FastQCFastQC Report
Tue 4 Mar 2025
DS020_03_S15_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDS020_03_S15_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15754786
Sequences flagged as poor quality0
Sequence length121
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTGGGGG3129271.9862345321605765TruSeq Adapter, Index 13 (97% over 38bp)
CTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCACGT849980.5395058999849316No Hit
GCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCAC589150.3739498587921156No Hit
TGCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCA477750.30324118651944876No Hit
GGCGATTTTTCTGCCCCCATCGATGTCGCAGACAACTGATGGCGAGCATA468820.29757306763798635No Hit
GTTGCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTC423260.2686548709706371No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTGGGG388990.24690275069429699TruSeq Adapter, Index 13 (97% over 38bp)
CTCCCGATGAGGCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGT373420.23702003949783892No Hit
GTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCACGTCCGGC319810.20299228437631586No Hit
GGAGGCGCGGGGAATTGAACCCCGGTCCGATGACCGAGCCCACAGTCTTC319490.2027891714936655No Hit
CTCGGTTTTGGCGGCTGAAAGCCGGTACGGGAATATCGACCCGTTCATCC300720.19087533147070357No Hit
CTTGATTGCAGGTAAGACTACTTACGCTGGTGGCGGGTCTTACGCAGCAG275110.1746199535810896No Hit
GTCGGTTTGGGGTACGGGCGGCTTCGAGCCTGACGCCGAAGCTTTTCTCG252990.1605797755678814No Hit
CCCGCTTCGGGTCCAGGACACGCGACTCAAACGCCTTTTAAGACTCGCCT251200.15944361288055578No Hit
GGGGACTACAGGAACTCGAAGGTTCCACGGAATGCTTATCTTGGAGCAGG229050.14538439303459916No Hit
GGGGAATTGAACCCCGGTCCGATGACCGAGCCCACAGTCTTCTACGTGCG228200.14484487444005903No Hit
CGATGACCGAGCCCACAGTCTTCTACGTGCGTAGTCTGCTGGCCAAGTTG207060.13142672962996768No Hit
CCGCCATCTGCTTGGCCTTGCTCCCGATGAGGCTTGCCATGCGACCTACT193120.12257862467951008No Hit
CCACAGTCTTCTACGTGCGTAGTCTGCTGGCCAAGTTGGCGATTTTTCTG183070.11619961070877129No Hit
CCCTGGTCTGTGCCACCCCCAAAAGCTCCCACAGCAAGTGTGTTCACCAT170860.10844958478014237No Hit
CGTGCGCGAACGCACGGCACGGATGGCCATCCATCTCGACCTGACGTTGC167020.10601223018833769No Hit
CCACGTTAGTACGCGATTCACGCGGCCACGCGCTGAACCTTGCCAGCCTT162930.10341619365696239No Hit
CTGGTCTGTGCCACCCCCAAAAGCTCCCACAGCAAGTGTGTTCACCATCG162850.1033654154362998No Hit
GCGATTTTTCTGCCCCCATCGATGTCGCAGACAACTGATGGCGAGCATAT162830.10335272088113415No Hit
GCGGGGAATTGAACCCCGGTCCGATGACCGAGCCCACAGTCTTCTACGTG161410.10245140746437305No Hit
CTCCTACCCAGTACAAAATACTGCCGCGTCTTCGGTGGTGTGCTTGAGCC159930.101512010382115No Hit
CTCGCCTTCGCTACGCATACCCCACACGGGTTATGCTCGCCACATGCCAC158980.10090901901174665No Hit
CCCGATGAGGCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGTCT158270.1004583623033661No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)