FastQCFastQC Report
Tue 4 Mar 2025
DS020_06_S18_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDS020_06_S18_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6808682
Sequences flagged as poor quality0
Sequence length121
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTGGGGG589990.8665260031236589TruSeq Adapter, Index 13 (97% over 38bp)
CTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCACGT349460.5132564569765485No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTGGGG347000.5096434229120996TruSeq Adapter, Index 13 (97% over 38bp)
GGCGATTTTTCTGCCCCCATCGATGTCGCAGACAACTGATGGCGAGCATA274480.4031323536625738No Hit
GGGGACTACAGGAACTCGAAGGTTCCACGGAATGCTTATCTTGGAGCAGG271990.399475258207095No Hit
GCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCAC245190.3601137488870827No Hit
CTCCCGATGAGGCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGT245150.36005500036570953No Hit
GGAGGCGCGGGGAATTGAACCCCGGTCCGATGACCGAGCCCACAGTCTTC206800.3037298554991994No Hit
TGCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCA204520.3003811897809297No Hit
GTTGCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTC201980.2966506586737345No Hit
GTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCACGTCCGGC183930.2701403884040993No Hit
GTTCTGCATGGGGACTACAGGAACTCGAAGGTTCCACGGAATGCTTATCT183920.27012570127375607No Hit
CACGTGTTCTGCATGGGGACTACAGGAACTCGAAGGTTCCACGGAATGCT155820.22885486500911628No Hit
GGGGAATTGAACCCCGGTCCGATGACCGAGCCCACAGTCTTCTACGTGCG134160.1970425406855541No Hit
CCACGTGTTCTGCATGGGGACTACAGGAACTCGAAGGTTCCACGGAATGC131650.19335607096938878No Hit
CTTGATTGCAGGTAAGACTACTTACGCTGGTGGCGGGTCTTACGCAGCAG126460.18573345032122224No Hit
CGATGACCGAGCCCACAGTCTTCTACGTGCGTAGTCTGCTGGCCAAGTTG121090.17784646132687648No Hit
CCGCCATCTGCTTGGCCTTGCTCCCGATGAGGCTTGCCATGCGACCTACT120760.17736178602554797No Hit
CCCGCTTCGGGTCCAGGACACGCGACTCAAACGCCTTTTAAGACTCGCCT108760.15973722961360215No Hit
CTCGGTTTTGGCGGCTGAAAGCCGGTACGGGAATATCGACCCGTTCATCC105630.15514015781615298No Hit
CCACAGTCTTCTACGTGCGTAGTCTGCTGGCCAAGTTGGCGATTTTTCTG105630.15514015781615298No Hit
CGTGCGCGAACGCACGGCACGGATGGCCATCCATCTCGACCTGACGTTGC104960.15415612008315266No Hit
GTCGGTTTGGGGTACGGGCGGCTTCGAGCCTGACGCCGAAGCTTTTCTCG103040.15133619105724133No Hit
CCCGATGAGGCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGTCT101790.1495002997643303No Hit
GCGGGGAATTGAACCCCGGTCCGATGACCGAGCCCACAGTCTTCTACGTG96970.14242110293886542No Hit
CGCGGATATGTCGATGTTTCGTCTTGTGGCCAGACAATGCTCATTGCCGT96430.14162799790032785No Hit
GCGATTTTTCTGCCCCCATCGATGTCGCAGACAACTGATGGCGAGCATAT90790.13334445638671333No Hit
GTGCGCGAACGCACGGCACGGATGGCCATCCATCTCGACCTGACGTTGCC90760.13330039499568344No Hit
GGCGCGGGGAATTGAACCCCGGTCCGATGACCGAGCCCACAGTCTTCTAC89670.13169949778826504No Hit
CGCGTCTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAACC86060.12639744373433803No Hit
TGCATGGGGACTACAGGAACTCGAAGGTTCCACGGAATGCTTATCTTGGA81300.11940636969093284No Hit
CCACGTTAGTACGCGATTCACGCGGCCACGCGCTGAACCTTGCCAGCCTT78610.11545553162858832No Hit
TCCCGATGAGGCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGTC74200.10897850714719824No Hit
GTGCGTAGTCTGCTGGCCAAGTTGGCGATTTTTCTGCCCCCATCGATGTC72160.10598233255716745No Hit
CCGTGCGCGAACGCACGGCACGGATGGCCATCCATCTCGACCTGACGTTG71970.10570327708064498No Hit
CGCGGGGAATTGAACCCCGGTCCGATGACCGAGCCCACAGTCTTCTACGT71880.10557109290755537No Hit
GCCGCGTCTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAA71130.10446955813180876No Hit
ATCCATACCACCCCGGCACCCACCGCACGACCGGCAGGGCAAAGCCAACG71080.10439612248009232No Hit
CGGGCGGCTTCGAGCCTGACGCCGAAGCTTTTCTCGGCAGCCGGGATCAC68970.10129713797765853No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)