FastQCFastQC Report
Tue 4 Mar 2025
DS020_05_S17_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDS020_05_S17_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61742638
Sequences flagged as poor quality0
Sequence length121
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTGGGGG4204620.6809913110612474TruSeq Adapter, Index 13 (97% over 38bp)
CTCGGTTTTGGCGGCTGAAAGCCGGTACGGGAATATCGACCCGTTCATCC3699820.5992325757121035No Hit
GTCGGTTTGGGGTACGGGCGGCTTCGAGCCTGACGCCGAAGCTTTTCTCG3383130.5479406305898364No Hit
CTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCACGT2786410.4512942903411416No Hit
CCCTGGTCTGTGCCACCCCCAAAAGCTCCCACAGCAAGTGTGTTCACCAT2513470.40708821025755326No Hit
CGCGGATATGTCGATGTTTCGTCTTGTGGCCAGACAATGCTCATTGCCGT2351920.38092314746901484No Hit
CTGGTCTGTGCCACCCCCAAAAGCTCCCACAGCAAGTGTGTTCACCATCG2226120.36054824868351104No Hit
CGGGCGGCTTCGAGCCTGACGCCGAAGCTTTTCTCGGCAGCCGGGATCAC1948200.3155355947052343No Hit
GCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCAC1930290.31263484401168606No Hit
CCTCGGTTTTGGCGGCTGAAAGCCGGTACGGGAATATCGACCCGTTCATC1901520.30797517916225087No Hit
CCCGCTTCGGGTCCAGGACACGCGACTCAAACGCCTTTTAAGACTCGCCT1887030.30562834066144046No Hit
CGTCCTTCTGCGCTTAACGAGCATCTTTACTCGTACTGCAATTTCGCCGA1694170.27439222794464985No Hit
CGCGTCTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAACC1636610.2650696589931904No Hit
TGCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCA1629140.26385979815115773No Hit
CTCCCGATGAGGCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGT1528750.24760036978011857No Hit
GGCGGCTGAAAGCCGGTACGGGAATATCGACCCGTTCATCCATTCGACTA1471270.23829075783901557No Hit
GGCGATTTTTCTGCCCCCATCGATGTCGCAGACAACTGATGGCGAGCATA1464330.23716673719059428No Hit
CACCAGTGTCGGTTTGGGGTACGGGCGGCTTCGAGCCTGACGCCGAAGCT1445360.23409430610982318No Hit
GTTGCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTC1442030.2335549705537363No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTGGGG1363610.2208538611518348TruSeq Adapter, Index 13 (97% over 38bp)
CTGGGCTGTTTCCCTTTCGACGACGAAGCTTATCCCCCGCCGACTCACTG1332390.21579738786023364No Hit
CTCGCCTTCGCTACGCATACCCCACACGGGTTATGCTCGCCACATGCCAC1263800.20468837110587984No Hit
CCGCGTCTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAAC1243100.2013357446761507No Hit
GCCGCGTCTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAA1225030.19840907996188953No Hit
GGCCTTTCACCCCTAGCCCCAAGTCATCCCCCCGGTTTTCAACCCAGGTG1219590.19752800325765155No Hit
CTCATTTTTCGATAGGCACGCCGTCACCCCACAAAAGGGCTCCGACGGAT1189500.1926545477373351No Hit
CTCCTACCCAGTACAAAATACTGCCGCGTCTTCGGTGGTGTGCTTGAGCC1184080.1917767102856862No Hit
CCCCGCTACATTGTCGGCGCGGAACCACTAGACCAGTGAGCTGTTACGCA1180860.19125519061883944No Hit
CGCCACATGCCACTGACTCGCAGACTCATTTTTCGATAGGCACGCCGTCA1171230.18969549049718284No Hit
CTTCGGTGGTGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAACCACTAG1164700.18863787452683833No Hit
GCGGATATGTCGATGTTTCGTCTTGTGGCCAGACAATGCTCATTGCCGTC1135670.18393609939374472No Hit
GTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCACGTCCGGC1125030.18221281701633804No Hit
CGGGGGTCTCCCTTAAACCGAAGGAACGGGAGTGATTTGCCGAGTTCCTT1108300.1795031822255473No Hit
GTTTTTGGTAAACAGTCGCTACCCCCTGGTCTGTGCCACCCCCAAAAGCT1100780.1782852232520418No Hit
GCGGACGCGGATATGTCGATGTTTCGTCTTGTGGCCAGACAATGCTCATT1017420.16478401846063007No Hit
CTTCGCTACGCATACCCCACACGGGTTATGCTCGCCACATGCCACTGACT1005110.16279025849203269No Hit
GCCGAGTTCCTTGACCAGGATTCGCTCGATCGCTTTGGTATTCTCTACCT985030.15953804889256595No Hit
CTGCATTCTCACTCCCATGAAGTCCACGGCGCGTTCACACGGCCGCTTCG976270.15811925625853562No Hit
CTTCTGCGTCACTCCTGCGCTGACCTACTACAAGGATCGGTCCCAACAGG964800.15626154489868088No Hit
CCTGGCTGTCTATGCGACTCCACATCCTTTCCCACTTAGCACACGCTTCG949690.15381428956760806No Hit
GGAGGCGCGGGGAATTGAACCCCGGTCCGATGACCGAGCCCACAGTCTTC947840.15351465870311534No Hit
CTCCTGCGCTGACCTACTACAAGGATCGGTCCCAACAGGCCAGGGCATCC934770.15139780713613177No Hit
GTCGCTACCCCCTGGTCTGTGCCACCCCCAAAAGCTCCCACAGCAAGTGT922990.1494898873611458No Hit
GCCTTTCACCCCTAGCCCCAAGTCATCCCCCCGGTTTTCAACCCAGGTGG922570.1494218630567745No Hit
GCGGCTTCGAGCCTGACGCCGAAGCTTTTCTCGGCAGCCGGGATCACCGG908730.14718030026511017No Hit
GTCCTTCTGCGCTTAACGAGCATCTTTACTCGTACTGCAATTTCGCCGAG905630.14667821611379805No Hit
GCCACATGCCACTGACTCGCAGACTCATTTTTCGATAGGCACGCCGTCAC903470.14632837683417416No Hit
CCAGTGTCGGTTTGGGGTACGGGCGGCTTCGAGCCTGACGCCGAAGCTTT893830.14476705708622298No Hit
CCCGACTCACCCAGGGACGATGAACGTGGCCCTGGAACCCTTGGTCATCC893550.14472170754997543No Hit
CCGGGACGTAATGACCCGACGCTGCACCCAAATGCATTTCGGAGAGAACC890530.1442325804090198No Hit
CACGGTTTCAGAGACTGTTTCACTCCCCTCCCGGGGTACTTTTCACCTTT884650.14328024014782134No Hit
GTTTTGGCGGCTGAAAGCCGGTACGGGAATATCGACCCGTTCATCCATTC876930.14202988864842478No Hit
GTACGGGCGGCTTCGAGCCTGACGCCGAAGCTTTTCTCGGCAGCCGGGAT876750.14200073537512278No Hit
CTGTGTTTTTGGTAAACAGTCGCTACCCCCTGGTCTGTGCCACCCCCAAA875240.14175617180464495No Hit
CTGCGTCACTCCTGCGCTGACCTACTACAAGGATCGGTCCCAACAGGCCA869120.1407649605123772No Hit
CCGGGCAGGCGTCAGTGCATATACAGCGGCTTGCGCCTTCGCATGCACCT868030.1405884212462707No Hit
CCGGGGTCTTTTCGTCCTTCTGCGCTTAACGAGCATCTTTACTCGTACTG865020.1401009137316096No Hit
CCATCATCGCACCTCGGACCCATGCGGGACGGATTTGCCTGTCCCACGCC856210.13867402296610648No Hit
GCTAGATCATCCCGCTTCGGGTCCAGGACACGCGACTCAAACGCCTTTTA855010.1384796678107599No Hit
GGAGACGCGATCGGCAGACCATGCGCGTTCAGGTACGGGGCCCTCACCCT849730.13762450512723476No Hit
GTGGGTTCGGTCCTCCACGCGGTCTTACCCGCGCTTCAACCTGCTCAGGG848320.13739613781970247No Hit
CCTGGTCTGTGCCACCCCCAAAAGCTCCCACAGCAAGTGTGTTCACCATC836790.1355287087020804No Hit
GGGGACTACAGGAACTCGAAGGTTCCACGGAATGCTTATCTTGGAGCAGG825090.13363374593745087No Hit
CCCGGGGTCTTTTCGTCCTTCTGCGCTTAACGAGCATCTTTACTCGTACT818340.13254049818862615No Hit
CGCACGGTTTCAGAGACTGTTTCACTCCCCTCCCGGGGTACTTTTCACCT817960.13247895238943305No Hit
CCGAGTTCCTTGACCAGGATTCGCTCGATCGCTTTGGTATTCTCTACCTG816690.13227325985002455No Hit
ATCCATACCACCCCGGCACCCACCGCACGACCGGCAGGGCAAAGCCAACG814870.13197848786441552No Hit
CCAGGATTCGCTCGATCGCTTTGGTATTCTCTACCTGACCACCAGTGTCG810620.13129014668922956No Hit
GATTTGCCGAGTTCCTTGACCAGGATTCGCTCGATCGCTTTGGTATTCTC800940.1297223484361002No Hit
GTTTGGGGTACGGGCGGCTTCGAGCCTGACGCCGAAGCTTTTCTCGGCAG798870.12938708579312727No Hit
GCCACTGACTCGCAGACTCATTTTTCGATAGGCACGCCGTCACCCCACAA797120.12910365119158013No Hit
CCGCCATCTGCTTGGCCTTGCTCCCGATGAGGCTTGCCATGCGACCTACT786410.12736903143011155No Hit
GCCTTCGCTACGCATACCCCACACGGGTTATGCTCGCCACATGCCACTGA777430.12591460701760102No Hit
CTCGCCTTAGGACCCGACTCACCCAGGGACGATGAACGTGGCCCTGGAAC775860.12566032568935587No Hit
CGGGAGTGATTTGCCGAGTTCCTTGACCAGGATTCGCTCGATCGCTTTGG764130.12376050404584268No Hit
GTCGATGTTTCGTCTTGTGGCCAGACAATGCTCATTGCCGTCTGGTGCCG753300.12200644876883945No Hit
GGCGGCTTCGAGCCTGACGCCGAAGCTTTTCTCGGCAGCCGGGATCACCG751580.12172787304617597No Hit
CAGGAATATTCAGGCTTACCCAACGGTCTGGGCGGATTCGCACGGGGTTC738720.11964503363137803No Hit
CCCTTCTGCGTCACTCCTGCGCTGACCTACTACAAGGATCGGTCCCAACA738650.11963369624731617No Hit
GCACGGTTTCAGAGACTGTTTCACTCCCCTCCCGGGGTACTTTTCACCTT730750.11835419147461759No Hit
CGTCACTCCTGCGCTGACCTACTACAAGGATCGGTCCCAACAGGCCAGGG718800.11641873805262419No Hit
CGTGCGCGAACGCACGGCACGGATGGCCATCCATCTCGACCTGACGTTGC710480.1150712089755543No Hit
CAGCGGACGCGGATATGTCGATGTTTCGTCTTGTGGCCAGACAATGCTCA705540.11427111358604405No Hit
GGCGGATTCGCACGGGGTTCCACGGGACCCGTGCTACTTGGGAGACGCGA698140.11307259012807325No Hit
TCGGTTTTGGCGGCTGAAAGCCGGTACGGGAATATCGACCCGTTCATCCA696310.11277619851616964No Hit
CGCCTTCGCTACGCATACCCCACACGGGTTATGCTCGCCACATGCCACTG691830.11205060593620896No Hit
GCAGGCGTCAGTGCATATACAGCGGCTTGCGCCTTCGCATGCACCTGTGT690000.11175421432430535No Hit
TGGCCTTTCACCCCTAGCCCCAAGTCATCCCCCCGGTTTTCAACCCAGGT689830.11172668067729792No Hit
CCCCTGGTCTGTGCCACCCCCAAAAGCTCCCACAGCAAGTGTGTTCACCA683560.11071117499061184No Hit
CTCGCAGACTCATTTTTCGATAGGCACGCCGTCACCCCACAAAAGGGCTC682360.1105168198352652No Hit
CTGTGCCACCCCCAAAAGCTCCCACAGCAAGTGTGTTCACCATCGGGGGT680440.11020585158671063No Hit
GTCAGACAGGAATATTCAGGCTTACCCAACGGTCTGGGCGGATTCGCACG665720.10782176168112545No Hit
GGGGAATTGAACCCCGGTCCGATGACCGAGCCCACAGTCTTCTACGTGCG664840.10767923456720459No Hit
CTCTCCTACCCAGTACAAAATACTGCCGCGTCTTCGGTGGTGTGCTTGAG662860.10735854856088267No Hit
CTACCCTTCTGCGTCACTCCTGCGCTGACCTACTACAAGGATCGGTCCCA656810.10637867465267681No Hit
CACCCCGGCACCCACCGCACGACCGGCAGGGCAAAGCCAACGACGTCTTC646190.10465863152785924No Hit
CTCGCCACATGCCACTGACTCGCAGACTCATTTTTCGATAGGCACGCCGT645150.1044901903932255No Hit
CTCAGGGCTAGATCATCCCGCTTCGGGTCCAGGACACGCGACTCAAACGC638690.10344391180694289No Hit
CTAGATCATCCCGCTTCGGGTCCAGGACACGCGACTCAAACGCCTTTTAA638520.10341637815993543No Hit
CTTAAACCGAAGGAACGGGAGTGATTTGCCGAGTTCCTTGACCAGGATTC636200.10304062485959864No Hit
CTCGATCGCTTTGGTATTCTCTACCTGACCACCAGTGTCGGTTTGGGGTA634480.10276204913693517No Hit
CCGGCACCCACCGCACGACCGGCAGGGCAAAGCCAACGACGTCTTCCACA631320.10225024722785574No Hit
CCGGCACCGGGCAGGCGTCAGTGCATATACAGCGGCTTGCGCCTTCGCAT631010.10220003881272453No Hit
CCCGATGAGGCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGTCT625670.10133515837143209No Hit
GGGGTACGGGCGGCTTCGAGCCTGACGCCGAAGCTTTTCTCGGCAGCCGG623750.10102419012287749No Hit
CGACGGTCTGGGCTGTTTCCCTTTCGACGACGAAGCTTATCCCCCGCCGA620340.10047189755643417No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)