FastQCFastQC Report
Tue 4 Mar 2025
DS020_02_S14_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDS020_02_S14_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13433644
Sequences flagged as poor quality0
Sequence length121
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTGGGGG1855511.38124100951313TruSeq Adapter, Index 13 (97% over 38bp)
CTCCCGATGAGGCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGT818340.6091720161707426No Hit
CTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCACGT568980.42354851743875305No Hit
CCGCCATCTGCTTGGCCTTGCTCCCGATGAGGCTTGCCATGCGACCTACT429590.31978664910280485No Hit
GCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCAC402730.29979207428751276No Hit
CGTGCGCGAACGCACGGCACGGATGGCCATCCATCTCGACCTGACGTTGC394310.29352422916671006No Hit
GGCGATTTTTCTGCCCCCATCGATGTCGCAGACAACTGATGGCGAGCATA385400.28689162821346165No Hit
CCCGATGAGGCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGTCT346440.2578898175357334No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTGGGG345790.25740595775799924TruSeq Adapter, Index 13 (97% over 38bp)
GTGCGCGAACGCACGGCACGGATGGCCATCCATCTCGACCTGACGTTGCC344950.2567806620452351No Hit
TGCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCA328500.24453528767027027No Hit
GTTGCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTC292660.21785600392566604No Hit
CCGTGCGCGAACGCACGGCACGGATGGCCATCCATCTCGACCTGACGTTG254360.18934549702225248No Hit
TCCCGATGAGGCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGTC250570.18652422231823323No Hit
CGCCGTTTCACCCTTACCCGTCGAGGACGGGCGGTCTGTTCTCTGTTGCG242190.18028615318375268No Hit
GTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCACGTCCGGC241270.1796013054983443No Hit
GTCGGTTTGGGGTACGGGCGGCTTCGAGCCTGACGCCGAAGCTTTTCTCG230910.17188932504091964No Hit
CGATGAGGCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGTCTCT215380.1603287983513632No Hit
GGAGGCGCGGGGAATTGAACCCCGGTCCGATGACCGAGCCCACAGTCTTC215110.16012781044368898No Hit
CCCGCTTCGGGTCCAGGACACGCGACTCAAACGCCTTTTAAGACTCGCCT177030.131781071465047No Hit
GCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGTCTCTTACACCG176850.13164707952659754No Hit
CTCGGTTTTGGCGGCTGAAAGCCGGTACGGGAATATCGACCCGTTCATCC175010.13027738415578083No Hit
GGGGAATTGAACCCCGGTCCGATGACCGAGCCCACAGTCTTCTACGTGCG173430.12910123269605775No Hit
GGGGACTACAGGAACTCGAAGGTTCCACGGAATGCTTATCTTGGAGCAGG172820.12864714890464568No Hit
CCGATGAGGCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGTCTC168280.125267574457087No Hit
CTCCTACCCAGTACAAAATACTGCCGCGTCTTCGGTGGTGTGCTTGAGCC163460.1216795681052736No Hit
CGATGACCGAGCCCACAGTCTTCTACGTGCGTAGTCTGCTGGCCAAGTTG161560.12026520875497371No Hit
CTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGTCTCTTACACCGC154790.11522562306995779No Hit
CGGGCGGCTTCGAGCCTGACGCCGAAGCTTTTCTCGGCAGCCGGGATCAC141030.10498268377515438No Hit
GCGCGAACGCACGGCACGGATGGCCATCCATCTCGACCTGACGTTGCCGC140850.10484869183670492No Hit
CCACAGTCTTCTACGTGCGTAGTCTGCTGGCCAAGTTGGCGATTTTTCTG138050.10276437279415772No Hit
GCCATCTGCTTGGCCTTGCTCCCGATGAGGCTTGCCATGCGACCTACTGT137930.10267504483519141No Hit
GCGATTTTTCTGCCCCCATCGATGTCGCAGACAACTGATGGCGAGCATAT137540.10238472896855089No Hit
GGCTTGCCATGCGACCTACTGTTACCAGAGGCCCGGTGGTCTCTTACACC136260.10143189740624362No Hit
CTTGGCCTTGCTCCCGATGAGGCTTGCCATGCGACCTACTGTTACCAGAG135750.10105225358063681No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)