FastQCFastQC Report
Tue 4 Mar 2025
DS022_03_S3_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDS022_03_S3_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6909450
Sequences flagged as poor quality0
Sequence length121
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTACCCAGTACAAAATACTGCCGCGTCTTCGGTGGTGTGCTTGAGCC395210.5719847455296732No Hit
CGCGTCTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAACC339660.49158760827562253No Hit
CCGCGTCTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAAC296990.4298316074361925No Hit
GCCGCGTCTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAA284130.41121941688556973No Hit
CTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAACCACTAG256390.3710715035205407No Hit
CCCGACTTTCGTCCCTGCTCGGACCGTCGTCCTCGCAGTCAAGCCCGCTT232540.33655356070309506No Hit
CCCCGCTACATTGTCGGCGCGGAACCACTAGACCAGTGAGCTGTTACGCA195880.28349579199502134No Hit
CTCTCCTACCCAGTACAAAATACTGCCGCGTCTTCGGTGGTGTGCTTGAG193380.27987755899528904No Hit
CCCGCTTCGGGTCCAGGACACGCGACTCAAACGCCTTTTAAGACTCGCCT191970.27783687558344006No Hit
CTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCACGT190810.2761580154715643No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTGGGG175410.25386970019321364TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTGGGGG166270.24064144034619253TruSeq Adapter, Index 7 (97% over 38bp)
GGGGACTACAGGAACTCGAAGGTTCCACGGAATGCTTATCTTGGAGCAGG153990.2228686798515077No Hit
CTCCCGATGAGGCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGT151470.2192215009877776No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCAAGAGATTCCATCTGGGGG143230.2072958050206601TruSeq Adapter, Index 9 (97% over 35bp)
CTGCATTCTCACTCCCATGAAGTCCACGGCGCGTTCACACGGCCGCTTCG141990.20550116145279293No Hit
GCGTCTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAACCA128010.18526800251829018No Hit
GTGGGTTCGGTCCTCCACGCGGTCTTACCCGCGCTTCAACCTGCTCAGGG126240.18270629355447973No Hit
GCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCAC123690.17901569589475283No Hit
CCCAGTACAAAATACTGCCGCGTCTTCGGTGGTGTGCTTGAGCCCCGCTA123370.1785525620707871No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCAGGAGATTCCATCTGGGGG122960.17795917185883103TruSeq Adapter, Index 9 (97% over 35bp)
CTGGGCTGTTTCCCTTTCGACGACGAAGCTTATCCCCCGCCGACTCACTG115630.16735051270361606No Hit
CTCGGTTTTGGCGGCTGAAAGCCGGTACGGGAATATCGACCCGTTCATCC115430.16706105406363747No Hit
CCGACTTTCGTCCCTGCTCGGACCGTCGTCCTCGCAGTCAAGCCCGCTTG112860.16334151053991272No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTATCTCAGTCCCAATGT111070.16075085571210443No Hit
CCTGGCTGTCTATGCGACTCCACATCCTTTCCCACTTAGCACACGCTTCG108090.1564379219764236No Hit
CTGCCGCGTCTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGG107360.1553813979405018No Hit
GTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCACGTCCGGC106030.15345649798464422No Hit
GGCGATTTTTCTGCCCCCATCGATGTCGCAGACAACTGATGGCGAGCATA103600.14993957550890447No Hit
GTTCTGCATGGGGACTACAGGAACTCGAAGGTTCCACGGAATGCTTATCT103030.1491146183849655No Hit
GGAGGCGCGGGGAATTGAACCCCGGTCCGATGACCGAGCCCACAGTCTTC102350.14813045900903835No Hit
CTACCCAGTACAAAATACTGCCGCGTCTTCGGTGGTGTGCTTGAGCCCCG99320.14374516061336284No Hit
GTCGGTTTGGGGTACGGGCGGCTTCGAGCCTGACGCCGAAGCTTTTCTCG97980.14180578772550637No Hit
GTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAACCACTAGACCAGTGA97950.14176236892950958No Hit
GGAGACGCGATCGGCAGACCATGCGCGTTCAGGTACGGGGCCCTCACCCT92110.13331017664213504No Hit
TGCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCA89320.12927222861443385No Hit
CCGCTACATTGTCGGCGCGGAACCACTAGACCAGTGAGCTGTTACGCACT87930.12726049106658271No Hit
GTTGCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTC85520.12377251445484083No Hit
CACGTGTTCTGCATGGGGACTACAGGAACTCGAAGGTTCCACGGAATGCT84670.12254231523493186No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCAGGAGATTCCATCTGGGG84120.12174630397499078TruSeq Adapter, Index 9 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCAAGAGATTCCATCTGGGG82080.11879382584720927TruSeq Adapter, Index 9 (97% over 35bp)
CCTACCCAGTACAAAATACTGCCGCGTCTTCGGTGGTGTGCTTGAGCCCC77360.11196260194371477No Hit
CCGCCATCTGCTTGGCCTTGCTCCCGATGAGGCTTGCCATGCGACCTACT75940.10990744559986686No Hit
CCCGCAACACCCCGCGCGCAACCCCCGACGGGTATCACACGCGCAAGGTT75890.1098350809398722No Hit
CCACGTGTTCTGCATGGGGACTACAGGAACTCGAAGGTTCCACGGAATGC74570.10792465391601357No Hit
CGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAACCACTAGACC73920.10698391333608319No Hit
CTCGCCTTCGCTACGCATACCCCACACGGGTTATGCTCGCCACATGCCAC72850.10543530961219778No Hit
GCTAGATCATCCCGCTTCGGGTCCAGGACACGCGACTCAAACGCCTTTTA72420.10481297353624383No Hit
GGCGGCTGAAAGCCGGTACGGGAATATCGACCCGTTCATCCATTCGACTA70260.10168682022447517No Hit
CTGGCTGTCTATGCGACTCCACATCCTTTCCCACTTAGCACACGCTTCGG69630.10077502550854264No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)