FastQCFastQC Report
Wed 13 May 2020
D3C.forward.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameD3C.forward.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7324631
Sequences flagged as poor quality0
Sequence length36-325
%GC36

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAACAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCTAAC1394061.9032494606212929No Hit
AAAGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAAGC1027011.4021320664481256No Hit
TCTGGTGACTATAGCAAGGAGGTCACACCTGTTCCCATGCCGAACACAGA695860.9500273802188807No Hit
GTAACACGTGGATAACCTACCTATAAGACTGGGATAACTTCGGGAAACCG615570.8404109367420692No Hit
TCAAACAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCTAA511210.6979327695825224No Hit
AGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAAGGATAT433770.5922073070984736No Hit
GGGGACGTTCATGGATTCGACAGGGGTCCCCCGAGCTCATTAAGCGTGTC329790.45024793740462826No Hit
TTAAAGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAA298040.40690104388876386No Hit
AACAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCTAACAG246270.33622171546935264No Hit
TATGAGTACTTTAACTTATGGATTCAACGGTAATGTTACTGGTGATGATA221680.30265005841249887No Hit
AACACGTGGATAACCTACCTATAAGACTGGGATAACTTCGGGAAACCGGA195580.26701686405772523No Hit
AGTAGAGTTAGTTTCCTTGGACTCAGTGCTATGTATTTTTCTTAATTATC156760.21401760716683202No Hit
AAAACAGACGTTTAGCACAACGTGAAGTTAACAAAGCACCTATGGATGTA154550.21100039032683013No Hit
GATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATAT131280.17923087183504535No Hit
CAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCTAACAGCA127810.17449343181929575No Hit
AATCAAACAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCT123530.16865013404770834No Hit
ACTGAATGACAATATGTCAACGTTAATTCCAAAAACGTAACTATAAGTTA119310.16288875166544226No Hit
CACTAAAAAATAAGATGAATAATTAATTACTTTCATTGTAAATTTGTTAT114770.1566904872067958No Hit
ACTAGATAAGTAAGTAAAATATAGATTTTACCAAGCAAAACCGAGTGAAT112080.15301794725222337No Hit
AAACAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCTAACA109900.1500416881068821No Hit
AGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAAGCTT106680.14564556221330466No Hit
ATATCATTTCAACAATCGGTGACTTAGTAAAATGGATTATCGACACAGTG104380.14250547228932078No Hit
AGTAGAGTTAGTTTCCTTGGACTCAGTGCTATGTATTTTCTTAATTATCA97150.1326346678761019No Hit
AACTGAATGCTTAGAGCAATCAATTGTTAAAGAAGCTGCGAAAGGTTACT93560.12773339708170964No Hit
GAAGGAGTGATTTCAATGGCACAAGATATCATTTCAACAATCGGTGACTT91310.12466156998216019No Hit
AAAAATAACTGTAAAACATTCCCTTAATAATAAGTATGGTCGTGAGCCCC89250.12184914161546159No Hit
CAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAAGGAT88700.12109825054668283No Hit
TAAAGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAAG86690.11835408500441866No Hit
ACCAGACTTCGCTACAGTTATTACTATGGATAGAAAAGCATCCAAACAAC82660.11285210135500341No Hit
AAGTGAGTAACATTTGCTAGTAGAGTTAGTTTCCTTGGACTCAGTGCTAT82610.11278383853056899No Hit
ACAAGAAACAGCACACATCAATGAATTAACACCTTAGATTCATCATGAGA78700.10744568565979637No Hit
ATTAAAGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAA77550.10587564069780442No Hit
GGATCACCTCCTTTCTAAGGATATATTCGGAACATCTTCTTCAGAAGATG77110.10527492784278145No Hit
TAACCATGTAAAATTAGCAAGTGAGTAACATTTGCTAGTAGAGTTAGTTT76360.10425098547626493No Hit
GCATTAAGTGGATCATCATATGTGCCGTAGGGTAGCCGAGATTTAGCTAA75730.10339087388839109No Hit
TGAGTAACACGTGGATAACCTACCTATAAGACTGGGATAACTTCGGGAAA74710.10199831226992868No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)