FastQCFastQC Report
Tue 4 Mar 2025
DS022_02_S2_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDS022_02_S2_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7697380
Sequences flagged as poor quality0
Sequence length121
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGCTTCGGGTCCAGGACACGCGACTCAAACGCCTTTTAAGACTCGCCT393380.5110570090082599No Hit
GGCGATTTTTCTGCCCCCATCGATGTCGCAGACAACTGATGGCGAGCATA348480.45272547282322034No Hit
CTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCACGT324550.42163697258028054No Hit
CTCCCGATGAGGCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGT212110.2755612948821547No Hit
GCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCAC210540.27352163983069566No Hit
GGAGGCGCGGGGAATTGAACCCCGGTCCGATGACCGAGCCCACAGTCTTC206840.2687148094546456No Hit
GGCCTTTCACCCCTAGCCCCAAGTCATCCCCCCGGTTTTCAACCCAGGTG197490.2565678191800327No Hit
GTTGCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTC195840.25442423266098335No Hit
GGGGACTACAGGAACTCGAAGGTTCCACGGAATGCTTATCTTGGAGCAGG190670.2477076615679621No Hit
CCACGTTAGTACGCGATTCACGCGGCCACGCGCTGAACCTTGCCAGCCTT189670.2464085182230837No Hit
CCCGACTTTCGTCCCTGCTCGGACCGTCGTCCTCGCAGTCAAGCCCGCTT180580.23459930521813918No Hit
TGCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCA176060.22872717729928885No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTGGGGG175840.22844136576341562TruSeq Adapter, Index 7 (97% over 38bp)
CCACAGTCTTCTACGTGCGTAGTCTGCTGGCCAAGTTGGCGATTTTTCTG168630.21907454224684245No Hit
GTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCACGTCCGGC160900.2090321641909325No Hit
CTCCTACCCAGTACAAAATACTGCCGCGTCTTCGGTGGTGTGCTTGAGCC160750.20883729268920073No Hit
GTCGGTTTGGGGTACGGGCGGCTTCGAGCCTGACGCCGAAGCTTTTCTCG150230.1951703047010801No Hit
CGCGTCTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAACC149810.1946246644962312No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCAAGAGATTCCATCTGGGGG149250.1938971442230993TruSeq Adapter, Index 9 (97% over 35bp)
CACGTTAGTACGCGATTCACGCGGCCACGCGCTGAACCTTGCCAGCCTTG143730.1867258729593706No Hit
GGAGACGCGATCGGCAGACCATGCGCGTTCAGGTACGGGGCCCTCACCCT138610.18007425903359325No Hit
GCCTTTCACCCCTAGCCCCAAGTCATCCCCCCGGTTTTCAACCCAGGTGG133660.17364349947644522No Hit
GTGGGTTCGGTCCTCCACGCGGTCTTACCCGCGCTTCAACCTGCTCAGGG122240.15880728247793405No Hit
CCGCGTCTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAAC120270.15624797008852362No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCAGGAGATTCCATCTGGGGG117150.15219464285250306TruSeq Adapter, Index 9 (97% over 35bp)
CTGGGCTGTTTCCCTTTCGACGACGAAGCTTATCCCCCGCCGACTCACTG115780.15041481647001967No Hit
CGGGCGGCTTCGAGCCTGACGCCGAAGCTTTTCTCGGCAGCCGGGATCAC115440.149973107732761No Hit
CTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAACCACTAG114360.14857003292029236No Hit
CGATGACCGAGCCCACAGTCTTCTACGTGCGTAGTCTGCTGGCCAAGTTG112730.1464524292681406No Hit
CTTTCGACGACGAAGCTTATCCCCCGCCGACTCACTGCCGGGATACGCGT109290.14198337616175893No Hit
GGGGAATTGAACCCCGGTCCGATGACCGAGCCCACAGTCTTCTACGTGCG109140.14178850466002718No Hit
GCGATTTTTCTGCCCCCATCGATGTCGCAGACAACTGATGGCGAGCATAT108980.14158064172484663No Hit
GCTAGATCATCCCGCTTCGGGTCCAGGACACGCGACTCAAACGCCTTTTA108280.14067124138343176No Hit
GCCGCGTCTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAA102770.13351296155315184No Hit
CCCCGCTACATTGTCGGCGCGGAACCACTAGACCAGTGAGCTGTTACGCA101770.13221381820827346No Hit
CACGGTTTCAGAGACTGTTTCACTCCCCTCCCGGGGTACTTTTCACCTTT101660.13207091244033684No Hit
CTCTCCTACCCAGTACAAAATACTGCCGCGTCTTCGGTGGTGTGCTTGAG101470.13182407520480993No Hit
CTCGCCTTCGCTACGCATACCCCACACGGGTTATGCTCGCCACATGCCAC100570.13065484619441942No Hit
GTTCTGCATGGGGACTACAGGAACTCGAAGGTTCCACGGAATGCTTATCT95580.12417212090347625No Hit
GGCGGATTCGCACGGGGTTCCACGGGACCCGTGCTACTTGGGAGACGCGA94860.12323673769516381No Hit
CACGTGTTCTGCATGGGGACTACAGGAACTCGAAGGTTCCACGGAATGCT94850.12322374626171503No Hit
GTGCGTAGTCTGCTGGCCAAGTTGGCGATTTTTCTGCCCCCATCGATGTC92930.12072939103954852No Hit
CTCATTTTTCGATAGGCACGCCGTCACCCCACAAAAGGGCTCCGACGGAT92650.12036563090298258No Hit
TCCCGCTTCGGGTCCAGGACACGCGACTCAAACGCCTTTTAAGACTCGCC91330.11865076168774309No Hit
TGGCCTTTCACCCCTAGCCCCAAGTCATCCCCCCGGTTTTCAACCCAGGT90300.11731264404251836No Hit
CCGACTTTCGTCCCTGCTCGGACCGTCGTCCTCGCAGTCAAGCCCGCTTG88820.11538991189209834No Hit
GTCAGACAGGAATATTCAGGCTTACCCAACGGTCTGGGCGGATTCGCACG87890.11418170858136145No Hit
CATCCATCTCGACCTGACGTTGCCGCCAGGCTCTAGCAGCCTACCCGATG86690.11262273656750738No Hit
CGCACGGTTTCAGAGACTGTTTCACTCCCCTCCCGGGGTACTTTTCACCT86630.11254478796681468No Hit
CTTCGCTACGCATACCCCACACGGGTTATGCTCGCCACATGCCACTGACT85650.11127162748883386No Hit
CTAGATCATCCCGCTTCGGGTCCAGGACACGCGACTCAAACGCCTTTTAA84890.1102842785467263No Hit
CCACGTGTTCTGCATGGGGACTACAGGAACTCGAAGGTTCCACGGAATGC84240.10943983537255533No Hit
CCCGATGAGGCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGTCT81400.10575026827310072No Hit
CGGCAGACCATGCGCGTTCAGGTACGGGGCCCTCACCCTCTGCGGCCCGG80150.10412633909200274No Hit
CTCGGTTTTGGCGGCTGAAAGCCGGTACGGGAATATCGACCCGTTCATCC79660.10348975885301231No Hit
GCACGGTTTCAGAGACTGTTTCACTCCCCTCCCGGGGTACTTTTCACCTT79510.10329488735128056No Hit
CCGCCATCTGCTTGGCCTTGCTCCCGATGAGGCTTGCCATGCGACCTACT78330.10176189820432406No Hit
CTCAGGGCTAGATCATCCCGCTTCGGGTCCAGGACACGCGACTCAAACGC77730.10098241219739704No Hit
TTCCCTTTCGACGACGAAGCTTATCCCCCGCCGACTCACTGCCGGGATAC77650.10087848072980676No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)