FastQCFastQC Report
Tue 4 Mar 2025
DS020_01_S13_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDS020_01_S13_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3942053
Sequences flagged as poor quality0
Sequence length121
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTGGGGG3018797.657913275138614TruSeq Adapter, Index 13 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTGGGG241130.611686347190157TruSeq Adapter, Index 13 (97% over 38bp)
CTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCACGT173230.43944107296375773No Hit
GGCCTTTCACCCCTAGCCCCAAGTCATCCCCCCGGTTTTCAACCCAGGTG119740.303750355461989No Hit
GCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCAC115240.29233498382695516No Hit
GTCGGTTTGGGGTACGGGCGGCTTCGAGCCTGACGCCGAAGCTTTTCTCG104860.26600352658881043No Hit
TGCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCA103060.26143737793479693No Hit
GTTGCCTTTCGTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTC98880.2508337660604766No Hit
CCCGCTTCGGGTCCAGGACACGCGACTCAAACGCCTTTTAAGACTCGCCT93430.23700848263582455No Hit
GCCTTTCACCCCTAGCCCCAAGTCATCCCCCCGGTTTTCAACCCAGGTGG87420.2217626196299238No Hit
CGGGCGGCTTCGAGCCTGACGCCGAAGCTTTTCTCGGCAGCCGGGATCAC74920.19005325397705206No Hit
GGGGACTACAGGAACTCGAAGGTTCCACGGAATGCTTATCTTGGAGCAGG73700.18695841988933176No Hit
CTCGGTTTTGGCGGCTGAAAGCCGGTACGGGAATATCGACCCGTTCATCC70390.17856177986445135No Hit
GTCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCACGTCCGGC68240.1731077689721574No Hit
TGGCCTTTCACCCCTAGCCCCAAGTCATCCCCCCGGTTTTCAACCCAGGT59760.15159613531324923No Hit
GGCGATTTTTCTGCCCCCATCGATGTCGCAGACAACTGATGGCGAGCATA56720.1438844175864708No Hit
CTCATTTTTCGATAGGCACGCCGTCACCCCACAAAAGGGCTCCGACGGAT53940.13683225466527213No Hit
CTGGGCTGTTTCCCTTTCGACGACGAAGCTTATCCCCCGCCGACTCACTG53510.13574145248681335No Hit
CACCAGTGTCGGTTTGGGGTACGGGCGGCTTCGAGCCTGACGCCGAAGCT47470.12041948700334572No Hit
GCTAGATCATCCCGCTTCGGGTCCAGGACACGCGACTCAAACGCCTTTTA46150.11707097799040246No Hit
GGAGACGCGATCGGCAGACCATGCGCGTTCAGGTACGGGGCCCTCACCCT44660.11329122160458015No Hit
CACGGTTTCAGAGACTGTTTCACTCCCCTCCCGGGGTACTTTTCACCTTT42950.1089533803832673No Hit
CTTTCGACGACGAAGCTTATCCCCCGCCGACTCACTGCCGGGATACGCGT42650.10819235560759836No Hit
GGCGGATTCGCACGGGGTTCCACGGGACCCGTGCTACTTGGGAGACGCGA42110.10682251101139432No Hit
CTCGCCTTCGCTACGCATACCCCACACGGGTTATGCTCGCCACATGCCAC41280.10471700913204363No Hit
CTTCTGCGTCACTCCTGCGCTGACCTACTACAAGGATCGGTCCCAACAGG40890.10372767692367404No Hit
CGGCCTTTCACCCCTAGCCCCAAGTCATCCCCCCGGTTTTCAACCCAGGT40120.10177437999945714No Hit
CTTTCACCCCTAGCCCCAAGTCATCCCCCCGGTTTTCAACCCAGGTGGGT39500.10020159546307471No Hit
CCACAGTCTTCTACGTGCGTAGTCTGCTGGCCAAGTTGGCGATTTTTCTG39450.10007475800046321No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)