FastQCFastQC Report
Wed 13 May 2020
D3B.forward.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameD3B.forward.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7087899
Sequences flagged as poor quality0
Sequence length36-326
%GC36

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAACAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCTAAC1360081.9188761013665685No Hit
AAAGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAAGC1083471.5286194117608054No Hit
GTAACACGTGGATAACCTACCTATAAGACTGGGATAACTTCGGGAAACCG581360.8202148478695872No Hit
TCAAACAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCTAA559410.7892465736320452No Hit
GGGGACGTTCATGGATTCGACAGGGGTCCCCCGAGCTCATTAAGCGTGTC535300.7552308519068909No Hit
TCTGGTGACTATAGCAAGGAGGTCACACCTGTTCCCATGCCGAACACAGA509800.7192540412892453No Hit
TTAAAGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAA354650.5003598386489424No Hit
AGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAAGGATAT325490.4592192975661758No Hit
TATGAGTACTTTAACTTATGGATTCAACGGTAATGTTACTGGTGATGATA258810.3651434649393283No Hit
AACAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCTAACAG227810.3214069500708179No Hit
AAAACAGACGTTTAGCACAACGTGAAGTTAACAAAGCACCTATGGATGTA200900.2834408334543142No Hit
AACACGTGGATAACCTACCTATAAGACTGGGATAACTTCGGGAAACCGGA180860.2551672928747997No Hit
GATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATAT139100.19624997478096118No Hit
AATCAAACAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCT132060.1863175533398543No Hit
GAAGGAGTGATTTCAATGGCACAAGATATCATTTCAACAATCGGTGACTT131540.185583908574318No Hit
AGTAGAGTTAGTTTCCTTGGACTCAGTGCTATGTATTTTTCTTAATTATC129990.18339708283089248No Hit
ACTAGATAAGTAAGTAAAATATAGATTTTACCAAGCAAAACCGAGTGAAT127120.17934792806725944No Hit
CACTAAAAAATAAGATGAATAATTAATTACTTTCATTGTAAATTTGTTAT116740.16470324986290014No Hit
TAACCATGTAAAATTAGCAAGTGAGTAACATTTGCTAGTAGAGTTAGTTT115890.16350402284231194No Hit
CAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCTAACAGCA113220.1597370391423467No Hit
TAAAGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAAG104060.14681360442636104No Hit
ACTGAATGACAATATGTCAACGTTAATTCCAAAAACGTAACTATAAGTTA103500.1460235254480912No Hit
AAAAATAACTGTAAAACATTCCCTTAATAATAAGTATGGTCGTGAGCCCC99490.1403659956215516No Hit
AAATGGAAGTTTGAATCAAAAAACATTAAATGTACCCATGACTTAAATTT91230.12871233069207108No Hit
AGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAAGCTT87920.12404239958836885No Hit
AGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAAGCTTCGA85890.1211783632921406No Hit
ACAAGAAACAGCACACATCAATGAATTAACACCTTAGATTCATCATGAGA85840.12110782052622364No Hit
AAGTGAGTAACATTTGCTAGTAGAGTTAGTTTCCTTGGACTCAGTGCTAT85520.12065634682435515No Hit
AAACAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCTAACA84480.11918905729328255No Hit
ATATCATTTCAACAATCGGTGACTTAGTAAAATGGATTATCGACACAGTG84160.11873758359141405No Hit
AACTGAATGCTTAGAGCAATCAATTGTTAAAGAAGCTGCGAAAGGTTACT83090.11722796840079126No Hit
AATGAATCCTGTCGCTAATGCCGCAGATTCTGATATTAATATTAAAACCG82690.11666362627345565No Hit
AGTAGAGTTAGTTTCCTTGGACTCAGTGCTATGTATTTTCTTAATTATCA79630.11234640899933816No Hit
AACCATGTAAAATTAGCAAGTGAGTAACATTTGCTAGTAGAGTTAGTTTC78070.11014547470272927No Hit
AAACAATTTTAGAGAGCCCAACTGATAAAAAAGTAGGCTGGAAAGTGATA77390.10918609308625872No Hit
CAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAAGGAT74880.1056448462372277No Hit
AGATCACAGAGATGTGATGGAAAATAGTTGATGAGTTGTTTAATTTTAAG74400.10496763568442496No Hit
ATTAAAGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAA72630.10247042177096485No Hit
AGGAAACCCTGTAGGTACATTAACTGAATGCTTAGAGCAATCAATTGTTA71820.10132762896311023No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)