FastQCFastQC Report
Wed 13 May 2020
D17_CIPB.forward.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameD17_CIPB.forward.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6083532
Sequences flagged as poor quality0
Sequence length36-312
%GC36

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAAGC1138221.8709854735702878No Hit
GGGGACGTTCATGGATTCGACAGGGGTCCCCCGAGCTCATTAAGCGTGTC818251.345024567964794No Hit
TCTGGTGACTATAGCAAGGAGGTCACACCTGTTCCCATGCCGAACACAGA723081.1885858412514307No Hit
AGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAAGGATAT413570.6798188946815764No Hit
CAAACAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCTAAC278930.4585000950105958No Hit
TTAAAGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAA252240.41462755517682814No Hit
AGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAAGCTTCGA157890.2595367296498153No Hit
GTAACACGTGGATAACCTACCTATAAGACTGGGATAACTTCGGGAAACCG134060.22036540614892797No Hit
AGTAGAGTTAGTTTCCTTGGACTCAGTGCTATGTATTTTTCTTAATTATC132800.2182942409113653No Hit
AAACAGGGCACCCACCTGTATATAACAGGCCGAATGATCAAGCTATTATA127670.2098616395870031No Hit
ACTGAATGACAATATGTCAACGTTAATTCCAAAAACGTAACTATAAGTTA108120.17772570276609048No Hit
TATGAGTACTTTAACTTATGGATTCAACGGTAATGTTACTGGTGATGATA104100.1711176993891049No Hit
AGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAAGCTT102570.16860271302920737No Hit
TCAAACAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCTAA101730.16722193620416562No Hit
GGATCACCTCCTTTCTAAGGATATATTCGGAACATCTTCTTCAGAAGATG82300.13528325321540183No Hit
TAAAGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAAG79150.13010534012149522No Hit
AAGTGAGTAACATTTGCTAGTAGAGTTAGTTTCCTTGGACTCAGTGCTAT75100.1234480232864724No Hit
ACCAGACTTCGCTACAGTTATTACTATGGATAGAAAAGCATCCAAACAAC73790.12129466895218106No Hit
GCATTAAGTGGATCATCATATGTGCCGTAGGGTAGCCGAGATTTAGCTAA73090.12014402159797959No Hit
AAAAATAACTGTAAAACATTCCCTTAATAATAAGTATGGTCGTGAGCCCC71060.1168071442707953No Hit
AGTAGAGTTAGTTTCCTTGGACTCAGTGCTATGTATTTTCTTAATTATCA69460.11417709317547767No Hit
TACTAAACGTAGATGGAACGCTAACCTTCAAAAAGTTAGAATCCTAGTTG68520.11263193815697856No Hit
AACACGTGGATAACCTACCTATAAGACTGGGATAACTTCGGGAAACCGGA66190.10880192624942221No Hit
ATTAAAGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAA66060.10858823459792766No Hit
AACAGGGCACCCACCTGTATATAACAGGCCGAATGATCAAGCTATTATAA62530.10278568436888308No Hit
CAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAAGGAT61420.10096108642150645No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)