FastQCFastQC Report
Tue 4 Mar 2025
DS022_06_S6_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDS022_06_S6_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7954175
Sequences flagged as poor quality0
Sequence length121
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGACTTTCGTCCCTGCTCGGACCGTCGTCCTCGCAGTCAAGCCCGCTT784310.986035635373876No Hit
CTCCCGATGAGGCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGT434370.5460905750753535No Hit
GGCGATTTTTCTGCCCCCATCGATGTCGCAGACAACTGATGGCGAGCATA427120.5369758648759928No Hit
CCGACTTTCGTCCCTGCTCGGACCGTCGTCCTCGCAGTCAAGCCCGCTTG389910.4901954005286532No Hit
CTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCACGT304380.38266696420433294No Hit
CCCGCTTCGGGTCCAGGACACGCGACTCAAACGCCTTTTAAGACTCGCCT294280.36996922999556836No Hit
GGAGGCGCGGGGAATTGAACCCCGGTCCGATGACCGAGCCCACAGTCTTC250760.3152558247712679No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTGGGGG234440.29473829781215527TruSeq Adapter, Index 7 (97% over 38bp)
GCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCAC231300.290790685394777No Hit
CCGCCATCTGCTTGGCCTTGCTCCCGATGAGGCTTGCCATGCGACCTACT215670.2711406274063621No Hit
GGGGACTACAGGAACTCGAAGGTTCCACGGAATGCTTATCTTGGAGCAGG212830.2675701754110263No Hit
CGCCGTTTCACCCTTACCCGTCGAGGACGGGCGGTCTGTTCTCTGTTGCG201440.2532506513874789No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCAAGAGATTCCATCTGGGGG193560.2433439043018289TruSeq Adapter, Index 9 (97% over 35bp)
TGCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCA187130.23526009925605107No Hit
CGTGCGCGAACGCACGGCACGGATGGCCATCCATCTCGACCTGACGTTGC186930.2350086589746894No Hit
CCCGATGAGGCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGTCT182100.22893637617980495No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCAGGAGATTCCATCTGGGGG166690.2095629025008879TruSeq Adapter, Index 9 (97% over 35bp)
CCACGTTAGTACGCGATTCACGCGGCCACGCGCTGAACCTTGCCAGCCTT166420.20922345812104964No Hit
GTTGCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTC162570.20438323270483738No Hit
CGCGTCTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAACC157990.19862525026165503No Hit
GTGCGCGAACGCACGGCACGGATGGCCATCCATCTCGACCTGACGTTGCC154400.194111897211213No Hit
GCCGCGTCTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAA146590.18429315422403958No Hit
GGGGAATTGAACCCCGGTCCGATGACCGAGCCCACAGTCTTCTACGTGCG146040.18360169345029495No Hit
CCGCGTCTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAAC139080.1748515716589087No Hit
GCGATTTTTCTGCCCCCATCGATGTCGCAGACAACTGATGGCGAGCATAT136910.17212344460613452No Hit
CACGTTAGTACGCGATTCACGCGGCCACGCGCTGAACCTTGCCAGCCTTG130300.16381334330713115No Hit
GTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCACGTCCGGC126880.15951371449584653No Hit
CCGTGCGCGAACGCACGGCACGGATGGCCATCCATCTCGACCTGACGTTG126870.15950114248177844No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTGGGG126090.15852052538446793TruSeq Adapter, Index 7 (97% over 38bp)
GCGGGGAATTGAACCCCGGTCCGATGACCGAGCCCACAGTCTTCTACGTG120070.1509521729154815No Hit
CCACAGTCTTCTACGTGCGTAGTCTGCTGGCCAAGTTGGCGATTTTTCTG118690.14921723497408593No Hit
CCCCGCTACATTGTCGGCGCGGAACCACTAGACCAGTGAGCTGTTACGCA117630.14788460148286905No Hit
TCCCGATGAGGCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGTC116910.14697941646996704No Hit
CCGCCGTTTCACCCTTACCCGTCGAGGACGGGCGGTCTGTTCTCTGTTGC111040.13959964421200188No Hit
GGCGCGGGGAATTGAACCCCGGTCCGATGACCGAGCCCACAGTCTTCTAC108750.1367206529904107No Hit
TCCCGACTTTCGTCCCTGCTCGGACCGTCGTCCTCGCAGTCAAGCCCGCT108620.1365572168075256No Hit
GGAGACGCGATCGGCAGACCATGCGCGTTCAGGTACGGGGCCCTCACCCT106800.13426911024713437No Hit
CGATGAGGCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGTCTCT106040.13331363717796002No Hit
CGACTTTCGTCCCTGCTCGGACCGTCGTCCTCGCAGTCAAGCCCGCTTGT101920.1281339673819095No Hit
CTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAACCACTAG99600.12521726011811407No Hit
CTCCTACCCAGTACAAAATACTGCCGCGTCTTCGGTGGTGTGCTTGAGCC98920.12436236316148438No Hit
CGATGACCGAGCCCACAGTCTTCTACGTGCGTAGTCTGCTGGCCAAGTTG98070.12329374196569726No Hit
CCGATGAGGCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGTCTC90530.11381444335836213No Hit
GTCCCGACTTTCGTCCCTGCTCGGACCGTCGTCCTCGCAGTCAAGCCCGC90050.11321098668309411No Hit
CTGGGCTGTTTCCCTTTCGACGACGAAGCTTATCCCCCGCCGACTCACTG89430.1124315218108729No Hit
GTGCGTAGTCTGCTGGCCAAGTTGGCGATTTTTCTGCCCCCATCGATGTC88820.11166462895271981No Hit
GTCGGTTTGGGGTACGGGCGGCTTCGAGCCTGACGCCGAAGCTTTTCTCG86690.10898678995621795No Hit
GCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGTCTCTTACACCG84960.10681183152243948No Hit
CGCCGACTCACTGCCGGGATACGCGTCACCGGTATTCGGAGTTTGGTTGC84820.1066358233254863No Hit
CGCGGGGAATTGAACCCCGGTCCGATGACCGAGCCCACAGTCTTCTACGT84240.10590664650953746No Hit
CTTGATTGCAGGTAAGACTACTTACGCTGGTGGCGGGTCTTACGCAGCAG83810.10536604990460983No Hit
CACGTGTTCTGCATGGGGACTACAGGAACTCGAAGGTTCCACGGAATGCT81880.10293965118946968No Hit
GCCGTTTCACCCTTACCCGTCGAGGACGGGCGGTCTGTTCTCTGTTGCGC81790.10282650306285693No Hit
CTCGGTTTTGGCGGCTGAAAGCCGGTACGGGAATATCGACCCGTTCATCC81540.10251220271115483No Hit
GTTCTGCATGGGGACTACAGGAACTCGAAGGTTCCACGGAATGCTTATCT80690.10144358151536771No Hit
GGCGAGCATATCCGCAGTTAAAGTCCCGATGCAGCCCTGAGGCTCAACTG80670.10141843748723155No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)