FastQCFastQC Report
Wed 13 May 2020
D3_CIPB_.forward.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameD3_CIPB_.forward.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6216054
Sequences flagged as poor quality0
Sequence length36-314
%GC36

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAAGC984641.584027423185191No Hit
CAAACAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCTAAC879751.4152869328355255No Hit
GGGGACGTTCATGGATTCGACAGGGGTCCCCCGAGCTCATTAAGCGTGTC843101.3563266985775864No Hit
TCTGGTGACTATAGCAAGGAGGTCACACCTGTTCCCATGCCGAACACAGA550950.8863339990289659No Hit
GTAACACGTGGATAACCTACCTATAAGACTGGGATAACTTCGGGAAACCG343070.551909619832775No Hit
TCAAACAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCTAA322690.5191235468675144No Hit
AGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAAGGATAT313720.5046931702974267No Hit
TTAAAGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAA254020.4086515335934984No Hit
TATGAGTACTTTAACTTATGGATTCAACGGTAATGTTACTGGTGATGATA226930.3650708311092536No Hit
AGTAGAGTTAGTTTCCTTGGACTCAGTGCTATGTATTTTTCTTAATTATC178630.2873688034241659No Hit
AACAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCTAACAG151080.2430480816286345No Hit
AAAACAGACGTTTAGCACAACGTGAAGTTAACAAAGCACCTATGGATGTA126500.20350531060380106No Hit
ACTGAATGACAATATGTCAACGTTAATTCCAAAAACGTAACTATAAGTTA118690.19094106968826205No Hit
ACCAGACTTCGCTACAGTTATTACTATGGATAGAAAAGCATCCAAACAAC109540.17622112034419263No Hit
AACACGTGGATAACCTACCTATAAGACTGGGATAACTTCGGGAAACCGGA102440.16479908314824807No Hit
AGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAAGCTTCGA101660.1635442677943274No Hit
AATCAAACAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCT96130.1546479486825565No Hit
ATATCATTTCAACAATCGGTGACTTAGTAAAATGGATTATCGACACAGTG96010.1544549001665687No Hit
AAAAATAACTGTAAAACATTCCCTTAATAATAAGTATGGTCGTGAGCCCC95760.1540527157582608No Hit
AAGTGAGTAACATTTGCTAGTAGAGTTAGTTTCCTTGGACTCAGTGCTAT94020.15125351227643777No Hit
AAACAGGGCACCCACCTGTATATAACAGGCCGAATGATCAAGCTATTATA86390.13897884413488043No Hit
AGTAGAGTTAGTTTCCTTGGACTCAGTGCTATGTATTTTCTTAATTATCA84640.13616355327672508No Hit
GATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATAT84060.1352304854494507No Hit
CACTAAAAAATAAGATGAATAATTAATTACTTTCATTGTAAATTTGTTAT80050.12877944754019188No Hit
CAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCTAACAGCA77390.12450020543579576No Hit
AACTGAATGCTTAGAGCAATCAATTGTTAAAGAAGCTGCGAAAGGTTACT77380.12448411805946345No Hit
TAGTAGAGTTAGTTTCCTTGGACTCAGTGCTATGTATTTTTCTTAATTAT73430.11812960440819852No Hit
AGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAAGCTT72270.11626346875364983No Hit
CAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAAGGAT71760.11544301256070169No Hit
AATGAATCCTGTCGCTAATGCCGCAGATTCTGATATTAATATTAAAACCG71170.11449385735709504No Hit
TAAAGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAAG68890.11082593555332691No Hit
ATTAAAGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAA67680.10887936301711666No Hit
ACAAGAAACAGCACACATCAATGAATTAACACCTTAGATTCATCATGAGA66520.10701322736256795No Hit
ACTAGATAAGTAAGTAAAATATAGATTTTACCAAGCAAAACCGAGTGAAT65950.10609624691162592No Hit
AAACAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCTAACA65830.10590319839563814No Hit
GGATCACCTCCTTTCTAAGGATATATTCGGAACATCTTCTTCAGAAGATG64010.10297529590315656No Hit
TATGGATTCAACGGTAATGTTACTGGTGATGATACAGGAAAAATTGGCGG63290.1018170048072298No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)