FastQCFastQC Report
Wed 13 May 2020
D17_CIPC.forward.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameD17_CIPC.forward.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6578079
Sequences flagged as poor quality0
Sequence length36-312
%GC36

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAAGC1093441.6622482034648718No Hit
TCTGGTGACTATAGCAAGGAGGTCACACCTGTTCCCATGCCGAACACAGA878941.3361651631122093No Hit
AGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAAGGATAT524960.798044535494329No Hit
GGGGACGTTCATGGATTCGACAGGGGTCCCCCGAGCTCATTAAGCGTGTC524410.7972084251344503No Hit
CAAACAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCTAAC296100.45013141374556315No Hit
TTAAAGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAA245900.37381734089845986No Hit
GTAACACGTGGATAACCTACCTATAAGACTGGGATAACTTCGGGAAACCG188600.2867098434056508No Hit
AGTAGAGTTAGTTTCCTTGGACTCAGTGCTATGTATTTTTCTTAATTATC165220.2511675521075378No Hit
TATGAGTACTTTAACTTATGGATTCAACGGTAATGTTACTGGTGATGATA128040.19464649177974297No Hit
TCAAACAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCTAA114630.1742606010052479No Hit
AAACAGGGCACCCACCTGTATATAACAGGCCGAATGATCAAGCTATTATA110460.1679213642767136No Hit
GGATCACCTCCTTTCTAAGGATATATTCGGAACATCTTCTTCAGAAGATG107210.1629807121501581No Hit
ACTGAATGACAATATGTCAACGTTAATTCCAAAAACGTAACTATAAGTTA106360.16168854159398208No Hit
AGTAGAGTTAGTTTCCTTGGACTCAGTGCTATGTATTTTCTTAATTATCA95960.1458784547890045No Hit
ACCAGACTTCGCTACAGTTATTACTATGGATAGAAAAGCATCCAAACAAC90520.1376085632294778No Hit
ATATCATTTCAACAATCGGTGACTTAGTAAAATGGATTATCGACACAGTG89670.13631639267330173No Hit
AAGTGAGTAACATTTGCTAGTAGAGTTAGTTTCCTTGGACTCAGTGCTAT87800.13347361744971442No Hit
AGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAAGCTT87690.1333063953777387No Hit
AAAAATAACTGTAAAACATTCCCTTAATAATAAGTATGGTCGTGAGCCCC86980.1322270529131681No Hit
TACTAAACGTAGATGGAACGCTAACCTTCAAAAAGTTAGAATCCTAGTTG78480.11930534735140762No Hit
AACACGTGGATAACCTACCTATAAGACTGGGATAACTTCGGGAAACCGGA75410.11463833134263057No Hit
AACTGAATGCTTAGAGCAATCAATTGTTAAAGAAGCTGCGAAAGGTTACT74810.11372621095003571No Hit
CAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAAGGAT74460.11319414072102205No Hit
AGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAAGCTTCGA74350.11302691864904632No Hit
CACTAAAAAATAAGATGAATAATTAATTACTTTCATTGTAAATTTGTTAT73680.11200838421064874No Hit
TATGGATTCAACGGTAATGTTACTGGTGATGATACAGGAAAAATTGGCGG71960.10939363908521013No Hit
TAAAGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAAG70150.1066420759008823No Hit
GGGGGCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAG69800.10611000567186864No Hit
AGTGTATTAAATATGAGTAATCAACAAGATGTTGTAAAAGAATTGAATCA69530.10569955149520094No Hit
CAAGCAAAACCGAGTGAATAAAGAGTTTTAAATAAGCTTGAATTCATAAG68960.10483303712223582No Hit
ATAAGAAATAATCGCTAGTGTTCGAAAGAACACTCACAAGATTAATAACG67650.10284157426507039No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)