FastQCFastQC Report
Tue 4 Mar 2025
DS022_10_S10_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDS022_10_S10_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6822809
Sequences flagged as poor quality0
Sequence length121
%GC60

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTGGGGG1906052.793644084130158TruSeq Adapter, Index 7 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTGGGG443060.649380629004857TruSeq Adapter, Index 7 (97% over 35bp)
CGCGTCTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAACC413070.6054251262200071No Hit
GCCGCGTCTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAA412960.6052639023018231No Hit
CCGCGTCTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAAC372030.5452739480175981No Hit
CCCGCTTCGGGTCCAGGACACGCGACTCAAACGCCTTTTAAGACTCGCCT269980.3957021221024947No Hit
CTCCTACCCAGTACAAAATACTGCCGCGTCTTCGGTGGTGTGCTTGAGCC258620.379052088370054No Hit
CTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAACCACTAG246310.3610096662532983No Hit
CCCCGCTACATTGTCGGCGCGGAACCACTAGACCAGTGAGCTGTTACGCA235390.345004528193593No Hit
CCCGACTTTCGTCCCTGCTCGGACCGTCGTCCTCGCAGTCAAGCCCGCTT198830.29141956047721695No Hit
CTGGGCTGTTTCCCTTTCGACGACGAAGCTTATCCCCCGCCGACTCACTG178900.26220871784627126No Hit
CCTGGCTGTCTATGCGACTCCACATCCTTTCCCACTTAGCACACGCTTCG178310.26134397137601245No Hit
CTGCCGCGTCTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGG173900.25488035792882374No Hit
CTCTCCTACCCAGTACAAAATACTGCCGCGTCTTCGGTGGTGTGCTTGAG170210.2494720283097475No Hit
GCGTCTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAACCA167440.24541211691548157No Hit
GTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAACCACTAGACCAGTGA156750.22974408341197883No Hit
CTGCATTCTCACTCCCATGAAGTCCACGGCGCGTTCACACGGCCGCTTCG155540.22797062031195658No Hit
GGAGGCGCGGGGAATTGAACCCCGGTCCGATGACCGAGCCCACAGTCTTC153790.22540569434084992No Hit
CTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCACGT144220.21137921345885544No Hit
GGAGACGCGATCGGCAGACCATGCGCGTTCAGGTACGGGGCCCTCACCCT141580.20750983942244317No Hit
CTCGGTTTTGGCGGCTGAAAGCCGGTACGGGAATATCGACCCGTTCATCC139980.20516476424886No Hit
CCACGTTAGTACGCGATTCACGCGGCCACGCGCTGAACCTTGCCAGCCTT136790.20048927062152844No Hit
GTGGGTTCGGTCCTCCACGCGGTCTTACCCGCGCTTCAACCTGCTCAGGG134490.19711822505950263No Hit
GTCGGTTTGGGGTACGGGCGGCTTCGAGCCTGACGCCGAAGCTTTTCTCG127580.18699043165359017No Hit
GGCCTTTCACCCCTAGCCCCAAGTCATCCCCCCGGTTTTCAACCCAGGTG126650.18562735670894495No Hit
GCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCAC118200.1732424284484587No Hit
CCGCTACATTGTCGGCGCGGAACCACTAGACCAGTGAGCTGTTACGCACT116090.17014986056329587No Hit
CTCCCGATGAGGCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGT113170.1658700983715065No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTATCTCAGTCCCAATGT108750.15939182820448292No Hit
CACGTTAGTACGCGATTCACGCGGCCACGCGCTGAACCTTGCCAGCCTTG105890.15520000633170297No Hit
GTTGCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTC103210.1512720054159511No Hit
GGCGGATTCGCACGGGGTTCCACGGGACCCGTGCTACTTGGGAGACGCGA102270.14989427375147096No Hit
TGCCGCGTCTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGA100730.14763713889689717No Hit
CTGGCTGTCTATGCGACTCCACATCCTTTCCCACTTAGCACACGCTTCGG100700.14759316873739248No Hit
CCCGCAACACCCCGCGCGCAACCCCCGACGGGTATCACACGCGCAAGGTT100550.14737331793986905No Hit
GGGGACTACAGGAACTCGAAGGTTCCACGGAATGCTTATCTTGGAGCAGG99550.14590764595637956No Hit
GGCGATTTTTCTGCCCCCATCGATGTCGCAGACAACTGATGGCGAGCATA98880.1449256457274416No Hit
GCTAGATCATCCCGCTTCGGGTCCAGGACACGCGACTCAAACGCCTTTTA98470.1443247202142109No Hit
CTCGCCTTCGCTACGCATACCCCACACGGGTTATGCTCGCCACATGCCAC94410.13837409196124353No Hit
CGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAACCACTAGACC93640.1372455245339566No Hit
GGCGGCTGAAAGCCGGTACGGGAATATCGACCCGTTCATCCATTCGACTA93490.1370256737364332No Hit
CCGACTTTCGTCCCTGCTCGGACCGTCGTCCTCGCAGTCAAGCCCGCTTG92340.13534015095542026No Hit
GTCAGACAGGAATATTCAGGCTTACCCAACGGTCTGGGCGGATTCGCACG89690.1314561201991731No Hit
TGCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCA89510.13119229924214498No Hit
CGACGGTCTGGGCTGTTTCCCTTTCGACGACGAAGCTTATCCCCCGCCGA88660.12994647805617893No Hit
CTTTCGACGACGAAGCTTATCCCCCGCCGACTCACTGCCGGGATACGCGT88540.1297705974181602No Hit
CCCGCTACATTGTCGGCGCGGAACCACTAGACCAGTGAGCTGTTACGCAC86810.12723498488672333No Hit
GTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCACGTCCGGC82650.12113778943540703No Hit
GCCTTTCACCCCTAGCCCCAAGTCATCCCCCCGGTTTTCAACCCAGGTGG81150.1189392814601728No Hit
CTTCGCTACGCATACCCCACACGGGTTATGCTCGCCACATGCCACTGACT80590.11811850514941867No Hit
CGGGCGGCTTCGAGCCTGACGCCGAAGCTTTTCTCGGCAGCCGGGATCAC79310.1162424450105521No Hit
CCTTGGTCATCCAGCGGACGGGATCTTCACCCGTCTCTCGCTACTCATGT78130.1145129520700345No Hit
GGGAGACGCGATCGGCAGACCATGCGCGTTCAGGTACGGGGCCCTCACCC77480.11356026528076632No Hit
GGCTAGATCATCCCGCTTCGGGTCCAGGACACGCGACTCAAACGCCTTTT76510.11213856345678151No Hit
GGGCTGTTTCCCTTTCGACGACGAAGCTTATCCCCCGCCGACTCACTGCC76470.11207993657744192No Hit
CGCCGACTCACTGCCGGGATACGCGTCACCGGTATTCGGAGTTTGGTTGC76400.11197733953859768No Hit
GCTCTCCTACCCAGTACAAAATACTGCCGCGTCTTCGGTGGTGTGCTTGA76150.1116109215427253No Hit
CCCAGTACAAAATACTGCCGCGTCTTCGGTGGTGTGCTTGAGCCCCGCTA74710.10950035388650041No Hit
GCACGGTTTCAGAGACTGTTTCACTCCCCTCCCGGGGTACTTTTCACCTT73980.10843041333855308No Hit
GCCTTCGCTACGCATACCCCACACGGGTTATGCTCGCCACATGCCACTGA73860.10825453270053434No Hit
CCGCCATCTGCTTGGCCTTGCTCCCGATGAGGCTTGCCATGCGACCTACT72030.10557235297074855No Hit
CCTTAGACGACGGTCTGGGCTGTTTCCCTTTCGACGACGAAGCTTATCCC71800.10523524841454597No Hit
CGCACGGTTTCAGAGACTGTTTCACTCCCCTCCCGGGGTACTTTTCACCT70950.10398942722857989No Hit
CCCTGGTCTGTGCCACCCCCAAAAGCTCCCACAGCAAGTGTGTTCACCAT70850.10384286003023095No Hit
CACGGTTTCAGAGACTGTTTCACTCCCCTCCCGGGGTACTTTTCACCTTT70550.10340315843518409No Hit
CCTGACACATGGTTTTCTGCAACTGCCGGCCGGCCCGTCGGAGCCGGCGC70430.10322727779716535No Hit
CTCATTTTTCGATAGGCACGCCGTCACCCCACAAAAGGGCTCCGACGGAT70240.10294880012030236No Hit
CCTCGGTTTTGGCGGCTGAAAGCCGGTACGGGAATATCGACCCGTTCATC69900.10245047164591592No Hit
CACGGAATTTGATTGGCCTTTCACCCCTAGCCCCAAGTCATCCCCCCGGT69850.10237718804674147No Hit
CCCAAATGCATTTCGGAGAGAACCAGCTATCACGGAATTTGATTGGCCTT69780.10227459100789718No Hit
CAGGGCTAGATCATCCCGCTTCGGGTCCAGGACACGCGACTCAAACGCCT69720.10218665068888783No Hit
CCCTCACCCTCTGCGGCCCGGTATTCAACCCGGTTCCCCTGACACATGGT68940.101043426541766No Hit
CCGGGACGTAATGACCCGACGCTGCACCCAAATGCATTTCGGAGAGAACC68830.10088220262358216No Hit
GCTGTCTATGCGACTCCACATCCTTTCCCACTTAGCACACGCTTCGGGAC68750.100764948864903No Hit
TCCCGCTTCGGGTCCAGGACACGCGACTCAAACGCCTTTTAAGACTCGCC68350.10017868007150721No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)