FastQCFastQC Report
Tue 4 Mar 2025
DS022_12_S12_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDS022_12_S12_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3865713
Sequences flagged as poor quality0
Sequence length121
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTGGGGG816402.1119001850370167TruSeq Adapter, Index 7 (97% over 35bp)
CCCGACTTTCGTCCCTGCTCGGACCGTCGTCCTCGCAGTCAAGCCCGCTT300590.7775797116857873No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTGGGG273020.7062603974997627TruSeq Adapter, Index 7 (97% over 35bp)
CCCGCTTCGGGTCCAGGACACGCGACTCAAACGCCTTTTAAGACTCGCCT235360.6088398181654975No Hit
CGCGTCTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAACC194020.5018996495601199No Hit
CCGACTTTCGTCCCTGCTCGGACCGTCGTCCTCGCAGTCAAGCCCGCTTG158940.4111531300952761No Hit
GCCGCGTCTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAA145280.37581682861609234No Hit
CCGCGTCTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAAC133480.3452920586706773No Hit
GGGGACTACAGGAACTCGAAGGTTCCACGGAATGCTTATCTTGGAGCAGG128930.33352191432731815No Hit
CTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAACCACTAG102930.26626394665098No Hit
CCCCGCTACATTGTCGGCGCGGAACCACTAGACCAGTGAGCTGTTACGCA99420.25718412101467436No Hit
CTCCCGATGAGGCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGT95630.2473799788033928No Hit
GGCGATTTTTCTGCCCCCATCGATGTCGCAGACAACTGATGGCGAGCATA90660.2345233595975697No Hit
GGAGACGCGATCGGCAGACCATGCGCGTTCAGGTACGGGGCCCTCACCCT88910.22999638100397002No Hit
CTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCACGT88310.228444274057593No Hit
CTCGCCTTCGCTACGCATACCCCACACGGGTTATGCTCGCCACATGCCAC75020.1940651051953417No Hit
CTCCTACCCAGTACAAAATACTGCCGCGTCTTCGGTGGTGTGCTTGAGCC72440.1873910453259205No Hit
CGCCGACTCACTGCCGGGATACGCGTCACCGGTATTCGGAGTTTGGTTGC68110.17619000686289957No Hit
CTTCGCTACGCATACCCCACACGGGTTATGCTCGCCACATGCCACTGACT67780.17533634804239218No Hit
GTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCACGTCCGGC66270.1714302122273433No Hit
CACGTGTTCTGCATGGGGACTACAGGAACTCGAAGGTTCCACGGAATGCT64240.1661789170587677No Hit
CTGGGCTGTTTCCCTTTCGACGACGAAGCTTATCCCCCGCCGACTCACTG62830.16253146573478164No Hit
TCCCGCTTCGGGTCCAGGACACGCGACTCAAACGCCTTTTAAGACTCGCC60960.15769406575190656No Hit
GCTAGATCATCCCGCTTCGGGTCCAGGACACGCGACTCAAACGCCTTTTA56780.1468810540254799No Hit
GTCGGTTTGGGGTACGGGCGGCTTCGAGCCTGACGCCGAAGCTTTTCTCG55740.14419073531842638No Hit
GTTCTGCATGGGGACTACAGGAACTCGAAGGTTCCACGGAATGCTTATCT54930.14209539094081738No Hit
GGCCTTTCACCCCTAGCCCCAAGTCATCCCCCCGGTTTTCAACCCAGGTG54660.14139694281494772No Hit
TTCCCTTTCGACGACGAAGCTTATCCCCCGCCGACTCACTGCCGGGATAC53530.13847380806593765No Hit
CCACGTGTTCTGCATGGGGACTACAGGAACTCGAAGGTTCCACGGAATGC52840.13668888507760404No Hit
GCGTCTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAACCA52630.1361456476463721No Hit
GCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCAC51910.13428311931071965No Hit
GTGGGTTCGGTCCTCCACGCGGTCTTACCCGCGCTTCAACCTGCTCAGGG51640.13358467118484998No Hit
CTGCCGCGTCTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGG50930.13174801129830382No Hit
CTCATTTTTCGATAGGCACGCCGTCACCCCACAAAAGGGCTCCGACGGAT50460.13053219419030848No Hit
CTTTCGACGACGAAGCTTATCCCCCGCCGACTCACTGCCGGGATACGCGT50320.13017003590282053No Hit
CGGGGTTCCACGGGACCCGTGCTACTTGGGAGACGCGATCGGCAGACCAT50290.13009243055550165No Hit
CTCGGTTTTGGCGGCTGAAAGCCGGTACGGGAATATCGACCCGTTCATCC48870.12641911078240933No Hit
CCGCCATCTGCTTGGCCTTGCTCCCGATGAGGCTTGCCATGCGACCTACT48560.12561718886011455No Hit
CGACTTTCGTCCCTGCTCGGACCGTCGTCCTCGCAGTCAAGCCCGCTTGT48200.12468592469228833No Hit
CCTGGCTGTCTATGCGACTCCACATCCTTTCCCACTTAGCACACGCTTCG48140.12453071399765063No Hit
CGTGCGCGAACGCACGGCACGGATGGCCATCCATCTCGACCTGACGTTGC46430.12010720920047609No Hit
GTCGAAGCCACGGTGGGCCGTTACCCCGCCGTCAAGCTGATAGGACGCGA46020.11904660278711844No Hit
CTCTCCTACCCAGTACAAAATACTGCCGCGTCTTCGGTGGTGTGCTTGAG45650.11808947017018594No Hit
GTGCGCGAACGCACGGCACGGATGGCCATCCATCTCGACCTGACGTTGCC45310.1172099429005723No Hit
TGCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCA45230.11700299530772201No Hit
CGCCTTCGCTACGCATACCCCACACGGGTTATGCTCGCCACATGCCACTG45140.11677017926576545No Hit
GCCTTCGCTACGCATACCCCACACGGGTTATGCTCGCCACATGCCACTGA45080.11661496857112774No Hit
CTAGATCATCCCGCTTCGGGTCCAGGACACGCGACTCAAACGCCTTTTAA44940.11625281028363979No Hit
CTGACACATGGTTTTCTGCAACTGCCGGCCGGCCCGTCGGAGCCGGCGCA44810.11591652044525809No Hit
GGCGGATTCGCACGGGGTTCCACGGGACCCGTGCTACTTGGGAGACGCGA44330.11467483488815647No Hit
CCGCTACATTGTCGGCGCGGAACCACTAGACCAGTGAGCTGTTACGCACT43960.11371770227122396No Hit
CGGGCGGCTTCGAGCCTGACGCCGAAGCTTTTCTCGGCAGCCGGGATCAC43880.1135107546783737No Hit
GGAGGCGCGGGGAATTGAACCCCGGTCCGATGACCGAGCCCACAGTCTTC43570.11270883275607889No Hit
GTTGCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTC43550.11265709585786632No Hit
CCCCCGCCGACTCACTGCCGGGATACGCGTCACCGGTATTCGGAGTTTGG43280.11195864773199667No Hit
CCTCACCCTCTGCGGCCCGGTATTCAACCCGGTTCCCCTGACACATGGTT42790.11069109372578874No Hit
CCTGACACATGGTTTTCTGCAACTGCCGGCCGGCCCGTCGGAGCCGGCGC42710.11048414613293847No Hit
CTTCGGGTCCAGGACACGCGACTCAAACGCCTTTTAAGACTCGCCTTCGC42620.11025133009098192No Hit
CACGGTTTCAGAGACTGTTTCACTCCCCTCCCGGGGTACTTTTCACCTTT42620.11025133009098192No Hit
CCCTCACCCTCTGCGGCCCGGTATTCAACCCGGTTCCCCTGACACATGGT42390.10965635576153739No Hit
GTCCAGGACACGCGACTCAAACGCCTTTTAAGACTCGCCTTCGCTACGCA42310.10944940816868712No Hit
TGCCGCGTCTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGA42250.1092941974740494No Hit
CCGCTTCGGGTCCAGGACACGCGACTCAAACGCCTTTTAAGACTCGCCTT41740.10797490656962894No Hit
CCACGTTAGTACGCGATTCACGCGGCCACGCGCTGAACCTTGCCAGCCTT41660.10776795897677867No Hit
CCCTGACACATGGTTTTCTGCAACTGCCGGCCGGCCCGTCGGAGCCGGCG41240.10668148411431475No Hit
CAGGAATATTCAGGCTTACCCAACGGTCTGGGCGGATTCGCACGGGGTTC40040.10357727022156067No Hit
GTCAGACAGGAATATTCAGGCTTACCCAACGGTCTGGGCGGATTCGCACG39940.10331858573049783No Hit
CGACGGTCTGGGCTGTTTCCCTTTCGACGACGAAGCTTATCCCCCGCCGA39900.1032151119340727No Hit
CTCAGGGCTAGATCATCCCGCTTCGGGTCCAGGACACGCGACTCAAACGC39440.10202516327518364No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)