FastQCFastQC Report
Tue 4 Mar 2025
DS020_08_S20_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDS020_08_S20_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22273391
Sequences flagged as poor quality0
Sequence length121
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTGGGGG5701302.5596910681449447TruSeq Adapter, Index 13 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTGGGG1097580.49277633567336016TruSeq Adapter, Index 13 (97% over 38bp)
CTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCACGT927700.4165059554694658No Hit
GCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCAC713330.3202610684650577No Hit
GGGGACTACAGGAACTCGAAGGTTCCACGGAATGCTTATCTTGGAGCAGG697480.3131449539946567No Hit
TGCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCA621750.27914474271115697No Hit
CTCCCGATGAGGCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGT604940.27159762067661813No Hit
GTTGCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTC545690.24499637257748494No Hit
GGCGATTTTTCTGCCCCCATCGATGTCGCAGACAACTGATGGCGAGCATA542680.24364498427742773No Hit
GTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCACGTCCGGC473450.21256305337611142No Hit
GTCGGTTTGGGGTACGGGCGGCTTCGAGCCTGACGCCGAAGCTTTTCTCG390320.17524049211904916No Hit
CCCGCTTCGGGTCCAGGACACGCGACTCAAACGCCTTTTAAGACTCGCCT341040.15311543715997264No Hit
CCGCCATCTGCTTGGCCTTGCTCCCGATGAGGCTTGCCATGCGACCTACT340580.15290891270215656No Hit
GGAGGCGCGGGGAATTGAACCCCGGTCCGATGACCGAGCCCACAGTCTTC335890.1508032611648581No Hit
CTCGGTTTTGGCGGCTGAAAGCCGGTACGGGAATATCGACCCGTTCATCC325940.1463360473490543No Hit
CTTGATTGCAGGTAAGACTACTTACGCTGGTGGCGGGTCTTACGCAGCAG302910.13599635547187225No Hit
CGTGCGCGAACGCACGGCACGGATGGCCATCCATCTCGACCTGACGTTGC298380.13396253852859674No Hit
GTTCTGCATGGGGACTACAGGAACTCGAAGGTTCCACGGAATGCTTATCT293710.13186586631555114No Hit
CACGTGTTCTGCATGGGGACTACAGGAACTCGAAGGTTCCACGGAATGCT291420.1308377336885973No Hit
GTGCGCGAACGCACGGCACGGATGGCCATCCATCTCGACCTGACGTTGCC271370.12183596112509316No Hit
CCCGATGAGGCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGTCT268270.12044416586589801No Hit
CGGGCGGCTTCGAGCCTGACGCCGAAGCTTTTCTCGGCAGCCGGGATCAC249130.11185095255589955No Hit
CGCCGTTTCACCCTTACCCGTCGAGGACGGGCGGTCTGTTCTCTGTTGCG243440.10929633480595749No Hit
GGGGAATTGAACCCCGGTCCGATGACCGAGCCCACAGTCTTCTACGTGCG241300.10833554711090017No Hit
CCCTGGTCTGTGCCACCCCCAAAAGCTCCCACAGCAAGTGTGTTCACCAT238350.10701109678360156No Hit
CCACGTGTTCTGCATGGGGACTACAGGAACTCGAAGGTTCCACGGAATGC237710.10672375840750967No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)