FastQCFastQC Report
Tue 4 Mar 2025
DS022_05_S5_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDS022_05_S5_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6856439
Sequences flagged as poor quality0
Sequence length121
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGACTTTCGTCCCTGCTCGGACCGTCGTCCTCGCAGTCAAGCCCGCTT470710.6865225520127868No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTGGGGG255250.3722777960979453TruSeq Adapter, Index 7 (97% over 38bp)
CCCGCTTCGGGTCCAGGACACGCGACTCAAACGCCTTTTAAGACTCGCCT233050.3398994725979477No Hit
CCGACTTTCGTCCCTGCTCGGACCGTCGTCCTCGCAGTCAAGCCCGCTTG226230.329952618261462No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCAAGAGATTCCATCTGGGGG208050.3034373965844369TruSeq Adapter, Index 9 (97% over 35bp)
CTCCCGATGAGGCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGT185270.27021315292092585No Hit
CGCGTCTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAACC178260.25998918680673744No Hit
GGAGGCGCGGGGAATTGAACCCCGGTCCGATGACCGAGCCCACAGTCTTC177250.2585161189357916No Hit
GCCGCGTCTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAA176950.25807857402362944No Hit
CTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCACGT176100.2568388634391701No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCAGGAGATTCCATCTGGGGG172930.25221547220065693TruSeq Adapter, Index 9 (97% over 35bp)
CCGCGTCTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAAC157270.22937562778579376No Hit
GCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCAC138610.20216033424930932No Hit
CCACGTTAGTACGCGATTCACGCGGCCACGCGCTGAACCTTGCCAGCCTT133520.1947366555729585No Hit
GGCGATTTTTCTGCCCCCATCGATGTCGCAGACAACTGATGGCGAGCATA129970.18955904077903998No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTGGGG126990.18521276131822947TruSeq Adapter, Index 7 (97% over 38bp)
CTCCTACCCAGTACAAAATACTGCCGCGTCTTCGGTGGTGTGCTTGAGCC122370.1784745696709327No Hit
CTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAACCACTAG115600.16860063948647394No Hit
CCGCCATCTGCTTGGCCTTGCTCCCGATGAGGCTTGCCATGCGACCTACT112020.16337927020133922No Hit
CACGTTAGTACGCGATTCACGCGGCCACGCGCTGAACCTTGCCAGCCTTG111230.16222706859931227No Hit
CCCCGCTACATTGTCGGCGCGGAACCACTAGACCAGTGAGCTGTTACGCA110590.1612936394533664No Hit
TGCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCA109860.16022894683377187No Hit
GTTGCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTC106610.15548887695201546No Hit
CGTGCGCGAACGCACGGCACGGATGGCCATCCATCTCGACCTGACGTTGC98110.1430917711074218No Hit
CTGGGCTGTTTCCCTTTCGACGACGAAGCTTATCCCCCGCCGACTCACTG92190.13445755150742245No Hit
CCCGATGAGGCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGTCT91350.13323242575336847No Hit
GGGGACTACAGGAACTCGAAGGTTCCACGGAATGCTTATCTTGGAGCAGG91070.13282405050201715No Hit
CGCCGACTCACTGCCGGGATACGCGTCACCGGTATTCGGAGTTTGGTTGC80580.11752456340674802No Hit
GTGCGCGAACGCACGGCACGGATGGCCATCCATCTCGACCTGACGTTGCC78660.11472427596891038No Hit
CTCGGTTTTGGCGGCTGAAAGCCGGTACGGGAATATCGACCCGTTCATCC75040.10944456736215404No Hit
CTTTCGACGACGAAGCTTATCCCCCGCCGACTCACTGCCGGGATACGCGT74950.10931330388850538No Hit
GTCGGTTTGGGGTACGGGCGGCTTCGAGCCTGACGCCGAAGCTTTTCTCG74940.10929871905809999No Hit
GTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCACGTCCGGC74220.10824861126891089No Hit
CCGTGCGCGAACGCACGGCACGGATGGCCATCCATCTCGACCTGACGTTG73970.10788399050877576No Hit
GCGTCTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAACCA73830.10767980288310011No Hit
GGAGACGCGATCGGCAGACCATGCGCGTTCAGGTACGGGGCCCTCACCCT73790.10762146356147848No Hit
TCCCGACTTTCGTCCCTGCTCGGACCGTCGTCCTCGCAGTCAAGCCCGCT70050.10216673698985727No Hit
CTGCCGCGTCTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGG69410.1012333078439114No Hit
GGCGCGGGGAATTGAACCCCGGTCCGATGACCGAGCCCACAGTCTTCTAC69070.10073742361012765No Hit
GGGGAATTGAACCCCGGTCCGATGACCGAGCCCACAGTCTTCTACGTGCG68840.10040197251080334No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)