FastQCFastQC Report
Wed 25 Oct 2023
WM012_65_S58_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWM012_65_S58_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2665363
Sequences flagged as poor quality0
Sequence length120
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTGGGTT182770.6857227327009492TruSeq Adapter, Index 13 (97% over 38bp)
GGTGATATTCAAAATGCTTATCAAGGTTTTGCGAAACAAAACTACACAAT181790.6820459352065741No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTGGTTT179780.6745047485089273TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTGGTAT133910.5024081147671068TruSeq Adapter, Index 13 (97% over 38bp)
CATACTTTTGACCAGTCAGCTTTTGAGCATCAGTCAAGAGTCGTTGCATT119210.44725615235148086No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT55470.2081142418499844TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTTT37760.14166925855877793TruSeq Adapter, Index 13 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)