FastQCFastQC Report
Tue 4 Mar 2025
DS022_04_S4_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDS022_04_S4_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7439129
Sequences flagged as poor quality0
Sequence length121
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTGGGGG405710.5453729865418384TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCAAGAGATTCCATCTGGGGG361330.48571546480777517TruSeq Adapter, Index 9 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTGGGG308790.4150889169955246TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCAGGAGATTCCATCTGGGGG290890.391026960279893TruSeq Adapter, Index 9 (97% over 35bp)
CTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCACGT209350.2814173541015353No Hit
CTCCCGATGAGGCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGT183190.24625194696852282No Hit
GGGGACTACAGGAACTCGAAGGTTCCACGGAATGCTTATCTTGGAGCAGG179290.24100939773997734No Hit
GGCGATTTTTCTGCCCCCATCGATGTCGCAGACAACTGATGGCGAGCATA163050.21917888505495736No Hit
CCCGCTTCGGGTCCAGGACACGCGACTCAAACGCCTTTTAAGACTCGCCT161750.2174313686454422No Hit
CCCGACTTTCGTCCCTGCTCGGACCGTCGTCCTCGCAGTCAAGCCCGCTT160820.21618122229094291No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCAAGAGATTCCATCTGGGG153050.2057364511356101TruSeq Adapter, Index 9 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCAGGAGATTCCATCTGGGG148130.19912277364729125TruSeq Adapter, Index 9 (97% over 35bp)
GGAGGCGCGGGGAATTGAACCCCGGTCCGATGACCGAGCCCACAGTCTTC139000.18684983147892717No Hit
GCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCAC131290.17648571492711043No Hit
CTCCTACCCAGTACAAAATACTGCCGCGTCTTCGGTGGTGTGCTTGAGCC117400.15781417421313704No Hit
GTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCACGTCCGGC113380.15241031577755945No Hit
GTTCTGCATGGGGACTACAGGAACTCGAAGGTTCCACGGAATGCTTATCT109490.14718120898293335No Hit
CCGCCATCTGCTTGGCCTTGCTCCCGATGAGGCTTGCCATGCGACCTACT103640.1393173851401152No Hit
CGCGTCTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAACC102540.13783871740898698No Hit
CACGTGTTCTGCATGGGGACTACAGGAACTCGAAGGTTCCACGGAATGCT96050.1291145777953306No Hit
CGTGCGCGAACGCACGGCACGGATGGCCATCCATCTCGACCTGACGTTGC95180.1279450860443474No Hit
TGCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCA92460.12428874401828494No Hit
CCGCGTCTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAAC86600.11641147774154743No Hit
CCCGATGAGGCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGTCT83450.11217711105695305No Hit
CCGACTTTCGTCCCTGCTCGGACCGTCGTCCTCGCAGTCAAGCCCGCTTG83300.11197547454816284No Hit
GCCGCGTCTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAA83250.11190826237856609No Hit
CCACGTGTTCTGCATGGGGACTACAGGAACTCGAAGGTTCCACGGAATGC82600.11103450417380852No Hit
GTTGCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTC79990.10752602892085888No Hit
GTGCGCGAACGCACGGCACGGATGGCCATCCATCTCGACCTGACGTTGCC79490.10685390722489152No Hit
GGGGAATTGAACCCCGGTCCGATGACCGAGCCCACAGTCTTCTACGTGCG78430.10542900922944069No Hit
CCACGTTAGTACGCGATTCACGCGGCCACGCGCTGAACCTTGCCAGCCTT78380.10536179705984397No Hit
CTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAACCACTAG76040.1022162675227167No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)