen/NGSdata/20250702_024/Anne/AJ024_002_S20_L001_R1_001.fastq.gz -o /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_002.trimmed_tail.fastq.gz --trim_tail1 20 --html /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_002.html --json /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_002.json fastp v0.26.0, time used: 6 seconds Detecting adapter sequence for read1... >I7_Nextera_Transposase_1 | >Trans2_rc | >I7_Nextera_Transposase_1 | >Trans2_rc CTGTCTCTTATACACATCTCCGAGCCCACGAGAC Read1 before filtering: total reads: 4035229 total bases: 488262709 Q20 bases: 432401647(88.5592%) Q30 bases: 350204908(71.7247%) Read1 after filtering: total reads: 3068600 total bases: 199501490 Q20 bases: 184998173(92.7302%) Q30 bases: 165343361(82.8783%) Filtering result: reads passed filter: 3068600 reads failed due to low quality: 88775 reads failed due to too many N: 9 reads failed due to too short: 877845 reads with adapter trimmed: 3146605 bases trimmed due to adapters: 191414213 Duplication rate (may be overestimated since this is SE data): 39.3887% JSON report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_003.json HTML report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_003.html /userapps/hb-molgen/fastp/fastp -i /scratch/hb-molgen/NGSdata/20250702_024/Anne/AJ024_003_S21_L001_R1_001.fastq.gz -o /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_003.trimmed_tail.fastq.gz --trim_tail1 20 --html /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_003.html --json /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_003.json fastp v0.26.0, time used: 6 seconds Detecting adapter sequence for read1... >I7_Nextera_Transposase_1 | >Trans2_rc | >I7_Nextera_Transposase_1 | >Trans2_rc CTGTCTCTTATACACATCTCCGAGCCCACGAGAC Read1 before filtering: total reads: 759629 total bases: 91915109 Q20 bases: 82522930(89.7817%) Q30 bases: 67850770(73.819%) Read1 after filtering: total reads: 549089 total bases: 34914662 Q20 bases: 32812948(93.9804%) Q30 bases: 29884695(85.5935%) Filtering result: reads passed filter: 549089 reads failed due to low quality: 11701 reads failed due to too many N: 2 reads failed due to too short: 198837 reads with adapter trimmed: 595586 bases trimmed due to adapters: 39033056 Duplication rate (may be overestimated since this is SE data): 49.2558% JSON report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_004.json HTML report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_004.html /userapps/hb-molgen/fastp/fastp -i /scratch/hb-molgen/NGSdata/20250702_024/Anne/AJ024_004_S22_L001_R1_001.fastq.gz -o /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_004.trimmed_tail.fastq.gz --trim_tail1 20 --html /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_004.html --json /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_004.json fastp v0.26.0, time used: 2 seconds Detecting adapter sequence for read1... >I7_Nextera_Transposase_1 | >Trans2_rc | >I7_Nextera_Transposase_1 | >Trans2_rc CTGTCTCTTATACACATCTCCGAGCCCACGAGAC Read1 before filtering: total reads: 1871369 total bases: 226435649 Q20 bases: 208274009(91.9793%) Q30 bases: 178569688(78.8611%) Read1 after filtering: total reads: 1501319 total bases: 100462871 Q20 bases: 96708486(96.2629%) Q30 bases: 90760351(90.3422%) Filtering result: reads passed filter: 1501319 reads failed due to low quality: 22416 reads failed due to too many N: 3 reads failed due to too short: 347631 reads with adapter trimmed: 1357773 bases trimmed due to adapters: 83575504 Duplication rate (may be overestimated since this is SE data): 43.9787% JSON report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_005.json HTML report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_005.html /userapps/hb-molgen/fastp/fastp -i /scratch/hb-molgen/NGSdata/20250702_024/Anne/AJ024_005_S23_L001_R1_001.fastq.gz -o /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_005.trimmed_tail.fastq.gz --trim_tail1 20 --html /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_005.html --json /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_005.json fastp v0.26.0, time used: 3 seconds Detecting adapter sequence for read1... >I7_Nextera_Transposase_1 | >Trans2_rc | >I7_Nextera_Transposase_1 | >Trans2_rc CTGTCTCTTATACACATCTCCGAGCCCACGAGAC Read1 before filtering: total reads: 7984980 total bases: 966182580 Q20 bases: 859771076(88.9864%) Q30 bases: 705952896(73.0662%) Read1 after filtering: total reads: 6364471 total bases: 412391768 Q20 bases: 386992950(93.8411%) Q30 bases: 352122255(85.3854%) Filtering result: reads passed filter: 6364471 reads failed due to low quality: 130274 reads failed due to too many N: 14 reads failed due to too short: 1490221 reads with adapter trimmed: 6022053 bases trimmed due to adapters: 368997117 Duplication rate (may be overestimated since this is SE data): 42.2512% JSON report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_006.json HTML report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_006.html /userapps/hb-molgen/fastp/fastp -i /scratch/hb-molgen/NGSdata/20250702_024/Anne/AJ024_006_S24_L001_R1_001.fastq.gz -o /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_006.trimmed_tail.fastq.gz --trim_tail1 20 --html /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_006.html --json /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_006.json fastp v0.26.0, time used: 12 seconds Detecting adapter sequence for read1... >I7_Nextera_Transposase_1 | >Trans2_rc | >I7_Nextera_Transposase_1 | >Trans2_rc CTGTCTCTTATACACATCTCCGAGCCCACGAGAC Read1 before filtering: total reads: 5428420 total bases: 656838820 Q20 bases: 639984910(97.4341%) Q30 bases: 600515351(91.4251%) Read1 after filtering: total reads: 5074627 total bases: 418330053 Q20 bases: 413310837(98.8002%) Q30 bases: 398048804(95.1519%) Filtering result: reads passed filter: 5074627 reads failed due to low quality: 28939 reads failed due to too many N: 7 reads failed due to too short: 324847 reads with adapter trimmed: 2484985 bases trimmed due to adapters: 124313915 Duplication rate (may be overestimated since this is SE data): 44.3006% JSON report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_007.json HTML report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_007.html /userapps/hb-molgen/fastp/fastp -i /scratch/hb-molgen/NGSdata/20250702_024/Anne/AJ024_007_S25_L001_R1_001.fastq.gz -o /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_007.trimmed_tail.fastq.gz --trim_tail1 20 --html /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_007.html --json /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_007.json fastp v0.26.0, time used: 10 seconds Detecting adapter sequence for read1... >I7_Nextera_Transposase_1 | >Trans2_rc | >I7_Nextera_Transposase_1 | >Trans2_rc CTGTCTCTTATACACATCTCCGAGCCCACGAGAC Read1 before filtering: total reads: 5417954 total bases: 655572434 Q20 bases: 631480166(96.325%) Q30 bases: 583503222(89.0067%) Read1 after filtering: total reads: 4903803 total bases: 373683283 Q20 bases: 367277674(98.2858%) Q30 bases: 352567646(94.3493%) Filtering result: reads passed filter: 4903803 reads failed due to low quality: 33752 reads failed due to too many N: 3 reads failed due to too short: 480396 reads with adapter trimmed: 3135745 bases trimmed due to adapters: 166082570 Duplication rate (may be overestimated since this is SE data): 66.9098% JSON report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_008.json HTML report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_008.html /userapps/hb-molgen/fastp/fastp -i /scratch/hb-molgen/NGSdata/20250702_024/Anne/AJ024_008_S26_L001_R1_001.fastq.gz -o /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_008.trimmed_tail.fastq.gz --trim_tail1 20 --html /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_008.html --json /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_008.json fastp v0.26.0, time used: 8 seconds Detecting adapter sequence for read1... >Nextera_LMP_Read1_External_Adapter | >Illumina Multiplexing Index Sequencing Primer GATCGGAAGAGCACACGTCTGAACTCCAGTCAC Read1 before filtering: total reads: 7876650 total bases: 953074650 Q20 bases: 785411404(82.4082%) Q30 bases: 555175115(58.251%) Read1 after filtering: total reads: 6723920 total bases: 511170378 Q20 bases: 446208097(87.2915%) Q30 bases: 351013155(68.6685%) Filtering result: reads passed filter: 6723920 reads failed due to low quality: 786539 reads failed due to too many N: 16 reads failed due to too short: 366175 reads with adapter trimmed: 3526995 bases trimmed due to adapters: 203724489 Duplication rate (may be overestimated since this is SE data): 32.6357% JSON report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_009.json HTML report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_009.html /userapps/hb-molgen/fastp/fastp -i /scratch/hb-molgen/NGSdata/20250702_024/Anne/AJ024_009_S27_L001_R1_001.fastq.gz -o /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_009.trimmed_tail.fastq.gz --trim_tail1 20 --html /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_009.html --json /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_009.json fastp v0.26.0, time used: 15 seconds Detecting adapter sequence for read1... >Nextera_LMP_Read1_External_Adapter | >Illumina Multiplexing Index Sequencing Primer GATCGGAAGAGCACACGTCTGAACTCCAGTCAC Read1 before filtering: total reads: 5366556 total bases: 649353276 Q20 bases: 562696210(86.6549%) Q30 bases: 429634207(66.1634%) Read1 after filtering: total reads: 4538765 total bases: 344868262 Q20 bases: 314836509(91.2918%) Q30 bases: 260442944(75.5195%) Filtering result: reads passed filter: 4538765 reads failed due to low quality: 277853 reads failed due to too many N: 13 reads failed due to too short: 549925 reads with adapter trimmed: 2549789 bases trimmed due to adapters: 165120296 Duplication rate (may be overestimated since this is SE data): 49.1141% JSON report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_010.json HTML report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_010.html /userapps/hb-molgen/fastp/fastp -i /scratch/hb-molgen/NGSdata/20250702_024/Anne/AJ024_010_S28_L001_R1_001.fastq.gz -o /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_010.trimmed_tail.fastq.gz --trim_tail1 20 --html /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_010.html --json /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_010.json fastp v0.26.0, time used: 9 seconds Detecting adapter sequence for read1... >Nextera_LMP_Read1_External_Adapter | >Illumina Multiplexing Index Sequencing Primer GATCGGAAGAGCACACGTCTGAACTCCAGTCAC Read1 before filtering: total reads: 4817498 total bases: 582917258 Q20 bases: 505202548(86.668%) Q30 bases: 383976372(65.8715%) Read1 after filtering: total reads: 4200997 total bases: 325237643 Q20 bases: 295449692(90.8412%) Q30 bases: 240520639(73.9523%) Filtering result: reads passed filter: 4200997 reads failed due to low quality: 219100 reads failed due to too many N: 2 reads failed due to too short: 397399 reads with adapter trimmed: 2197404 bases trimmed due to adapters: 135948857 Duplication rate (may be overestimated since this is SE data): 47.9064% JSON report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_011.json HTML report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_011.html /userapps/hb-molgen/fastp/fastp -i /scratch/hb-molgen/NGSdata/20250702_024/Anne/AJ024_011_S29_L001_R1_001.fastq.gz -o /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_011.trimmed_tail.fastq.gz --trim_tail1 20 --html /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_011.html --json /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_011.json fastp v0.26.0, time used: 8 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Read1 before filtering: total reads: 2293180 total bases: 277474780 Q20 bases: 236310263(85.1646%) Q30 bases: 178237260(64.2355%) Read1 after filtering: total reads: 2057993 total bases: 137260059 Q20 bases: 123934983(90.2921%) Q30 bases: 103933808(75.7204%) Filtering result: reads passed filter: 2057993 reads failed due to low quality: 73824 reads failed due to too many N: 7 reads failed due to too short: 161356 reads with adapter trimmed: 1337059 bases trimmed due to adapters: 78651592 Duplication rate (may be overestimated since this is SE data): 54.9999% JSON report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_012.json HTML report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_012.html /userapps/hb-molgen/fastp/fastp -i /scratch/hb-molgen/NGSdata/20250702_024/Anne/AJ024_012_S30_L001_R1_001.fastq.gz -o /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_012.trimmed_tail.fastq.gz --trim_tail1 20 --html /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_012.html --json /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_012.json fastp v0.26.0, time used: 4 seconds Detecting adapter sequence for read1... >Nextera_LMP_Read1_External_Adapter | >Illumina Multiplexing Index Sequencing Primer GATCGGAAGAGCACACGTCTGAACTCCAGTCAC Read1 before filtering: total reads: 1970901 total bases: 238479021 Q20 bases: 209228305(87.7345%) Q30 bases: 166324332(69.7438%) Read1 after filtering: total reads: 1785622 total bases: 134163502 Q20 bases: 123960454(92.3951%) Q30 bases: 106401774(79.3075%) Filtering result: reads passed filter: 1785622 reads failed due to low quality: 61501 reads failed due to too many N: 2 reads failed due to too short: 123776 reads with adapter trimmed: 973039 bases trimmed due to adapters: 57628484 Duplication rate (may be overestimated since this is SE data): 45.0138% JSON report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_013.json HTML report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_013.html /userapps/hb-molgen/fastp/fastp -i /scratch/hb-molgen/NGSdata/20250702_024/Anne/AJ024_013_S31_L001_R1_001.fastq.gz -o /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_013.trimmed_tail.fastq.gz --trim_tail1 20 --html /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_013.html --json /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_013.json fastp v0.26.0, time used: 4 seconds Detecting adapter sequence for read1... >Nextera_LMP_Read1_External_Adapter | >Illumina Multiplexing Index Sequencing Primer GATCGGAAGAGCACACGTCTGAACTCCAGTCAC Read1 before filtering: total reads: 9245403 total bases: 1118693763 Q20 bases: 970550657(86.7575%) Q30 bases: 757375049(67.7017%) Read1 after filtering: total reads: 8170868 total bases: 618148307 Q20 bases: 566034188(91.5693%) Q30 bases: 478386458(77.3902%) Filtering result: reads passed filter: 8170868 reads failed due to low quality: 381845 reads failed due to too many N: 21 reads failed due to too short: 692669 reads with adapter trimmed: 4613619 bases trimmed due to adapters: 271627348 Duplication rate (may be overestimated since this is SE data): 46.7479% JSON report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_014.json HTML report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_014.html /userapps/hb-molgen/fastp/fastp -i /scratch/hb-molgen/NGSdata/20250702_024/Anne/AJ024_014_S32_L001_R1_001.fastq.gz -o /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_014.trimmed_tail.fastq.gz --trim_tail1 20 --html /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_014.html --json /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_014.json fastp v0.26.0, time used: 16 seconds Detecting adapter sequence for read1... >Nextera_LMP_Read1_External_Adapter | >Illumina Multiplexing Index Sequencing Primer GATCGGAAGAGCACACGTCTGAACTCCAGTCAC Read1 before filtering: total reads: 5356178 total bases: 648097538 Q20 bases: 604665354(93.2985%) Q30 bases: 533314686(82.2893%) Read1 after filtering: total reads: 5127601 total bases: 453867538 Q20 bases: 436222068(96.1122%) Q30 bases: 400086428(88.1505%) Filtering result: reads passed filter: 5127601 reads failed due to low quality: 57516 reads failed due to too many N: 12 reads failed due to too short: 171049 reads with adapter trimmed: 1786693 bases trimmed due to adapters: 79720007 Duplication rate (may be overestimated since this is SE data): 39.899% JSON report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_015.json HTML report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_015.html /userapps/hb-molgen/fastp/fastp -i /scratch/hb-molgen/NGSdata/20250702_024/Anne/AJ024_015_S33_L001_R1_001.fastq.gz -o /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_015.trimmed_tail.fastq.gz --trim_tail1 20 --html /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_015.html --json /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_015.json fastp v0.26.0, time used: 11 seconds Detecting adapter sequence for read1... >Nextera_LMP_Read1_External_Adapter | >Illumina Multiplexing Index Sequencing Primer GATCGGAAGAGCACACGTCTGAACTCCAGTCAC Read1 before filtering: total reads: 4166194 total bases: 504109474 Q20 bases: 453770277(90.0142%) Q30 bases: 382093631(75.7958%) Read1 after filtering: total reads: 3827762 total bases: 322587849 Q20 bases: 303881859(94.2013%) Q30 bases: 271546794(84.1776%) Filtering result: reads passed filter: 3827762 reads failed due to low quality: 102668 reads failed due to too many N: 8 reads failed due to too short: 235756 reads with adapter trimmed: 1688938 bases trimmed due to adapters: 85829065 Duplication rate (may be overestimated since this is SE data): 45.3697% JSON report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_016.json HTML report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_016.html /userapps/hb-molgen/fastp/fastp -i /scratch/hb-molgen/NGSdata/20250702_024/Anne/AJ024_016_S34_L001_R1_001.fastq.gz -o /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_016.trimmed_tail.fastq.gz --trim_tail1 20 --html /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_016.html --json /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/AJ024_016.json fastp v0.26.0, time used: 8 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Read1 before filtering: total reads: 5128655 total bases: 620567255 Q20 bases: 607148328(97.8376%) Q30 bases: 576368986(92.8778%) Read1 after filtering: total reads: 5113017 total bases: 464488130 Q20 bases: 459504829(98.9271%) Q30 bases: 442047330(95.1687%) Filtering result: reads passed filter: 5113017 reads failed due to low quality: 11161 reads failed due to too many N: 23 reads failed due to too short: 4454 reads with adapter trimmed: 1469499 bases trimmed due to adapters: 52146353 Duplication rate (may be overestimated since this is SE data): 14.5055% JSON report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/CT024_001.json HTML report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/CT024_001.html /userapps/hb-molgen/fastp/fastp -i /scratch/hb-molgen/NGSdata/20250702_024/Anne/CT024_001_S35_L001_R1_001.fastq.gz -o /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/CT024_001.trimmed_tail.fastq.gz --trim_tail1 20 --html /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/CT024_001.html --json /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/CT024_001.json fastp v0.26.0, time used: 12 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Read1 before filtering: total reads: 8070902 total bases: 976579142 Q20 bases: 951077894(97.3887%) Q30 bases: 897201918(91.8719%) Read1 after filtering: total reads: 8048310 total bases: 691616544 Q20 bases: 683982659(98.8962%) Q30 bases: 657115803(95.0116%) Filtering result: reads passed filter: 8048310 reads failed due to low quality: 19997 reads failed due to too many N: 17 reads failed due to too short: 2578 reads with adapter trimmed: 3526314 bases trimmed due to adapters: 121524336 Duplication rate (may be overestimated since this is SE data): 12.3159% JSON report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/CT024_002.json HTML report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/CT024_002.html /userapps/hb-molgen/fastp/fastp -i /scratch/hb-molgen/NGSdata/20250702_024/Anne/CT024_002_S36_L001_R1_001.fastq.gz -o /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/CT024_002.trimmed_tail.fastq.gz --trim_tail1 20 --html /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/CT024_002.html --json /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/CT024_002.json fastp v0.26.0, time used: 16 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Read1 before filtering: total reads: 6632042 total bases: 802477082 Q20 bases: 781619112(97.4008%) Q30 bases: 738545996(92.0333%) Read1 after filtering: total reads: 6615480 total bases: 565903691 Q20 bases: 560046245(98.9649%) Q30 bases: 539314912(95.3015%) Filtering result: reads passed filter: 6615480 reads failed due to low quality: 14380 reads failed due to too many N: 11 reads failed due to too short: 2171 reads with adapter trimmed: 2933463 bases trimmed due to adapters: 102456850 Duplication rate (may be overestimated since this is SE data): 10.5041% JSON report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/CT024_003.json HTML report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/CT024_003.html /userapps/hb-molgen/fastp/fastp -i /scratch/hb-molgen/NGSdata/20250702_024/Anne/CT024_003_S37_L001_R1_001.fastq.gz -o /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/CT024_003.trimmed_tail.fastq.gz --trim_tail1 20 --html /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/CT024_003.html --json /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/CT024_003.json fastp v0.26.0, time used: 14 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Read1 before filtering: total reads: 6676653 total bases: 807875013 Q20 bases: 789876845(97.7722%) Q30 bases: 749356775(92.7565%) Read1 after filtering: total reads: 6652040 total bases: 603271652 Q20 bases: 596363233(98.8548%) Q30 bases: 572913141(94.9677%) Filtering result: reads passed filter: 6652040 reads failed due to low quality: 21428 reads failed due to too many N: 15 reads failed due to too short: 3170 reads with adapter trimmed: 2093212 bases trimmed due to adapters: 68795246 Duplication rate (may be overestimated since this is SE data): 14.139% JSON report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/CT024_004.json HTML report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/CT024_004.html /userapps/hb-molgen/fastp/fastp -i /scratch/hb-molgen/NGSdata/20250702_024/Anne/CT024_004_S38_L001_R1_001.fastq.gz -o /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/CT024_004.trimmed_tail.fastq.gz --trim_tail1 20 --html /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/CT024_004.html --json /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/CT024_004.json fastp v0.26.0, time used: 15 seconds Detecting adapter sequence for read1... >Nextera_LMP_Read1_External_Adapter | >Illumina Multiplexing Index Sequencing Primer GATCGGAAGAGCACACGTCTGAACTCCAGTCAC Read1 before filtering: total reads: 4085419 total bases: 494335699 Q20 bases: 486496312(98.4142%) Q30 bases: 466158684(94.3%) Read1 after filtering: total reads: 3998346 total bases: 389799865 Q20 bases: 385979071(99.0198%) Q30 bases: 372076417(95.4532%) Filtering result: reads passed filter: 3998346 reads failed due to low quality: 33638 reads failed due to too many N: 22 reads failed due to too short: 53413 reads with adapter trimmed: 635885 bases trimmed due to adapters: 19369215 Duplication rate (may be overestimated since this is SE data): 49.0687% JSON report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_001.json HTML report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_001.html /userapps/hb-molgen/fastp/fastp -i /scratch/hb-molgen/NGSdata/20250702_024/Anne/DS024_001_S1_L001_R1_001.fastq.gz -o /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_001.trimmed_tail.fastq.gz --trim_tail1 20 --html /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_001.html --json /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_001.json fastp v0.26.0, time used: 10 seconds Detecting adapter sequence for read1... >Nextera_LMP_Read1_External_Adapter | >Illumina Multiplexing Index Sequencing Primer GATCGGAAGAGCACACGTCTGAACTCCAGTCAC Read1 before filtering: total reads: 3690585 total bases: 446560785 Q20 bases: 440294071(98.5967%) Q30 bases: 423412114(94.8162%) Read1 after filtering: total reads: 3623536 total bases: 359276792 Q20 bases: 356003241(99.0888%) Q30 bases: 343818636(95.6974%) Filtering result: reads passed filter: 3623536 reads failed due to low quality: 27651 reads failed due to too many N: 7 reads failed due to too short: 39391 reads with adapter trimmed: 342799 bases trimmed due to adapters: 10641565 Duplication rate (may be overestimated since this is SE data): 55.4853% JSON report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_002.json HTML report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_002.html /userapps/hb-molgen/fastp/fastp -i /scratch/hb-molgen/NGSdata/20250702_024/Anne/DS024_002_S2_L001_R1_001.fastq.gz -o /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_002.trimmed_tail.fastq.gz --trim_tail1 20 --html /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_002.html --json /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_002.json fastp v0.26.0, time used: 9 seconds Detecting adapter sequence for read1... >Nextera_LMP_Read1_External_Adapter | >Illumina Multiplexing Index Sequencing Primer GATCGGAAGAGCACACGTCTGAACTCCAGTCAC Read1 before filtering: total reads: 3775865 total bases: 456879665 Q20 bases: 449769358(98.4437%) Q30 bases: 431173318(94.3735%) Read1 after filtering: total reads: 3605455 total bases: 355483146 Q20 bases: 352266445(99.0951%) Q30 bases: 340240792(95.7122%) Filtering result: reads passed filter: 3605455 reads failed due to low quality: 31526 reads failed due to too many N: 13 reads failed due to too short: 138871 reads with adapter trimmed: 501144 bases trimmed due to adapters: 22606671 Duplication rate (may be overestimated since this is SE data): 52.2173% JSON report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_003.json HTML report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_003.html /userapps/hb-molgen/fastp/fastp -i /scratch/hb-molgen/NGSdata/20250702_024/Anne/DS024_003_S3_L001_R1_001.fastq.gz -o /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_003.trimmed_tail.fastq.gz --trim_tail1 20 --html /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_003.html --json /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_003.json fastp v0.26.0, time used: 10 seconds Detecting adapter sequence for read1... >Nextera_LMP_Read1_External_Adapter | >Illumina Multiplexing Index Sequencing Primer GATCGGAAGAGCACACGTCTGAACTCCAGTCAC Read1 before filtering: total reads: 3737381 total bases: 452223101 Q20 bases: 441845260(97.7052%) Q30 bases: 422233408(93.3684%) Read1 after filtering: total reads: 3603946 total bases: 353806874 Q20 bases: 350445509(99.0499%) Q30 bases: 338112366(95.5641%) Filtering result: reads passed filter: 3603946 reads failed due to low quality: 88521 reads failed due to too many N: 6 reads failed due to too short: 44908 reads with adapter trimmed: 459510 bases trimmed due to adapters: 14690073 Duplication rate (may be overestimated since this is SE data): 53.4345% JSON report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_004.json HTML report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_004.html /userapps/hb-molgen/fastp/fastp -i /scratch/hb-molgen/NGSdata/20250702_024/Anne/DS024_004_S4_L001_R1_001.fastq.gz -o /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_004.trimmed_tail.fastq.gz --trim_tail1 20 --html /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_004.html --json /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_004.json fastp v0.26.0, time used: 9 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Read1 before filtering: total reads: 3671938 total bases: 444304498 Q20 bases: 434935858(97.8914%) Q30 bases: 417154806(93.8894%) Read1 after filtering: total reads: 3552936 total bases: 346760019 Q20 bases: 343885518(99.171%) Q30 bases: 332910132(96.0059%) Filtering result: reads passed filter: 3552936 reads failed due to low quality: 80937 reads failed due to too many N: 10 reads failed due to too short: 38055 reads with adapter trimmed: 517697 bases trimmed due to adapters: 13932574 Duplication rate (may be overestimated since this is SE data): 53.1501% JSON report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_005.json HTML report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_005.html /userapps/hb-molgen/fastp/fastp -i /scratch/hb-molgen/NGSdata/20250702_024/Anne/DS024_005_S5_L001_R1_001.fastq.gz -o /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_005.trimmed_tail.fastq.gz --trim_tail1 20 --html /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_005.html --json /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_005.json fastp v0.26.0, time used: 8 seconds Detecting adapter sequence for read1... >Nextera_LMP_Read1_External_Adapter | >Illumina Multiplexing Index Sequencing Primer GATCGGAAGAGCACACGTCTGAACTCCAGTCAC Read1 before filtering: total reads: 4742815 total bases: 573880615 Q20 bases: 561673721(97.8729%) Q30 bases: 537156665(93.6008%) Read1 after filtering: total reads: 4595263 total bases: 451087351 Q20 bases: 446795938(99.0487%) Q30 bases: 431043404(95.5565%) Filtering result: reads passed filter: 4595263 reads failed due to low quality: 96406 reads failed due to too many N: 10 reads failed due to too short: 51136 reads with adapter trimmed: 576400 bases trimmed due to adapters: 18148780 Duplication rate (may be overestimated since this is SE data): 56.6417% JSON report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_006.json HTML report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_006.html /userapps/hb-molgen/fastp/fastp -i /scratch/hb-molgen/NGSdata/20250702_024/Anne/DS024_006_S6_L001_R1_001.fastq.gz -o /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_006.trimmed_tail.fastq.gz --trim_tail1 20 --html /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_006.html --json /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_006.json fastp v0.26.0, time used: 12 seconds Detecting adapter sequence for read1... >Nextera_LMP_Read1_External_Adapter | >Illumina Multiplexing Index Sequencing Primer GATCGGAAGAGCACACGTCTGAACTCCAGTCAC Read1 before filtering: total reads: 4736170 total bases: 573076570 Q20 bases: 561039743(97.8996%) Q30 bases: 529824519(92.4527%) Read1 after filtering: total reads: 4439956 total bases: 399270681 Q20 bases: 394895512(98.9042%) Q30 bases: 379433010(95.0315%) Filtering result: reads passed filter: 4439956 reads failed due to low quality: 17596 reads failed due to too many N: 8 reads failed due to too short: 278610 reads with adapter trimmed: 1753036 bases trimmed due to adapters: 77142430 Duplication rate (may be overestimated since this is SE data): 32.7058% JSON report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_007.json HTML report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_007.html /userapps/hb-molgen/fastp/fastp -i /scratch/hb-molgen/NGSdata/20250702_024/Anne/DS024_007_S7_L001_R1_001.fastq.gz -o /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_007.trimmed_tail.fastq.gz --trim_tail1 20 --html /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_007.html --json /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_007.json fastp v0.26.0, time used: 10 seconds Detecting adapter sequence for read1... >Nextera_LMP_Read1_External_Adapter | >Illumina Multiplexing Index Sequencing Primer GATCGGAAGAGCACACGTCTGAACTCCAGTCAC Read1 before filtering: total reads: 5401943 total bases: 653635103 Q20 bases: 638839028(97.7363%) Q30 bases: 599876869(91.7755%) Read1 after filtering: total reads: 4746920 total bases: 423026192 Q20 bases: 418513772(98.9333%) Q30 bases: 402446713(95.1352%) Filtering result: reads passed filter: 4746920 reads failed due to low quality: 17283 reads failed due to too many N: 9 reads failed due to too short: 637731 reads with adapter trimmed: 2218751 bases trimmed due to adapters: 120452567 Duplication rate (may be overestimated since this is SE data): 50.8226% JSON report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_008.json HTML report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_008.html /userapps/hb-molgen/fastp/fastp -i /scratch/hb-molgen/NGSdata/20250702_024/Anne/DS024_008_S8_L001_R1_001.fastq.gz -o /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_008.trimmed_tail.fastq.gz --trim_tail1 20 --html /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_008.html --json /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_008.json fastp v0.26.0, time used: 11 seconds Detecting adapter sequence for read1... >Nextera_LMP_Read1_External_Adapter | >Illumina Multiplexing Index Sequencing Primer GATCGGAAGAGCACACGTCTGAACTCCAGTCAC Read1 before filtering: total reads: 6538007 total bases: 791098847 Q20 bases: 764768655(96.6717%) Q30 bases: 710297424(89.7862%) Read1 after filtering: total reads: 5740385 total bases: 497833627 Q20 bases: 492121808(98.8527%) Q30 bases: 472297503(94.8706%) Filtering result: reads passed filter: 5740385 reads failed due to low quality: 65638 reads failed due to too many N: 23 reads failed due to too short: 731961 reads with adapter trimmed: 3131749 bases trimmed due to adapters: 155478535 Duplication rate (may be overestimated since this is SE data): 54.4448% JSON report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_009.json HTML report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_009.html /userapps/hb-molgen/fastp/fastp -i /scratch/hb-molgen/NGSdata/20250702_024/Anne/DS024_009_S9_L001_R1_001.fastq.gz -o /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_009.trimmed_tail.fastq.gz --trim_tail1 20 --html /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_009.html --json /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_009.json fastp v0.26.0, time used: 13 seconds Detecting adapter sequence for read1... >Nextera_LMP_Read1_External_Adapter | >Illumina Multiplexing Index Sequencing Primer GATCGGAAGAGCACACGTCTGAACTCCAGTCAC Read1 before filtering: total reads: 6001379 total bases: 726166859 Q20 bases: 700767054(96.5022%) Q30 bases: 648261652(89.2717%) Read1 after filtering: total reads: 5179501 total bases: 433129232 Q20 bases: 428295145(98.8839%) Q30 bases: 411082595(94.9099%) Filtering result: reads passed filter: 5179501 reads failed due to low quality: 50374 reads failed due to too many N: 5 reads failed due to too short: 771499 reads with adapter trimmed: 3450366 bases trimmed due to adapters: 167494734 Duplication rate (may be overestimated since this is SE data): 58.5457% JSON report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_010.json HTML report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_010.html /userapps/hb-molgen/fastp/fastp -i /scratch/hb-molgen/NGSdata/20250702_024/Anne/DS024_010_S10_L001_R1_001.fastq.gz -o /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_010.trimmed_tail.fastq.gz --trim_tail1 20 --html /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_010.html --json /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_010.json fastp v0.26.0, time used: 12 seconds Detecting adapter sequence for read1... >Nextera_LMP_Read1_External_Adapter | >Illumina Multiplexing Index Sequencing Primer GATCGGAAGAGCACACGTCTGAACTCCAGTCAC Read1 before filtering: total reads: 5061209 total bases: 612406289 Q20 bases: 587297080(95.8999%) Q30 bases: 536334113(87.5782%) Read1 after filtering: total reads: 3714908 total bases: 323537550 Q20 bases: 319892060(98.8732%) Q30 bases: 307286925(94.9772%) Filtering result: reads passed filter: 3714908 reads failed due to low quality: 73847 reads failed due to too many N: 23 reads failed due to too short: 1272431 reads with adapter trimmed: 2659384 bases trimmed due to adapters: 179382891 Duplication rate (may be overestimated since this is SE data): 67.088% JSON report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_011.json HTML report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_011.html /userapps/hb-molgen/fastp/fastp -i /scratch/hb-molgen/NGSdata/20250702_024/Anne/DS024_011_S11_L001_R1_001.fastq.gz -o /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_011.trimmed_tail.fastq.gz --trim_tail1 20 --html /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_011.html --json /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_011.json fastp v0.26.0, time used: 9 seconds Detecting adapter sequence for read1... >Nextera_LMP_Read1_External_Adapter | >Illumina Multiplexing Index Sequencing Primer GATCGGAAGAGCACACGTCTGAACTCCAGTCAC Read1 before filtering: total reads: 4191818 total bases: 507209978 Q20 bases: 493446720(97.2865%) Q30 bases: 462168455(91.1197%) Read1 after filtering: total reads: 3662843 total bases: 341523004 Q20 bases: 337700240(98.8807%) Q30 bases: 324428046(94.9945%) Filtering result: reads passed filter: 3662843 reads failed due to low quality: 30303 reads failed due to too many N: 8 reads failed due to too short: 498664 reads with adapter trimmed: 1344793 bases trimmed due to adapters: 78468173 Duplication rate (may be overestimated since this is SE data): 42.0021% JSON report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_012.json HTML report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_012.html /userapps/hb-molgen/fastp/fastp -i /scratch/hb-molgen/NGSdata/20250702_024/Anne/DS024_012_S12_L001_R1_001.fastq.gz -o /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_012.trimmed_tail.fastq.gz --trim_tail1 20 --html /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_012.html --json /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_012.json fastp v0.26.0, time used: 9 seconds Detecting adapter sequence for read1... >Nextera_LMP_Read1_External_Adapter | >Illumina Multiplexing Index Sequencing Primer GATCGGAAGAGCACACGTCTGAACTCCAGTCAC Read1 before filtering: total reads: 3546671 total bases: 429147191 Q20 bases: 418582923(97.5383%) Q30 bases: 400674142(93.3652%) Read1 after filtering: total reads: 3399174 total bases: 336644683 Q20 bases: 333744412(99.1385%) Q30 bases: 322961595(95.9355%) Filtering result: reads passed filter: 3399174 reads failed due to low quality: 101051 reads failed due to too many N: 7 reads failed due to too short: 46439 reads with adapter trimmed: 370684 bases trimmed due to adapters: 11295760 Duplication rate (may be overestimated since this is SE data): 55.067% JSON report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_013.json HTML report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_013.html /userapps/hb-molgen/fastp/fastp -i /scratch/hb-molgen/NGSdata/20250702_024/Anne/DS024_013_S13_L001_R1_001.fastq.gz -o /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_013.trimmed_tail.fastq.gz --trim_tail1 20 --html /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_013.html --json /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_013.json fastp v0.26.0, time used: 9 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Read1 before filtering: total reads: 3898617 total bases: 471732657 Q20 bases: 463375869(98.2285%) Q30 bases: 444545526(94.2368%) Read1 after filtering: total reads: 3811595 total bases: 379453434 Q20 bases: 375842946(99.0485%) Q30 bases: 362644887(95.5703%) Filtering result: reads passed filter: 3811595 reads failed due to low quality: 53995 reads failed due to too many N: 7 reads failed due to too short: 33020 reads with adapter trimmed: 296617 bases trimmed due to adapters: 7077500 Duplication rate (may be overestimated since this is SE data): 58.3249% JSON report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_014.json HTML report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_014.html /userapps/hb-molgen/fastp/fastp -i /scratch/hb-molgen/NGSdata/20250702_024/Anne/DS024_014_S14_L001_R1_001.fastq.gz -o /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_014.trimmed_tail.fastq.gz --trim_tail1 20 --html /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_014.html --json /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_014.json fastp v0.26.0, time used: 9 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Read1 before filtering: total reads: 3815907 total bases: 461724747 Q20 bases: 449236671(97.2953%) Q30 bases: 428954969(92.9027%) Read1 after filtering: total reads: 3669940 total bases: 364774752 Q20 bases: 361314044(99.0513%) Q30 bases: 348727666(95.6008%) Filtering result: reads passed filter: 3669940 reads failed due to low quality: 126072 reads failed due to too many N: 4 reads failed due to too short: 19891 reads with adapter trimmed: 318198 bases trimmed due to adapters: 6821349 Duplication rate (may be overestimated since this is SE data): 59.0293% JSON report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_015.json HTML report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_015.html /userapps/hb-molgen/fastp/fastp -i /scratch/hb-molgen/NGSdata/20250702_024/Anne/DS024_015_S15_L001_R1_001.fastq.gz -o /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_015.trimmed_tail.fastq.gz --trim_tail1 20 --html /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_015.html --json /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_015.json fastp v0.26.0, time used: 9 seconds Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Read1 before filtering: total reads: 3750192 total bases: 453773232 Q20 bases: 443414334(97.7172%) Q30 bases: 423727921(93.3788%) Read1 after filtering: total reads: 3635353 total bases: 357592576 Q20 bases: 354204785(99.0526%) Q30 bases: 341557663(95.5159%) Filtering result: reads passed filter: 3635353 reads failed due to low quality: 86078 reads failed due to too many N: 12 reads failed due to too short: 28749 reads with adapter trimmed: 453581 bases trimmed due to adapters: 10898893 Duplication rate (may be overestimated since this is SE data): 61.5875% JSON report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_016.json HTML report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_016.html /userapps/hb-molgen/fastp/fastp -i /scratch/hb-molgen/NGSdata/20250702_024/Anne/DS024_016_S16_L001_R1_001.fastq.gz -o /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_016.trimmed_tail.fastq.gz --trim_tail1 20 --html /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_016.html --json /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_016.json fastp v0.26.0, time used: 9 seconds Detecting adapter sequence for read1... >Nextera_LMP_Read1_External_Adapter | >Illumina Multiplexing Index Sequencing Primer GATCGGAAGAGCACACGTCTGAACTCCAGTCAC Read1 before filtering: total reads: 5164313 total bases: 624881873 Q20 bases: 612915927(98.0851%) Q30 bases: 584250199(93.4977%) Read1 after filtering: total reads: 5081346 total bases: 475849488 Q20 bases: 470530772(98.8823%) Q30 bases: 452517131(95.0967%) Filtering result: reads passed filter: 5081346 reads failed due to low quality: 22621 reads failed due to too many N: 20 reads failed due to too short: 60326 reads with adapter trimmed: 1413625 bases trimmed due to adapters: 43440156 Duplication rate (may be overestimated since this is SE data): 8.44349% JSON report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_017.json HTML report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_017.html /userapps/hb-molgen/fastp/fastp -i /scratch/hb-molgen/NGSdata/20250702_024/Anne/DS024_017_S17_L001_R1_001.fastq.gz -o /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_017.trimmed_tail.fastq.gz --trim_tail1 20 --html /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_017.html --json /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_017.json fastp v0.26.0, time used: 12 seconds Detecting adapter sequence for read1... >Nextera_LMP_Read1_External_Adapter | >Illumina Multiplexing Index Sequencing Primer GATCGGAAGAGCACACGTCTGAACTCCAGTCAC Read1 before filtering: total reads: 5281472 total bases: 639058112 Q20 bases: 624857093(97.7778%) Q30 bases: 592362033(92.693%) Read1 after filtering: total reads: 4952934 total bases: 468217427 Q20 bases: 463739895(99.0437%) Q30 bases: 447169458(95.5047%) Filtering result: reads passed filter: 4952934 reads failed due to low quality: 46359 reads failed due to too many N: 8 reads failed due to too short: 282171 reads with adapter trimmed: 1356772 bases trimmed due to adapters: 60362784 Duplication rate (may be overestimated since this is SE data): 57.5837% JSON report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_018.json HTML report: /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_018.html /userapps/hb-molgen/fastp/fastp -i /scratch/hb-molgen/NGSdata/20250702_024/Anne/DS024_018_S18_L001_R1_001.fastq.gz -o /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_018.trimmed_tail.fastq.gz --trim_tail1 20 --html /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_018.html --json /scratch/p127804/PROJECTS/Douwe/LisaFries_2025_024/fq_trimmed/DS024_018.json fastp v0.26.0, time used: 12 seconds ############################################################################### Hábrók Cluster Job 18111497 for user p127804 Finished at: Thu Jul 3 12:07:51 CEST 2025 Job details: ============ Job ID : 18111497 Name : fastp User : p127804 Partition : regularmedium Nodes : node3 Number of Nodes : 1 Cores : 4 Number of Tasks : 1 State : COMPLETED Submit : 2025-07-03T12:01:39 Start : 2025-07-03T12:01:40 End : 2025-07-03T12:07:47 Reserved walltime : 1-00:00:00 Used walltime : 00:06:07 Used CPU time : 00:21:23 (Efficiency: 87.40%) % User (Computation) : 98.31% % System (I/O) : 1.69% Total memory reserved : 8G Maximum memory used : 1.23G Hints and tips : 1) You requested much more CPU memory than your program used. Please reduce the requested amount of memory. *) For more information on these issues see: https://wiki.hpc.rug.nl/habrok/additional_information/job_hints Acknowledgements: ================= Please see this page for information about acknowledging Hábrók in your publications: https://wiki.hpc.rug.nl/habrok/introduction/scientific_output ################################################################################