FastQCFastQC Report
Wed 25 Oct 2023
WM012_70_S63_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWM012_70_S63_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6571626
Sequences flagged as poor quality0
Sequence length120
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTGGGTT418530.6368743443403504TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTGGTTT414340.6304984489379037TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTGGTAT302220.4598861834194459TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT123650.18815739057578748TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTTT85400.1299526175104913TruSeq Adapter, Index 13 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)