FastQCFastQC Report
Wed 13 May 2020
D3A.forward.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameD3A.forward.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7363685
Sequences flagged as poor quality0
Sequence length36-321
%GC35

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAAGC893031.212748779992626No Hit
CAAACAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCTAAC832331.1303172256825218No Hit
GTAACACGTGGATAACCTACCTATAAGACTGGGATAACTTCGGGAAACCG632890.8594745701370985No Hit
TCTGGTGACTATAGCAAGGAGGTCACACCTGTTCCCATGCCGAACACAGA384550.5222249457981976No Hit
AGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAAGGATAT383310.52054100630323No Hit
TCAAACAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCTAA365120.4958387003246336No Hit
GGGGACGTTCATGGATTCGACAGGGGTCCCCCGAGCTCATTAAGCGTGTC352720.47899930537495833No Hit
TTAAAGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAA331610.4503315934888578No Hit
TATGAGTACTTTAACTTATGGATTCAACGGTAATGTTACTGGTGATGATA240310.32634475809326446No Hit
AACACGTGGATAACCTACCTATAAGACTGGGATAACTTCGGGAAACCGGA199670.2711549991614253No Hit
ACTAGATAAGTAAGTAAAATATAGATTTTACCAAGCAAAACCGAGTGAAT165670.22498246462199292No Hit
AACAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCTAACAG160020.21730967579411667No Hit
AAAACAGACGTTTAGCACAACGTGAAGTTAACAAAGCACCTATGGATGTA140990.19146663660925203No Hit
ACTGAATGACAATATGTCAACGTTAATTCCAAAAACGTAACTATAAGTTA138890.1886148035935812No Hit
GAAGGAGTGATTTCAATGGCACAAGATATCATTTCAACAATCGGTGACTT130990.1778864793917719No Hit
CACTAAAAAATAAGATGAATAATTAATTACTTTCATTGTAAATTTGTTAT125250.17009146914893833No Hit
AGTAGAGTTAGTTTCCTTGGACTCAGTGCTATGTATTTTTCTTAATTATC124650.16927665971588954No Hit
TAACCATGTAAAATTAGCAAGTGAGTAACATTTGCTAGTAGAGTTAGTTT122550.16642482670021871No Hit
AAAAATAACTGTAAAACATTCCCTTAATAATAAGTATGGTCGTGAGCCCC116220.1578285871815538No Hit
AAATGGAAGTTTGAATCAAAAAACATTAAATGTACCCATGACTTAAATTT115070.1562668691015436No Hit
AATCAAACAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCT94220.12795224130309757No Hit
AGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAAGCTTCGA91890.12478806467142471No Hit
AACCATGTAAAATTAGCAAGTGAGTAACATTTGCTAGTAGAGTTAGTTTC89040.12091771986444287No Hit
CAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAAGGAT87550.11889427643903834No Hit
AAGTGAGTAACATTTGCTAGTAGAGTTAGTTTCCTTGGACTCAGTGCTAT83600.1135301143381337No Hit
ACAAGAAACAGCACACATCAATGAATTAACACCTTAGATTCATCATGAGA83140.11290542710612961No Hit
ATATCATTTCAACAATCGGTGACTTAGTAAAATGGATTATCGACACAGTG83110.11286468663447716No Hit
AACTGAATGCTTAGAGCAATCAATTGTTAAAGAAGCTGCGAAAGGTTACT81750.11101778525289988No Hit
AGGAAACCCTGTAGGTACATTAACTGAATGCTTAGAGCAATCAATTGTTA81130.11017581550541611No Hit
TAAAGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAAG81080.11010791471932871No Hit
AGTAGAGTTAGTTTCCTTGGACTCAGTGCTATGTATTTTCTTAATTATCA78070.10602028739686718No Hit
GATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATAT77150.10477091293285903No Hit
GGATCACCTCCTTTCTAAGGATATATTCGGAACATCTTCTTCAGAAGATG76270.10357585909772078No Hit
TGAGTAACACGTGGATAACCTACCTATAAGACTGGGATAACTTCGGGAAA74610.10132155299961909No Hit
AATGAATCCTGTCGCTAATGCCGCAGATTCTGATATTAATATTAAAACCG74030.10053390388100522No Hit
CAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCTAACAGCA73660.10003143806395848No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)