FastQCFastQC Report
Wed 13 May 2020
D3_CIPA_.forward.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameD3_CIPA_.forward.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6144460
Sequences flagged as poor quality0
Sequence length36-315
%GC36

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACGTTCATGGATTCGACAGGGGTCCCCCGAGCTCATTAAGCGTGTC1057231.7206231304296877No Hit
CAAACAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCTAAC937661.5260250697376174No Hit
AAAGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAAGC871651.4185949619657383No Hit
TCTGGTGACTATAGCAAGGAGGTCACACCTGTTCCCATGCCGAACACAGA799651.3014162351126055No Hit
TCAAACAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCTAA357360.5815970809477155No Hit
GTAACACGTGGATAACCTACCTATAAGACTGGGATAACTTCGGGAAACCG353710.5756567704891886No Hit
AGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAAGGATAT316470.5150493289890405No Hit
TTAAAGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAA315280.513112625031329No Hit
TATGAGTACTTTAACTTATGGATTCAACGGTAATGTTACTGGTGATGATA175510.2856394215276851No Hit
AGTAGAGTTAGTTTCCTTGGACTCAGTGCTATGTATTTTTCTTAATTATC144590.2353176682735342No Hit
AACAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCTAACAG134740.2192869674470987No Hit
AAAACAGACGTTTAGCACAACGTGAAGTTAACAAAGCACCTATGGATGTA130620.21258174029939164No Hit
GATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATAT126760.20629965855420979No Hit
ACTGAATGACAATATGTCAACGTTAATTCCAAAAACGTAACTATAAGTTA121540.1978042008573577No Hit
ACCAGACTTCGCTACAGTTATTACTATGGATAGAAAAGCATCCAAACAAC106270.17295254587058911No Hit
AAACAGGGCACCCACCTGTATATAACAGGCCGAATGATCAAGCTATTATA99030.16116957389257966No Hit
AACACGTGGATAACCTACCTATAAGACTGGGATAACTTCGGGAAACCGGA96120.1564336003489322No Hit
AATCAAACAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCT92130.14993994590248777No Hit
GCATTAAGTGGATCATCATATGTGCCGTAGGGTAGCCGAGATTTAGCTAA86910.14144448820563565No Hit
AGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAAGCTTCGA86690.14108644209580662No Hit
TAAAGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAAG83450.13581339938741566No Hit
AGTAGAGTTAGTTTCCTTGGACTCAGTGCTATGTATTTTCTTAATTATCA83250.13548790292393473No Hit
AGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAAGCTT80780.1314680215999453No Hit
CACTAAAAAATAAGATGAATAATTAATTACTTTCATTGTAAATTTGTTAT79110.1287501261298796No Hit
CAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCTAACAGCA78340.12749696474547806No Hit
ATATCATTTCAACAATCGGTGACTTAGTAAAATGGATTATCGACACAGTG74280.12088938653681527No Hit
ACAAGAAACAGCACACATCAATGAATTAACACCTTAGATTCATCATGAGA73690.11992917196954654No Hit
AAAAATAACTGTAAAACATTCCCTTAATAATAAGTATGGTCGTGAGCCCC69400.11294727282788074No Hit
CAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAAGGAT66770.10866699433310657No Hit
AACTGAATGCTTAGAGCAATCAATTGTTAAAGAAGCTGCGAAAGGTTACT65090.1059328240398668No Hit
AAGTGAGTAACATTTGCTAGTAGAGTTAGTTTCCTTGGACTCAGTGCTAT63730.10371944808819653No Hit
GGATCACCTCCTTTCTAAGGATATATTCGGAACATCTTCTTCAGAAGATG62530.10176646930731097No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)