FastQCFastQC Report
Tue 4 Mar 2025
DS022_07_S7_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDS022_07_S7_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8030223
Sequences flagged as poor quality0
Sequence length121
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGATTTTTCTGCCCCCATCGATGTCGCAGACAACTGATGGCGAGCATA610450.7601905949560803No Hit
CTCCCGATGAGGCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGT570890.7109267077639064No Hit
CCCGACTTTCGTCCCTGCTCGGACCGTCGTCCTCGCAGTCAAGCCCGCTT416420.518565922764536No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTGGGGG413760.5152534369219883TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCAAGAGATTCCATCTGGGGG407930.5079933645678333TruSeq Adapter, Index 9 (97% over 35bp)
GGGGACTACAGGAACTCGAAGGTTCCACGGAATGCTTATCTTGGAGCAGG334230.4162150914115336No Hit
CTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCACGT329490.41031239107556544No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCAGGAGATTCCATCTGGGGG295830.3683957469176136TruSeq Adapter, Index 9 (97% over 35bp)
CGCCGTTTCACCCTTACCCGTCGAGGACGGGCGGTCTGTTCTCTGTTGCG280210.348944232308368No Hit
CCCGCTTCGGGTCCAGGACACGCGACTCAAACGCCTTTTAAGACTCGCCT242050.301423758717535No Hit
GCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCAC228530.28458736451030064No Hit
CCGCCATCTGCTTGGCCTTGCTCCCGATGAGGCTTGCCATGCGACCTACT220250.2742763183537991No Hit
GTTGCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTC199870.2488971974999947No Hit
GGAGGCGCGGGGAATTGAACCCCGGTCCGATGACCGAGCCCACAGTCTTC196340.24450130463375672No Hit
CGTGCGCGAACGCACGGCACGGATGGCCATCCATCTCGACCTGACGTTGC194200.24183637241456435No Hit
TGCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCA191500.2384740747548356No Hit
CCCGATGAGGCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGTCT181990.2266313152200132No Hit
CCGACTTTCGTCCCTGCTCGGACCGTCGTCCTCGCAGTCAAGCCCGCTTG178030.2216999453190777No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTGGGG172970.2153987504456601TruSeq Adapter, Index 7 (97% over 38bp)
GCGATTTTTCTGCCCCCATCGATGTCGCAGACAACTGATGGCGAGCATAT170940.2128708007237159No Hit
CCGCCGTTTCACCCTTACCCGTCGAGGACGGGCGGTCTGTTCTCTGTTGC153570.19124001911279426No Hit
GTGCGCGAACGCACGGCACGGATGGCCATCCATCTCGACCTGACGTTGCC148120.1844531590218603No Hit
CGCGTCTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAACC146190.18204973884286899No Hit
GTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCACGTCCGGC142290.17719308666770525No Hit
CCGCGTCTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAAC138430.17238624630972266No Hit
CCGTGCGCGAACGCACGGCACGGATGGCCATCCATCTCGACCTGACGTTG137730.17151453950905224No Hit
CCCCGCTACATTGTCGGCGCGGAACCACTAGACCAGTGAGCTGTTACGCA124530.15507663983926723No Hit
CCACAGTCTTCTACGTGCGTAGTCTGCTGGCCAAGTTGGCGATTTTTCTG117560.14639693069544893No Hit
GGGGAATTGAACCCCGGTCCGATGACCGAGCCCACAGTCTTCTACGTGCG112920.14061875990243358No Hit
CGATGACCGAGCCCACAGTCTTCTACGTGCGTAGTCTGCTGGCCAAGTTG112900.14059385399384303No Hit
GCCGCGTCTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAA112450.1400334710505549No Hit
TGGCGATTTTTCTGCCCCCATCGATGTCGCAGACAACTGATGGCGAGCAT110930.13814062199767055No Hit
CACGTGTTCTGCATGGGGACTACAGGAACTCGAAGGTTCCACGGAATGCT105570.13146583849539423No Hit
CTCCTACCCAGTACAAAATACTGCCGCGTCTTCGGTGGTGTGCTTGAGCC105200.13100507918646842No Hit
CGATGAGGCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGTCTCT103500.12888807695626883No Hit
TCCCGATGAGGCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGTC102710.1279042935669408No Hit
GTGCGTAGTCTGCTGGCCAAGTTGGCGATTTTTCTGCCCCCATCGATGTC102400.12751825198378675No Hit
GTCGGTTTGGGGTACGGGCGGCTTCGAGCCTGACGCCGAAGCTTTTCTCG100390.12501520817043313No Hit
CTCGGTTTTGGCGGCTGAAAGCCGGTACGGGAATATCGACCCGTTCATCC98910.12317217093472996No Hit
CCACGTGTTCTGCATGGGGACTACAGGAACTCGAAGGTTCCACGGAATGC95570.11901288420010254No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCAAGAGATTCCATCTGGGG95460.11887590170285432TruSeq Adapter, Index 9 (97% over 35bp)
GGAGACGCGATCGGCAGACCATGCGCGTTCAGGTACGGGGCCCTCACCCT93930.1169705996956747No Hit
CTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGTCTCTTACACCGC92890.11567549244896437No Hit
GTTCTGCATGGGGACTACAGGAACTCGAAGGTTCCACGGAATGCTTATCT88960.11078148141091475No Hit
CTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAACCACTAG87860.10941165643843266No Hit
GCCGTTTCACCCTTACCCGTCGAGGACGGGCGGTCTGTTCTCTGTTGCGC87480.10893844417521156No Hit
GCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGTCTCTTACACCG84950.10578784673850278No Hit
CCGATGAGGCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGTCTC81870.10195233681555294No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCAGGAGATTCCATCTGGGG81550.10155384227810361TruSeq Adapter, Index 9 (97% over 35bp)
GGCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGTCTCTTACACC80700.10049534116300382No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)