FastQCFastQC Report
Tue 4 Mar 2025
DS022_09_S9_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDS022_09_S9_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2677137
Sequences flagged as poor quality0
Sequence length121
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTGGGGG212030.7920028000061259TruSeq Adapter, Index 7 (97% over 35bp)
CTCCCGATGAGGCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGT132810.4960896659379031No Hit
CCCGCTTCGGGTCCAGGACACGCGACTCAAACGCCTTTTAAGACTCGCCT116760.4361375603863381No Hit
CCCGACTTTCGTCCCTGCTCGGACCGTCGTCCTCGCAGTCAAGCCCGCTT111270.4156305784873916No Hit
GGCGATTTTTCTGCCCCCATCGATGTCGCAGACAACTGATGGCGAGCATA105750.3950115365780683No Hit
GGGGACTACAGGAACTCGAAGGTTCCACGGAATGCTTATCTTGGAGCAGG89210.33322911752368295No Hit
CCACGTTAGTACGCGATTCACGCGGCCACGCGCTGAACCTTGCCAGCCTT86410.3227701832218523No Hit
GGAGGCGCGGGGAATTGAACCCCGGTCCGATGACCGAGCCCACAGTCTTC71320.26640399800234354No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTGGGG69170.2583730305920093TruSeq Adapter, Index 7 (97% over 35bp)
CACGTTAGTACGCGATTCACGCGGCCACGCGCTGAACCTTGCCAGCCTTG63320.2365213285685417No Hit
CGCGTCTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAACC62020.2316653947855489No Hit
CTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCACGT61080.22815418112707717No Hit
CCGCGTCTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAAC59570.22251382727144706No Hit
GTTGCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTC57370.21429609317715156No Hit
GCCGCGTCTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAA57340.21418403316677478No Hit
GGAGACGCGATCGGCAGACCATGCGCGTTCAGGTACGGGGCCCTCACCCT54340.2029780321290991No Hit
CCCGATGAGGCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGTCT53900.20133448531024No Hit
GCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCAC52690.19681473155837748No Hit
CGCCGTTTCACCCTTACCCGTCGAGGACGGGCGGTCTGTTCTCTGTTGCG49820.18609432389900107No Hit
CCGCCATCTGCTTGGCCTTGCTCCCGATGAGGCTTGCCATGCGACCTACT47950.17910924991884986No Hit
GTCGGTTTGGGGTACGGGCGGCTTCGAGCCTGACGCCGAAGCTTTTCTCG45210.16887443563777274No Hit
CTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAACCACTAG42970.16050728819630822No Hit
CCCCGCTACATTGTCGGCGCGGAACCACTAGACCAGTGAGCTGTTACGCA42850.1600590481548012No Hit
CTCGGTTTTGGCGGCTGAAAGCCGGTACGGGAATATCGACCCGTTCATCC42670.15938668809254067No Hit
TGCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCA42430.1584902080095266No Hit
CCGTGCGCGAACGCACGGCACGGATGGCCATCCATCTCGACCTGACGTTG42040.15703342787462876No Hit
CGTGCGCGAACGCACGGCACGGATGGCCATCCATCTCGACCTGACGTTGC41490.1549789943510549No Hit
GTTCTGCATGGGGACTACAGGAACTCGAAGGTTCCACGGAATGCTTATCT38360.1432873999350799No Hit
GTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCACGTCCGGC38290.14302592657753413No Hit
CCGACTTTCGTCCCTGCTCGGACCGTCGTCCTCGCAGTCAAGCCCGCTTG37250.1391411795511399No Hit
CTGGGCTGTTTCCCTTTCGACGACGAAGCTTATCCCCCGCCGACTCACTG35790.13368759237947106No Hit
CTCCTACCCAGTACAAAATACTGCCGCGTCTTCGGTGGTGTGCTTGAGCC35630.13308993899079502No Hit
CTTCGCTACGCATACCCCACACGGGTTATGCTCGCCACATGCCACTGACT35600.13297787898041827No Hit
CTCGCCTTCGCTACGCATACCCCACACGGGTTATGCTCGCCACATGCCAC34230.1278604718398797No Hit
GGCCTTTCACCCCTAGCCCCAAGTCATCCCCCCGGTTTTCAACCCAGGTG32420.12109951788048202No Hit
CCGCCGTTTCACCCTTACCCGTCGAGGACGGGCGGTCTGTTCTCTGTTGC31870.11904508435690814No Hit
CCCGCAACACCCCGCGCGCAACCCCCGACGGGTATCACACGCGCAAGGTT31290.11687859082295751No Hit
CACGTGTTCTGCATGGGGACTACAGGAACTCGAAGGTTCCACGGAATGCT31140.11631829077107374No Hit
TCCCGATGAGGCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGTC31020.1158700507295667No Hit
GGGAGACGCGATCGGCAGACCATGCGCGTTCAGGTACGGGGCCCTCACCC30310.11321796381731679No Hit
CTCATTTTTCGATAGGCACGCCGTCACCCCACAAAAGGGCTCCGACGGAT29650.11075264358902813No Hit
GTCAGACAGGAATATTCAGGCTTACCCAACGGTCTGGGCGGATTCGCACG29460.11004293018997535No Hit
GTGGGTTCGGTCCTCCACGCGGTCTTACCCGCGCTTCAACCTGCTCAGGG29330.10955733681167605No Hit
GTGCGCGAACGCACGGCACGGATGGCCATCCATCTCGACCTGACGTTGCC28980.10824997002394723No Hit
GGCGGATTCGCACGGGGTTCCACGGGACCCGTGCTACTTGGGAGACGCGA28640.10697995657301064No Hit
CCTGGCTGTCTATGCGACTCCACATCCTTTCCCACTTAGCACACGCTTCG28300.10570994312207407No Hit
CTCTCCTACCCAGTACAAAATACTGCCGCGTCTTCGGTGGTGTGCTTGAG28110.10500022972302127No Hit
CTGCCGCGTCTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGG28030.10470140302868325No Hit
CCACGTGTTCTGCATGGGGACTACAGGAACTCGAAGGTTCCACGGAATGC27510.10275902951548614No Hit
TCCCGCTTCGGGTCCAGGACACGCGACTCAAACGCCTTTTAAGACTCGCC27280.10189990276926433No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)