FastQCFastQC Report
Tue 4 Mar 2025
DS022_08_S8_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDS022_08_S8_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7216736
Sequences flagged as poor quality0
Sequence length121
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCGATGAGGCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGT386500.5355606745210023No Hit
CTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCACGT276520.3831649100091787No Hit
GGAGGCGCGGGGAATTGAACCCCGGTCCGATGACCGAGCCCACAGTCTTC232140.3216689650279572No Hit
GGCGATTTTTCTGCCCCCATCGATGTCGCAGACAACTGATGGCGAGCATA227760.31559973927271273No Hit
CCGCCATCTGCTTGGCCTTGCTCCCGATGAGGCTTGCCATGCGACCTACT203790.2823852777765461No Hit
CCCGCTTCGGGTCCAGGACACGCGACTCAAACGCCTTTTAAGACTCGCCT199910.27700888601162627No Hit
CCCGACTTTCGTCCCTGCTCGGACCGTCGTCCTCGCAGTCAAGCCCGCTT196590.27240846831587023No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTGGGGG194790.2699142659507013TruSeq Adapter, Index 7 (97% over 38bp)
GCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCAC187870.26032544352460724No Hit
CGTGCGCGAACGCACGGCACGGATGGCCATCCATCTCGACCTGACGTTGC184360.25546174891252776No Hit
GGGGACTACAGGAACTCGAAGGTTCCACGGAATGCTTATCTTGGAGCAGG179080.2481454219746988No Hit
CCCGATGAGGCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGTCT171140.2371432182083424No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCAAGAGATTCCATCTGGGGG158020.21896325430222197TruSeq Adapter, Index 9 (97% over 35bp)
GTGCGCGAACGCACGGCACGGATGGCCATCCATCTCGACCTGACGTTGCC154940.2146953969218217No Hit
TGCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCA145120.2010881373518444No Hit
CGCCGTTTCACCCTTACCCGTCGAGGACGGGCGGTCTGTTCTCTGTTGCG143130.19833065807035202No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTGGGG142100.1969034200502831TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCAGGAGATTCCATCTGGGGG135780.18814599841257876TruSeq Adapter, Index 9 (97% over 35bp)
CCGTGCGCGAACGCACGGCACGGATGGCCATCCATCTCGACCTGACGTTG130480.18080195811513683No Hit
GTTGCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTC128980.17872345614416268No Hit
GTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCACGTCCGGC125660.1741230384484066No Hit
CCACGTTAGTACGCGATTCACGCGGCCACGCGCTGAACCTTGCCAGCCTT118480.16417394234734373No Hit
GGGGAATTGAACCCCGGTCCGATGACCGAGCCCACAGTCTTCTACGTGCG113540.15732874252293558No Hit
TCCCGATGAGGCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGTC112160.15541652070963938No Hit
CGATGAGGCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGTCTCT100440.13917649197642812No Hit
GTTCTGCATGGGGACTACAGGAACTCGAAGGTTCCACGGAATGCTTATCT98180.13604488234016043No Hit
CCGACTTTCGTCCCTGCTCGGACCGTCGTCCTCGCAGTCAAGCCCGCTTG96790.13411880384705774No Hit
CGCGTCTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAACC96500.1337169601326694No Hit
CACGTTAGTACGCGATTCACGCGGCCACGCGCTGAACCTTGCCAGCCTTG95110.1317908816395667No Hit
CACGTGTTCTGCATGGGGACTACAGGAACTCGAAGGTTCCACGGAATGCT93630.12974009302820555No Hit
GGCGCGGGGAATTGAACCCCGGTCCGATGACCGAGCCCACAGTCTTCTAC91330.12655305667271188No Hit
GCGGGGAATTGAACCCCGGTCCGATGACCGAGCCCACAGTCTTCTACGTG89730.1243359879036728No Hit
CTCCTACCCAGTACAAAATACTGCCGCGTCTTCGGTGGTGTGCTTGAGCC87350.12103809810972715No Hit
GGAGACGCGATCGGCAGACCATGCGCGTTCAGGTACGGGGCCCTCACCCT84660.11731065124178021No Hit
CCACAGTCTTCTACGTGCGTAGTCTGCTGGCCAAGTTGGCGATTTTTCTG83300.115426142788097No Hit
CCGATGAGGCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGTCTC82660.11453931528048136No Hit
CCACGTGTTCTGCATGGGGACTACAGGAACTCGAAGGTTCCACGGAATGC81070.11233610319124879No Hit
CCGCGTCTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAAC78010.10809595917046155No Hit
GCCGCGTCTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAA76760.10636387419464977No Hit
GCCCTTACCGCCATCTGCTTGGCCTTGCTCCCGATGAGGCTTGCCATGCG75570.10471492929767696No Hit
GCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGTCTCTTACACCG75410.10449322242077304No Hit
CGATGACCGAGCCCACAGTCTTCTACGTGCGTAGTCTGCTGGCCAAGTTG74890.10377267507083535No Hit
GCGATTTTTCTGCCCCCATCGATGTCGCAGACAACTGATGGCGAGCATAT72270.10014222496153387No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)