FastQCFastQC Report
Wed 25 Oct 2023
WM012_46_S52_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWM012_46_S52_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5630161
Sequences flagged as poor quality0
Sequence length120
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTTT647491.1500381605428334TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT565841.0050156647385395TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGGTT524860.9322291138743635TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTGGTTT425780.756248355952876TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTGGGTT361940.6428590585597819TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTGGTAT244570.43439255111887565TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCGCGGTT74510.1323407980695401TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGGAT70370.12498754476115337TruSeq Adapter, Index 7 (97% over 38bp)
GCACCTTCTGTCGATTCGCTACCCTAAGCGTTACTTAAGCAGTTAGCTCG59970.10651560408308039No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCGGGGTT59510.1056985759377041TruSeq Adapter, Index 7 (97% over 38bp)
GTGCACCTTCTGTCGATTCGCTACCCTAAGCGTTACTTAAGCAGTTAGCT58080.10315868409446906No Hit
CCGTGCACCTTCTGTCGATTCGCTACCCTAAGCGTTACTTAAGCAGTTAG56640.10060103076981279No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)